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1.
Pesqui. bras. odontopediatria clín. integr ; 23: e210162, 2023. tab, graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1431047

RESUMO

ABSTRACT Objective: To compare the agreement of images in white light (WL), fluorescence (FL), and digital radiographs (DR), on the diagnosis and treatment decisions for occlusal caries lesions against a micro-CT gold standard. Material and Methods: Ten extracted third molars, with enamel and/or dentin caries (ICDAS 2-4), were included. Occlusal surface images were acquired with an intraoral camera (SoproLife®) in WL and FL modes. DR was obtained using an intraoral X-ray and a semi-direct digital system. A total of 780 images were needed, organized in a template, to be later examined by twenty-six dentists invited to compose the study. The Generalized Estimation Equations model was used to compare the proportions of the correct answers between the three methods and the gold standard. When significant, Bonferroni post-hoc test was used to identify differences (α=5%). Results: Most of the examiners were specialists (76.9%) with 14.5 years of experience. All diagnostic methods were similar and showed low agreement (DR 12.7%, WL 16.5%, and FL 16.5%) compared with gold standard caries diagnostic scores. Regarding treatment decisions, mean agreement for all diagnostic methods was higher (43.2%; p<0.001), and among all methods, WL (48.1%) and FL (51.2%) modes performed better than DR (30.4%, p<0.001). Conclusion: SoproLife® images could help clinicians to propose rational, minimally invasive treatments for occlusal caries lesions.


Assuntos
Humanos , Cárie Dentária/diagnóstico por imagem , Microtomografia por Raio-X/métodos , Fluorescência , Tomada de Decisão Clínica , Dente Serotino/lesões , Efetividade , Técnicas In Vitro/métodos , Estudos Transversais/métodos , Radiografia Dentária Digital/métodos
2.
São Paulo; s.n; s.n; 2023. 125 p. tab, graf, ilus.
Tese em Português | LILACS | ID: biblio-1437845

RESUMO

O dano capilar causado pelo descolorimento oxidativo é muito intenso, sendo que dois fatores são responsáveis por essa ação: primeiro, a ação direta e danosa do oxidante em diversas estruturas capilares e segundo, o dano oxidativo primário facilita o dano causado por outros agentes físicos (luz, temperatura) e químicos (tensoativos), que comumente tem ação nos cabelos. Desenvolver conceitos e tecnologias que possam tornar o oxidante específico para a melanina e por conseguinte efetuando o descolorimento sem causar danos ao fio é extremamente desejável. Neste trabalho buscaremos entender de que forma a luz visível pode aumentar a ação do oxidante sem danificar o fio colateralmente. O objetivo principal deste trabalho é demonstrar que é possível utilizar a luz visível, que é absorvida pela melanina, para tornar esse pigmento mais suscetível ao agente oxidante e desta forma, permitir que o descolorimento seja realizado com concentrações pequenas de oxidante. Também almejamos desenvolver métodos de análises por microscopia ótica de fluorescência e de reflexão para mensurar o dano nas estruturas dos fios processados com oxidante e na presença ou ausência da luz


The capillary damage caused by oxidative discoloration is very intense, and two factors are responsible for this action: first, the direct and harmful action of the oxidant on several capillary structures and second, the primary oxidative damage facilitates the damage caused by other physical agents (light, temperature) and chemicals (surfactants), which commonly have action on the hair. Developing concepts and technologies that can make the oxidant specific to melanin and therefore discoloring without causing damage to the hair is extremely desirable. In this work we will try to understand how visible light can increase the oxidant's action without damaging the wire collaterally. The main objective of this work is to demonstrate that it is possible to use visible light, which is absorbed by melanin, to make this pigment more susceptible to the oxidizing agent and, thus, to allow the discoloration to be carried out with small concentrations of oxidizer. We also aim to develop methods of analysis by optical fluorescence and reflection microscopy to measure the damage to the structures of the threads processed with oxidizer and in the presence or absence of light


Assuntos
Oxidação , Descolorantes de Cabelo/efeitos adversos , Luz/efeitos adversos , Melaninas/agonistas , Compostos Químicos , Fluorescência , Cabelo , Microscopia/métodos
3.
Rev. cir. (Impr.) ; 74(4): 426-431, ago. 2022. ilus
Artigo em Espanhol | LILACS | ID: biblio-1407931

RESUMO

Resumen El verde de indocianina es un tinte que se ha utilizado en medicina durante varias décadas. Tiene una serie de aplicaciones, incluida la cirugía reconstructiva y las quemaduras. Permite detectar áreas de tejido con perfusión reducida, lo que reduce el riesgo de complicaciones posoperatorias en forma de procesos de cicatrización alterados y necrosis. La técnica de imágenes que utiliza este tinte, permite observar los cambios en la fluorescencia en tiempo real y que, se ha demostrado, ocurren entre las capas superficiales y profundas en las quemaduras. Esto permite un diagnóstico cualitativo y cuantitativo de la profundidad de la quemadura, lo que se traduce en la elección de un tratamiento adicional. Se aprecia la importancia particular de este método en la prevención de la necrosis cutánea con el complejo areola-pezón durante la reconstrucción mamaria simultánea. Se necesitan más ensayos controlados aleatorios prospectivos para considerarlo el "método de elección" en la práctica clínica.


Indocyanine green is a dye that has been used in medicine for several decades. It has a number of applications, including reconstructive surgery and burns. It allows the detection of areas of tissue with reduced perfusion, which reduces the risk of postoperative complications in the form of altered healing processes and necrosis. The imaging technique that uses this dye allows us to observe the changes in fluorescence in real time that have been shown to occur between the superficial and deep layers in burns. This allows a qualitative and quantitative diagnosis of the depth of the burn, which results in the choice of additional treatment. The particular importance of this method in the prevention of skin necrosis with the areolanipple complex is appreciate during simultaneous breast reconstruction. More prospective randomized controlled trials are needed to consider it the 'method of choice' in clinical practice.


Assuntos
Humanos , Queimaduras/diagnóstico , Corantes/uso terapêutico , Verde de Indocianina/uso terapêutico , Cicatrização , Fluorescência , Mastectomia
4.
Rev. argent. cir ; 114(1): 20-25, mar. 2022. graf
Artigo em Inglês, Espanhol | LILACS, BINACIS | ID: biblio-1376372

RESUMO

RESUMEN Antecedentes: en la última década ha comenzado a investigarse el uso de la captación de fluorescencia mediante luz infrarroja para la visualización de ganglios linfáticos en tumores de estómago y esófago. Objetivo: evaluar la factibilidad de la evaluación del drenaje linfático de cáncer de esófago mediante el uso de fluorescencia y verde de indocianina (ICG). Material y métodos: se realizó un estudio prospectivo que incluyó pacientes con tumores de la unión gastroesofágica resecables (estadios I, II y III). Antes de comenzar la cirugía se inyectaron por endoscopia 4 mL de ICG doblemente diluida en agua estéril (1,25 mg/mL = 5 mg) en la submucosa del esófago en los cuatro cuadrantes (1 mL por cuadrante) alrededor del tumor. Resultados: se incluyeron en total 6 pacientes. En todos ellos se logró identificar el drenaje linfático del tumor hacia la primera estación ganglionar: en 6/6 (100%), el drenaje linfático con fluorescencia se detectó en las estaciones ganglionares N°s 3 y 7 (curvatura menor y arteria gástrica izquierda). En ningún paciente se identificó fluorescencia en ganglios mediastinales. Conclusión: la visualización del drenaje linfático de tumores de la unión gastroesofágica mediante el uso de fluorescencia con ICG es factible.


ABSTRACT Background: Over the past decade, fluorescence imaging with infrared light has been used to visualize lymph nodes in tumors of the stomach and esophagus. Objective: The aim of our study was to evaluate the feasibility of evaluating lymphatic drainage in esophageal cancer using fluorescence and indocyanine green (ICG). Material and methods: We conducted a prospective study of patients with resectable tumors of the gastroesophageal junction (stage I, II and III). Before surgery, 4 mL of ICG double diluted in sterile water (1.25 mg/mL = 5 mg) were injected via endoscopy into the esophageal submucosa in the four quadrants (1 mL per quadrant) around the tumor. Results: A total of 6 patients were included. Lymphatic drainage from the tumor to the first lymph node station was identified in all patients: in 6/6 (100%), fluorescent lymphatic drainage was detected in nodal stations number 3 and 7 (lesser curvature and left gastric artery) Fluorescence was not identified in the mediastinal lymph nodes in any patient. Conclusion: Visualization of lymphatic drainage of gastroesophageal junction tumors to the lesser curvature nodes using fluorescence imaging is feasible.


Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Idoso , Neoplasias Gástricas , Neoplasias Esofágicas/cirurgia , Esofagectomia , Argentina , Estudos de Viabilidade , Estudos Prospectivos , Junção Esofagogástrica , Fluorescência , Artéria Gástrica , Verde de Indocianina , Excisão de Linfonodo
5.
Biosci. j. (Online) ; 38: e38066, Jan.-Dec. 2022. ilus, graf, tab
Artigo em Inglês | LILACS | ID: biblio-1396895

RESUMO

A wide range of soybean cultivars is available on the market and understanding the physiological response and yield of these materials is fundamental to develop new management systems. Thus, the objective of the present study was to assess ecophysiological parameters and yield of soybean cultivars under field conditions. The experiment was carried out on a farm located in the municipality of Açailândia, Maranhão, Brazil. Three commercial cultivars were used (SC1, SC2 and SC3), and gas exchanges, SPAD index, Fv/Fm, photosynthesis index (PI), instantaneous water use efficiency (WUE) and intrinsic instantaneous of the use of water (iWUE) were assessed during the vegetative (V5) and reproductive (R5) stages. In addition, the biomass and production components were obtained. A randomized complete block design was used, with three cultivars and six replications. SC2 obtained the best mean for the photochemical variables. SC2 was more efficient at both development stages in WUE, but the maximum iWUE values were obtained in SC3. The SC2 cultivar obtained the best responses in the main variables analyzed, resulting in a higher yield.


Assuntos
Soja , Clorofila , Uso Eficiente da Água , Fluorescência
6.
Arq. odontol ; 58: 63-86, 2022. tab, graf, ilus
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-1380554

RESUMO

Objetivo: O objetivo dessa revisão sistemática e meta-análise foi avaliar a acurácia de dispositivos de indução de fluorescência e do método radiográfico para diagnóstico da cárie dentária em dentes decíduos. Métodos: Buscas no PubMed, Web of Science, Scopus, Embase, Open Grey e Google Scholar foram realizadas em agosto de 2021. Referências que preenchiam os critérios de elegibilidade foram incluídas. O risco de viés dos artigos incluídos foi avaliado com a ferramenta QUADAS-2. Foram realizadas meta-analises com o MedCalc. Resultados fornecidos em área sob a curva (ASC), intervalo de confiança (IC) e erro padrão (EP). Resultados: Títulos/resumos de 619 referências foram avaliados por dois revisores calibrados de forma independente. Cinquenta e seis artigos foram selecionados para leitura do texto completo. Onze artigos foram incluídos na revisão-sistemática e meta-análise. Os artigos incluídos apresentaram baixo risco de viés. A ASC para diagnóstico de cárie em esmalte com o laser DIAGNOdent foi de 75,8% (IC = 68,1% - 83,6% / EP = 3,9% / I2 = 67,95%) e para o diagnóstico de cárie em dentina foi de 80,6% (ASC = 80,6% / IC = 65,7% - 95,6% / EP = 7,6% / I2 = 89,24%). A ASC para o diagnóstico de cárie em esmalte com a radiografia interproximal foi de 61,7% (IC = 51,4% - 72,1% / EP = 5,3% / I2 = 44,51%) e a ASC para diagnóstico de cárie em dentina com a radiografia interproximal foi de 73,7% (IC = 45,1% - 100% / EP = 14,6% / I2 = 96,97%). Conclusão: O método radiográfico e os dispositivos de indução de fluorescência são boas opções para diagnóstico de cárie em dentes decíduos. Ambos apresentam melhores resultados em lesões em dentina. Os métodos que utilizam fluorescência tiveram melhores resultados em esmalte quando comparados à radiografia.


Aim: This systematic review and meta-analysis aimed to evaluate the accuracy of the fluorescence induction devices and radiographic method for diagnosing dental caries in primary teeth. Methods: Searches in PubMed, Web of Science, Scopus, Embase, Open Grey, and Google Scholar were conducted in August 2021. References that met the eligibility criteria were included. The risk of bias of the included articles was assessed with QUADAS-2. Meta-analyses were performed with MedCalc. Results were provided as area under the curve (AUC), confidence interval (CI), and standard error (SE). Results: Titles/abstracts of 619 references were evaluated by two trained reviewers independently. Fifty-six articles were selected for full-text reading. Eleven articles were included in the systematic review and meta-analysis. The included articles showed a low risk of bias. The AUC for the diagnosis of caries on enamel with the DIAGNOdent laser was 75.8% (CI = 68.1% - 83.6% / SE = 3.9% / I2 = 67.95%), whereas for the diagnosis of caries on dentin, it was 80.6% (AUC = 80.6% / CI = 65.7% - 95.6% / SE = 7.6% / I2 = 89.24%). The AUC for the diagnosis of enamel caries with the interproximal radiograph was 61.7% (CI = 51.4% - 72.1% / SE = 5.3% / I2 = 44.51%) and the AUC for the diagnosis of dentin caries with the interproximal radiograph was 73.7% (CI = 45.1% - 100% / SE = 14.6% / I2 = 96.97%). Conclusion: Radiography and fluorescence devices are useful tools for the diagnosis of caries in deciduous teeth. Both showed better results in dentin lesions. The methods using fluorescence had better results in enamel when compared to radiography.


Assuntos
Radiografia , Cárie Dentária , Fluorescência , Revisão Sistemática
7.
Braz. J. Pharm. Sci. (Online) ; 58: e18674, 2022. graf
Artigo em Inglês | LILACS | ID: biblio-1374556

RESUMO

Abstract Fluoride anions are indispensable trace elements required for sustaining life. To investigate the homeostasis and action of fluoride in the body, a new highly sensitive and selective fluorescence detection method was designed for fluoride in aqueous solutions. A fluorescent probe for fluoride (FP-F) was synthesized for imaging F- in living cells. The design strategy for the probe was based on the specific reaction between fluoride and silica to mediate deprotection of this probe to fluorescein. Upon treatment with F-, FP-F, a closed and weakly fluorescent lactone, was transformed into an open and strongly fluorescent product. Under the optimum conditions, the detection limit for fluoride was 0.526 nM. FP-F could detect micromolar changes in F- concentrations in living cells by confocal microscopy.


Assuntos
Fluoresceína/farmacologia , Fluorescência , Flúor/análise , Oligoelementos/efeitos adversos , Células/metabolismo , Microscopia Confocal/métodos , Diagnóstico , Corantes Fluorescentes/farmacologia , Homeostase , Métodos
8.
Braz. J. Pharm. Sci. (Online) ; 58: e19692, 2022. graf
Artigo em Inglês | LILACS | ID: biblio-1384014

RESUMO

Abstract The development of stable cell lines producing recombinant proteins is very time-consuming and laborious. One of the practical approaches successfully performed is Fluorescence-Activated Cell Sorting (FACS). A mutated chimeric tissue plasminogen activator (mt-PA) was developed by removing the first three domains of t-PA, insertion of GHRP sequence and mutation toward resistance to plasminogen activator inhibitor-1 (PAI-1). In the current study, a new stable CHO-DG44 cell line producing mt-PA was developed by two sequential clonal selections: FACS and clonal-selection by limiting dilution. Furthermore, the expression was more evaluated using two different expression media. Finally, the high-producing clones were selected based on the dot blot and amidolytic activity test. The transfection efficiency of CHO-DG44 cells was 38% as measured by flow cytometry on green fluorescent protein (GFP). After performing FACS on stable cell pools, the expression yield was increased to fifty-fold. In terms of growth profile, CD-DG44 showed higher viability and cell density results than ProCHO5 medium. The expression of mt-PA was significantly higher in CD-DG44 than in ProCHO5, 765 and 280 IU/mL, respectively. Our data indicated that selection of an appropriate expression medium played a critical role in the development of potent producing stable cells by FACS.


Assuntos
Ativador de Plasminogênio Tecidual , Otimização de Processos , Citometria de Fluxo/métodos , Fluorescência , Contagem de Células/instrumentação , Células Clonais/classificação , Inibidor 1 de Ativador de Plasminogênio/efeitos adversos , Proteínas de Fluorescência Verde
9.
Int. j. morphol ; 39(6): 1731-1736, dic. 2021. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1385528

RESUMO

SUMMARY: Regeneration of the dura mater following duraplasty using a collagen film, a chitosan film, or a combination of both with gelatin, was studied in a craniotomy and penetrating brain injury model in rats. Collagen autofluorescence in the regenerated dura mater was evaluated using confocal microscopy with excitation at λem = 488 nm and λem = 543 nm. An increase in regeneration of the extracellular matrix of connective tissue and an increase in matrix fluorescence were detected at 6 weeks after duraplasty. The major contributors to dura mater regeneration were collagen films, chitosan plus gelatin-based films, and, to a much lesser extent, chitosan-based films. By using autofluorescence densitometry of extracellular matrix, the authors were able to quantify the degree of connective tissue regeneration in the dura mater following duraplasty.


RESUMEN: Se estudió la regeneración de la duramadre después de una duraplastía utilizando una lámina de colágeno, una lamina de quitosano o una combinación de ambas con gelatina en un modelo de craneotomía y lesión cerebral en ratas. La autofluorescencia del colágeno en la duramadre regenerada se evaluó mediante microscopía confocal con excitación a λem = 488 nm y λem = 543 nm. Se observó un aumento en la regeneración de la matriz extracelular del tejido conectivo y un aumento en la fluorescencia de la matriz a las 6 semanas después de la duraplastía. Se observe un efecto significativo en la regeneración de la duramadre con las láminas de colágeno, las láminas en base de quitosano más gelatina y, en un menor grado, las láminas a base de quitosano. Mediante el uso de densitometría de autofluorescencia de la matriz extracelular, los autores lograron cuantificar el grado de regenera- ción del tejido conectivo en la duramadre después de la duraplastía.


Assuntos
Animais , Masculino , Ratos , Dura-Máter/anatomia & histologia , Dura-Máter/cirurgia , Dura-Máter/fisiologia , Craniectomia Descompressiva , Regeneração , Densitometria , Quitosana , Modelos Animais de Doenças , Fluorescência
10.
Rev. cuba. med ; 60(3): e1679, 2021.
Artigo em Espanhol | LILACS, CUMED | ID: biblio-1347521

RESUMO

La desproporcional y alta frecuencia de órdenes médicas de anticuerpos frente al citoplasma del neutrófilo (ANCA, por sus siglas en inglés) dirigidas a nuestros laboratorios clínicos evidencia el sobreuso de la prueba de ANCA. El uso indiscriminado de esta aumenta los gastos sin beneficio de salud. El laboratorio clínico es el eslabón de la cadena diagnóstica que más siente el uso excesivo de las solicitudes de ANCA, básicamente porque genera resultados falsos positivos que comprometen la utilidad clínica de la prueba, además de recargar innecesariamente el trabajo diario del laboratorio. La prueba de ANCA es una herramienta diagnóstica muy útil para las vasculitis sistémicas primarias, pero su valor en situaciones no vasculíticas así como en otras condiciones inflamatorias y en enfermedades infecciosas o tumorales, no ha sido demostrado.1,2 El descubrimiento de los ANCA cambió...(AU)


Assuntos
Humanos , Anticorpos Anticitoplasma de Neutrófilos , Técnicas de Laboratório Clínico/métodos , Vasculite Sistêmica , Fluorescência
11.
J Clin Virol Plus ; 1(1): 1-2, 2021.
Artigo em Inglês | LILACS, CONASS, Coleciona SUS, Sec. Est. Saúde SP, SESSP-IALPROD, Sec. Est. Saúde SP | ID: biblio-1416776

RESUMO

Introduction Brazil is the second largest country with COVID-19 positive cases worldwide. Due to the potent spread of the virus and the scarcity of kits and supplies, the Brazilian Ministry of Health has granted authorization for the use of kits available during this emergency, without an accurate evaluation of their performance. This study compared the performance and cost-effectiveness of seven molecular assays/kits available in São Paulo, Brazil, for SARS-CoV-2 diagnosis Materials and methods A total of 205 nasopharyngeal/oropharyngeal samples from suspected cases of COVID-19, were tested using the following assays: (i) GeneFinder COVID-19 plus RealAmp kit; (ii) 2019-nCoV RNA PCR-Fluorescence Probing, Da An Gene Co.; (iii) in-house RT-qPCR SARS-CoV-2 IAL; (iv) 2019-nCoV kit, IDT; (v) molecular SARS-CoV-2 (E) kit, Bio-Manguinhos; (vi) Allplex 2019-nCoV modified Assay, Seegene Inc, and (vii) Biomol one-step COVID-19 kit, IBMP. The criteria for determining a SARS-CoV-2 true positive result included the cycle threshold cut-off values, the characteristics of exponential/linear curves, the gene target diversity, and a positive result in at least two assays Results The overall sensitivity of the assays listed were GeneFinder 83.6%, Da An Gene 100.0%, IAL 90.4%, IDT 94.6%, Bio-Manguinhos 87.7%, Allplex 97.3%, and IBMP 87.7%. The minor sensitive gene target was RdRP. Although all assays had a Cohen's Kappa index ≥0.893, the best tests used multiplex assays identifying N-gene and/or E-gene targets Conclusion All assays tested accurate for diagnosis, but considering cost-effectiveness (cost, time consumption, number of samples tested, and performance), the in-house IAL assay was ideal for COVID-19 diagnosis in São Paulo, Brazil.


Assuntos
Vírus , Custos e Análise de Custo , Equipamentos e Provisões , Fluorescência
12.
Einstein (Säo Paulo) ; 19: eRC5638, 2021. graf
Artigo em Inglês | LILACS | ID: biblio-1249744

RESUMO

ABSTRACT Peri-implant diseases, caused by bacteria from biofilm related to dental implants, are one of the main causes of late loss of implants. In this sense, peri-implant diseases are divided into peri-implant mucositis, when it affects only the soft tissues, and peri-implantitis, when there is a bone involvement, which can lead to the failure of dental implant therapy. Thus, biofilm removal is essential for peri-implant health, allowing long-term success in implant therapy. To improve the visualization of oral biofilm, which is usually transparent or colorless, disclosing agents have been routinely used. However, disclosing agents have allergenic potential and can cause staining extrinsically in restorative and prosthetic materials, leading to aesthetic impairment. Thus, the use of fluorescence has been studied as an alternative for visualization of oral biofilm. Therefore, this report describes the use of wide-field optical fluorescence for visualization of oral biofilm associated with implants and teeth, in a routine appointment and follow-up of a partially edentulous patient with peri-implant mucositis. In addition, this report showed wide-field optical fluorescence can be used in a clinical routine of care of patients with dental implants. In this sense, wide-field optical fluorescence allowed easy and immediate visualization of the mature oral biofilm for its adequate removal, evaluation of the quality of restoration to sealing of screw access-hole of implant and identification of cariogenic lesions, without risk of allergic reactions or staining of prostheses and restorations.


RESUMO Doenças peri-implantares, causadas por bactérias de biofilme relacionadas a implantes dentários, são uma das principais causas de perda tardia de implantes. Nesse sentido, as doenças peri-implantares são divididas em mucosite peri-implantar, quando afeta apenas tecidos moles, e peri-implantite, quando há comprometimento ósseo, o que pode levar ao fracasso da terapia com implantes dentários. Assim, a remoção do biofilme é essencial para a saúde peri-implantar, permitindo sucesso a longo prazo na terapia com implantes. A fim de melhorar a visualização do biofilme oral, que geralmente é transparente ou incolor, agentes reveladores têm sido rotineiramente utilizados. No entanto, esses agentes têm potencial alergênico e podem causar manchas extrinsecamente em materiais restauradores e protéticos, levando a prejuízo estético. Assim, o uso da fluorescência tem sido estudado como alternativa para visualização do biofilme oral. Este relato descreve o uso da fluorescência óptica de campo amplo para visualização do biofilme oral associado a implantes e dentes em uma consulta de acompanhamento de rotina de uma paciente parcialmente edêntula com mucosite peri-implantar. Além disso, este relato evidenciou que a fluorescência óptica de campo amplo pode ser utilizada dentro da rotina clínica de atendimento de pacientes com implantes dentários. Nesse sentido, a fluorescência óptica de campo amplo permitiu a visualização fácil e imediata do biofilme oral maduro para sua remoção adequada, a avaliação da qualidade da restauração do selamento do orifício de acesso do parafuso do implante e a identificação de lesões cariogênicas, sem risco de reações alérgicas ou manchamento de próteses e restaurações.


Assuntos
Humanos , Implantes Dentários/efeitos adversos , Mucosite , Peri-Implantite/etiologia , Peri-Implantite/diagnóstico por imagem , Biofilmes , Fluorescência
13.
Gac. méd. boliv ; 43(2): 120-126, dic. 2020. ilus
Artigo em Espanhol | LILACS | ID: biblio-1249991

RESUMO

En diferentes regiones de Latinoamérica la infección por T. cruzi y Leishmania se superponen, por lo cual se reportan infecciones mixtas circulantes, debido a esto; deben realizarse pruebas diagnósticas específicas para evitar reacciones cruzadas entre estas dos patologías. OBJETIVO: determinar patrones de fluorescencia que permitan la diferenciación entre Leishmaniasis, enfermedad de Chagas e infección mixta empleando epimastigotes de T. cruzi. MÉTODOS: se empleó la técnica de Inmunofluorescencia Indirecta utilizando epimastigotes de T. cruzi (TcV autóctono) como antígeno figurado frente a un panel de muestras de suero codificados como A, B, C y D correspondientes a pacientes con infección por: Leishmaniasis (A), Infección mixta por Leishmania y Chagas(B), Enfermedad de Chagas (C) y sin ninguna de las dos infecciones (D). RESULTADOS: en los cuatro paneles de muestras se observaron diferentes patrones de intensidad de fluorescencia a nivel de membrana y núcleo de los epimastigotes de T. cruzi (TcV autóctono). CONCLUSIONES: la técnica de Inmunofluorescencia (IFI) con antígenos de epimastigotes de T. cruzi a demostrado utilidad en la diferenciación entre enfermedad de Chagas, Leishmaniasis y/o infecciones mixtas por ambos parásitos en aquellas zonas donde la coexistencia de ambas es habitual


In different regions of Latin America, infection by T. cruzi and Leishmania overlap, for which mixed circulating infections are reported, due to this; Specific diagnostic tests must be performed to avoid cross reactions between these two pathologies. OBJECTIVE: to determine fluorescence patterns that allow the differentiation between Leishmaniasis, Chagas disease and mixed infection using T. cruzi epimastigotes. METHODS: the Indirect Immunofluorescence technique was used using epimastigotes of T. cruzi (autochthonous TcV) as figurative antigen against a panel of serum samples coded as A, B, C and D corresponding to patients with infection by: Leishmaniasis (A) , Mixed infection by Leishmania and Chagas (B), Chagas disease (C) and without either of the two infections (D). RESULTS: in the four sample panels, different patterns of fluorescence intensity were observed at the membrane and nucleus level of the epimastigotes of T. cruzi (autochthonous TcV). CCONCLUSIONS: the Immunofluorescence technique (IFI) with T. cruzi epimastigote antigens has proven useful in differentiating between Chagas disease, Leishmaniasis and / or mixed infections by both parasites in areas where the coexistence of both is common.


Assuntos
Humanos , Trypanosoma cruzi , Leishmaniose , Fluorescência , Parasitos , Doença de Chagas , Infecções
14.
Int. j. morphol ; 38(5): 1485-1495, oct. 2020. graf
Artigo em Inglês | LILACS | ID: biblio-1134466

RESUMO

SUMMARY: Axolotl limb regeneration is a fascinating characteristic that has attracted attention for several decades. Our previous studies on axolotl limb regeneration indicated that the satellite cells in the remnant muscles move distally into the blastema to regenerate new muscles that are separated by a gap from remnant muscles. Thereafter, the regenerative muscle fibers start to reconnect with remnant ones. In this study, the reconnection at the individual muscle fiber level was elucidated to test the hypothesis that this reconnection happens synchronously among involved muscles. Three pairs of EGFP+ mid-bud stage blastemas were transplanted onto freshly amputated stumps of RFP+ axolotls at the same thigh position to generate double fluorescence chimeric regenerative hindlimbs. These regenerative limbs were harvested very late far beyond they had reached the late differentiation stage. Fluorescence imaging of these limbs in cross sections revealed that in the proximal remnant part of the muscle fiber, reconnection occurred at a different pace among the muscles. In the major thigh muscle gracilis, the reconnection started from the periphery before it was completed. Furthermore, RFP+ muscle fibers contributed to muscle regeneration in the distal regenerative parts. Intriguingly, this red cell contribution was limited to ventral superficial muscles of the calf. This kind of double fluorescence chimeric limb regeneration model may help increase the understanding of the patterning of axolotl limb regeneration in late stages.


RESUMEN: La regeneración del miembro de Axolotl es una característica fascinante que ha llamado la atención durante varias décadas. Nuestros estudios previos sobre la regeneración del miembro del Axolotl indicaron que las células satélite en los músculos remanentes se mueven distalmente hacia el blastema para regenerar nuevos músculos que están separados por una brecha de músculos remanentes. A partir de entonces, las fibras musculares regenerativas comienzan a reconectarse con las restantes. En este estudio, se aclaró la reconexión a nivel de fibra muscular individual para probar la hipótesis de que esta reconexión ocurre sincrónicamente entre los músculos involucrados. Se trasplantaron tres pares de blastemas EGFP+ en la etapa de yema media en tocones recién amputados de axolotls RFP+ en la misma posición del muslo para generar miembros posteriores regenerativos quiméricos de fluorescencia doble. Estos miembros regenerativos se cosecharon muy tarde mucho más allá de haber alcanzado la etapa de diferenciación tardía. Las imágenes de fluorescencia de estos miembros en secciones transversales revelaron que en la parte remanente proximal de la fibra muscular, la reconexión se produjo a un ritmo diferente entre los músculos. En el músculo grácil, la reconexión comenzó desde la periferia antes de completarse. Además, las fibras musculares RFP+ contribuyeron a la regeneración muscular en las partes regenerativas distales. Curiosamente, esta contribución de glóbulos rojos se limitó a los músculos superficiales ventrales de la pantorrilla. Este tipo de modelo de regeneración quimérica de doble fluorescencia del miembro puede ayudar a aumentar la comprensión del patrón de la regeneración del miembro del Axolotl en etapas tardías.


Assuntos
Animais , Regeneração/fisiologia , Extremidades/fisiologia , Ambystoma mexicanum/fisiologia , Animais Geneticamente Modificados , Transplante de Células , Fluorescência
15.
Arq. bras. neurocir ; 39(3): 207-212, 15/09/2020.
Artigo em Inglês | LILACS | ID: biblio-1362422

RESUMO

Metastasis to the calvarium with direct pericranium or dural infiltration may be treated with radical surgical removal in selected cases. We describe microsurgical resection of calvarial metastases with fluorescence-guided technique using 5-aminolevulinic acid (5-ALA) in two female patients with breast cancer. Fluorescence findings were positive in both cases. Margins in the scalp and dural layer were 5-ALA negative at the end of surgical removal. Intraoperative pathology was performed in all cases to confirm if oncological limits were free of disease. One case was 5-ALA positive in the outer layer of the dura-mater and another in the pericranium. At the end of the removal in both cases, the surgicalmargins were 5-ALA fluorescence-free. Intraoperative pathology confirmed oncological limits of the resection. 5-aminolevulinic acid fluorescence-guided surgery for calvarial metastases with pericranium and/or dural extension seems to be a safe and reliable method to aid the surgical margins for complete removal, possibly delaying or avoiding adjuvant irradiation for progression control.


Assuntos
Neoplasias da Base do Crânio/cirurgia , Fluorescência , Ácido Aminolevulínico , Metástase Neoplásica , Crânio/anormalidades , Crânio/cirurgia , Estudos Retrospectivos , Neoplasias da Base do Crânio/diagnóstico , Margens de Excisão
16.
Rev. Inst. Adolfo Lutz ; 79: 1-5, 31 mar. 2020. ilus, tab
Artigo em Português | LILACS, CONASS, Coleciona SUS, Sec. Est. Saúde SP, SESSP-ACVSES, SESSP-IALPROD, Sec. Est. Saúde SP, SESSP-IALACERVO | ID: biblio-1342948

RESUMO

A incerteza de medição representa o nível de confiança no resultado. Para a estimativa da incerteza de medição foi empregado o cálculo do desvio padrão da reprodutibilidade intralaboratorial de 48 ensaios de contagem de bactérias heterotróficas pela técnica da membrana filtrante com detecção por fluorescência pelo uso de substrato fluorogênico em amostras de água purificada contaminadas artificialmente entre 10 e 100 UFC/mL. O valor obtido, 1,3 x 10-3 (log10), indica que a técnica utilizada pode ser uma alternativa para a estimativa da incerteza de medição em ensaios microbiológicos quantitativos de contagem de bactérias heterotróficas em amostras de água purificada. (AU)


Measurement uncertainty represents the confidence level in the result. To estimate the expanded measurement uncertainty, the standard deviation of intra-laboratory reproducibility of 48 heterotrophic bacterial count assays by fluorescence detection by the use of fluorogenic substrate on artificially contaminated purified water samples between 10 and 100 CFU/mL was used. The value obtained, 1.3 x 10-3 (log10), indicates that the technique used can be an alternative to estimate measurement uncertainty in quantitative microbiological heterotrophic bacterial count assays in purified water samples using fluorogenic substrate. (AU)


Assuntos
Contagem de Colônia Microbiana , Purificação da Água , Incerteza , Bactérias Heterotróficas , Fluorescência
17.
Rev. Inst. Adolfo Lutz ; 79: e1784, 31 mar. 2020. ilus, tab
Artigo em Português | LILACS, VETINDEX | ID: biblio-1489616

RESUMO

A incerteza de medição representa o nível de confiança no resultado. Para a estimativa da incerteza de medição foi empregado o cálculo do desvio padrão da reprodutibilidade intralaboratorial de 48 ensaios de contagem de bactérias heterotróficas pela técnica da membrana filtrante com detecção por fluorescência pelo uso de substrato fluorogênico em amostras de água purificada contaminadas artificialmente entre 10 e 100 UFC/mL. O valor obtido, 1,3 x 10-3 (log10), indica que a técnica utilizada pode ser uma alternativa para a estimativa da incerteza de medição em ensaios microbiológicos quantitativos de contagem de bactérias heterotróficas em amostras de água purificada.


Measurement uncertainty represents the confidence level in the result. To estimate the expanded measurement uncertainty, the standard deviation of intra-laboratory reproducibility of 48 heterotrophic bacterial count assays by fluorescence detection by the use of fluorogenic substrate on artificially contaminated purified water samples between 10 and 100 CFU/mL was used. The value obtained, 1.3 x 10-3 (log10), indicates that the technique used can be an alternative to estimate measurement uncertainty in quantitative microbiological heterotrophic bacterial count assays in purified water samples using fluorogenic substrate.


Assuntos
Bactérias Heterotróficas/análise , Carga Bacteriana/métodos , Incerteza , Purificação da Água , Fluorescência
18.
Einstein (Säo Paulo) ; 18: eAO4954, 2020. graf
Artigo em Inglês | LILACS | ID: biblio-1056032

RESUMO

ABSTRACT Objective: To evaluate the magnetic hyperthermia therapy in glioblastoma tumor-on-a-Chip model using a microfluidics device. Methods: The magnetic nanoparticles coated with aminosilane were used for the therapy of magnetic hyperthermia, being evaluated the specific absorption rate of the magnetic nanoparticles at 300 Gauss and 305kHz. A preculture of C6 cells was performed before the 3D cells culture on the chip. The process of magnetic hyperthermia on the Chip was performed after administration of 20μL of magnetic nanoparticles (10mgFe/mL) using the parameters that generated the specific absorption rate value. The efficacy of magnetic hyperthermia therapy was evaluated by using the cell viability test through the following fluorescence staining: calcein acetoxymethyl ester (492/513nm), for live cells, and ethidium homodimer-1 (526/619nm) for dead cells dyes. Results: Magnetic nanoparticles when submitted to the alternating magnetic field (300 Gauss and 305kHz) produced a mean value of the specific absorption rate of 115.4±6.0W/g. The 3D culture of C6 cells evaluated by light field microscopy imaging showed the proliferation and morphology of the cells prior to the application of magnetic hyperthermia therapy. Fluorescence images showed decreased viability of cultured cells in organ-on-a-Chip by 20% and 100% after 10 and 30 minutes of the magnetic hyperthermia therapy application respectively. Conclusion: The study showed that the therapeutic process of magnetic hyperthermia in the glioblastoma on-a-chip model was effective to produce the total cell lise after 30 minutes of therapy.


RESUMO Objetivo: Avaliar a terapia de magneto-hipertermia em modelo de tumor de glioblastoma on-a-Chip. Métodos: As nanopartículas magnéticas recobertas com aminosilana foram utilizadas para a terapia da magneto-hipertermia, sendo avaliada a taxa de absorção específica das nanopartículas magnéticas em 300 Gauss e 305kHz. Uma pré-cultura de células C6 foi realizada e, seguidamente, foi feito o cultivo das células 3D no chip. O processo de magneto-hipertermia no chip foi realizado após administração de 20μL de nanopartículas magnéticas (10mgFe/mL), utilizando os parâmetros que geraram o valor da taxa de absorção específica. A eficácia da terapia de magneto-hipertermia foi avaliada pela viabilidade celular por meio dos corantes fluorescentes acetoximetiléster de calceína (492/513nm), para células vivas, e etídio homodímero-1 (526/619nm), para células mortas. Resultados: As nanopartículas magnéticas, quando submetidas ao campo magnético alternado (300 Gauss e 305kHz), produziram um valor médio da taxa de absorção específica de 115,4±6,0W/g. A cultura 3D das células C6 avaliada por imagem de microscopia de campo claro mostrou a proliferação e a morfologia das células antes da aplicação da terapia de magneto-hipertermia. As imagens de fluorescência mostraram diminuição da viabilidade das células cultivadas no organ-on-a-Chip em 20% e 100% após 10 e 30 minutos, respectivamente, da aplicação da terapia de magneto-hipertermia. Conclusão: O processo terapêutico da magneto-hipertermia no modelo de tumor glioblastoma on-a-chip foi eficaz para produzir lise total das células após 30 minutos de terapia.


Assuntos
Animais , Ratos , Glioblastoma/terapia , Técnicas de Cultura de Células/métodos , Dispositivos Lab-On-A-Chip , Nanopartículas de Magnetita/uso terapêutico , Hipertermia Induzida/métodos , Temperatura , Fatores de Tempo , Sobrevivência Celular , Reprodutibilidade dos Testes , Resultado do Tratamento , Linhagem Celular Tumoral , Campos Magnéticos , Fluorescência
19.
Araçatuba; s.n; 2020. 43 p. graf, ilus.
Tese em Português | LILACS, BBO - Odontologia | ID: biblio-1396449

RESUMO

A remoção de facetas de resina composta quando o retratamento se faz necessário representa um desafio ao clínico no sentido de preservação do remanescente dental. Sendo assim, o objetivo deste estudo foi avaliar dispositivos auxiliares na precisão dos retratamentos de facetas diretas, por meio de escaneamento digital. Setenta e cinco espécimes de dente bovino (10 x 8 mm ­ cor A1) foram selecionados, e então foi realizado o escaneamento inicial (T0). Em seguida, os espécimes foram preparados para faceta de resina composta e novamente escaneados (T1) para verificação da padronização dos preparos. As superfícies dentárias foram então restauradas com sistema adesivo e resina composta. Os espécimes foram divididos em 5 grupos (n=15): remoção sem dispositivo auxiliar (CN ­ grupo controle), remoção com alta rotação com LED branco (AL), remoção com alta rotação com luz ultra-violeta (UV), remoção com motor elétrico e multiplicadora 1/5 (MT) e remoção com uso de lupa de aumento 2,5x (MN). A remoção das facetas e o re-preparo dos espécimes foi feito por outro operador, sendo então realizado um novo escaneamento (T2). A alteração volumétrica entre T2 e T1, assim como, as áreas de desgaste dentário e/ou presença de resíduos de resina composta foram avaliados. A média entre desgaste e presença de resíduos entre T2 e T1 foi também realizada. Os dados foram submetidos aos testes estatísticos de Kruskal-Wallis e pós teste de Dunn ao nível de significância de 5%. Não foi encontrada diferença estatística entre os grupos entre T1 e T0, com uma média de desgaste de 0,28 mm (± 0,07), p ≥ 0.05. Somente houve diferença entre os grupos para as análises referentes às áreas de desgaste e de presença de resíduos (p ≤ 0,05). Ocorreu maiores áreas de desgaste para o grupo MN, sendo estatisticamente superior aos grupos CN e MT (p≤ 0,05). O oposto ocorreu para áreas de resíduos, sendo que o grupo MN apresentou as menores áreas de resíduos, sendo estatisticamente inferior aos grupos CN e MT (p≤ 0,05). Considerando a média entre desgaste e resíduos, ocorreu um desgaste adicional após o re-preparo, indepedente do grupo (p≥ 0,05). Apesar do uso de lupa odontológica ser mais efetivo para remoção da resina composta durante o retratamento de faceta direta, este proporciona maior desgaste da estrutura dentária. A similaridade da fluorescência entre a resina composta e o substrato dentário minimizou os benefícios do dispositivo com luz UV(AU)


The removal of direct composite veneers when the retreatment is necessary represents a challenge to the clinician in the sense of dental preservation. Thus, the aim of this study was to evaluate auxiliary devices, in the precision of direct composite veneers retreatments, through digital scanning. Seventy-five bovine tooth specimens (10 x 8 mm - A1 shade) were selected and the initial scanning (T0) was performed. Then, the specimens were prepared for direct composite veneer and rescanned (T1) to verify the standardization of the preparations. The dental surfaces were then restored with adhesive system and composite resin. The specimens were divided into 5 groups (n=15): removal without auxiliary device (CN - control group), removal with a hand piece with a white LED (WL), removal with a hand piece with an UV light source (UV), removal with electric motor and multiplier 1/5 hand piece (MT); and removal with the use of a 2.5x dental loupe (MN). The removal of the restorations and the re-preparation of the specimens were done by another operator, and a new scanning (T2) was performed. The volumetric change between T2 and T1, as well as the areas of additional dental wear and presence of composite resin residues were evaluated. The mean considering additional wear and the presence of residues between T2 and T1 were also performed. The data were submitted to Kruskal-Wallis and Dunn's as post-test, at significance level of 5%. No statistical difference among the groups were found between T1 and T0 with a wear mean of 0.28 mm (±0.07), p≥ 0.05. There were only statistical differences between the groups for the analyses related to the areas of wear and presence of residues (p≤ 0.05). There were greater areas of wear for MN group, being statistically superior to CN and MT groups (p≤ 0.05). The opposite occurred for areas of presence of residues, with the MN group presenting smaller areas of residues, being statistically lower than the CN and MT groups (p≤ 0.05). Considering the average between wear and residues, additional wear occurred after re-preparation, regardless of the group (p≥ 0.05). Although the use of dental loupe is more effective for removal of composite resin during the retreatment of direct composite veneers, it provides greater wear of the dental structure. The fluorescence similarity between the composite resin and the dental substrate minimized the benefits of UV light device(AU)


Assuntos
Equipamentos Odontológicos de Alta Rotação , Retratamento , Instrumentos Odontológicos , Facetas Dentárias , Resinas Sintéticas , Raios Ultravioleta , Cor , Resinas Compostas , Cimentos Dentários , Esmalte Dentário , Dentina , Desgaste dos Dentes , Fluorescência
20.
Braz. j. med. biol. res ; 53(3): e8960, 2020. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1089336

RESUMO

This research aimed to explore the molecular mechanism of microRNA (miR)-106b in cell apoptosis of atherosclerosis (AS). Human aortic endothelial cells (HAECs) were divided into control group, oxidized-low-density lipoproteins (ox-LDL) group, miR-106b NC+ox-LDL group, miR-106b mimics+ox-LDL group, miR-106b mimics+PTEN+ox-LDL group, and miR-106b mimics+empty+ox-LDL group. Real-time fluorescence quantitative polymerase chain reaction, cholecystokinin, TdT-mediated biotinylated nick end-labeling assay, luciferase reporter gene assay, and flow cytometry analysis were performed to determine the morphology, proliferation, and apoptosis in HSECs. Moreover, the levels of phosphatase and tensin homolog deleted on chromosome 10 (PTEN), Bcl-2, p-P13K, and p-AKT in HAECs were detected by western blot. MiR-106b was down-regulated in ox-LDL-induced HAECs. PTEN was the target gene of miR-106b-5p. Overexpression of PTEN inhibited the anti-apoptotic effect of miR-106b. Compared with the control group, the proportion and number of HAECs apoptosis and Bax, caspase-3, and caspase-9 expression in ox-LDL and miR-106b mimics+PTEN+ox-LDL groups were significantly increased (all P<0.05). Moreover, the activity of HAECs and Bcl-2 were decreased significantly (all P<0.05). Overexpression of miR-106b in ox-LDL-induced AS inhibited endothelial cell apoptosis. Furthermore, miR-106b might activate the PI3K/AKT pathway by down-regulating the expression of PTEN in ox-LDL-induced HAECs.


Assuntos
Humanos , Apoptose , MicroRNAs/genética , Células Endoteliais/metabolismo , Aterosclerose/metabolismo , Lipoproteínas LDL/genética , Transdução de Sinais , Regulação para Cima , Proliferação de Células , Reação em Cadeia da Polimerase em Tempo Real , Fluorescência , Lipoproteínas LDL/metabolismo
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