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1.
Genet. mol. res. (Online) ; 2(4): 383-393, Dec. 2003.
Artigo em Inglês | LILACS | ID: lil-417591

RESUMO

Most organisms grow at temperatures from 20 to 50 degrees C, but some prokaryotes, including Archaea and Bacteria, are capable of withstanding higher temperatures, from 60 to >100 degrees C. Their biomolecules, especially proteins, must be sufficiently stable to function under these extreme conditions; however, the basis for thermostability remains elusive. We investigated the preferential usage of certain groupings of amino acids and codons in thermally adapted organisms, by comparative proteome analysis, using 28 complete genomes from 18 mesophiles (M), 4 thermophiles (T), and 6 hyperthermophiles (HT). Whenever the percent of glutamate (E) and lysine (K) increased in the HT proteomes, the percent of glutamine (Q) and histidine (H) decreased, so that the E + K/Q + H ratio was >4.5; it was <2.5 in the M proteomes, and 3.2 to 4.6 in T. The E + K/Q + H ratios for chaperonins, potentially thermostable proteins, were higher than their proteome ratios, whereas for DNA ligases, which are not necessarily thermostable, they followed the proteome ratios. Analysis of codon usage revealed that HT had more AGR codons for Arg than they did CGN codons, which were more common in mesophiles. The E + K/Q + H ratio may provide a useful marker for distinguishing HT, T and M prokaryotes, and the high percentage of the amino acid couple E + K, consistently associated with a low percentage of the pair Q + H, could contribute to protein thermostability. The preponderance of AGR codons for Arg is a signature of all HT so far analyzed. The E + K/Q + H ratio and the codon bias for Arg are apparently not related to phylogeny. HT members of the Bacteria show the same values as the HT members of the Archaea; the values for T organisms are related to their lifestyle (intermediate temperature) and not to their domain (Archaea) and the values for M are similar in Eukarya, Bacteria and Archaea


Assuntos
Aminoácidos/genética , Archaea/crescimento & desenvolvimento , Bactérias/crescimento & desenvolvimento , Temperatura Alta , Adaptação Biológica , Archaea/química , Archaea/genética , Bactérias/química , Bactérias/genética , DNA Ligases/análise , DNA Ligases/genética , Proteínas de Bactérias/genética , Proteoma/análise , Proteoma/genética
3.
Biotecnol. apl ; 7(2): 204-12, mayo-ago. 1990. tab
Artigo em Espanhol | LILACS | ID: lil-97067

RESUMO

El procedimiento reportado por Davis et al., 1980. para purificar T4 ADN ligasa, ha sido modificado con el objetivo de obtener una preparación de la enzima virtualmente libre de exonucleasas. Se modificaron las condiciones de elución de las columnas de P11 e hidroxilapatita: en vez de eludir en un paso, en ambos se aplicó un gradiente lineal, de 300 a 800 mM de cloruro de sodio en la columna de P11 y de 0 a 800 mM en la de hidroxilapatita. Este procedimiento permitió la eliminación de nucleasas y la obtención de una preparación enzimática de gran calidad


Assuntos
DNA Ligases/isolamento & purificação , Exonucleases
4.
Rev. microbiol ; 13(2): 101-9, 1982.
Artigo em Inglês | LILACS | ID: lil-9900

RESUMO

Sistemas de reparo do DNA tem merecido atencao, principalmente devido ao papel que exercem em mutagenese e carcinogenese. Sao revistos fatos ja estabelecidos e avancos recentes, no assunto, com enfase nos eventos enzimaticos, envolvidos nas celulas bacterianas


Assuntos
DNA Ligases , Dímeros de Pirimidina , Reparo do DNA
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