Chapter 9: Life as We Don't Know It.

While Earth contains the only known example of life in the universe, it is possible that life elsewhere is fundamentally different from what we are familiar with. There is an increased recognition in the astrobiology community that the search for life should steer away from terran-specific biosignatures to those that are more inclusive to all life-forms. To start exploring the space of possibilities that life could occupy, we can try to dissociate life from the chemistry that composes it on Earth by envisioning how different life elsewhere could be in composition, lifestyle, medium, and form, and by exploring how the general principles that govern living systems on Earth might be found in different forms and environments across the Solar System. Exotic life-forms could exist on Mars or Venus, or icy moons like Europa and Enceladus, or even as a shadow biosphere on Earth. New perspectives on agnostic biosignature detection have also begun to emerge, allowing for a broader and more inclusive approach to seeking exotic life with unknown chemistry that is distinct from life as we know it on Earth.

Chapter 3: The Origins and Evolution of Planetary Systems.

The materials that form the diverse chemicals and structures on Earth-from mountains to oceans and biological organisms-all originated in a universe dominated by hydrogen and helium. Over billions of years, the composition and structure of the galaxies and stars evolved, and the elements of life, CHONPS, were formed through nucleosynthesis in stellar cores. Climactic events such as supernovae and stellar collisions produced heavier elements and spread them throughout the cosmos, often to be incorporated into new, more metal-rich stars. Stars typically form in molecular clouds containing small amounts of dust through the collapse of a high-density core. The surrounding nebular material is then pulled into a protoplanetary disk, from which planets, moons, asteroids, and comets eventually accrete. During the accretion of planetary systems, turbulent mixing can expose matter to a variety of different thermal and radiative environments. Chemical and physical changes in planetary system materials occur before and throughout the process of accretion, though many factors such as distance from the star, impact history, and level of heating experienced combine to ultimately determine the final geophysical characteristics. In Earth's planetary system, called the Solar System, after the orbits of the planets had settled into their current configuration, large impacts became rare, and the composition of and relative positions of objects became largely fixed. Further evolution of the respective chemical and physical environments of the planets-geosphere, hydrosphere, and atmosphere-then became dependent on their local geochemistry, their atmospheric interactions with solar radiation, and smaller asteroid impacts. On Earth, the presence of land, air, and water, along with an abundance of important geophysical and geochemical phenomena, led to a habitable planet where conditions were right for life to thrive.

Chapter 7: Assessing Habitability Beyond Earth.

All known life on Earth inhabits environments that maintain conditions between certain extremes of temperature, chemical composition, energy availability, and so on (Chapter 6). Life may have emerged in similar environments elsewhere in the Solar System and beyond. The ongoing search for life elsewhere mainly focuses on those environments most likely to support life, now or in the past-that is, potentially habitable environments. Discussion of habitability is necessarily based on what we know about life on Earth, as it is our only example. This chapter gives an overview of the known and presumed requirements for life on Earth and discusses how these requirements can be used to assess the potential habitability of planetary bodies across the Solar System and beyond. We first consider the chemical requirements of life and potential feedback effects that the presence of life can have on habitable conditions, and then the planetary, stellar, and temporal requirements for habitability. We then review the state of knowledge on the potential habitability of bodies across the Solar System and exoplanets, with a particular focus on Mars, Venus, Europa, and Enceladus. While reviewing the case for the potential habitability of each body, we summarize the most prominent and impactful studies that have informed the perspective on where habitable environments are likely to be found.

Organic Input to Titan's Subsurface Ocean Through Impact Cratering.

Titan has an organic-rich atmosphere and surface with a subsurface liquid water ocean that may represent a habitable environment. In this work, we determined the amount of organic material that can be delivered from Titan's surface to its ocean through impact cratering. We assumed that Titan's craters produce impact melt deposits composed of liquid water that can founder in its lower-density ice crust and estimated the amount of organic molecules that could be incorporated into these melt lenses. We used known yields for HCN and Titan haze hydrolysis to determine the amount of glycine produced in the melt lenses and found a range of possible flux rates of glycine from the surface to the subsurface ocean. These ranged from 0 to 1011 mol/Gyr for HCN hydrolysis and from 0 to 1014 mol/Gyr for haze hydrolysis. These fluxes suggest an upper limit for biomass productivity of ∼103 kgC/year from a glycine fermentation metabolism. This upper limit is significantly less than recent estimates of the hypothetical biomass production supported by Enceladus's subsurface ocean. Unless biologically available compounds can be sourced from Titan's interior, or be delivered from the surface by other mechanisms, our calculations suggest that even the most organic-rich ocean world in the Solar System may not be able to support a large biosphere.

Field-Based Planetary Protection Operations for Melt Probes: Validation of Clean Access into the Blood Falls, Antarctica, Englacial Ecosystem.

Subglacial environments on Earth offer important analogs to Ocean World targets in our solar system. These unique microbial ecosystems remain understudied due to the challenges of access through thick glacial ice (tens to hundreds of meters). Additionally, sub-ice collections must be conducted in a clean manner to ensure sample integrity for downstream microbiological and geochemical analyses. We describe the field-based cleaning of a melt probe that was used to collect brine samples from within a glacier conduit at Blood Falls, Antarctica, for geomicrobiological studies. We used a thermoelectric melting probe called the IceMole that was designed to be minimally invasive in that the logistical requirements in support of drilling operations were small and the probe could be cleaned, even in a remote field setting, so as to minimize potential contamination. In our study, the exterior bioburden on the IceMole was reduced to levels measured in most clean rooms, and below that of the ice surrounding our sampling target. Potential microbial contaminants were identified during the cleaning process; however, very few were detected in the final englacial sample collected with the IceMole and were present in extremely low abundances (∼0.063% of 16S rRNA gene amplicon sequences). This cleaning protocol can help minimize contamination when working in remote field locations, support microbiological sampling of terrestrial subglacial environments using melting probes, and help inform planetary protection challenges for Ocean World analog mission concepts.

A Comparative Study of Methods for Detecting Extraterrestrial Life in Exploration Missions to Mars and the Solar System II: Targeted Characteristics, Detection Techniques, and Their Combination for Survey, Detection, and Analysis.

We present a comparative study of the methods used in the search for extraterrestrial microorganism life, including a summary table where different life-detection techniques can be easily compared as an aid to mission and instrument design aimed at life detection. This is an extension of previous study, where detection techniques for a series of target characteristics and molecules that could constitute a positive life detection were evaluated. This comparison has been extended with a particular consideration to sources of false positives, the causes of negative detection, the results of detection techniques when presented regarding terrestrial life, and additional science objectives that could be achieved outside the primary aim of detecting life. These additions address both the scientific and programmatic side of exploration mission design, where a successful proposal must demonstrate probable outcomes and be able to return valuable results even if no life is found. The applicability of the life detection techniques is considered for Earth life, Earth-independent life (life emerging independently from that on Earth,) and Earth-kin life (sharing a common ancestor with life on Earth), and techniques effective in detecting Earth life should also be useful in the detection of Earth-kin life. However, their applicability is not guaranteed for Earth-independent life. As found in our previous study, there exists no realistic single detection method that can conclusively determine the discovery of extraterrestrial life, and no method is superior to all others. In this study, we further consider combinations of detection techniques and identify imaging as a valuable addition to molecule detection methods, even in cases where there is insufficient resolution to observe the detailed morphology of a microbial cell. The search for extraterrestrial life is further divided into a survey-and-detection and analysis-and-conclusion step. These steps benefit from different detection techniques, but imaging is necessary for both parts.

Abundant presolar grains and primordial organics preserved in carbon-rich exogenous clasts in asteroid Ryugu.

Preliminary analyses of asteroid Ryugu samples show kinship to aqueously altered CI (Ivuna-type) chondrites, suggesting similar origins. We report identification of C-rich, particularly primitive clasts in Ryugu samples that contain preserved presolar silicate grains and exceptional abundances of presolar SiC and isotopically anomalous organic matter. The high presolar silicate abundance (104 ppm) indicates that the clast escaped extensive alteration. The 5 to 10 times higher abundances of presolar SiC (~235 ppm), N-rich organic matter, organics with N isotopic anomalies (1.2%), and organics with C isotopic anomalies (0.2%) in the primitive clasts compared to bulk Ryugu suggest that the clasts formed in a unique part of the protoplanetary disk enriched in presolar materials. These clasts likely represent previously unsampled outer solar system material that accreted onto Ryugu after aqueous alteration ceased, consistent with Ryugu's rubble pile origin.

Confidence of Life Detection: The Problem of Unconceived Alternatives.

Potential biosignatures that offer the promise of extraterrestrial life (past or present) are to be expected in the coming years and decades, whether from within our own solar system, from an exoplanet atmosphere, or otherwise. With each such potential biosignature, the degree of our uncertainty will be the first question asked. Have we really identified extraterrestrial life? How sure are we? This paper considers the problem of unconceived alternative explanations. We stress that articulating our uncertainty requires an assessment of the extent to which we have explored the relevant possibility space. It is argued that, for most conceivable potential biosignatures, we currently have not explored the relevant possibility space very thoroughly at all. Not only does this severely limit the circumstances in which we could reasonably be confident in our detection of extraterrestrial life, it also poses a significant challenge to any attempt to quantify our degree of uncertainty. The discussion leads us to the following recommendation: when it comes specifically to an extraterrestrial life-detection claim, the astrobiology community should follow the uncertainty assessment approach adopted by the Intergovernmental Panel on Climate Change (IPCC).

Multiobjective optimization of a hybrid electricity generation system based on waste energy of internal combustion engine and solar system for sustainable environment.

In recent years, the interest in generating power through hybrid power generation systems has increased. In this study, a hybrid power generation system including an internal combustion engine (ICE) and a solar system based on flat plate collectors to generate electricity is investigated. To benefit from the thermal energy absorbed by solar collectors, an organic Rankine cycle (ORC) is considered. In addition to the solar energy absorbed by the collectors, the heat source of the ORC is the wasted heat through exhaust gases and the cooling system of the ICE. A two-pressure configuration for ORC is proposed for optimal heat absorption from the three available heat sources. The proposed system is installed to produce power with a capacity of 10 kW. A bi-objective function optimization process is carried out to design this system. The objective of the optimization process is to minimize the total cost rate and maximize the exergy efficiency of the system. The design variables of the present problem include the ICE rated power, the number of solar flat plate collectors (SFPC), the pressure of the high-pressure (HP) and low-pressure (LP) stage of the ORC, the degree of superheating of the HP and LP stage of the ORC, and its condenser pressure. Finally, it is observed among the design variables the most impact on total cost and exergy efficiency is related to the ICE rated power and the number of SFPCs.

Carnobacterium Species Capable of Growth at Pressures Ranging Over 5 Orders of Magnitude, from the Surface of Mars (103 Pa) to Deep Oceans (107 Pa) in the Solar System.

Several permanently cold solar system bodies are being investigated with regard to their potential habitability, including Mars and icy moons. In such locations, microbial life would have to cope with low temperatures and both high and low pressures, ranging from ∼102 to 103 Pa on the surface of Mars to upward of ∼108-109 Pa in the subsurface oceans of icy moons. The bacterial genus Carnobacterium consists of species that were previously shown to be capable of growth in the absence of oxygen at low temperatures and at either low pressure or high pressure, but to date the entire pressure range of the genus has not been explored. In the present study, we subjected 14 Carnobacterium strains representing 11 species to cultivation in a complex liquid medium under anaerobic conditions at 2°C and at a range of pressures spanning 5 orders of magnitude, from 103 to 107 Pa. Eleven of the 14 strains showed measurable growth rates at all pressures tested, representing the first demonstration of terrestrial life forms capable of growth under such a wide range of pressures. These findings expand the physical boundaries of the capabilities of life to occur in extreme extraterrestrial environments.

Potassium isotope heterogeneity in the early Solar System controlled by extensive evaporation and partial recondensation.

Volatiles are vital ingredients for a habitable planet. Angrite meteorites sample the most volatile-depleted planetesimal in the Solar System, particularly for the alkali elements. They are prime targets for investigating the formation of volatile-poor rocky planets, yet their exceptionally low volatile content presents a major analytical challenge. Here, we leverage improved sensitivity and precision of K isotopic analysis to constrain the mechanism of extreme K depletion (>99.8%) in angrites. In contrast with the isotopically heavy Moon and Vesta, we find that angrites are strikingly depleted in the heavier K isotopes, which is best explained by partial recondensation of vaporized K following extensive evaporation on the angrite parent body (APB) during magma-ocean stage. Therefore, the APB may provide a rare example of isotope fractionation controlled by condensation, rather than evaporation, at a planetary scale. Furthermore, nebula-wide K isotopic variations primarily reflect volatility-driven fractionations instead of presolar nucleosynthetic heterogeneity proposed previously.

'Oumuamua Is Not a Probe Sent to Our Solar System by an Alien Civilization.

'Oumuamua, the first known object of extrasolar origin seen to enter our Solar System, has multiple unusual characteristics that, taken together, are very difficult to explain with conventional astronomical entities like asteroids and comets. Consequently, it has been hypothesized that 'Oumuamua is an interstellar probe that was constructed by an alien civilization. We demonstrate that the accomplishments that can be achieved with large space telescopes/interferometers in the alien's planetary system will completely quench any motivation for construction and launch of an 'Oumuamua-like probe. The absence of any such motivation attests that 'Oumuamua is not an alien creation. The existence of large space telescopes has important implications for a range of topics that include interstellar space travel, the Zoo Hypothesis, METI, and UFOs.

Origin of life-forming volatile elements in the inner Solar System.

Volatile elements such as hydrogen, carbon, nitrogen and oxygen are essential ingredients to build habitable worlds like Earth, but their origin and evolution on terrestrial planets remain highly debated. Here we discuss the processes that distributed these elements throughout the early Solar System and how they then became incorporated into planetary building blocks. Volatiles on Earth and the other terrestrial planets appear to have been heterogeneously sourced from different Solar System reservoirs. The sources of planetary volatiles and the timing at which they were accreted to growing planets probably play a crucial role in controlling planet habitability.

From Materials to Devices: Rationally Designing Solar Steam System for Advanced Applications.

Solar-driven water vaporization for freshwater production attracts significant interest due to its potential for solving global water scarcity problems. In this review, the recent development of management strategies via diverse rational designs in terms of light, thermal, water, and anti-salt fouling for enhancement of overall vaporization efficiency, is summarized. For device design, a host-guest concept is raised for clearly elaborating the detailed function and interaction between the solar-thermal material and the substrates. In addition, the rising technologies derived from solar vaporization, such as energy generation, photocatalysis, dehumidification, salt harvesting, sterilization, and biofuel production, are also highlighted. This review provides a new horizon toward the development of solar technologies and practical applications.

Comparative study of methods for detecting extraterrestrial life in exploration mission of Mars and the solar system.

The detection and analysis of extraterrestrial life are important issues of space science. Mars is among the most important planets to explore for extraterrestrial life, owing both to its physical properties and to its ancient and present environments as revealed by previous exploration missions. In this paper, we present a comparative study of methods for detecting extraterrestrial life and life-related substances. To this end, we have classified and summarized the characteristics targeted for the detection of extraterrestrial life in solar system exploration mission and the methods used to evaluate them. A summary table is presented. We conclude that at this moment (i) there is no realistic single detection method capable of concluding the discovery of extraterrestrial life, (ii) no single method has an advantage over the others in all respects, and (iii) there is no single method capable of distinguishing extraterrestrial life from terrestrial life. Therefore, a combination of complementary methods is essential. We emphasize the importance of endeavoring to detect extraterrestrial life without overlooking possible alien life forms, even at the cost of tolerating false positives. Summaries of both the targets and the detection methods should be updated continuously, and comparative studies of both should be pursued. Although this study assumes Mars to be a model site for the primary environment for life searches, both the targets and detection methods described herein will also be useful for searching for extraterrestrial life in any celestial environment and for the initial inspection of returned samples.

Urability: A Property of Planetary Bodies That Can Support an Origin of Life.

The concept of habitability is now widely used to describe zones in a solar system in which planets with liquid water can sustain life. Because habitability does not explicitly incorporate the origin of life, this article proposes a new word-urability-which refers to the conditions that allow life to begin. The utility of the word is tested by applying it to combinations of multiple geophysical and geochemical factors that support plausible localized zones that are conducive to the chemical reactions and molecular assembly processes required for the origin of life. The concept of urable worlds, planetary bodies that can sustain an arising of life, is considered for bodies in our own solar system and exoplanets beyond.