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1.
Periodontia ; 28(2): 24-31, 2018. tab
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-908854

RESUMO

A periodontite é uma doença inflamatória, que pode ser classificada em Periodontite Crônica (PC) ou Periodontite Agressiva (PA), desencadeada por um desequilíbrio na microbiota subgengival, que pode ser influenciado por diversos fatores, como polimorfismos genéticos. Dessa forma, o objetivo deste trabalho foi analisar, através de uma revisão da literatura, se há ou não associação entre a periodontite e polimorfismos genéticos de nucleotídeo único (SNPs). Os genes IL1B +3954(3), -511, -31 foram os alvos desta pesquisa, por serem os mais estudados e apresentarem boa plausibilidade biológica. Foi realizada uma busca no PubMed e ao final 24 artigos de estudos casos-controle foram selecionados. Na maioria dos estudos não foi encontrada associação positiva entre os SNPs +3954(3), -511, -31 da IL1B e a PA ou PC. Dessa forma, é possível concluir que não há associação positiva entre a periodontite e os SNPs IL1B +3954(3), -511, -31 e PA e PC. Todavia os resultados devem ser analisados com cautela, pois os estudos apresentam limitações. (AU)


Periodontitis is an inflammatory disease which is classified as chronic periodontitis (PC) or aggressive periodontitis (PA) and is initiated by an unbalance in subgengival microbiota which for their part can be influenced by a lot of factors, such as genetic polymorphism. Therefore, the aim of this study was to analyse if there is an association between periodontitis and single nucleotide polymorphisms (SNPs). The genes IL1B +3954(3), -511, -31 were chosen as the targets of this literature review, because they have good biologic plausibility and are the most studied in literature. A search was conducted on PubMed and the results analysed and 24 case-controls articles were chosen.Most of the studies did not find a positive association between the ILB1 SNPs +3954(3), -511, -31 and PA e PC. Therefore, the case-controls studies indicated that there is no positive association between SNPs IL1B +3954(3), -511, -31 and PA or PC. However, the results of this work must be analysed carefully as the studies used have limitations. (AU)


Assuntos
Periodontite , Periodontite Agressiva , Variação Genética , Interleucina-1 , Predisposição Genética para Doença , Polimorfismo de Nucleotídeo Único/genética , Suscetibilidade a Doenças , Interleucina-1alfa , Interleucina-1beta , Periodontite Crônica , Genótipo
2.
Braz. oral res. (Online) ; 30(1): e78, 2016. graf
Artigo em Inglês | LILACS | ID: biblio-951984

RESUMO

Abstract The aim of this study was to compare potential aspects of periapical lesion formation in hypertensive and normotensive conditions using hypertensive (BPH/2J) and wild-type control (BPN/3J) mice. The mandibular first molars of both strains had their dental pulp exposed. At day 21 the mice were euthanized and right mandibular molars were used to evaluate the size and phenotype of apical periodontitis by microCT. Proteins were extracted from periapical lesion on the left side and the expressions of IL1α, IL1β and TNFα were analyzed by ELISA. Bone marrow stem cells were isolated from adult mice femurs from 2 strains and osteoclast differentiation was evaluated by tartrate-resistant acid phosphatase (TRAP) in vitro. The amount of differentiated osteoclastic cells was nearly double in hypertensive mice when compared to the normotensive strain (p < 0.03). Periapical lesion size did not differ between hypertensive and normotensive strains (p > 0.7). IL1α, IL1β and TNFα cytokines expressions were similar for both systemic conditions (p > 0.05). Despite the fact that no differences could be observed in periapical lesion size and cytokines expressions on the systemic conditions tested, hypertension showed an elevated number of osteoclast differentiation.


Assuntos
Animais , Masculino , Feminino , Camundongos , Doenças Periapicais/patologia , Células da Medula Óssea/patologia , Ligante RANK/análise , Hipertensão/patologia , Doenças Periapicais/etiologia , Valores de Referência , Fatores de Tempo , Ensaio de Imunoadsorção Enzimática , Fator de Necrose Tumoral alfa/análise , Interleucina-1alfa/análise , Interleucina-1beta/análise , Microtomografia por Raio-X , Fosfatase Ácida Resistente a Tartarato , Hipertensão/complicações
3.
Trends psychiatry psychother. (Impr.) ; 37(3): 143-151, jul. set. 2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-764667

RESUMO

Objective:To investigate the effects of ethanol exposure in adolescent rats during adulthood by assesssing aggression and anxiety-like behaviors and measuring the levels of inflammatory markers.Methods:Groups of male Wistar rats (mean weight 81.4 g, n = 36) were housed in groups of four until postnatal day (PND) 60. From PNDs 30 to 46, rats received one of three treatments: 3 g/kg of ethanol (15% w/v, orally, n = 16), 1.5 g/kg of ethanol (12.5% w/v, PO, n = 12), or water (n = 12) every 48 hours. Animals were assessed for aggressive behavior (resident x intruder test) and anxiety-like behaviors (elevated plus maze) during adulthood.Results:Animals that received low doses of alcohol showed reduced levels of brain-derived neurotrophic factor (BDNF) in the hippocampus as compared to the control group. No significant difference was found in prefrontal cortex.Conclusions:Intermittent exposure to alcohol during adolescence is associated with lower levels of BDNF in the hippocampus, probably due the episodic administration of alcohol, but alcohol use did not alter the level agression toward a male intruder or anxiety-like behaviors during the adult phase.


Objetivo: Investigar os efeitos da exposição ao etanol em ratos adolescentes durante a idade adulta sobre os comportamentos agressivos e semelhantes à ansiedade, bem como sobre as medidas de níveis de marcadores inflamatórios.Métodos:Os grupos de ratos Wistar machos (peso médio de 81,4 g; n = 36) foram alojados em grupos de quatro até o dia pós-natal (DPN) 60. Entre os DPNs 30 e 46, os ratos receberam um dos três tratamentos: 3 g/kg de etanol (15% w/v, oralmente, n = 16), 1.5 g/kg de etanol (12,5% w/v, oralmente, n = 12), ou água (n = 12) a cada 48 horas. Os comportamentos agressivos (teste residente-intruso) e semelhantes à ansiedade (labirinto em cruz elevado) foram avaliados durante a idade adulta dos animais.Resultados:Os animais que receberam doses menores de álcool mostraram níveis reduzidos de fator neurotrófico derivado do cérebro (BDNF) no hipocampo quando comparados ao grupo controle. Nenhuma diferença significativa foi verificada no córtex pré-frontal.Conclusões:A exposição intermitente ao álcool durante a adolescência é associada com menores níveis de BDNF no hipocampo, provavelmente divido a administração episódica de álcool, mas o uso não alterou o nível de agressão contra o macho intruso ou os comportamentos semelhantes à ansiedade durante a fase adulta.


Assuntos
Animais , Masculino , Depressores do Sistema Nervoso Central/administração & dosagem , Etanol/administração & dosagem , Consumo Excessivo de Bebidas Alcoólicas/metabolismo , Consumo Excessivo de Bebidas Alcoólicas/psicologia , Hipocampo/crescimento & desenvolvimento , Hipocampo/efeitos dos fármacos , Ansiedade/fisiopatologia , Assunção de Riscos , Depressores do Sistema Nervoso Central/efeitos adversos , Fator de Necrose Tumoral alfa/metabolismo , Interleucina-10/metabolismo , Ratos Wistar , Córtex Pré-Frontal/crescimento & desenvolvimento , Córtex Pré-Frontal/efeitos dos fármacos , Córtex Pré-Frontal/metabolismo , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Agressão/efeitos dos fármacos , Agressão/fisiologia , Agressão/psicologia , Modelos Animais de Doenças , Etanol/efeitos adversos , Relação Dose-Resposta a Droga , Interleucina-1alfa/metabolismo , Hipocampo/metabolismo
4.
Genet. mol. biol ; 34(1): 1-5, 2011. tab
Artigo em Inglês | LILACS | ID: lil-573700

RESUMO

An inflammatory process has been involved in numerous neurodegenerative disorders such as Parkinson's disease, stroke and Alzheimer's disease (AD). In AD, the inflammatory response is mainly located in the vicinity of amyloid plaques. Cytokines, such as interleukin-8 (IL-8) and interleukin-1α (IL-1α), have been clearly involved in this inflammatory process. Polymorphisms of several interleukin genes have been correlated to the risk of developing AD. The present study investigated the association of AD with polymorphisms IL-8 -251T > A (rs4073) and IL-1α-889C > T (rs1800587) and the interactive effect of both, adjusted by the Apolipoprotein E genotype. 199 blood samples from patients with AD, 146 healthy elderly controls and 95 healthy young controls were obtained. DNA samples were isolated from blood cells, and the PCR-RFLP method was used for genotyping. The genotype distributions of polymorphisms IL-8, IL-1α and APOE were as expected under Hardy-Weinberg equilibrium. The allele frequencies did not differ significantly among the three groups tested. As expected, the APOE4 allele was strongly associated with AD (p < 0.001). No association of AD with either the IL-1α or the IL-8 polymorphism was observed, nor was any interactive effect between both polymorphisms. These results confirm previous studies in other populations, in which polymorphisms IL-8 -251T > A and IL-1α-889C > T were not found to be risk factors for AD.


Assuntos
Humanos , Masculino , Feminino , Doença de Alzheimer , Apolipoproteínas E , Interleucina-1alfa , Interleucina-8
5.
Braz. oral res ; 23(4): 452-459, Oct.-Dec. 2009. tab, graf
Artigo em Inglês | LILACS | ID: lil-534222

RESUMO

This study investigated the association of IL-1A (+4845) and IL-1B (+3954) gene polymorphism with the subgingival microbiota and periodontal status of HIV-infected Brazilian individuals on highly active antiretroviral therapy (HAART). One hundred and five subjects were included in the study, distributed into 2 HIV groups [29 chronic periodontitis (CP+) and 30 periodontally healthy (H+)]; and 2 non-HIV groups (29 CP- and 17 H- patients). IL-1A and B were genotyped by PCR and restriction enzyme digestion. Thirty-three bacterial species were detected by checkerboard. Overall, we observed a prevalence of the allele 2 in the IL1-A and IL-1B polymorphism at 30.5 percent and 25.7 percent, respectively. Only 11.4 percent of all patients were composite genotype-positive, and 75 percent of those were HIV-infected. No significant associations between polymorphism of the IL-1 gene and periodontitis or HIV infection were observed. Likewise, no significant differences in the frequency and counts of any bacterial species were found between individuals with and without allele 2 (IL-1A or IL-1B). The data indicated that the IL-1 gene polymorphism is neither associated with periodontal destruction nor with high levels of subgingival species, including putative periodontal pathogens in HIV Brazilian individuals on HAART.


Assuntos
Humanos , Terapia Antirretroviral de Alta Atividade , Periodontite Crônica/microbiologia , Gengiva/microbiologia , Infecções por HIV/tratamento farmacológico , Interleucina-1/genética , Polimorfismo Genético , Brasil , Bactérias/classificação , Métodos Epidemiológicos , Genótipo , Interleucina-1alfa/genética , Interleucina-1beta/genética , Reação em Cadeia da Polimerase
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