Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 17 de 17
Filtrar
Mais filtros










Filtros aplicados
Intervalo de ano de publicação
1.
Int. j. morphol ; 38(5): 1398-1404, oct. 2020. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1134455

RESUMO

SUMMARY: The objective of this study were bone defect complications that occur due to traumas or infections. Bone grafts are required to provide support, fill gaps and improve biological repair in skeletal damage. Dexamethasone plays role in calcium signaling modulation and used in diseases. Aim of this study was to evaluate osteonectin and osteopontin expressions in new bone development after dexamethasone application on tibial bone defects. Rats were divided into defect, defect+graft and defect+graft+dexamethasone treated groups. Tibial bone defect created, and rats were kept immobile for 28 days. Alloplastic material was placed in defect area in second and group third groups. 2.5 mg/kg Dex and normal saline were injected to dexamethasone and defect groups twice a week for 56 days. Inflammation and congestion were increased in defect and defect+graft groups. Defect+graft+dexamethasone group; increased number of osteoblast and osteocyte cells, dense bone matrix, formation of new bone trabeculae was observed. Defect+graft group; osteonectin expression in graft regions, osteoblast cells, some connective tissue cells and fibers were seen whereas in defect+graft+dexamethasone group; osteopontin expression in osteoblast and osteocyte cells of new bone trabeculae were observed. Dexamethasone may lead to formation of new bone trabeculae into the graft material resulting in increased osteoconduction and osteoinductive effect for differentiation of osteon.


RESUMEN: Los defectos óseos son complicaciones que ocurren debido a traumas o infecciones. Se requieren injertos óseos para proporcionar apoyo, llenar los espacios y mejorar la reparación biológica en el hueso dañado. La dexametasona desempeña un papel importante en la modulación de la señalización del calcio y se usa en enfermedades. El objetivo de este estudio fue evaluar las expresiones de osteonectina y osteopontina en el desarrollo óseo después de la aplicación de dexametasona en defectos óseos tibiales. Las ratas se dividieron en grupos: defecto, defecto + injerto y defecto + injerto + grupos tratados con dexametasona. Se creó un defecto óseo tibial, y las ratas se mantuvieron inmóviles durante 28 días. El material aloplástico se colocó en el área del defecto en el segundo y tercer grupo. Se inyectaron 2,5 mg / kg de dexametasona y solución salina normal a grupos de defectos dos veces por semana durante 56 días. La inflamación y la congestión aumentaron en los grupos de defectos y defectos + injerto; En el grupo defecto + injerto + grupo tratado con dexametasona se observó un aumento en el número de osteoblastos y osteocitos, de matriz ósea densa y en la formación de nuevas trabéculas óseas. En el grupo defecto + grupo de injerto se observó la expresión de osteonectina en las áreas de injerto, osteoblastos, algunas células y fibras de tejido conectivo, mientras que en el grupo defecto + injerto + dexametasona se observó la expresión de osteopontina en osteoblastos y osteocitos y formación de nuevas trabéculas óseas . En conclusión la dexametasona puede conducir a la formación de nuevas trabéculas óseas en el material de injerto, lo que resulta en un aumento de la osteoconducción y un efecto osteoinductivo para la diferenciación del osteón.


Assuntos
Animais , Masculino , Ratos , Tíbia/cirurgia , Tíbia/efeitos dos fármacos , Dexametasona/administração & dosagem , Transplante Ósseo , Tíbia/patologia , Regeneração Óssea , Imuno-Histoquímica , Osteonectina/fisiologia , Remodelação Óssea , Ratos Wistar , Modelos Animais de Doenças , Osteopontina/fisiologia
2.
Acta cir. bras ; 34(7): e201900704, 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1038112

RESUMO

Abstract Purpose: The effects of resveratrol administration on calvarial bone defects with alloplastic graft material was investigated for osteoinductive reaction and bone development in rats. Methods: Healthy male rats were randomly divided into 3 groups consisting of 10 rats. Groups were as follows: control (defect) group, defect + graft group, and defect + graft + resveratrol group. A calvarial bone defect was created in all groups, alloplastic bone grafts were applied to the defect in the 2nd and 3rd group, resveratrol (5 mg/kg/day) was added to the drinking water of the animals following graft application for 28 days in the 3rd group. Results: Increase in osteoclasts and necrotic changes were observed histopathologically in the control group. In the 2nd group, reduction of inflammation, congestion of blood vessels, increased osteblastic activity, osteoinductive effect, progression of osteocyte development and increased collagen fibers in connective tissue were observed. In the 3rd group, osteoblasts seemed to secrete bone matrix and accelerate osteoinductive effect with increased osteopregenitor activity and positive osteopontin and osteonectin expressions. Conclusion: Resveratrol treatment was thought to be an alternative and supportive drug for implant application by inducing new bone formation in the calvaral defect region as a result of short-term treatment.


Assuntos
Animais , Masculino , Ratos , Crânio/cirurgia , Regeneração Óssea/efeitos dos fármacos , Transplante Ósseo/métodos , Substitutos Ósseos/administração & dosagem , Resveratrol/administração & dosagem , Osteoblastos/efeitos dos fármacos , Osteogênese/efeitos dos fármacos , Crânio/efeitos dos fármacos , Esquema de Medicação , Osteonectina/administração & dosagem , Osseointegração/efeitos dos fármacos , Substitutos Ósseos/uso terapêutico , Modelos Animais de Doenças , Osteopontina/administração & dosagem
3.
J. appl. oral sci ; 27: e20180317, 2019. tab, graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-984571

RESUMO

Abstract Bone morphogenetic protein type 2 (BMP-2) and retinoic acid (RA) are osteoinductive factors that stimulate endogenous mechanisms of bone repair which can be applied on management of osseous defects in oral and maxillofacial fields. Objective Considering the different results of RA on osteogenesis and its possible use to substitute/potency BMP-2 effects, this study evaluated the outcomes of BMP-2, RA, and BMP-2+RA treatments on in vitro osteogenic differentiation of human adipose-derived stem cells (ASCs) and the signaling pathway(s) involved. Material and Methods ASCs were treated every other day with basic osteogenic medium (OM) alone or supplemented with BMP-2, RA, or BMP-2+RA. Alkaline phosphatase (ALP) activity was determined using the r-nitrophenol method. Extracellular matrix mineralization was evaluated using von Kossa staining and calcium quantification. Expression of osteonectin and osteocalcin mRNA were determined using qPCR. Smad1, Smad4, phosphorylated Smad1/5/8, BMP-4, and BMP-7 proteins expressions were analyzed using western blotting. Signaling pathway was evaluated using the IPA® software. Results RA promoted the highest ALP activity at days 7, 14, 21, and 28, in comparison to BMP-2 and BMP-2+RA. BMP-2+RA best stimulated phosphorylated Smad1/5/8 protein expression at day 7 and Smad4 expression at days 7, 14, 21, and 28. Osteocalcin and osteonectin mRNA expressions were best stimulated by BMP-2+RA at day 7. Matrix mineralization was most improved by BMP-2+RA at days 12 and 32. Additionally, BMP-2+RA promoted the highest BMP signaling pathway activation at days 7 and 14, and demonstrated more activation of differentiation of bone-forming cells than OM alone. Conclusions In summary, RA increased the effect of BMP-2 on osteogenic differentiation of human ASCs.


Assuntos
Humanos , Osteogênese/efeitos dos fármacos , Tretinoína/farmacologia , Diferenciação Celular/efeitos dos fármacos , Proteína Morfogenética Óssea 2/efeitos dos fármacos , Células-Tronco Mesenquimais/efeitos dos fármacos , Osteoblastos/efeitos dos fármacos , Osteogênese/fisiologia , Valores de Referência , Fatores de Tempo , Osteocalcina/análise , Osteocalcina/efeitos dos fármacos , Osteonectina/análise , Osteonectina/efeitos dos fármacos , Diferenciação Celular/fisiologia , Células Cultivadas , Western Blotting , Reprodutibilidade dos Testes , Análise de Variância , Fosfatase Alcalina/análise , Fosfatase Alcalina/efeitos adversos , Proteína Morfogenética Óssea 2/metabolismo , Células-Tronco Mesenquimais/metabolismo
4.
J. appl. oral sci ; 27: e20180014, 2019. graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-975888

RESUMO

Abstract Stanozolol (ST) is a synthetic androgen with high anabolic potential. Although it is known that androgens play a positive role in bone metabolism, ST action on bone cells has not been sufficiently tested to support its clinical use for bone augmentation procedures. Objective: This study aimed to assess the effects of ST on osteogenic activity and gene expression in SaOS-2 cells. Material and Methods: SaOS-2 deposition of mineralizing matrix in response to increasing doses of ST (0-1000 nM) was evaluated through Alizarin Red S and Calcein Green staining techniques at 6, 12 and 24 days. Gene expression of runt-related transcription factor 2 (RUNX2), vitamin D receptor (VDR), osteopontin (SPP1) and osteonectin (ON) was analyzed by RT-PCR. Results: ST significantly influenced SaOS-2 osteogenic activity: stainings showed the presence of rounded calcified nodules, which increased both in number and in size over time and depending on ST dose. RT-PCR highlighted ST modulation of genes related to osteogenic differentiation. Conclusions: This study provided encouraging results, showing ST promoted the osteogenic commitment of SaOS-2 cells. Further studies are required to validate these data in primary osteoblasts and to investigate ST molecular pathway of action.


Assuntos
Humanos , Osteogênese/efeitos dos fármacos , Estanozolol/farmacologia , Expressão Gênica/efeitos dos fármacos , Anabolizantes/farmacologia , Osteoblastos/efeitos dos fármacos , Fatores de Tempo , Calcificação Fisiológica/efeitos dos fármacos , Modelos Lineares , Osteonectina/análise , Osteonectina/efeitos dos fármacos , Reprodutibilidade dos Testes , Análise de Variância , Receptores de Calcitriol/análise , Receptores de Calcitriol/efeitos dos fármacos , Linhagem Celular Tumoral/efeitos dos fármacos , Subunidade alfa 1 de Fator de Ligação ao Core/análise , Subunidade alfa 1 de Fator de Ligação ao Core/efeitos dos fármacos , Osteopontina/análise , Osteopontina/efeitos dos fármacos , Reação em Cadeia da Polimerase em Tempo Real
5.
Int. j. morphol ; 36(1): 206-211, Mar. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-893212

RESUMO

SUMMARY: The purpose of this study was to investigate effects of diabetes mellitus (DM) on the alveolar bone with histopathological and immunohistochemical methods. Wistar rats were divided into two groups, control and diabetes group. Control group was fed standard rat chow and drinking water for 8 weeks. Single dose (Streptozotocin) STZ (55 mg/kg), was dissolved in sodium citrate buffer and introduced intraperitoneal injection. Diabetes group and control group were compared in terms of glucose values. The blood glucose concentration in diabetic rats was significantly high (p <0.05). In diabetes group; periodontal membrane and the dilation of blood vessels, hemorrhage has also been a significant increase in inflammatory cells. In the diabetes group, osteonectin showed positive expression in periodontal membrane and showed negative expression in osteocytes of alveolar bone. Osteopontin expression in fibroblast cells and periodontal membrane collagen fibrils was positive, alveolar cells, osteocytes and bone matrix bone was found positive. Diabetes results showed that there formed periodontitis; due to the increase in inflammation inhibiting bone formation delaying the development of early bone cells.


RESUMEN: El objetivo de este estudio fue investigar los efectos de la diabetes mellitus (DM) sobre el hueso alveolar con métodos histopatológicos e inmunohistoquímicos. Las ratas Wistar se dividieron en dos grupos, grupo control y grupo de diabetes. El grupo control fue alimentado con comida estándar y agua potable durante 8 semanas. La dosis única Streptozotocina (STZ) (55 mg/ kg), se disolvió en tampón de citrato de sodio y se introdujo mediante inyección intraperitoneal. El grupo diabetes y el grupo control se compararon en términos de valores de glucosa. La concentración de glucosa en sangre en ratas diabéticas fue significativamente alta (p <0,05). En el grupo diabetes hubo un aumento significativo de la membrana periodontal y dilatación de los vasos sanguíneos y hemorragia, con un aumento significativo de células inflamatorias. En el grupo diabetes, la osteonectina mostró una expresión positiva en la membrana periodontal además se observó expresión negativa en los osteocitos del hueso alveolar. La expresión de osteopontina en fibroblastos y fibrillas de colágeno en membrana periodontal fue positiva, las células alveolares, osteocitos y hueso de la matriz ósea dio positivo. Los resultados de la diabetes mostraron que existía periodontitis, debido al aumento de la inflamación que inhibió la formación ósea retardando el desarrollo de células óseas tempranas.


Assuntos
Animais , Ratos , Processo Alveolar/metabolismo , Processo Alveolar/patologia , Diabetes Mellitus Experimental/patologia , Glicemia , Western Blotting , Diabetes Mellitus Experimental/metabolismo , Imuno-Histoquímica , Osteonectina/metabolismo , Osteopontina/metabolismo , Ratos Wistar
6.
J. appl. oral sci ; 25(5): 515-522, Sept.-Oct. 2017. graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-893656

RESUMO

Abstract Hypersensitivity, local irritative and cytotoxic effects are known for the chemical components of Syzygium aromaticum and Cinnamomum zeylanicum contained in dental materials. However, there is no intimate data in dentistry using the whole extracts of these plants and introducing new ones. Salvia triloba is a well-known anti-inflammatory plant that correspondingly could be used in several dental traumas. Objectives: We aimed to show and compare the effect of S. aromaticum, C. zeylanicum, and S. triloba extracts on dental pulp stem cells (DPSCs) proliferation, differentiation, and immune responses. Material and Methods: Using xCELLigence, a real time monitoring system, we obtained a growth curve of DPSCs with different concentrations of the Extracts. A dose of 10 μg/mL was the most efficient concentration for vitality. Osteogenic differentiation and anti-inflammatory activities were determined by using an ELISA Kit to detect early and late markers of differentiation. Results: The level of osteonectin (ON, early osteogenic marker) decreased, which indicated that the osteogenic differentiation may be accelerated with addition of extracts. However, the level of osteocalcin (OCN, late osteogenic marker and sign of calcium granulation) differed among the extracts, in which S. aromaticum presented the highest value, followed by S. triloba and C. zeylanicum. Surprisingly, the determined calcium granules were reduced in S. aromaticum and S. triloba. In response to tumor necrosis factor alpha (TNF-α), S. triloba-treated DPSCs showed the most reduced level of IL-6 cytokine level. We suggest C. zeylanicum as a promising osteogenic inducer and S. triloba as a potent anti-inflammatory agent, which could be used safely in biocomposite or scaffold fabrications for dentistry. Conclusions: Because calcium granule formation and cell viability play a critical role in hard tissue formation, S. aromaticum in dentistry should be strictly controlled, and the mechanism leading to reduced calcium granule formation should be identified.


Assuntos
Humanos , Adolescente , Adulto Jovem , Medicamentos de Ervas Chinesas/farmacologia , Extratos Vegetais/farmacologia , Cinnamomum zeylanicum/química , Syzygium/química , Polpa Dentária/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Anti-Inflamatórios/farmacologia , Osteogênese/efeitos dos fármacos , Fatores de Tempo , Ensaio de Imunoadsorção Enzimática , Antígenos de Diferenciação/análise , Osteocalcina/análise , Osteonectina/análise , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Cálcio/análise , Reprodutibilidade dos Testes , Análise de Variância , Citocinas/análise , Polpa Dentária/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Citometria de Fluxo
7.
Braz. j. med. biol. res ; 50(7): e5782, 2017. graf
Artigo em Inglês | LILACS | ID: biblio-951699

RESUMO

Endometriosis is a benign, estrogen-dependent disease with symptoms such as pelvic pain and infertility, and it is characterized by the ectopic distribution of endometrial tissue. The expression of the ID2, PRELP and SMOC2 genes was compared between the endometrium of women without endometriosis in the proliferative phase of their menstrual cycle and the eutopic and ectopic endometrium of women with endometriosis in the proliferative phase. Paired tissue samples from 20 women were analyzed: 10 from endometrial and peritoneal endometriotic lesions and 10 from endometrial and ovarian endometriotic lesions. As controls, 16 endometrium samples were collected from women without endometriosis in the proliferative phase of menstrual cycle. Analysis was performed by real-time polymerase chain reaction (PCR). There was no significant difference between gene expression in the endometrium of women with and without endometriosis. The ID2 gene expression was increased in the most advanced stage of endometriosis and in ovarian endometriomas, the PRELP was more expressed in peritoneal lesions, and the SMOC2 was highly expressed in both peritoneal and endometrioma lesions. Considering that the genes studied participate either directly or indirectly in cellular processes that can lead to cell migration, angiogenesis, and inappropriate invasion, it is possible that the deregulation of these genes caused the development and maintenance of ectopic tissue.


Assuntos
Humanos , Feminino , Adolescente , Adulto , Adulto Jovem , Doenças Peritoneais/genética , Glicoproteínas/genética , Osteonectina/genética , Proteínas da Matriz Extracelular/genética , Endometriose/genética , Proteína 2 Inibidora de Diferenciação/genética , Glicoproteínas/metabolismo , Estudos de Casos e Controles , Regulação da Expressão Gênica , Proteínas da Matriz Extracelular/metabolismo , Endometriose/metabolismo , Proteína 2 Inibidora de Diferenciação/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Ciclo Menstrual
8.
Int. j. morphol ; 34(2): 763-769, June 2016. ilus
Artigo em Inglês | LILACS | ID: lil-787066

RESUMO

The aim of this study was to evaluate the effects of melatonin healing in a tibial bone defect model in rats by means of histopathological and immunohistochemistry analysis. Twenty one male Wistar albino rats were used in this study. In each animal, bone defects (6 mm length ) were created in the tibias. The animals were divided into three groups. In group 1 control group (rats which tibial defects). Group 2 melatonin (10 mg/kg) + 14 days in the tibial defect group) was administered intraperitoneally to rats. Group 3 melatonin (10 mg/kg) + 28 days in the tibial defect group) was administered intraperitoneally to rats. Histopathological analysis of samples was performed to evaluate the process of osteoblastic activity, matrix formation, trabecular bone formation and myeloid tissue in bone defects. Immunohistochemical and immunoblot analysis demonstrated non-collagenous proteins (osteopontin and osteonectin) differences in tibial bone defects. The expression of osteopontin on tibia was increased by 14 days melatonin treatment. The expression of osteonectin on tibia was dramatically increased by 14 days melatonin treatment.


El objetivo fue evaluar por medio de análisis histopatológico e inmunohistoquímico los efectos cicatrizantes de la melatonina en un modelo de defecto óseo tibial en ratas. Se utilizaron 21 ratas albinas Wistar macho. En cada animal, se crearon defectos óseos en las tibias de 6 mm de longitud. Los animales se dividieron en tres grupos. El Grupo 1 correspondió al grupo control (defectos tibiales sin tratamiento). Al Grupo 2 se administró melatonina por vía intraperitoneal (10 mg/kg) 14 días posteriores al defecto tibial. Al Grupo 3 se administró melatonina por vía intraperitoneal (10 mg/kg) 28 días posteriores al defecto tibial. Se realizó un análisis histopatológico para evaluar los procesos de actividad osteoblástica, formación de matriz, formación de hueso trabecular y tejido mieloide en los defectos óseos. Los análisis inmunohistoquímicos y de inmunotransferencia mostraron diferencias de proteínas no colágenas (osteopontina y osteonectina). La expresión de osteopontina en defectos óseos tibiales se incrementó en el Grupo 2. La expresión de osteonectina en la tibia se incrementó fuertemente bajo el tratamiento con melatonina por 14 días.


Assuntos
Animais , Ratos , Melatonina/farmacologia , Fraturas da Tíbia/tratamento farmacológico , Tíbia/efeitos dos fármacos , Modelos Animais de Doenças , Melatonina/administração & dosagem , Osteonectina/efeitos dos fármacos , Osteonectina/metabolismo , Osteopontina/efeitos dos fármacos , Osteopontina/metabolismo , Ratos Sprague-Dawley , Fraturas da Tíbia/patologia , Tíbia/patologia , Cicatrização/efeitos dos fármacos
9.
Braz. oral res. (Online) ; 30(1): e93, 2016. graf
Artigo em Inglês | LILACS | ID: biblio-952019

RESUMO

Abstract The aim of the present study was to evaluate the expression of transforming growth factor-β1 (TGF-β1) and osteonectin (ON) in pulp-like tissues developed by tissue engineering and to compare it with the expression of these proteins in pulps treated with Ca(OH)2 therapy. Tooth slices were obtained from non-carious human third molars under sterile procedures. The residual periodontal and pulp soft tissues were removed. Empty pulp spaces of the tooth slice were filled with sodium chloride particles (250-425 µm). PLLA solubilized in 5% chloroform was applied over the salt particles. The tooth slice/scaffold (TS/S) set was stored overnight and then rinsed thoroughly to wash out the salt. Scaffolds were previously sterilized with ethanol (100-70°) and washed with phosphate-buffered saline (PBS). TS/S was treated with 10% EDTA and seeded with dental pulp stem cells (DPSC). Then, TS/S was implanted into the dorsum of immunodeficient mice for 28 days. Human third molars previously treated with Ca(OH)2 for 90 days were also evaluated. Samples were prepared and submitted to histological and immunohistochemical (with anti-TGF-β1, 1:100 and anti-ON, 1:350) analyses. After 28 days, TS/S showed morphological characteristics similar to those observed in dental pulp treated with Ca(OH)2. Ca(OH)2-treated pulps showed the usual repaired pulp characteristics. In TS/S, newly formed tissues and pre-dentin was colored, which elucidated the expression of TGF-β1 and ON. Immunohistochemistry staining of Ca(OH)2-treated pulps showed the same expression patterns. The extracellular matrix displayed a fibrillar pattern under both conditions. Regenerative events in the pulp seem to follow a similar pattern of TGF-β1 and ON expression as the repair processes.


Assuntos
Humanos , Animais , Camundongos , Células-Tronco/efeitos dos fármacos , Hidróxido de Cálcio/farmacologia , Osteonectina/análise , Polpa Dentária/efeitos dos fármacos , Fator de Crescimento Transformador beta1/análise , Fatores de Tempo , Hidróxido de Cálcio/uso terapêutico , Imuno-Histoquímica , Osteonectina/efeitos dos fármacos , Células Cultivadas , Reprodutibilidade dos Testes , Engenharia Tecidual/métodos , Polpa Dentária/citologia , Dentina/efeitos dos fármacos , Regeneração Tecidual Guiada/métodos , Matriz Extracelular/efeitos dos fármacos , Fator de Crescimento Transformador beta1/efeitos dos fármacos , Tecidos Suporte , Odontoblastos/efeitos dos fármacos
10.
J. appl. oral sci ; 22(6): 541-553, Nov-Dec/2014. graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: lil-732593

RESUMO

Objective The aim of this paper was to evaluate the repair of onlay autogenous bone grafts covered or not covered by an expanded polytetrafluoroethylene (e-PTFE) membrane using immunohistochemistry in rats with induced estrogen deficiency. Material and Methods Eighty female rats were randomly divided into two groups: ovariectomized (OVX) and with a simulation of the surgical procedure (SHAM). Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM). Animals were euthanized on days 0, 7, 21, 45, and 60. The specimens were subjected to immunohistochemistry for bone sialoprotein (BSP), osteonectin (ONC), and osteocalcin (OCC). Results All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense. At the 45-day, the bone graft showed direct bonding to the recipient bed in all specimens. The ONC and OCC showed more expressed in granulation tissue, in the membrane groups, independently of estrogen deficiency. Conclusions The expression of bone forming markers was not negatively influenced by estrogen deficiency. However, the markers could be influenced by the presence of the e-PTFE membrane. .


Assuntos
Animais , Feminino , Regeneração Óssea/fisiologia , Transplante Ósseo/métodos , Regeneração Tecidual Guiada/métodos , Politetrafluoretileno/uso terapêutico , Biomarcadores/análise , Estrogênios/deficiência , Imuno-Histoquímica , Sialoproteína de Ligação à Integrina/análise , Mandíbula/cirurgia , Osteoblastos/fisiologia , Osteocalcina/análise , Osteonectina/análise , Osteoporose/fisiopatologia , Ovariectomia , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes , Fatores de Tempo , Resultado do Tratamento
11.
RPG, Rev. Pós-Grad ; 16(4): 179-187, out.-dez. 2009. tab, ilus
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-855246

RESUMO

A aplicação de ozônio pode ser usada como uma alternativa no tratamento de inúmeras patologias. O objetivo é interferir positivamente na reparação tecidual e agir como anti-séptico, uma vez que o ozônio é um potente agente antimicrobiano e possui a capacidade de estimular a circulação sanguínea e a resposta imunomodulatória. Tais características justificam o interesse da sua aplicação na Medicina e na Odontologia. Quando em contato com fluidos orgânicos, o ozônio age como um oxidante gerando a formação de moléculas reativas do oxigênio e produtos de lipídeos oxidantes que influenciam em eventos bioquímicos do metabolismo celular. A proposição deste estudo foi avaliar a aplicação do ozônio diluído em água no processo de reparação óssea por meio de análise morfológica e imunoistoquímica em modelo animal. Os resultados mostraram que a irrigação da ferida cirúrgica com ozônio diluído em água ocasionou atraso na reparação e que esse era mais exuberante no início do processo


Assuntos
Animais , Masculino , Ratos , Matriz Óssea , Cicatrização , Ozônio , Durapatita , Osteoclastos , Osteonectina , Osteopontina
12.
São Paulo; s.n; 2010. 85 p. ilus, tab.
Tese em Português | LILACS | ID: lil-620019

RESUMO

INTRODUÇÃO: O câncer de mama é uma das mais importantes causas de mortalidade feminina no mundo. Acredita-se que as lesões proliferativas do parênquima mamário sejam marcadoras de risco para câncer ou precursoras do carcinoma mamário. Apesar da intensa pesquisa na área do câncer de mama, os eventos moleculares precoces associados à evolução e progressão do câncer mamário ainda são pouco conhecidos. OBJETIVOS: Com a expectativa de melhor compreender os eventos iniciais da carcinogênese mamária examinamos um conjunto de oitenta e quarto lesões proliferativas mamárias quanto à expressão dos genes N-myc down regulated gene (NDRG1), Prostate Apoptosis Response-4 (PAR-4), Osteonectina e Pontina. A expressão destes genes foi documentada, por trabalhos prévios em nosso laboratório ou por estudos de outros autores, como tendo impacto na evolução do câncer de mama. MÉTODOS: Construímos um TMA com lesões proliferativas da mama e testamos este TMA por método imunohistoquímico para Ndrg1, Par-4, Osteonectina e Pontina, bem como para o receptor de estrógeno e citoqueratinas de alto e baixo peso molecular, com o objetivo de caracterizar as lesões presentes no TMA. A avaliação imunohistoquímica foi feita de forma quantitativa, com o aplicativo e sistema de análise quantitativa para TMA ACIS III, da Dako. RESULTADOS: Após excluirmos amostras não informativas, contamos com 68 amostras de lesões proliferativas mamárias para análise. Nestas, observamos uma notável positividade de NDRG1, em lesões com morfologia apócrina. Observamos ainda alta expressão de NDRG1 em lesões proliferativas mamárias, quando comparadas aos outros tipos de lesões presentes no TMA. Pontina exibiu os mais altos valores de expressão nos casos de lesões proliferativas mamárias, com valor de p estatisticamente significativo. A expressão de PAR-4 foi predominantemente nuclear nas lesões mamárias analisadas no TMA. Osteonectina teve expressão diferenciada...


INTRODUCTION: Breast cancer is a leading cause of death among women all over the Word. Proliferative lesions of the breast are believed to be precursors of or markers of increased risk for breast carcinoma. Although the active research in the field of breast cancer, the early molecular events associated with cancer evolution and progression are still poorly understood. OBJECTIVES: In order to better understand the early events in the breast carcinogenesis, we examined a set of eighty-four proliferative lesions of the breast for the expression of N-myc down regulated gene (NDRG1), Prostate Apoptosis Response-4 (PAR-4), Osteonectin and Pontin, which expression was previously shown by our laboratory to have impact in breast cancer prognosis. METHODS: A tissue microarray were constructed and immunohistochemically tested for Ndrg1, Par-4, Osteonectin and Pontin together with estrogen receptor, low and high weight cytokeratins, aiming to properly characterize the lesions sorted in the tissue microarray. Immunohistochemistry assessment was made quantitative, with the ACIS III Dako quantitative analysis system and TMA application software. RESULTS: After excluding non informative cores, cores with fibroadipose tissue or mammary parenchyma with normal appearing breast epithelium, we ended up with a TMA with 68 breast lesions. Of those, we observed a noticeable positivity of Ndrg1 for lesions with apocrine morphology. Additionally, all cases of florid epithelial hyperplasia showed variable high immunoexpression levels of protein, when compared to the other sorted out lesions in the TMA. Pontin expression level was highest among the breast hyperplasia cases, with statistically significant p value. PAR-4 protein expression was found to be predominantly localized in the nucleous in the non-malignant breast lesions analyzed. Osteonectin exhibited differentiated expression values in the epithelium of hyperplastic breast lesions, papillomas and multiple papillomas when...


Assuntos
Humanos , Feminino , Doenças Mamárias , Neoplasias da Mama , Expressão Gênica , Osteonectina
13.
Biocell ; 33(1): 39-47, Apr. 2009. ilus
Artigo em Inglês | LILACS | ID: lil-595028

RESUMO

Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cel ls, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo.


Assuntos
Masculino , Animais , Camundongos , Colágeno Tipo I/biossíntese , Colágeno Tipo I/genética , Odontoblastos/citologia , Odontoblastos/metabolismo , Regeneração Óssea/fisiologia , Regeneração Óssea/genética , Sialoglicoproteínas/biossíntese , Sialoglicoproteínas/genética , Osso e Ossos/citologia , Osso e Ossos/metabolismo , Camundongos SCID , Osteocalcina/biossíntese , Osteocalcina/genética , Osteonectina/biossíntese , Osteonectina/genética , Osteopontina/biossíntese , Osteopontina/genética
14.
Periodontia ; 19(3): 117-126, 2009. ilus, tab
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-587920

RESUMO

O estudo da reparação periodontal deve se basear em modelos que forneçam os dados necessários para a análise deste fenômeno. O modelo proposto, de defeitos agudos de furca classe II, parece ser o mais indicado, pois possui um grau previsível de regeneração espontânea, fornecendo um ambiente em que é possível o estudo dos fatores que favorecem a regeneração dos tecidos periodontais. O objetivo deste trabalho foi avaliar a qualidade da reparação de defeitos agudos de furca classe II por meio da imunoexpressão de proteínas da matriz extracelular (MEC). Após a elevação de retalhos de espessura total, em quatro cães, foram criados defeitos agudos de furca classe II nos segundos, terceiros e quartos pré-molares de um dos lados da mandíbula. Os retalhos foram posicionados coronariamente e suturados. Após 45 dias, os espécimes foram coletados, descalcificados e analisados, em um plano vestíbulo- lingual, por meio de técnica imunohistoquímica para osteopontina (OPN), sialoproteína óssea (BSP) e osteonectina (BON). Nos tecidos periodontais originais, a marcação para os anticorpos testados foi fracamente positiva na MEC, caracterizando a presença de tecidos maduros. Já no interior dos defeitos, houve diferença na marcação, com marcação mais pronunciada para BSP, OPN e BON. Conclui-se que os resultados evidenciam maior atividade metabólica nos tecidos reparativos.


The study of periodontal repair should be based on models that provide the data required for analysis of thisphenomenon. The proposed model of acute class II furcation defects seems to be the most appropriate, as it provides apredictable degree of spontaneous regeneration, creating an environment where the factors that promote the regeneration of periodontal tissues can be studied. The objective of this study was to evaluate the quality of repair ofacute class II furcation defects, by means of immunoexpression of extracellular matrix (ECM) proteins.After lifting full thickness flaps in 4 dogs, acute class II furcation defects were created in the second, third and fourth premolar in one side of the mandible. The flaps were coronallypositioned and sutured. After 45 days, the specimens were collected, decalcified and analyzed in a buccal-lingual plane, by immunohistochemistry technique for osteopontin (OPN), bone sialoprotein (BSP) and osteonectin (BON). In original periodontal tissues, the marking for the tested antibodieswas weakly positive in the ECM, characterizing the presence of mature tissues. Inside the defects, there was a difference in the marking, with markings more pronounced for BSP, OPN and BON. It was concluded that the results showed greater metabolic activity in the reparative tissues.


Assuntos
Animais , Cães , Regeneração Óssea , Osteonectina , Osteopontina , Ligamento Periodontal , Sialoglicoproteínas
15.
In. Douglas, Carlos Roberto. Patofisiologia oral: fisiologia normal e patológica aplicada a odontologia e fonoaudiologia. Säo Paulo, Pancast, 1998. p.397-403, ilus. (BR).
Monografia em Português | LILACS, BBO - Odontologia | ID: lil-246800
16.
RPG rev. pos-grad ; 3(3): 220-29, jul.-set. 1996. ilus, graf
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-197599

RESUMO

Apresentamos metodologia para obtençäo de linhagem de células osteoblástica propagada a partir de tecido ósseo normal. Por meio de técnica de dispersäo enzimática células isoladas do osso parietal de ratos recém-nascidos foram cultivadas em meio de Eagle modificado por Dullbecco supplementado com soro fetal bovino (10 por cento). Após a primeira passagem as culturas foram induzidas pela suplementaçäo do meio com ß-glicerofosfato de sódio (10mM), ácido ascórbico (50ug/ml) e dexametasona (10 elevado a -8 M) para obtençäo de diferenciçäo fenotípica e funcional. A morfologia, presença de atividade de fosfatase alcalina, produçäo de nódulos calcificados, expressäo imunocitoquímica de proteínas do tecido ósseo colagênicas (colágeno tipo I) e näo colagênicas (osteonectina e sialoproteína óssea-II) comprovaram a natureza osteoblástica da linhagem obtida. O sistema desenvolvido confirmou que a técnica de cultivo celular primário a partir de tecido ósseo normal é viável e origina células com as características básicas dos osteoblastos. Essa metodologia de obtençäo de células osteoblásticas pode ser utilizada amplamente em Odontologia, em especial, nos estudos da fisio-patologia óssea e em testes de compatibilidade de biomateriais que estaräo em contato constante com o tecido ósseo


Assuntos
Animais , Ratos , Células Cultivadas/citologia , Colágeno , Osteoblastos/citologia , Osteonectina/fisiologia , Sialoglicoproteínas/análise
17.
Medicina (B.Aires) ; 56(1): 51-4, ene.-feb. 1996. ilus, tab
Artigo em Espanhol | LILACS | ID: lil-163385

RESUMO

En un estudio previo demostramos que líneas celulares y tumores de melanoma humano expresan altos niveles de la proteína de matriz extracelular SPARC. Para determinar su rol en la progresión del melanoma humano, la línea IIB-MELLES fue transfectada con el cDNA de SPARC anti-sentido. Se aislaron tres clones con expresión disminuida de SPARC. Ninguno de ellos mostró cambios en la cinética de crecimiento in vitro comparado con las células control. La inyección s.c. de células control en ratones atímicos mostró desarrollo tumoral en el 100 por ciento de los animales, mientras que ninguno de los clones dio origen a tumores. Estos estudios demuestran que SPARC podría jugar un rol central en la progresión del melanoma humano.


Assuntos
Animais , Masculino , Camundongos , Humanos , Melanoma/patologia , Osteonectina/fisiologia , Northern Blotting , Western Blotting , Células Clonais , DNA Antissenso/genética , Melanoma/metabolismo , Camundongos Endogâmicos BALB C , Osteonectina/metabolismo , Ratos Nus , Fatores de Tempo , Células Tumorais Cultivadas
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...