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1.
Int J Biol Macromol ; 261(Pt 2): 129932, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38309399

RESUMO

Hydrogels possessing both conductive characteristics and notable antibacterial and antioxidant properties hold considerable significance within the realm of wound healing and recovery. The object of current study is the development of conductive hydrogels with antibacterial and antioxidant properties, emphasizing their potential for effective wound healing, especially in treating third-degree burns. For this purpose, various conductive hydrogels are developed based on tragacanth and silk fibroin, with variable dopamine functionalized carboxyl-capped aniline pentamer (CAP@DA). The FTIR analysis confirms that the CAP powder was successfully synthesized and modified with DA. The results show that the incorporation of CAP@DA into hydrogels can increase the porosity and swellability of the hydrogels. Additionally, the mechanical and viscoelastic properties of the hydrogels are also improved. The release of vancomycin from the hydrogels is sustained over time, and the hydrogels are effective in inhibiting the growth of Methicillin-resistant Staphylococcus aureus (MRSA). In vitro cell studies of the hydrogels show that all hydrogels are biocompatible and support cell attachment. The hydrogels' tissue adhesiveness yielded a satisfactory hemostatic outcome in a rat-liver injury model. The third-degree burn was created on the dorsal back paravertebral region of the rats and then grafted with hydrogels. The burn was monitored for 3, 7, and 14 days to evaluate the efficacy of the hydrogel in promoting wound healing. The hydrogels revealed treatment effect, resulting in enhancements in wound closure, dermal collagen matrix production, new blood formation, and anti-inflammatory properties. Better results were obtained for hydrogel with increasing CAP@DA. In summary, the multifunctional conducive hydrogel, featuring potent antibacterial properties, markedly facilitated the wound regeneration process.


Assuntos
Queimaduras , Fibroínas , Staphylococcus aureus Resistente à Meticilina , Tragacanto , Ratos , Animais , Antioxidantes/farmacologia , Fibroínas/farmacologia , Dopamina/farmacologia , Tragacanto/farmacologia , Hidrogéis/farmacologia , Cicatrização , Queimaduras/tratamento farmacológico , Hemostasia , Antibacterianos/farmacologia
2.
Eur J Pharm Sci ; 192: 106657, 2024 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-38040098

RESUMO

Periodontitis consists a group of dental disorders that affect about 70 % of the world population. The therapy mainly relies on mechanical removing bacterial biofilm, nevertheless, local or systemic antibacterial agents play a key role in treating the acute conditions. Secnidazole is a newer derivative of commonly used metronidazole with high safety profile and broad spectrum of antimicrobial activity. The aim of the study was to evaluate the applicability of polyelectrolyte complex-based hydrogels composed of anionic tragacanth with addition of xanthan gum and cationic chitosan as carriers for buccal/intra pocket delivery of secnidazole. Prepared hydrogels with 5 % and 10 % (w/w) drug content were evaluated pharmaceutically towards inter alia physicomechanical, rheological and thermal properties, drug release kinetics, swelling behavior or antimicrobial activity. Cytotoxicity against human primary umbilical vein endothelial cells was also assessed with two independent method. Stable compositions with secnidazole were obtained, however, various miscibility of the drug with the polymers was noted. By adding chitosan, antibacterial activity and swelling performance of the gels were improved, nevertheless, drop of the mucoadhesiveness was also recorded. Hydrogels with 5 % secnidazole were selected as effective antimicrobial compositions with the highest cytocompatibility. They might be considered as promising for oromucosal application with special attention given to SEC as an alternative locally administered antimicrobial agent.


Assuntos
Quitosana , Tragacanto , Humanos , Metronidazol/farmacologia , Células Endoteliais , Antibacterianos/farmacologia , Hidrogéis
3.
Int J Biol Macromol ; 257(Pt 1): 128343, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38007020

RESUMO

Herein, glutamic acid, lysine, arginine and glycine grafted tragacanth gum (TG) were synthesized and designated as TG-Glu, TG-Lys, TG-Arg, and TG-Gly, respectively. The corresponding degrees of substitution (DS) were 0.212, 0.255, 0.394, and 0.169. Thermal, antioxidant, and antibacterial properties of synthesized amino acid-grafted tragacanth gum (ATG) were investigated. The results suggested that the grafting of amino acids onto TG has the potential to alter its thermal properties. When compared with TG and amino acid alone, ATG exhibited significantly enhanced antioxidant and antibacterial properties, with these properties being concentration-dependent. At a concentration of 2 mg/mL for TG-Glu and 3 mg/mL for TG-Arg, TG-Gly, and TG-Lys, the scavenging rate for 2,2'-hypoazido-3-ethylbenzothiazoline sulfonate (ABTS) radical reached 100 %. On the other hand, the scavenging rate of TG-Glu for hydroxyl radical achieved 100 % even at a concentration as low as 1 mg/mL. These properties were accompanied by an increase in reducing force and a notable improvement in the ability to scavenge superoxide anion (O2-). Moreover, the combination of amino acids and TG represents a promising approach to enhance the antimicrobial activities of TG, with the bacteriostatic rate reaching 100 %. Consequently, ATG shows promise as a novel agent for both antioxidation and antimicrobial applications.


Assuntos
Anti-Infecciosos , Tragacanto , Antioxidantes/farmacologia , Tragacanto/química , Aminoácidos/metabolismo , Anti-Infecciosos/farmacologia , Antibacterianos/farmacologia
4.
Int J Biol Macromol ; 253(Pt 8): 127502, 2023 Dec 31.
Artigo em Inglês | MEDLINE | ID: mdl-37863139

RESUMO

Micronutrient deficiencies, such as iron, folic acid, and vitamins C and D, are currently prevalent due to inadequate consumption of natural food sources, namely raw vegetables and fruits. This deficiency is compounded by the growing reliance on synthetic nutraceuticals and processed food, which exhibit poor absorbency within the gastrointestinal tract. Scientific studies consistently indicate that naturally prepared whole foods are superior in terms of nutrient absorption compared to processed and synthetic supplements. To address this issue, we utilized FDA-approved tragacanth gum (TG) in the synthesis of nano-nutraceuticals by encapsulating beetroot juice and ball-milled sweet basil (Ocimum basilicum). TG, in its micro or macro form, possesses the remarkable ability to form hydrogels capable of absorbing water up to 50 times its weight. However, the hydrogel-forming property diminishes when TG is reduced to the nanoscale. We effectively exploited these properties to facilitate the synthesis of nano-nutraceuticals. The procedure involved encapsulating beetroot juice and sweet basil nanopowder using TG hydrogel, followed by freeze-drying. Subsequently, the freeze-dried encapsulated TG composite was subjected to ball-milling to achieve the desired nano-nutraceuticals. These nano-nutraceuticals naturally contain essential nutrients such as iron, folic acid, ascorbic acid, chlorophyll, niacin, and sugars, without the need for chemical processing or preservatives.


Assuntos
Ocimum basilicum , Oligoelementos , Tragacanto , Micronutrientes , Tragacanto/química , Suplementos Nutricionais , Antioxidantes , Ferro , Hidrogéis
5.
Int J Biol Macromol ; 253(Pt 2): 126481, 2023 Dec 31.
Artigo em Inglês | MEDLINE | ID: mdl-37634769

RESUMO

The present study investigated the effects of composite coatings made of chitosan (CS), tragacanth gum (TG), and polyvinyl alcohol (PVA) containing cinnamon essential oil (CEO) on the shelf-life of refrigerated chicken breast fillets. The samples were treated with different coating dispersions, and coded as: T1 (distilled water as control), T2 (blank composite coating), and T3 (composite coating containing CEO). Results showed that incorporating CEO into CS/TG/PVA coatings could significantly increase the quality of chicken fillets. The obtained results showed that after 21 days, the total microbial population of lactic acid bacteria (LAB), psychrotrophic and mesophilic bacteria in T3 samples was less than T1 and T2 samples. In addition, the highest antioxidant activity (48.04 %) and total phenolic content (TPC) values (2.458 mg gallic acid /g), the best sensory characteristics and the lowest pH (5.73), total volatile basic nitrogen (TVB-N) (21.89 mg N/100 g), thiobarbituric acid reactive substances (TBARS) (1.678 mg malondialdehyde equivalent/kg) and percentage of cooking loss (30 %) were related to T3. Results disclosed that this composite coating is a promising technology to improve the shelf life of chicken fillets during storage.


Assuntos
Quitosana , Óleos Voláteis , Tragacanto , Animais , Armazenamento de Alimentos/métodos , Conservação de Alimentos/métodos , Óleos Voláteis/farmacologia , Quitosana/farmacologia , Quitosana/química , Cinnamomum zeylanicum , Galinhas , Álcool de Polivinil
6.
Int J Biol Macromol ; 249: 126041, 2023 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-37516227

RESUMO

As pivotal role of scaffold in tissue engineering (TE), the aim of present study was to design and development of extracellular matrix (ECM)-mimetic electrically conductive nanofibrous scaffolds composed of polyaniline-grafted tragacanth gum (TG-g-PANI) and poly(vinyl alcohol) (PVA) with different PANI content for skin tissue engineering (STE) application. The fabricated scaffolds were preliminary evaluated in terms of some physicochemical and biological properties. Cytocompatibility and cells proliferation properties of the scaffolds were examined with the well-known MTT assay, and it was found that the developed scaffolds have proper cytocompatibilities and can enhances the mouse fibroblast L929 cells adhesion as well as proliferation, which confirm their potential for STE applications. Hemocompatibility assay revealed that the hemolysis rate of the fabricated scaffolds were <2 % even at a relatively high concentration (200 µgmL-1) of samples, therefore, these scaffolds can be considered as safe. Human serum albumin (HSA) protein adsorption capacities of the fabricated scaffolds were quantified as 42 and 49 µgmg-1 that represent suitable values for a successful TE. Overall, the fabricated scaffold with 20 wt% of TG-g-PANI showed higher potential in both physicochemical and biological features than scaffold with 30 wt% of mentioned copolymer for STE application.


Assuntos
Nanofibras , Tragacanto , Camundongos , Animais , Humanos , Engenharia Tecidual , Álcool de Polivinil/química , Tecidos Suporte/química , Tragacanto/química , Nanofibras/química , Poliésteres/química , Matriz Extracelular
7.
Int J Biol Macromol ; 245: 125225, 2023 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-37285892

RESUMO

The aim of this investigation was to prepare and characterize active composite films made of chitosan (CS), tragacanth gum (TG), polyvinyl alcohol (PVA) and loaded with different concentrations of cinnamon essential oil (CEO) nanoemulsion (CEO, 2 and 4 % v/v). For this purpose, the amount of CS was fixed and the ratio of TG to PVA (90:10, 80:20, 70:30, and 60:40) was considered variable. The physical (thickness and opacity), mechanical, antibacterial and water-resistance properties of the composite films were evaluated. According to the microbial tests, the optimal sample was determined and evaluated with several analytical instruments. CEO loading increased the thickness and EAB of composite films, while decreasing light transmission, tensile strength, and water vapor permeability. All the films containing CEO nanoemulsion had antimicrobial properties, but this activity was higher against Gram-positive bacteria (Bacillus cereus and Staphylococcus aureus) than Gram-negative types (Escherichia coli (O157:H7) and Salmonella typhimurium). According to the results of attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), thermogravimetric analysis (TGA) and X-ray diffraction (XRD), the interaction between the components of the composite film was confirmed. It can be concluded that the CEO nanoemulsion can be incorporated in CS/TG/PVA composite films and successfully used as active and environmentally friendly packaging.


Assuntos
Anti-Infecciosos , Quitosana , Óleos Voláteis , Tragacanto , Óleos Voláteis/farmacologia , Óleos Voláteis/química , Quitosana/química , Cinnamomum zeylanicum/química , Álcool de Polivinil/química , Anti-Infecciosos/farmacologia , Anti-Infecciosos/química , Antibacterianos/farmacologia , Antibacterianos/química , Embalagem de Alimentos/métodos
8.
Curr Drug Deliv ; 20(8): 1163-1175, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37183384

RESUMO

PURPOSE: The goal of the present research was to isolate a biopolymer from Phaseolus vulgaris (P. vulgaris) and Zea mays (Z. mays) plants and used it to construct Resveratrol (RES)-loaded translabial films. METHODS: Biopolymers were extracted from P. vulgaris and Z. mays seeds using a simple process. Separated biopolymers, sodium carboxymethylcellulose (SCMC) and tragacanth were subjected to formulation development by incorporating RES-loaded translabial films. The Fourier-transform infrared spectroscopy (FTIR), physical appearance, weight, thickness, folding endurance, swelling index, surface pH, percent moisture absorption, percent moisture loss, vapor transfer rate, and content uniformity of the translabial films were examined. The mucoadhesive, ex-vivo permeation, in vivo and stability studies, were performed. RESULTS: The results showed that RES-loaded translabial films produced from P. vulgaris and Z. mays biopolymers exhibited exceptional mucoadhesive, stability, and permeation properties. Results revealed that the best formulations were prepared from a combination of biopolymer (P. vulgaris C or Z. mays C) with tragacanth. Formulations with tragacanth revealed good swelling and thus permeation profiles. In vivo release of TL 11 was found to be 24.05 ng/ml in 10 hours and it was stable enough at 45°C. CONCLUSION: This research suggested that RES-loaded translabial formulations can be potentially used for the treatment of Parkinson's disease with good patient compliance to geriatric and unconscious patients.


Assuntos
Doença de Parkinson , Tragacanto , Humanos , Idoso , Preparações Farmacêuticas , Doença de Parkinson/tratamento farmacológico , Sistemas de Liberação de Medicamentos , Excipientes/química
9.
Int J Biol Macromol ; 242(Pt 3): 125100, 2023 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-37236557

RESUMO

Constructing biodegradable food packaging with good mechanics, gas barrier and antibacterial properties to maintain food quality is still challenge. In this work, mussel-inspired bio-interface emerged as a tool for constructing functional multilayer films. Konjac glucomannan (KGM) and tragacanth gum (TG) with physical entangled network are introduced in the core layer. Cationic polypeptide ε-polylysine (ε-PLL) and chitosan (CS) producing cationic-π interaction with adjacent aromatic residues in tannic acid (TA) are introduced in the two-sided outer layer. The triple-layer film mimics the mussel adhesive bio-interface, where cationic residues in outer layers interact with negatively charged TG in the core layer. Furthermore, a series of physical tests showed excellent performance of triple-layer film with great mechanical properties (tensile strength (TS): 21.4 MPa, elongation at break (EAB): 7.9 %), UV-shielding (almost 0 % UV transmittance), thermal stability, water, and oxygen barrier (oxygen permeability (OP): 1.14 × 10-3 g/m s Pa and water vapor permeability (WVP): 2.15 g mm/m2 day kPa). In addition, the triple-layer film demonstrated advanced degradability, antimicrobial functions, and presented good moisture-proof performance for crackers, which can be potentially applied as dry food packaging.


Assuntos
Quitosana , Tragacanto , Embalagem de Alimentos , Quitosana/química , Polilisina/química , Resistência à Tração , Taninos , Permeabilidade , Oxigênio
10.
Int J Biol Macromol ; 241: 124343, 2023 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-37054856

RESUMO

Tragacanth is an abundant natural gum extracted from some plants and is dried for use in various applications from industry to biomedicines. It is a cost-effective and easily accessible polysaccharide with desirable biocompatibility and biodegradability, drawing much attention for use in new biomedical applications such as wound healing and tissue engineering. Moreover, this anionic polysaccharide with a highly branched structure has been used as an emulsifier and thickening agent in pharmaceutical applications. In addition, this gum has been introduced as an appealing biomaterial for producing engineering tools in drug delivery. Furthermore, the biological properties of tragacanth gum have made it a favorable biomaterial in cell therapies, and tissue engineering. This review aims to discuss the recent studies on this natural gum as a potential carrier for different drugs and cells.


Assuntos
Tragacanto , Tragacanto/química , Materiais Biocompatíveis/química , Sistemas de Liberação de Medicamentos , Excipientes , Terapia Baseada em Transplante de Células e Tecidos
11.
Int J Biol Macromol ; 237: 124250, 2023 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-36996955

RESUMO

This study aimed to investigate the segregative interaction of gelatin (G) and tragacanth gum (TG) and the stabilization of their water-in-water (W/W) emulsion by G-TG complex coacervate particles. Segregation was studied at different pHs, ionic strengths and biopolymer concentrations. Results showed that incompatibility was affected by increasing the biopolymer concentrations. So, three reigns were demonstrated in the phase diagram of the salt-free samples. NaCl significantly changed the phase behavior via enhancement of self-association of polysaccharide and changing solvent quality due to the charge screening effect of ions. The W/W emulsion prepared from these two biopolymers and stabilized with G-TG complex particles was stable for at least one week. The microgel particles improved emulsion stability by adsorption to the interface and creating a physical barrier. A fibrous and network-like structure of the G-TG microgels was observed by scanning electron microscopy images suggesting the Mickering emulsion stabilization mechanism. It was confirmed that the bridging flocculation between the microgel polymers led to phase separation after the stability period. Biopolymer incompatibility investigation is a useful tool to obtain beneficial knowledge for preparation new food formulation, especially no contain oil emulsions for low- calorie diets.


Assuntos
Microgéis , Tragacanto , Emulsões/química , Gelatina , Tragacanto/química , Água/química
12.
Int J Biol Macromol ; 235: 123856, 2023 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-36870665

RESUMO

Recent advancements in development of natural polymer nono-composites led to exploration of potential of gum acacia (GA) and tragacanth gum (TG) for design of silver nanoparticles (AgNPs) impregnated grafted copolymers via green approach for use in drug delivery (DD). The formation of copolymers was confirmed by UV-Vis spectroscopy, TEM, SEM, AFM, XPS, XRD, FTIR,TGA and DSC. UV-Vis spectra indicated the formation of AgNPs using GA as reducing agent. TEM, SEM, XPS and XRD revealed impregnation of AgNPs inside the copolymeric network hydrogels. TGA inferred thermal stability of polymer enhanced by grafting and incorporation of AgNPs. The non-Fickian diffusion of antibiotic drug meropenem was revealed from drug encapsulated GA-TG-(AgNPs)-cl-poly(AAm) network which were also pH responsive and release profile was fitted in Korsmeyer-Peppas kinetic model. Sustained release was due to polymer-drug interaction. The polymer-blood interaction demonstrated biocompatible characteristics of polymer. Mucoadhesive property exhibited by copolymers because of supra-molecular interactions. Antimicrobial characteristics were shown by copolymers against bacteria S. flexneri, P. auroginosa, and B. cereus.


Assuntos
Nanopartículas Metálicas , Tragacanto , Nanopartículas Metálicas/química , Prata/química , Sistemas de Liberação de Medicamentos , Polissacarídeos , Antibacterianos/química , Goma Arábica/química
13.
Carbohydr Polym ; 305: 120548, 2023 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-36737197

RESUMO

Nanocomposite hydrogels based on tyramine conjugated gum tragacanth, poly (vinyl alcohol) (PVA), and halloysite nanotubes (HNTs) were prepared by electron beam irradiation and characterized. The FTIR, 1H NMR, and TGA results confirmed the chemical incorporation of HNTs into gum tragacanth. Gel content and swelling of hydrogels decreased with HNTs loading up to 20 % wt. The mechanical strength of hydrogels increased by increasing HNTs content up to 10 % with 371 kPa fracture stress at 0.95 fracture strain, compared to 312 kPa stress at 0.79 strain for gum tragacanth/PVA hydrogel. Hydrogel's biocompatibility and osteogenic activity were tested by seeding rabbit bone marrow mesenchymal stem cells. The cell viability was >85 % after 7 days of culture. In vitro secretion of ALP and calcium deposition on hydrogels in alizarin red assay after 21 days of culture indicated hydrogel potential for bone tissue engineering.


Assuntos
Engenharia Tecidual , Tragacanto , Animais , Coelhos , Argila , Elétrons , Hidrogéis/farmacologia , Hidrogéis/química , Engenharia Tecidual/métodos , Tragacanto/química , Álcool de Polivinil/química
14.
Int J Biol Macromol ; 232: 123292, 2023 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-36652983

RESUMO

This research focuses on preparing a natural-based drug delivery system for α-arbutin (AR) as a skin lightening. Bacterial cellulose nanofibers (BC) pad was used for controlled-AR release through two approaches. First was the dip-drying method (P-BC), in which AR cross-linked to BC pads using citric acid (CA). The second was simultaneously entrapping of AR in Tragacanth gum (AR-TG) and stabilized on BC (BC-T) through the ultrasonic-assisted microemulsion method. UV-Vis spectra revealed better control of AR release in BC-T in the first hour. High cell viability (above 70 %) of the pads containing 1-3 % AR was reported using MTT assay. The in-vitro permeation study indicated the proper AR penetration in the treated pads. The Fickian diffusion model was determined as a fitted model for all pads in the drug release kinetics. FTIR, XRD, and TGA analyses further characterized the pads. FESEM images verified AR-TG and BC structures with average diameters of 410.7 ± 25.4 and 34.5 ± 7.51 nm, respectively. The hydrophilicity and mechanical properties of the pads were also investigated. Finally, the high biocompatibility, initial controlled release, and proper permeation suggested BC-T as a more promising delivery platform for AR.


Assuntos
Nanofibras , Tragacanto , Arbutina , Sistemas de Liberação de Medicamentos , Pele
15.
Int J Biol Macromol ; 231: 123240, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36639083

RESUMO

The mitigation of wastewater exploiting biopolymers/natural gums-based composites is an appealing research theme in today's scenario. The following review presents a comprehensive description of the polysaccharides derived from biopolymers (chitosan, collagen, cellulose, starch, pectin, lignin, and alginate) and natural gums (guar, gellan, carrageenan, karaya, moringa oliefera, tragacanth, and xanthan gum). These biopolymers/natural gums-based composites depicted excellent surface functionality, non-toxicity, economic and environmental viability, which corroborated them as potential candidates in the decontamination process. The presence of -OH, -COOH, and -NH functional groups in their backbone rendered them tailorable for modification/functionalization, and anchor an array of pollutants via electrostatic interaction, hydrogen bonding, and Van der Waals forces. Further, due to these functional moieties, these bio-based composites revealed an excellent adsorption capacity than conventional adsorbents. This review provides an overview of the classification of biopolymers/natural gums based on their origin, different ways of their modification, and the remediation of dye-contaminated aqueous environments employing diverse bio-based adsorbents. The isotherm, kinetic modelling along with thermodynamics of the adsorption process is discussed. Additionally, the reusable efficacy of these bio-adsorbents is reviewed.


Assuntos
Tragacanto , Poluentes Químicos da Água , Celulose , Amido , Alginatos , Pectinas , Adsorção
16.
J Biomater Sci Polym Ed ; 34(2): 217-242, 2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-35960146

RESUMO

In this study, a Gelatin/Tragacanth/Nano-hydroxyapatite scaffold was fabricated via freeze-drying method. A highly porous scaffold with an average pore diameter of 142 µm and porosity of 86% was found by the micro-computed tomography. The mean compressive strength of the scaffold was about 1.5 MPa, a value in the range of the spongy bone. The scaffold lost 10 wt.% of its initial weight after 28 days soaking in PBS that shows a fair degradation rate for a bone tissue engineering scaffold. Apatite formation ability of the scaffold was confirmed via scanning electron microscopy, X-ray diffraction and Fourier transforming infrared spectroscopy, after 28 days soaking in simulated body fluid. The scaffold was able to deliver 93% of the loaded drug, Quercetin, during 120 h in phosphate-buffered solution, in a sustainable manner. The MTT assay using human bone mesenchymal stem cells showed 84% cell viability of the Quercetin-loaded scaffold. The expression of the osteogenic genes including Col I, Runx-2, BGLAP (gene of osteocalcin), bFGF, SP7 (gene of osterix) and SPP1 (gene of osteopontin) were all upregulated when Quercetin was loaded on the scaffold, which indicates the synergetic effect of the drug and the scaffold.


Assuntos
Engenharia Tecidual , Tragacanto , Humanos , Durapatita/química , Quercetina/farmacologia , Gelatina/química , Tragacanto/farmacologia , Microtomografia por Raio-X , Preparações de Ação Retardada/farmacologia , Tecidos Suporte/química , Osteogênese , Porosidade , Expressão Gênica , Proliferação de Células
17.
J Biomater Appl ; 37(8): 1409-1422, 2023 03.
Artigo em Inglês | MEDLINE | ID: mdl-36045495

RESUMO

Management of uncontrolled bleeding due to traumatic injuries occurring in battlefields and road traffic accidents is a major healthcare concern, especially in developing countries like India. Since natural coagulation mechanism alone is insufficient to achieve haemostasis quickly in such cases, application of an external haemostatic product is generally required to accelerate the coagulation process. We had recently reported preliminary comparison of four natural absorbent gums, which indicated towards haemostatic potential of gum tragacanth (GT) and xanthan gum (XG). Present study involves fabrication of haemostatic dressings incorporated with different concentrations of GT or XG, along with ciprofloxacin (a broad-spectrum antibiotic) and other excipients over woven cotton gauze. Prepared gauzes were investigated for physico-chemical characteristics, in-vitro blood interaction studies, antibacterial effect and in-vivo haemostatic efficacy in Sprague Dawley rats using two bleeding models. Acute dermal toxicity studies were also carried out as per OECD guidelines. SEM studies showed that gauzes coated with XG had thin, uniform layer of coating, while in case of GT; coating was comparatively rough with insoluble particles of GT adhering over gauze surface, forming voids on the fibers. Coated gauzes exhibited optimum mechanical properties in terms of tensile strength and percent extension at break. GT coated dressings showed good fluid uptake and retention ability in-vitro. Test gauzes were non-hemolytic in nature, did not elicit any dermal toxicity on animals' skin and had the ability to protect against E. coli infection. In-vivo efficacy studies in rat femoral artery and liver laceration bleeding models indicated that gauzes coated with 4% GT were able to clot blood in least time (36.67 ± 3.33s and 40 ± 2.58s respectively) as compared to other gum combinations and commercially available dressing 'Surgispon® (103.3 ± 4.22s and 85 ± 5.62s respectively). Results of this study validate our initial findings of the potential of gum tragacanth to be developed into a suitable haemostatic product.


Assuntos
Hemostáticos , Tragacanto , Ratos , Animais , Tragacanto/química , Escherichia coli , Ratos Sprague-Dawley , Hemostáticos/farmacologia , Hemostáticos/uso terapêutico , Hemorragia/tratamento farmacológico , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Antibacterianos/química , Bandagens
18.
Int J Biol Macromol ; 226: 1411-1425, 2023 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-36442552

RESUMO

Hybridization of nanomaterials (NMs) with natural polymers is one of the best techniques to promote their exciting properties. In this way, the main objective of this work was to investigate the efficiency of decoration of the graphene oxide (GO) nano-sheets with tragacanth gum (TG) polysaccharide. To aim this, different approaches were used (with and without ultrasonic treatment) and various tests (XRD, FTIR, Raman, UV-Vis, DLS, Zeta potential, contact angle, AFM, FE-SEM, TEM, and MTT assay) were conducted. Test results indicated that the nano-hybrids were successfully synthesized. Furthermore, our findings represented that, the TG hybridized GO (TG-GO) appreciably enhanced the biocompatibility of GO. Moreover, it was demonstrated that the ultrasonic treatment of TG solution put a remarkable impact on the microstructure, wettability, and also surface charge characteristic of fabricated nano-hybrids and consequently improved the biocompatibility against L929-fibroblast cells.


Assuntos
Grafite , Nanoestruturas , Tragacanto , Tragacanto/química , Grafite/farmacologia , Grafite/química , Polímeros
19.
Methods Mol Biol ; 2587: 45-53, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36401023

RESUMO

Histological and molecular genetic evaluation of skeletal and cardiac muscles is an indispensable part of understanding muscle biology and the pathology of muscle disorders. Proper processing of the muscle tissue is a prerequisite for optimal evaluation. However, the processing of skeletal muscle samples often comes with many challenges. One of the commonly used methods of frozen tissue preparation involves optimal cutting temperature compound (OCT compound) embedding. This method is considered optimal for the processing of most of the routinely studied tissue samples. However, the processing of skeletal muscle samples using this method is often unsuitable as it causes artifacts and low DNA, RNA, and protein yield and quality due to the slow freezing of skeletal muscle tissues that allows ice crystals to form. One of the most suitable methods for skeletal muscle tissue processing for histological, genetic, and molecular studies is rapid freezing of freshly collected tissue samples using isopentane cooled with liquid nitrogen and tragacanth gum, which provides distinct advantages in consuming less time, preserving the cell morphology, and helping higher nucleic acids and protein yields. This chapter describes a protocol for rapid freezing of freshly collected skeletal muscle tissues using isopentane pre-chilled with liquid nitrogen and tragacanth gum. Skeletal muscle tissue samples processed using this protocol can be used for histological and immunological staining investigations and studies requiring DNA, RNA, and proteins from these tissues.


Assuntos
Tragacanto , Congelamento , Pentanos , Músculo Esquelético/fisiologia , Miocárdio , Nitrogênio , RNA
20.
Biofouling ; 38(10): 965-983, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36519335

RESUMO

Biomaterial associated bacterial infections are indomitable to treatment due to the rise in antibiotic resistant strains, thereby triggering the need for new antibacterial agents. Herein, composite bactericidal hydrogels were formulated by incorporating silver nanotriangles (AgNTs) inside a hybrid polymer network of Gum Tragacanth/Sodium Alginate (GT/SA) hydrogels. Physico-chemical examination revealed robust mechanical strength, appreciable porosity and desirable in vitro enzymatic biodegradation of composite hydrogels. The antibacterial activity of AgNT-hydrogel was tested against planktonic and biofilm-forming Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria. For all the strains, AgNT-hydrogel showed a dose-dependent decrease in bacterial growth. The addition of AgNT-hydrogels (40-80 mg ml-1) caused 87% inhibition of planktonic biomass and up to 74% reduction in biofilm formation. Overall, this study proposes a promising approach for designing antibacterial composite hydrogels to mitigate various forms of bacterial infection.


Assuntos
Infecções Estafilocócicas , Tragacanto , Humanos , Prata/farmacologia , Prata/química , Hidrogéis/farmacologia , Hidrogéis/química , Tragacanto/química , Alginatos/farmacologia , Biofilmes , Bactérias , Escherichia coli , Antibacterianos/farmacologia , Antibacterianos/química
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