Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros










Filtros aplicados
Intervalo de ano de publicação
1.
J. appl. oral sci ; 24(6): 597-606, Nov.-Dec. 2016. graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-841153

RESUMO

ABSTRACT Objectives The aim of this study was to explore the effect of capsaicin on expression patterns of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) and trigeminal subnucleus caudalis (Vc) following experimental tooth movement. Material and Methods Male Sprague-Dawley rats were used in this study and divided into small-dose capsaicin+force group, large-dose capsaicin+force group, saline+force group, and no force group. Closed coil springs were used to mimic orthodontic forces in all groups except for the no force group, in which springs were inactivated. Capsaicin and saline were injected into periodontal tissues. Rats were euthanized at 0 h, 12 h, 1 d, 3 d, 5 d, and 7 d following experimental tooth movement. Then, TG and Vc were obtained for immunohistochemical staining and western blotting against CGRP. Results Immunohistochemical results indicated that CGRP positive neurons were located in the TG, and CGRP immunoreactive fibers were distributed in the Vc. Immunohistochemical semiquantitative analysis and western blotting analysis demonstrated that CGRP expression levels both in TG and Vc were elevated at 12 h, 1 d, 3 d, 5 d, and 7 d in the saline + force group. However, both small-dose and large-dose capsaicin could decrease CGRP expression in TG and Vc at 1 d and 3 d following experimental tooth movement, as compared with the saline + force group. Conclusions These results suggest that capsaicin could regulate CGRP expression in TG and Vc following experimental tooth movement in rats.


Assuntos
Animais , Masculino , Técnicas de Movimentação Dentária/métodos , Núcleo Inferior Caudal do Nervo Trigêmeo/efeitos dos fármacos , Capsaicina/farmacologia , Peptídeo Relacionado com Gene de Calcitonina/efeitos dos fármacos , Gânglio Trigeminal/efeitos dos fármacos , Fármacos do Sistema Sensorial/farmacologia , Valores de Referência , Fatores de Tempo , Núcleo Inferior Caudal do Nervo Trigêmeo/química , Dor Facial , Imuno-Histoquímica , Cloreto de Sódio , Distribuição Aleatória , Peptídeo Relacionado com Gene de Calcitonina/análise , Western Blotting , Gânglio Trigeminal/química , Reprodutibilidade dos Testes , Ratos Sprague-Dawley
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...