Carcinosinumproduces phenotypic changes of 4T1 tumor cells in vitro

Considering that there are few published studies that specifically address the exclusive use of Carcinosinumin different potencies and, most of them focused on genotypic and clinical effects, the present study was proposed to identify possible phenotypic changes, including viability, expression of HER-2 and metastatic abilities, using 4T1 cells in vitroas a model. Carcinosinum was tested in different homeopathic potencies (12cH; 30cH; 200cH) mechanically prepared using sterile pure water. The time space between preparing the potencies and using them was 24 hours.The final dilutions were inserted into the culture medium in a volume equal to 10%, at the time of cell seeding. The same succussed vehicle used to prepare the drugs (70% ethanol) diluted 1:100 in sterile pure water was used as control. All treated cells were cultured in 25 mL flasks, with cell density of 5 x 105cells/mL. After 24 hours of treatment, cells were analyzed for apoptosis index using Annexin V kit and the Countess® system. The morphology of 4T1 cells was monitored by staining cell smears with hematoxylin-eosin and Giemsa methods. HER-2 expression was assessed by immunocytochemistry and metalloproteinase activity was assessed by zymography. The determination of the cytokine profile was performed using Cytometric Bead Array (CBA). The samples were evaluated in quadruplicate and the data were analyzed by one-way ANOVA. Carcinosinum30cH presented the highest apoptotic index and reduction of MMP-9-Pro expression; Carcinosinum200cH produced the highest positivity for HER-2 and no specific effect was seen after the treatment with Carcinosinum12cH. No change in cytokine expression was seen among treatments. We conclude that Carcinosinum30cH and 200cH can change phenotypic features important totumor development in vitro. The clinical meaning of these data deserves further investigation.

Preparation of Cancer Nosodes from specific cancer tissues

Nosodes are ultra-dilute preparations made from diseased tissues or organisms used in homeopathy since 1830, more frequently used since 1880. Carcinosin is a commonly used nosode prepared over 100 years ago from cancer tissues in the last part of the nineteenth century, of which the exact scientific information on the histopathological characterization of the source material is not available. The current therapeutic indications of Carcinosin have been largely empirical and clinically derived. In such a scenario, understanding the paramount importance of the Cancer nosode, it's revamping in the light of current scientific knowledge has been identified by the author. Specific cancerous tissue samples were sourced for nosode preparation. Identified pieces of tissue were histopathologically diagnosed, thoroughly washed, quantified and triturated with powder of Saccharum lactis to prepare decimal potencies, as per standard method suggested in the Homoeopathy Pharmacopoeia of India. Centesimal potencies were prepared using electromechanical potentizer, documenting the force parameters. The Cancer nosodes from specific cancer tissues were prepared in a systematic manner, which could be used for research as well as in clinical practice. The author has attempted scientific preparation of new Cancer nosodes making them available to the profession. On similar lines, more of cancer tissues could be explored for making other nosodes. (AU)

Personalidad sobreadaptada y homeopatía / Personality sobreadaptada and homeopathy

El presente trabajo está destinado a describir una estructura de personalidad que se observa cada vez con mayor frecuencia, muy favorecida por la actual “cultura del éxito”. Quienes la presentan son personas que sostienen un excesivo ajustea la realidad exterior y a sus exigencias, disociando necesidades y posibilidades emocionales y corporales. Se establece la relación del cuadro psicopatológico con algunos remedios homeopáticos.

This paper is intended to describe a personality structure that is observed with increasing frequency, favored by the current "culture of success". Those who have are people who argue overshootingto external reality and demands decoupling emotional and physical needs and possibilities. The relationship of psychopathology with some homeopathic remedies set.

Una historia clínica muy discutida / A medical record very discussed

Voy a referir una historia clínica de nuestra cátedra que ha dado lugar a opiniones controvertidas y las reflexiones que he sugerido a los colegas en casos clínicos como éste

Un caso de Carcinosin en un gato / Carcinosin in a cat

Se presenta un raro caso de un gato joven, con patología tumoral grave que reaccionó favorablemente a la prescripción de Carcinosin

Carcinosinum / Carcinosinum

Este trabajo trata sobre el nosode del cáncer: Carcinosinum. En él se detalla la dinámica mental que caracteriza al paciente Carcinosin, sus síntomas generales y particulares destacables. Se establecen los diferentes diagnósticos a tener en cuenta para su correcta prescripción. Por último se hace hincapié que no solo se debe administrar Carcinosin como sinónimo de cáncer sino que hay que tenerlo también presente en todas aquellas enfermedades proliferativas y destructivas con escasa psora o reacción donde Carcinosin está perfectamente indicado

Aspectos clinicos e farmacologicos de Carcinosinum / Clinical and pharmacological aspects of Carcinosinum

Dois portadores de verrugas disseminadas foram beneficiados pelo Carcinosinum, prescrito segundo a imagem de patogenesias publicadas. A analise critica dos casos questiona a validade da terapeutica adotada, por falta de padronizacao deste nosodio, o qual vem sofrendo sucessivas variacoes quanto ao tumor de origem, representando uma substancia-base enigmatica. O medicamento sera forcosamente abolido do receituario homeopatico e dos programas de ensino, pela impossibilidade de argumentacao dos eventuais bons resultados obtidos