Highly Resolved Genomes of Two Closely Related Lineages of the Rodent Louse Polyplax serrata with Different Host Specificities.

Sucking lice of the parvorder Anoplura are permanent ectoparasites with specific lifestyle and highly derived features. Currently, genomic data are only available for a single species, the human louse Pediculus humanus. Here, we present genomes of two distinct lineages, with different host spectra, of a rodent louse Polyplax serrata. Genomes of these ecologically different lineages are closely similar in gene content and display a conserved order of genes, with the exception of a single translocation. Compared with P. humanus, the P. serrata genomes are noticeably larger (139 vs. 111 Mbp) and encode a higher number of genes. Similar to P. humanus, they are reduced in sensory-related categories such as vision and olfaction. Utilizing genome-wide data, we perform phylogenetic reconstruction and evolutionary dating of the P. serrata lineages. Obtained estimates reveal their relatively deep divergence (∼6.5 Mya), comparable with the split between the human and chimpanzee lice P. humanus and Pediculus schaeffi. This supports the view that the P. serrata lineages are likely to represent two cryptic species with different host spectra. Historical demographies show glaciation-related population size (Ne) reduction, but recent restoration of Ne was seen only in the less host-specific lineage. Together with the louse genomes, we analyze genomes of their bacterial symbiont Legionella polyplacis and evaluate their potential complementarity in synthesis of amino acids and B vitamins. We show that both systems, Polyplax/Legionella and Pediculus/Riesia, display almost identical patterns, with symbionts involved in synthesis of B vitamins but not amino acids.

A new genus and new species of sucking louse (Phthiraptera: Anoplura: Enderleinellidae) from the particoloured flying squirrel (Hylopetes alboniger) in Laos, and an updated identification key to enderleinellid genera.

Hylophthirus spinosus, new genus and new species (Phthiraptera: Anoplura: Enderleinellidae), is described from specimens collected from the particoloured flying squirrel, Hylopetes alboniger in Khammouane Province, Laos (Lao Peoples Democratic Republic). Both sexes of the new louse are illustrated with stacked microphotographs, scanning electron micrographs and line drawings. An updated morphological identification key to the six genera (Atopophthirus, Enderleinellus, Hylophthirus, Microphthirus, Phthirunculus and Werneckia) now recognised within the family Enderleinellidae is presented. The new genus is unique within the Enderleinellidae in having massive spines (modified setae) on some abdominal sternites, partially bulbous 5th antennal segments, mesothoracic spiracles borne on protuberances and the morphology of the genitalia of both sexes. Tables showing all genera of sucking lice that include species parasitising sciurids (squirrels, chipmunks, susliks and marmots), and all known species of enderleinellids, with their known host associations and geographical distributions are included and briefly discussed in relation to the new genus and species.

Revision of the bark louse genus Bicuspidatus (Psocodea: Peripsocidae) with description of a new species from China.

Bicuspidatus is a small genus of the family Peripsocidae, erected by Li (1993) with B. pulchipunctatus as the type species. Adults of this genus can be easily distinguished from other peripsocids by the phallosome with bicuspid apical process in males or by the subgenital plate with modestly elongated, distally bilobed posterior lobe in females. Bicuspidatus currently comprises three species: B. guangdongensis Li, 1993, B. pulchipunctatus Li, 1993 and B. sigillatus Li, 1993, all are known only from Guangdong Province, China. According to previous studies, these three species can be distinguished by the markings on the forewings and a few characteristics of phallosome (Li 1993, 2002). In this study, we present a taxonomic revision of Bicuspidatus based on examination of 40 specimens collected from various localities, including the type specimens of B. guangdongensis, B. pulchipunctatus and B. sigillatus. Furthermore, 12 of them were used for molecular analyses based on partial sequences of COI gene. As a result, B. guangdongensis and B. sigillatus are here considered as junior synonyms of B. pulchipunctatus. We redescribe B. pulchipunctatus and describe a new species from Xizang Autonomous Region, China. An updated distribution map and a revised key to the species of Bicuspidatus are also provided.

Parasitic findings on threatened pudu deer from Central Chile accounts first genetic characterization of lice parasitizing P. puda in Chile and the first molecular report of Taenia hydatigena metacestodes in this species.

Southern pudu (Pudu puda) is a threatened endemic deer of the temperate forests of Chile. In recent years pudu populations rates have decreased mainly due to anthropogenic causes including forest loss and landscape fragmentation. In this context, the parasitic fauna of Chilean pudu has been scarcely investigated. The aim of this study was to determine the parasitic status of rescued pudu n = 13 from its natural habitat in Central Chile (Maule region) during March 2022 and June 2023 by applying morphological, histopathological, and molecular analyses. As result, we report the presence of transmission of parasites from dogs to pudus as showed by the presence of metacestodes of the parasite Taenia hydatigena on omentum, liver, and pleura of pudus during postmortem examinations, being the first molecular report on the presence of this parasite on Chilean pudu. Meanwhile, ectoparasite examinations determined the presence of chewing and sucking lice on pudu exemplars here analysed. Molecular and phylogenetic analysis of lice revealed new insights on Bovicola and Anoplura lice parasitizing P. puda in Chile, equally being the first genetic characterization of lice parasitizing pudu exemplars in Chile. In addition, parasite loads of lice and metacestodes were analysed. However, no statistically significance was observed when comparing environmental and individual traits influence on parasite load variation. Overall, the study area is the northern limit of habitat distribution of this specie in Chile and we here provide novel information on pudu deer parasites, thus making a useful and valuable contribution to the parasitological knowledge on this threatened species.

Health status of honeybee colonies (Apis mellifera) and disease-associated risk factors in different agroecological zones of Southwest Ethiopia.

A cross-sectional study design was conducted in different agroecological zones of southwest Ethiopia from October 2019 to October 2021. The study aimed to determine the prevalence and associated risk factors for honeybee diseases and pests, as well as the impact of these issues on honeybee colonies and their products. To identify potential risk factors for honeybee disease and pests, a multivariate random effects logistic regression analysis was used. Adult honeybee and brood samples from a total of 384 honeybee colonies were collected and tested using standard laboratory diagnostic methods. The highest prevalence (55.8%) of ants was recorded, followed by wax moths (22.5%) and hive beetles (23.3%). In the current study, the main honeybee diseases observed in the study areas were varroosis (36.5%), bee lice (5.2%), nosemosis (39.6%), amoeba (56%), and chalkbrood (4.5%). However, tracheal mites, sachbrood, and American and European foul brood, were not detected. The agroecological zone (OR = 5.2, 95% CI: 1.75-14.85), type of hive (OR = 2.9, 95% CI: 1.17-17.03), management system (OR = 4.3, 95% CI: 1.23-14.70), and the management of the colony (OR = 3.5, 95% CI: 1.31-9.14) were identified as risk factors for varroosis in these areas. The occurrence of nosemosis in colonies was also influenced by the agroecological zone (OR = 12.2, 95% CI: 3.06-48.54) and colony management (OR = 3.4, 95% CI: 1.59-7.23). The agroecological zone (OR = 10.5, 95% CI: 12.76-22.63) and hive type (OR = 3.0, 95% CI: 1.39-6.36) were the primary risk factors for the occurrence of amoeba in honeybee colonies. However, the occurrence of bee lice (OR = 34.7, 95% CI: 3.96-104.93) and chalkbrood (OR = 4.8, 95% CI: 1.44-13.16) in honeybee colonies was only influenced by the agroecological zone in the study areas. This study demonstrated that losses in honey production in the area are significantly attributed to honeybee disease and pests. Therefore, it is essential to increase public awareness of how honeybee diseases and pests affect honey production and to develop and implement appropriate control measures for these diseases and pests. Furthermore, more studies should be conducted to characterize and isolate other causes of honeybee diseases and pests in various locations.

Correlation of CT-based bone mineralization with drilling-force measurements in anatomical specimens is suitable to investigate planning of trans-pedicular spine interventions.

This interdisciplinary study examined the relationship between bone density and drilling forces required during trans-pedicular access to the vertebra using fresh-frozen thoraco-lumbar vertebrae from two female body donors (A, B). Before and after biomechanical examination, samples underwent high-resolution CT-quantification of total bone density followed by software-based evaluation and processing. CT density measurements (n = 4818) were calculated as gray values (GV), which were highest in T12 for both subjects (GVmaxA = 3483.24, GVmaxB = 3160.33). Trans-pedicular drilling forces F (Newton N) were highest in L3 (FmaxB = 5.67 N) and L4 (FmaxA = 5.65 N). In 12 out of 13 specimens, GVs significantly (p < 0.001) correlated with force measurements. Among these, Spearman correlations r were poor in two lumbar vertebrae, fair in five specimens, and moderately strong in another five specimens, and highest for T11 (rA = 0.721) and L5 (rB = 0.690). Our results indicate that CT-based analysis of vertebral bone density acquired in anatomical specimens is a promising approach to predict the drilling force appearance as surrogate parameter of its biomechanical properties by e.g., linear regression analysis. The study may be of value as basis for biomechanical investigations to improve planning of the optimal trajectory and to define safety margins for drilling forces during robotic-assisted trans-pedicular interventions on the spine in the future.

Distribution of phoretic mites and lice in Pseudolynchia canariensis living on pigeons and the relationship with seasonality, carrier sex, plumage coloration and age of definitive hosts.

Among the parasites, some groups that have a limited capacity for locomotion, such as mites and lice, the transmission is challenging to win. These ectoparasites disperse through direct contact between hosts or, in some cases, through phoresy. However, these processes are not well-documented in detail because they are difficult to observe and quantify. In the present study, the patterns of distribution of skin mites and phoretic lice on hippoboscid louse fly Pseudolynchia canariensis sampled from Columba livia were evaluated. The analyzed pigeons were juveniles and adults, with three distinct plumage colors: blue checker, spread, or wild type, and were caught over 24 months. A total of 1,381 hippoboscid flies were collected on 377 hosts. The plumage color did not influence the infestation patterns of louse flies on juvenile and adult pigeons, nor did it influence the infestation patterns of skin mites and phoretic lice on the hippoboscid flies. However, the environmental temperature was directly related to higher prevalence, mean infestation intensity, and phoretic species richness on P. canariensis during the hottest seasons. Furthermore, a higher abundance of phoretic mite eggs, including embryonated eggs, was observed in females of P. canariensis in all seasons.

Studies on the Determination of Ectoparasites and the Prevalence Rate in Cattle in Izmir, Aydin and Mugla Regions.

Objective: The aim of the study is to determine the prevalence rates of ectoparasites (external parasites) seen in cattle in Mugla, Aydin and Izmir regions. Methods: For ectoparasite screening, a total of 1,353 cattle breeds from Mugla, Aydin and Izmir regions were included in the study. Ectoparasite samples were collected with the help of hand, forceps, comb and scalpel, brought to the laboratory in plastic containers containing 70% alcohol and examined under a microscope. Results: In the study, 191 (14.12%) of 1,353 cattle were infested with external parasites. Of the cattle, 138 (72.25%) ticks, 24 (12.57%) lice, 9 (4.71%) tabanid, 8 (4.19%) hippoboscid, 7 (3.66%) fleas and 5 (2.62%) mites were detected. Accordingly, 4 tick species in cattle Hyalomma marginatum, Hyalomma excavatum, Hyalomma detritum, Rhipicephalus turanicus, 4 lice species (Bovicola bovis, Linognathus vituli, Solenopotes capillatus, Haematopinus quadripertusus ), 1 mite species (Chorioptes bovis), 2 flea species (Ctenocephalides canis, Ctenocephalides felis), 1 hippoboscid fly species (Hippobosca equina) ve 3 tabanid fly species (Tabanus bromius, Tabanus exclusus, Philipomyia aprica) diagnosed. Conclusion: The presence of external parasites varied depending on the frequency of antiparasitic use in cattle.

DO PARASITIC LICE EXHIBIT ENDEMISM IN PARALLEL WITH THEIR AVIAN HOSTS? A COMPARISON ACROSS NORTHERN AMAZONIAN AREAS OF ENDEMISM.

Areas of endemism are the smallest units in biogeography and can be defined as biologically unique areas comprising taxa with common geographic limits to their distributions. High beta diversity within Amazonia is often related to turnover among these areas. For decades, evolutionary biologists have tried to comprehend the mechanisms generating and maintaining the spatial structure and high diversity of free-living Amazonian organisms, particularly birds. However, few studies have tried to analyze these patterns among their parasites. Host and parasite associations involve shared history that may allow us to better understand the fine-scale evolutionary history of the host. Here we compare the coevolutionary patterns among 2 avian host species with distinct patterns of genetic structure in northern Amazonia, Dendrocincla fuliginosa (Aves: Dendrocolaptidae) and Dixiphia pipra (Aves: Pipridae), and their ectoparasitic lice (Insecta: Phthiraptera), Furnaricola sp. ex Dendrocincla fuliginosa, Myrsidea sp. ex Dixiphia pipra, and Tyranniphilopterus sp. ex Dixiphia pipra. We obtained sequences of the mitochondrial gene cytochrome oxidase subunit I from hosts and parasites collected on opposite banks of the Negro and Japurá rivers, which delimit 3 areas of endemism in northern Amazonia: Napo, Jau, and Guiana. Our results demonstrate that the Negro River is a geographical barrier for both Furnaricola sp. and its avian host, Dendrocincla fuliginosa. Phylogenies of both hosts, Dendrocincla fuliginosa, and the parasites, Furnaricola sp., show monophyletic clades on opposite margins of the river that are not sister taxa. These clades have a mean uncorrected p-distance of 17.8% for Furnaricola sp. and 6.0% for Dendrocincla fuliginosa. Thus, these parasite clades constitute distinct evolutionary lineages and may even be distinct species. In contrast, Dixiphia pipra has no population structure associated with either river. Accordingly, data from their lice Myrsidea sp. indicate weak support for different clades on opposite margins of the Negro River, whereas data from their lice Tyranniphilopterus sp. indicate weak structure across the Japurá. This study is a first step toward understanding the effects of biogeographic history on permanent ectoparasites and suggests that host biogeographic history is to some extent a determinant of the parasite's history. Furthermore, the parasite's evolutionary history is an additional source of information about their hosts' evolution in this highly diverse region of northern Amazonia.

Arsenophonus symbiosis with louse flies: multiple origins, coevolutionary dynamics, and metabolic significance.

IMPORTANCE: Insects that live exclusively on vertebrate blood utilize symbiotic bacteria as a source of essential compounds, e.g., B vitamins. In louse flies, the most frequent symbiont originated in genus Arsenophonus, known from a wide range of insects. Here, we analyze genomic traits, phylogenetic origins, and metabolic capacities of 11 Arsenophonus strains associated with louse flies. We show that in louse flies, Arsenophonus established symbiosis in at least four independent events, reaching different stages of symbiogenesis. This allowed for comparative genomic analysis, including convergence of metabolic capacities. The significance of the results is twofold. First, based on a comparison of independently originated Arsenophonus symbioses, it determines the importance of individual B vitamins for the insect host. This expands our theoretical insight into insect-bacteria symbiosis. The second outcome is of methodological significance. We show that the comparative approach reveals artifacts that would be difficult to identify based on a single-genome analysis.

Molecular detection of hemotropic Mycoplasma and Bartonella species in lice from sheep and goats of Mexico.

The knowledge of lice associated with small ruminants, especially sheep and goats, is scarce. In Mexico, there are historical reports of six species of chewing and sucking lice associated with Capra hircus and Ovis canadensis. However, the reports did not analyze the ecology of the infestations or the presence of potentially pathogenic bacteria. For this reason, the objectives of this study were i) to identify the species of lice associated with sheep and goats in three states of the Mexican Republic, ii) to characterize the infestations, and iii) to identify the presence of bacterial pathogens. From October 2019 to August 2021, six ranches with sheep and goats were sampled in the states of Hidalgo and Veracruz. Hosts were visually inspected, and lice were retrieved with forceps. The specimens were sexed and identified using morphological taxonomic keys. DNA extraction was performed individually, and a fragment of the cytochrome oxidase subunit 1 gene (COI) was amplified for the molecular identification of the specimens. Subsequently, Anaplasma, Bartonella, Ehrlichia, Mycoplasma, and Rickettsia were molecularly detected. Additionally, the infestations were characterized by calculating the prevalence and mean abundances. We collected 563 specimens of three species, Bovicola caprae, Bovicola ovis, and Linognathus africanus. The highest infestation levels were recorded for B. ovis (66.7%; 4.4) from Veracruz. Additionally, two Bartonella species were detected: Bartonella mellophagi in B. ovis and Bartonella capreoli in L. africanus. In contrast, Mycoplasma ovis was detected exclusively in one pool of B. ovis. This study provides new bacterial-ectoparasite associations and highlights the possible role of these neglected ectoparasites as vectors in the populations of sheep and goats from Mexico.

Effectiveness of a fixed-dose combination injectable (0.2 mg/kg doramectin + 6.0 mg/kg levamisole hydrochloride) against Rhipicephalus microplus and sucking lice infesting cattle.

Unmanaged tick and sucking lice infestations negatively impact the health and production potential of cattle. Described herein are two non-interference dose confirmation studies evaluating the efficacy of a single administration of a new fixed-dose combination injectable (FDCI) endectocide consisting of 0.2 mg/kg doramectin + 6.0 mg/kg levamisole hydrochloride, against either laboratory-induced Rhipicephalus microplus infestations in Australia or naturally acquired sucking lice (Linognathus vituli) infestations in the US. This FDCI is available as Dectomax V® in Australia and New Zealand and as Valcor® in the United States. To evaluate therapeutic efficacy against R. microplus, 12 calves were each exposed to 10 infestations of ∼5000 larvae per infestation between Days -24 and -2. Calves were either treated on Day 0 with the FDCI or left untreated (control). Additional R. microplus infestations of ∼5000 larvae were conducted on Day 2 and then three times weekly to also evaluate persistent efficacy of the FDCI. Tick collections were conducted daily from Day -3. Group mean live tick counts, egg production, and egg viability were analyzed for significant differences between the two groups. To determine efficacy of the FDCI against lice, 24 cattle with active sucking lice infestations based on Day -7 counts were allocated to two groups and treated on Day 0 with either saline (control) or the FDCI. Lice counts were conducted weekly from Day 14 through 42 and again on Day 56. Mean group lice counts on each count day were compared between treatment groups. In the R. microplus study presented here, cattle in Queensland, Australia treated with the FDCI (Dectomax V®) showed > 90 % reduction in tick counts based on arithmetic means within 48 h of treatment when compared to untreated cattle, and counts were > 95 % reduced from post-treatment Day 5 through Day 30. In the sucking lice study conducted in the US, the FDCI (Valcor®) displayed 100 % efficacy against sucking lice infestations (L. vituli) from first count day (Day 14 post-treatment) through Day 35 and then 99.9 % efficacy through Day 56 post-treatment. No treatment-related adverse events were reported for cattle in either study. Using R. microplus and sucking lice as representative ectoparasites, these studies demonstrate the ectoparasite activity of doramectin is retained in the new FDCI.

PHEROMONE COMMUNICATION IN FEATHER-FEEDING WING LICE (INSECTA: PHTHIRAPTERA).

Pheromone communication is central to the life history of insect parasites. Determining how pheromones affect parasite behavior can provide insights into host-parasite interactions and suggest novel avenues for parasite control. Lice infest thousands of bird and mammal species and feed on the host's feathers or blood. Despite the pervasiveness of lice in wild populations and the costs they exact on livestock and poultry industries, little is known about pheromone communication in this diverse group. Here, we test for pheromone communication in the wing lice (Columbicola columbae) of Rock Doves (Columba livia). Wing lice spend the majority of their lives on bird flight feathers where they hide from host preening by inserting their bodies between coarse feather barbs. To feed, wing lice must migrate to bird body regions where they consume the insulating barbs of contour feathers. We first show that wing lice readily form aggregations on flight feathers. Next, using a Y-tube olfactometer, we demonstrate that wing lice use pheromone communication to move toward groups of nearby conspecifics. This pheromone is likely an aggregation pheromone, as wing lice only produce the pheromone when placed on flight feathers. Finally, we found that when forced to choose between groups of male and female lice, male lice move toward male groups and females toward female groups, suggesting the use of multiple pheromones. Ongoing work aims to determine the chemical identity and function of these pheromones.

Species of Reticulipeurus Kéler, 1958 (Phthiraptera, Ischnocera, Oxylipeurus-complex) parasitic on species of Arborophila, with description of a new subgenus and three new species.

A new subgenus and three new species of parasitic lice (Phthiraptera: Ischnocera) in the Oxylipeurus-complex are described and illustrated. Forcipurellus new subgenus is part of the genus Reticulipeurus Kéler, 1958, and only includes species parasitic on partridges of the genus Arborophila Hodgson, 1837. The new species are: Reticulipeurus (Forcipurellus) diki new species ex Arborophila rubrirostris (Salvadori, 1879); Reticulipeurus (Forcipurellus) bracatus new species ex Arborophila atrogularis (Blyth, 1849); Reticulipeurus (Forcipurellus) longistylus new species ex Arborophila rufogularis guttata Delacour & Jabouille, 1928 and two other host subspecies. In addition, we redescribe the species Reticulipeurus (Forcipurellus) formosanus (Uchida, 1917) ex Arborophila crudigularis (Swinhoe, 1864) and Reticulipeurus (Forcipurellus) nitzschi Kéler, 1958 ex Arborophila torqueola torqueola (Valenciennes, 1825) and Arborophila torqueola millardi (Baker, 1921). Also, we briefly discuss four additional species that could not be described based on the specimens examined. A key to identify all species in the subgenus Forcipurellus is provided, as well as an emendation to include Forcipurellus in a previously published key to the Oxylipeurus-complex.

Molecular pathogen screening of louse flies (Diptera: Hippoboscidae) from domestic and wild ruminants in Austria.

BACKGROUND: Hippoboscid flies (Diptera: Hippoboscidae), also known as louse flies or keds, are obligate blood-sucking ectoparasites of animals, and accidentally of humans. The potential role of hippoboscids as vectors of human and veterinary pathogens is being increasingly investigated, but the presence and distribution of infectious agents in louse flies is still unknown in parts of Europe. Here, we report the use of molecular genetics to detect and characterize vector-borne pathogens in hippoboscid flies infesting domestic and wild animals in Austria. METHODS: Louse flies were collected from naturally infested cattle (n = 25), sheep (n = 3), and red deer (n = 12) across Austria between 2015 and 2019. Individual insects were morphologically identified to species level and subjected to DNA extraction for molecular pathogen screening and barcoding. Genomic DNA from each louse fly was screened for Borrelia spp., Bartonella spp., Trypanosomatida, Anaplasmataceae, Filarioidea and Piroplasmida. Obtained sequences of Trypanosomatida and Bartonella spp. were further characterized by phylogenetic and haplotype networking analyses. RESULTS: A total of 282 hippoboscid flies corresponding to three species were identified: Hippobosca equina (n = 62) collected from cattle, Melophagus ovinus (n = 100) from sheep and Lipoptena cervi (n = 120) from red deer (Cervus elaphus). Molecular screening revealed pathogen DNA in 54.3% of hippoboscids, including infections with single (63.39%), two (30.71%) and up to three (5.90%) distinct pathogens in the same individual. Bartonella DNA was detected in 36.9% of the louse flies. Lipoptena cervi were infected with 10 distinct and previously unreported Bartonella sp. haplotypes, some closely associated with strains of zoonotic potential. DNA of trypanosomatids was identified in 34% of hippoboscids, including the first description of Trypanosoma sp. in H. equina. Anaplasmataceae DNA (Wolbachia spp.) was detected only in M. ovinus (16%), while < 1% of the louse flies were positive for Borrelia spp. and Filarioidea. All hippoboscids were negative for Piroplasmida. CONCLUSIONS: Molecular genetic screening confirmed the presence of several pathogens in hippoboscids infesting domestic and wild ruminants in Austria, including novel pathogen haplotypes of zoonotic potential (e.g. Bartonella spp.) and the first report of Trypanosoma sp. in H. equina, suggesting a potential role of this louse fly as vector of animal trypanosomatids. Experimental transmission studies and expanded monitoring of hippoboscid flies and hippoboscid-associated pathogens are warranted to clarify the competence of these ectoparasites as vectors of infectious agents in a One-Health context.

Characterization of the fragmented mitochondrial genome of domestic pig louse Haematopinus suis (Insecta: Haematopinidae) from China.

The domestic pig louse Haematopinus suis (Linnaeus, 1758) (Phthiraptera: Anoplura) is a common ectoparasite of domestic pigs, which can act as a vector of various infectious disease agents. Despite its significance, the molecular genetics, biology and systematics of H. suis from China have not been studied in detail. In the present study, the entire mitochondrial (mt) genome of H. suis isolate from China was sequenced and compared with that of H. suis isolate from Australia. We identified 37 mt genes located on nine circular mt minichromosomes, 2.9 kb-4.2 kb in size, each containing 2-8 genes and one large non-coding region (NCR) (1,957 bp-2,226 bp). The number of minichromosomes, gene content, and gene order in H. suis isolates from China and Australia are identical. Total sequence identity across coding regions was 96.3% between H. suis isolates from China and Australia. For the 13 protein-coding genes, sequence differences ranged from 2.8%-6.5% consistent nucleotides with amino acids. Our result is H. suis isolates from China and Australia being the same H. suis species. The present study determined the entire mt genome of H. suis from China, providing additional genetic markers for studying the molecular genetics, biology and systematics of domestic pig louse.

Identification and estimation of the prevalence of ectoparasites of backyard chicken in Boloso Sore District, Wolaita zone, southern Ethiopia.

BACKGROUND: Poultry ectoparasites are the basic cause of retarded growth, low liveliness and poor condition of birds directly by inducing irritation, discomfort, tissue damage, blood loss, toxicosis, allergies and dermatitis which in turn reduce the quality and quantities of meat and egg production and indirectly by being mechanical or biological vectors of pathogens. METHOD: A cross-sectional study was conducted from November 2020 to April 2021 to identify and estimate the prevalence of ectoparasites of chicken managed under a backyard system in the selected area of Boloso Sore district of Wolaita zone, southern Ethiopia. A total of 322 chickens of different age groups, breeds and both sexes were selected by simple random technique and examined for ectoparasite. RESULTS: From the total, 56.52%(182/322) of chickens were infested with one or more species of ectoparasites that mainly grouped into fleas 30.34%(98/322), lice 21.7%(70/322) and fowl tick 4.34%(14/322), there by six species of ectoparasites were identified. Among them, Echidnophaga gallinacean (stick tight flea) 30.34%(98/322) was the most prevalent ectoparasite species followed by lice species (Menopon gallinae 11.80% (38/322), Menacanthus stramineous 6.21%(20/322), Goniocotes gigas 2.48% (8/70) and Goniocotes gallinae 1.24%(4/322) while the least identified was fowl tick (Argas persicus) 4.34%(14/322). Age of the chicken was shown statistically significant (p < 0.05) association with the infestation of ectoparasites in which young chicken was found more (72.5%) infested than adults (27.5%). Also, there was a statistically significant (P < 0.05) difference in the prevalence rate of ectoparasites between both sexes of the chicken, in which females (71.4%) was higher than that of male chicken (28.6%). The local breed was found more highly infested (57.1%) than exotic breeds (42.9%) but the difference was not statistically significant (P > 0.05). There was statistically nonsignificant (P > 0.05) flea infestation in adults 34.14% (43/126) than in young 28.06% (55/196), females 31.34% (63/201) than males 20.66% (25/121), and local breed 31.76% (54/170) than exotic breed 28.95% (44/152). Lice prevalence was statistically nonsignificant (p-value >0.05) higher in adults 38.89% (49/126) than young 10.71% (21/196), female 25.87% (52/201) than males 14.88% (18/121) and local breed 24.12% (41/170) than exotic breed 19.08% (29/152). CONCLUSION: Generally, the study indicated that the external parasites were highly prevalent in backyard chickens in the study area, which was associated with a lack of attention to the hygienic management system, treatment and control practices which necessitate the application of integrated prevention and control measures like awareness creation to the community on the overall effect of ectoparasites on the productivity of poultry and prevention methods.

External and gastrointestinal parasites of the black-faced ibis Theristicus melanopis (Pelecaniformes: Threskiornithidae) in the Los Ríos region, southern Chile.

The black-faced ibis, Theristicus melanopis, is considered a useful bird species for agricultural activity because it preys upon various invertebrate and vertebrate pests. Although it is a common species in Chile, limited information is available regarding its parasites. The main objective of this study was to recover the diversity of ectoparasites and gastrointestinal helminths in black-faced ibises living in the communes of Valdivia and Panguipulli, Los Ríos region. A total of 74 specimens were received for examination from the Centro de Rehabilitación de Fauna Silvestre at the Universidad Austral de Chile (CEREFAS-UACh), Valdivia, in 2011-2015. Black-faced ibises were externally inspected for ectoparasites by direct examining of the plumage, and necropsies were performed to examine digestive and respiratory organs in search of endoparasites. For each taxon, prevalence, mean intensity, mean abundance, and range of parasites per bird were estimated. Five species of ectoparasites and six species of helminths were identified. A total of 298 lice (Insecta: Phthiraptera) belonging to four species were collected: Ardeicola melanopis (13.51%), Colpocephalum trispinum (20.27%), Ibidoecus fissisignatus (4.05%), and Plegadiphilus mamillatus (9.46%). In addition, one feather mite species, Diodochaetus melanopis (Acari: Pterolichoidea) (17.56%), was isolated. In 48 black-faced ibis (64.86%), a total of 1229 gastrointestinal helminths were found: two nematodes, Porrocaecum heteropterum (55.41%) and Baruscapillaria obsignata (24.32%); one tapeworm Eugonodaeum nasuta (20.27%); two digeneans, Echinoparyphium recurvatum (1.35%) and Strigea bulbosa (6.76%); and the acanthocephalan Sphaerirostris sp. (1.35%). The findings of the following parasites present new host-parasite associations: P. mamillatus, D. melanopis, B. obsignata, E. recurvatum, S. bulbosa, and Sphaerirostris sp. Additionally, the louse P. mamillatus, feather mite D. melanopis, platyhelminths E. nasuta, E. recurvatum and S. bulbosa, and the acanthocephalan Sphaerirostris sp. are new records for the fauna of Chile.

Ecological factors shaping the ectoparasite community assembly of the Azara's Grass Mouse, Akodon azarae (Rodentia: Cricetidae).

Parasites are integral members of the global biodiversity. They are useful indicators of environmental stress, food web structure and diversity. Ectoparasites have the potential to transmit vector-borne diseases of public health and veterinary importance and to play an important role in the regulation and evolution of host populations. The interlinkages between hosts, parasites and the environment are complex and challenging to study, leading to controversial results. Most previous studies have been focused on one or two parasite groups, while hosts are often co-infected by different taxa. The present study aims to assess the influence of environmental and host traits on the entire ectoparasite community composition of the rodent Akodon azarae. A total of 278 rodents were examined and mites (Mesostigmata), lice (Phthiraptera), ticks (Ixodida) and fleas (Siphonaptera) were determined. A multi-correspondence analysis was performed in order to analyze interactions within the ectoparasite community and the influence of environmental and host variables on this assembly. We found that environmental variables have a stronger influence on the composition of the ectoparasite community of A. azarae than the host variables analyzed. Minimum temperature was the most influential variable among the studied. In addition, we found evidence of agonistic and antagonistic interactions between ticks and mites, lice and fleas. The present study supports the hypothesis that minimum temperature plays a major role in the dynamics that shape the ectoparasite community of A. azarae, probably through both direct and indirect processes. This finding becomes particularly relevant in a climate change scenario.

Molecular investigation on infection by haemosporidians in three Western Palearctic species of swift (Apodidae) and their ectoparasitic louse flies.

Swifts (Apodidae) are an unusual group of birds that spend most of their lives in flight, landing only when breeding. Although this aerial lifestyle greatly reduces their likelihood of being bitten by vectors and infected by vector-born parasites, swifts can still be heavily infested during breeding by nest-based vectors such as louse flies (Hippoboscidae). Here, we investigated host, vector, and vector-borne parasite relationships in the three most widespread swift species in the Western Palearctic (WP): common swifts (Apus apus), pallid swifts (A. pallidus), and alpine swifts (Tachymarptis melba), their nest-based louse flies (Crataerina pallida and C. melbae) and avian haemosporidians (genera Haemoproteus, Plasmodium, and Leucocytozoon). Studies of haemosporidian infections in Apodidae remain limited, with clear evidence of infection found to date in just four Neotropical and one Australasian species. The possible role of louse flies in transmitting haemosporidian infections has never been tested in swifts. We assessed the occurrence of haemosporidian infection by PCR screenings of DNA from blood samples from 34 common swifts and 44 pallid swifts from Italy, and 45 alpine swifts from Switzerland. We also screened 20 ectoparasitic louse flies present on 20 birds and identified them by both morphological features and cytochrome oxidase subunit 1 (COI) barcodes. Our results provide no evidence of haemosporidian infection in the 123 swifts tested or in the two louse fly species we identified. Our findings are consistent with available knowledge showing no haemosporidian occurrence in WP swift species and that the most likely infection route for these highly aerial species (via louse fly ectoparasites during nesting) is unlikely.