Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 56
Filtrar
Mais filtros










Filtros aplicados
Base de dados
Intervalo de ano de publicação
1.
Arthritis Rheum ; 38(5): 690-8, May 1995.
Artigo em Inglês | MedCarib | ID: med-2077

RESUMO

OBJECTIVE: To investigate a possible association between human T cell leukemia/lymphoma virus type I (HTLV-I) and polymyositis (PM). METHODS: Sera and muscle biopsy samples from 9 Jamaican PM patients were compared with specimens from American HTLV-I positive PM patients and normal controls. Sera were evaluated for HTLV antibodies by enzyme-linked immunosorbent assay and Western blot. The biopsy samples were analyzed for HTLV-I/II DNA by polymerase chain reaction and were also immunohistochemically stained for HTLV gp46 envelope protein. RESULTS: Seven of the 8 Jamaican PM patients from whom sera were available were HTLV-I seropositive. The muscle biopsies of all 9 Jamaican patients demonstrated severe lymphocytic infiltration, cellular degeneration, myofiber atrophy, and fibrosis. Each muscle biopsy specimen contained HTLV-I DNA. Two of 6 samples demonstrated intense staining for HTLV-I gp46 in many of the invading mononuclear cells and weak staining for HTLV-I gp46 in many of the other specimens were weakly positive for gp46 in rare mononuclear cells. All controls specimens were negative for the presence of HTLV-I DNA and protein. CONClUSION: HTLV-I is associated with an inflammatory muscle disease characterized by direct invasion of the affected muscle by HTLV-I-infected mononuclear cells.(AU)


Assuntos
Adulto , Pessoa de Meia-Idade , DNA Viral/isolamento & purificação , Produtos do Gene env/análise , Anticorpos Anti-HTLV-I/sangue , Polimiosite/virologia , Proteínas Oncogênicas de Retroviridae/análise , Sequência de Bases , Biópsia , Dados de Sequência Molecular , Músculos/química , Músculos/patologia , Reação em Cadeia da Polimerase , Polimiosite/sangue , Polimiosite/imunologia , Polimiosite/patologia
2.
West Indian med. j ; 39(Suppl. 1): 41, April 1990.
Artigo em Inglês | MedCarib | ID: med-5277

RESUMO

During recovery from severe malnutrition, muscle gain is slow relative to overall weight gain. We postulated that this might be due to an inadequate supply of zinc. We therefore compared nitrogen (N) balance and protein synthesis rates (as estimates of muscle gain) during early, mid and late recovery in children fed 3 different levels of dietary zinc (Low Zn gp.n = 4; Moderate Zn gp.n = 4; High Zn gp.n = 3). N intake was similar in the 3 Zn groups throughout recovery. N net absorption and N retention were also similar in early recovery. Later, both increased in the moderate and high Zn groups only: so also did the N retention /g wt gained. By late recovery, they were each significantly higher in the high Zn gp than in the low Zn gp. Thus, Zn supplementation of the relatively low Zn recovery diet resulted in synthesis of tissue richer in N - and therefore, by implication, having a higher proportion of muscle. Protein synthesis rates were calculated from the 15N enrichments of urinary urea and ammonia at plateau, obtained during a constant nasogastric infusion of 15N labeled glycine. Overall, the results using the 2 different end products were not different. However, there was a difference among the Zn gps in the 15N ammonia enrichments. From these values, protein synthesis rates were significantly higher than in the high Zn gp than in the low Zn gp by late recovery (table included). These data are consistent with the N balance data in that they indicate increased muscle protein metabolism with Zn supplementation (AU)


Assuntos
Humanos , Distúrbios Nutricionais , Músculos/crescimento & desenvolvimento , Zinco , Inibidores de Ciclo-Oxigenase , Trinidad e Tobago
3.
Pediatr Pathol ; 10(5): 729-42, 1990.
Artigo em Inglês | MedCarib | ID: med-12253

RESUMO

Babies with major malformations were identified during the Jamaica Perinatal Morbidity and Mortality Survey. They were found in 96 (8.6 percent) of 1112 perinatal and neonatal deaths coming to necropsy and in 28 (2.6 percent) of 1085 not so examined. The central nervous system was most commonly affected, followed by the renal, gastrointestinal, and cardiovascular systems in decreasing order of frequency. Many infants had abnormalities in more than one system and 10 malformations syndromes/sequences were identified. Although at the present time, major malformations make only a small contribution to perinatal and neonatal mortality in Jamaica, their importance will increase when deaths from other causes, such as birth asphyxia, decline. The type of malformation currently fatal in a particular population is relevant when planning diagnostic and surgical services for neonates and infants. It is also important to any discussions about provision of prenatal diagnostic services. (AU)


Assuntos
Humanos , Recém-Nascido , Anormalidades Congênitas/mortalidade , Morte Fetal , Mortalidade Infantil , Anormalidades Congênitas/embriologia , Anormalidades Múltiplas/mortalidade , Vasos Sanguíneos/anormalidades , Osso e Ossos/anormalidades , Sistema Nervoso Central/anormalidades , /anormalidades , Cardiopatias Congênitas/mortalidade , Jamaica , Rim/anormalidades , Pulmão/anormalidades , Músculos/anormalidades , Sistema Urinário/anormalidades
4.
Lancet ; 2(8673): 1184-7, Nov. 18, 1989.
Artigo em Inglês | MedCarib | ID: med-10037

RESUMO

1gG antibodies to human T-cell ilymphotropic virus (HTLV-1) were found in 11 of 13 (85 percent) Jamaican patients with idiopathic adult patients with polymyositis. The association was first observed in 7 patients with polymyositis who were included in a control group of 100 patiants with neurological and neuromusclar diseasee in a serological investigation of the prevalence of HTLV-1 antibody in patients with tropical spastic paraparesis. All 7 patients with polymyositis were positive for the antibody by an enzyme-linked immunosorbent assay, confirmed by western blot. Because of this striking association a further 6 patients with polymyositis were identified and tested, 4 of whom were also seropositive for HTLV-1 antibody. (AU)


Assuntos
Humanos , Adulto , Pessoa de Meia-Idade , Idoso , Masculino , Feminino , Anticorpos Anti-HTLV-I/análise , Infecções por HTLV-I/complicações , Imunoglobulina G/análise , Miosite/complicações , Biópsia , Infecções por HTLV-I/imunologia , Jamaica , Músculos/patologia , Miosite/epidemiologia , Miosite/etiologia , Miosite/imunologia , Miosite/patologia , Fatores Sexuais
5.
Comp Biochem Physiol ; 90C(2): 295-303, 1988.
Artigo em Inglês | MedCarib | ID: med-10181

RESUMO

The surface morphology and organization of the neuromuscular system in Cercaria caribbea LXXI are investigated using scanning and transmission electron microscopy, respectively. Direct and indirect (sarconeural) nerve-muscle junctions are identified in the cercarial tail. Synaptic vesicles at the direct neuromuscular junction suggest cholinergic and aminoacidergic transmission. Those at the sarconeural junctions suggest catecholaminergic and/or serotonergic transmission. Acetylcholinesterase and non-specific cholinesterases were found in high concentrations in the proximal and distal tail. Flourescence histochemistry showed primary catecholamines and 5-HT in the body and tail. 5-HT in the tail may represent a slowly depleted store, perhaps associated with energy availability and utilization. (AU)


Assuntos
Parasitos/ultraestrutura , Junção Neuromuscular/fisiologia , Microscopia Eletrônica , Microscopia Eletrônica de Varredura , Colinesterases , Aminas Biogênicas , Músculos/anatomia & histologia , Serotonina/isolamento & purificação , Catecolaminas/isolamento & purificação , Fluorescência
6.
Anal Biochem ; 121(2): 349-55, Apr. 1982.
Artigo em Inglês | MedCarib | ID: med-13013

RESUMO

The amide nitrogen from L-glutamine has been isolated from an artificial plasma, in a form suitable for mass spectrometry, by a macromodification of the glutaminase reaction. The prior removal of free ammonia was carried out by alkaline aeration. When this was performed at 0§C for 3 h, spontaneous hydrolysis of glutamine was 1.4 percent. Cross-contamination with nitrogen liberated from the amide group of asparagine can be avoided by preincubation with asparaginase for 2h and removal of the freed ammonia prior to reacting with glutaminase. Hydrolysis of glutamine during this step is 12 percent. Measurements of enrichment can be made on samples yielding more than 1 æmol of glutamine amide-derived ammonia.(AU)


Assuntos
Ratos , 21003 , Amidas/isolamento & purificação , Glutamina , Nitrogênio/isolamento & purificação , Química Encefálica , Glutamina/sangue , Rim/análise , Intestinos/análise , Fígado/análise , Músculos/análise , Espectrometria de Massas
7.
Br Med Bull ; 37(1): 5-10, Jan. 1981.
Artigo em Inglês | MedCarib | ID: med-15788

RESUMO

The composition and homoeostasis of the body are maintained by the integrated action of all the tissues and organs. Therefore, although it is of interest to look at the component parts separately, each part has to be fitted functionally into a greater over-all framework. Whole-body protein turnover represents the integration of one fundamental activity of living organisms. Each individual protein has its own turnover rate, responding in a particular way to any change in metabolism. The turnover in the whole body is the resultant of these activities and in this sense has been likened to basal metabolic rate. The measurement of protein turnover in man has wide application, not only to the normal metabolic changes taking place during growth but also to disorders of metabolism in many clinical conditions. Although the standard nitrogen balance technique gives valuable information on net changes, it cannot indicate either the intensity of nitrogen metabolism or the mechanism by which changes are brought about. Nitrogen balance is the resultant of differences between synthesis and breakdown, which may change in the same or opposite directions in any given clinical state.(AU)


Assuntos
Humanos , Lactente , Adulto , Idoso , 21003 , Masculino , Ciências da Nutrição , Proteínas/metabolismo , Fatores Etários , Proteínas na Dieta/metabolismo , Metabolismo Energético , Esforço Físico , Alimentos , Crescimento , Fígado/metabolismo , Músculos/metabolismo , Distúrbios Nutricionais/metabolismo , Proteínas/análise , Proteínas/biossíntese
8.
Br J Nutr ; 39(3): 647-58, May 1978.
Artigo em Inglês | MedCarib | ID: med-13089

RESUMO

The objective of this study was to determine whether the chemical and biochemical changes induced by muscle wasting caused by dietary protein restriction are different in various skeletal muscles. Rats were fasted for 3 d and then fed on a 10 g protein/kg diet for 21d. Thirteen muscles from the trunk, forelimb, and hind-limb regions were analysed for muscle weight, and the content of water, fat, cellular and extracellular protein, DNA and RNA. Results were compared to values for an `initial' control group killed at the start of the experiment. Weight loss was greatest in trunk muscles and least in the distal forelimb muscles. Water content decreased in most muscles, but increased in three forelimb muscles. A significant loss of lipid was found in the gastroenemius, while the biceps brachii gained lipid. Changes in lipid content of the muscles did not form a distinctive pattern. All muscles except the distal forelimb muscles lost a significant amount of cellular protein, while all muscles except the diaphragm gained extracellular protein. DNA content was unchanged in all muscles. The value for cellular protein: DNA was significantly reduced in the rectus abdominis and the diaphragm. A significant loss of RNA was found in all muscles; the percentage change was greatest in trunk muscle and least in the distal forelimb muscles. The values for RNA: protein and RNA: DNA were significantly lower in all muscles except two distal forelimb muscles. With the exception of the water and lipid content of the muscles, the directions of the changes in the experimental animals were the same for all muscles. The results suggested, however, that the magnitude of changes in certain chemical and biochemical indices of composition may depend to some extent on the anatomical location of the muscle: trunk muscles tended to show the greatest percentage change, while the distal forelimbs changed the least.(AU)


Assuntos
21003 , Masculino , Músculos/metabolismo , Deficiência de Proteína/metabolismo , Proteínas na Dieta/administração & dosagem , DNA/análise , Lipídeos/análise , Músculos/análise , Tamanho do Órgão , Proteínas/análise , RNA/análise , Água/análise
9.
Pediatr Res ; 12(3): 167-70, Mar. 1978.
Artigo em Inglês | MedCarib | ID: med-10407

RESUMO

Muscle tissue obtained by needle biopsy from the quadriceps femoris of eight malnourished and eight recovered children were examined histologically. In both groups about two-thirds of the total nuclear count was due to muscle nuclei. The remaining one-third was made up of vascular, nerve, and interstitial cells and isolated nuclei in similar proportions in the two groups. A significantly greater percentage of muscle nuclei consisted of myogenic cells in recovered children as compared to malnourished children(20.7 ñ 6.4 vs. 2.6 ñ 1.4 percent)(AU)


Assuntos
Humanos , Lactente , Pré-Escolar , Masculino , Feminino , Núcleo Celular/ultraestrutura , Desnutrição Proteico-Calórica/patologia , Vasos Sanguíneos/ultraestrutura , DNA/análise , Músculos/análise , Fibras Nervosas/ultraestrutura , Desnutrição Proteico-Calórica/metabolismo
10.
Pediatr Res ; 12: 613-8, 1978.
Artigo em Inglês | MedCarib | ID: med-12061

RESUMO

A method of measuring muscle mass in children with [15N]creatinine has been used to study changes in muscle after recovery from protein-energy malnutrition. Creatinine pool size, muscle mass, total muscle cell number, muscle cell size, and total body water have been measured in seven malnourished and eight recovered children. After recovery there was a significant reduction in the muscle concentration (micrograms mg[-1] wet wt muscle) of creatine (4.21 to 3.12), and a trend towards reduction in noncollagen protein (155 to 136) and DNA (2.13 to 1.34). The fractional turnover rate of creatine did not change but the creatine pool size increased significantly (4.2 to 5.6 g). Average muscle mass almost doubled (1.00 to 1.91 kg) and made up a greater percentage of body weight (16 to 22 percent). When muscle mass was expressed as a percentage of the expected muscle mass for a normal child of the same height the increase with recovery was from 49 percent to 92 percent. Total muscle noncollagen protein (NCP) increased after recovery (153 to 265 g) and accounted for a greater percentage of total body solids (6.6 to 8.5 percent). The average total muscle DNA was 2.049 g in the malnourished and 2.380 g in the recovered children and the ratio of NCP:DNA increased from 92 to 110 on recovery. Total body water as a percentage of body weight was not significantly different after recovery. Values for muscle mass in recovered children were similar to those reported for normal children of the same weight, height, and age. (Summary)


Assuntos
Humanos , Lactente , Pré-Escolar , Composição Corporal , Músculos , Desnutrição Proteico-Calórica , Estatura , Água Corporal , Peso Corporal , Creatinina/análise , Músculos/análise
11.
Am J Clin Nutr ; 30(9): 1514-7, Sept. 1977.
Artigo em Inglês | MedCarib | ID: med-10645

RESUMO

Five children with protein-energy malnutrition were treated with a high calorie, high protein diet and the energy cost of growth in body weight and muscle mass were calculated. Energy expenditures correlated statistically with increases in muscle mass, estimated by [15N] creatine kinetics, but not with gains in body weight.(AU)


Assuntos
Humanos , Lactente , Pré-Escolar , Metabolismo Energético , Crescimento , Desnutrição Proteico-Calórica/metabolismo , Composição Corporal , Ingestão de Energia , Creatina/metabolismo , Músculos/crescimento & desenvolvimento , Músculos/metabolismo , Desnutrição Proteico-Calórica/dietoterapia
13.
Br J Surg ; 64(2): 129-33, Feb. 1977.
Artigo em Inglês | MedCarib | ID: med-12441

RESUMO

Whole body protein turnover was measured in 11 patients before and after elective orthopaedic operations by giving 15N-glycine orally every 4 hours for 32 hours. The patients were maintained throughout on a constant protein intake. In two control subjects a comparison was made between intermittent dosage and continuous infusion of 15N-glycine for the estimation of total turnover. With intermittent dosage the 15N abundance in urinary urea reached a constant level after about 24 hours. Rates of total protein synthesis and breakdown were calculated from the 15N abundance at the plateau level. After surgery there was a moderate increase in urinary N output. The apparent N balance (intake - urinary N) was -0.25 ñ 1.31 g/d (mean ñ s.d.) before operation and =7.51 ñ 4.5g/d/ after operation. The rate of protein synthesis fell from 3.83 ñ 0.73g kg-1d-1 before operation to 2.94 ñ 0.83 g kg-1d-1 after operation. This difference is statistically significant (0.05 > p > 0.01). There was no significant change in the rate of protein breakdown. The possibility remains that a block in protein synthesis, probably mainly in muscle, may be partly responsible for the so-called 'catabolic' loss of nitrogen after injury, but this has not been proved. (AU)


Assuntos
Humanos , Adulto , Pessoa de Meia-Idade , Idoso , Masculino , Feminino , Ortopedia , Proteínas/metabolismo , Artrodese , Fêmur/cirurgia , Articulação do Quadril/cirurgia , Prótese Articular , Músculos/metabolismo , Nitrogênio/metabolismo , Osteotomia , Ureia/urina
15.
Kingston; s.n; Nov. 1976. 81 p. tab.
Tese em Inglês | MedCarib | ID: med-13635

RESUMO

Information derived from a sample of a particular muscle or the entire muscle is often assumed to be characteristic of skeletal muscle in general. There has been little experimental work designed to validate this assumption which has important implications in clinical situations such as severe protein-energy malnutrition in which gross muscle wasting is a characteristic feature. This project was designed to gain information on the changes that take place in a number of muscles in the rat exhibiting severe muscle wasting following dietary deprivation. Eight male albino rats aged 5 weeks and weighing 100g were starved for 33 days and then fed a 1 percent protein diet for 20-22 days. Control animals were albino male rats of the same initial age and body weight. The 13 muscles examined were those of the trunk (rectus abdominis, diaphragm and psoas), thigh (gluteus), hind-limb (quadriceps, tibialis anterior, gastrocnemius and soleus), and fore-limb (Triceps, biceps, brachialis, Flexor carpi radialis and Extensor carpi ulnaris). Estimations on the whole muscle were made of wet weight, dry weight, fat free dry weight, cellular protein, extracellular protein, DNA and RNA. The results showed that the muscles of the experimental animals underwent similar compositional changes in response to the dietary restriction. The changes of four small fore-limb muscle were not characteristic at times. Overall the muscles lost 19 percent wet weight, 17 percent dry weight and 18 percent fat free dry weight. The total water content decreased by approximately 2 percent, cellular protein by 20 percent and total muscle RNA by 44 percent. Extracellular protein increased by 35 percent. There was no significant difference in the total DNA and fat content of nearly all the muscles of control and experimental animals. Although the muscles responded similarly to the dietary treatment the extent of change varied. Caution must be exercised when extrapolating these changes to the entire musculature (Summary)


Assuntos
Ratos , Músculos , Privação de Alimentos , Redução de Peso , Composição Corporal
16.
Pediatr Res ; 10(3): 184-8, Mar. 1976.
Artigo em Inglês | MedCarib | ID: med-10404

RESUMO

In eight infants and children who had recovered from protein-energy malnutrition (PEM), muscle mass was estimated by measuring creatine turnover by an isotope dilution technique using [15N] creatine, creatine concentration, and urinary creatinine output. Creatine turnover varied from 1.5 to 2.6 percent of the muscle creatine pool per day and muscle creatine concentration ranged from 1.7 to 3.9æg/æg muscle DNA. Muscle mass was between 15 percent and 37percent of total body weight. The results indicate that daily creatinine output is not reliable indicator of muscle mass in children who have recently recovered from severe PEM.(AU)


Assuntos
Humanos , Lactente , Pré-Escolar , Creatina/metabolismo , Músculos/anatomia & histologia , Distúrbios Nutricionais/diagnóstico , Composição Corporal/efeitos dos fármacos , Creatina/urina , Técnicas de Diluição do Indicador , Músculos/metabolismo
17.
Acta Paediatr Scand ; 63(6): 817-25, Nov. 1974.
Artigo em Inglês | MedCarib | ID: med-13041

RESUMO

Fasting metabolic rate was investigated in 53 malnourished children and 17 controls of similar age. Total body potassium (TBK) was also measured in 18 of the malnourished children and in all the controls, so that metabolic rates could be compared in relation to a measure of metabolically active tissue. In newly admitted malnourished children specific potassium depletion was corrected orally while they were given a maintenance diet. Resting metabolism correlated better with TBK than with weight, height or surface area in control and recovered children, but metabolic rate per unit TBK decline with increasing body-weight (r=-0.51). This negative correlation became insignificant if metabolic rate was expressed in terms of TBK3/4. In the malnourished children resting metabolic rate was reduced compared with control and recovered values, when expressed in terms of weight, height or surface area. The results were confirmed by a comparison of metabolic rates per unit TBK3/4, which showed a reduction of about 27 percent in the malnourished children. No significant difference was found between children with marasmus and those with oedematous malnutrition. During rapid growth fasting metabolism was increased. We conclude that oxygen consumption in metabolically active tissues is reduced in all forms of untreated infantile malnutrition. (AU)


Assuntos
Humanos , Lactente , Pré-Escolar , Masculino , Feminino , Metabolismo Basal , Kwashiorkor/metabolismo , Potássio/metabolismo , Desnutrição Proteico-Calórica/metabolismo , Composição Corporal , Estatura , Peso Corporal , Dióxido de Carbono , Proteínas na Dieta/uso terapêutico , Kwashiorkor/dietoterapia , Músculos/metabolismo , Consumo de Oxigênio , Desnutrição Proteico-Calórica/dietoterapia
18.
Br J Nutr ; 32(2): 189, 1974.
Artigo em Inglês | MedCarib | ID: med-5059

RESUMO

Total lysosmal hydrolase activities were measured in liver, gastrocnemius muscle and plasma of malnourished and normal rats between 3 and 8 weeks of age. Concurrently, the DNA and protein contents of the livers and muscles were determined. Increased amounts of acid hydrolase activities were found to be associated with subnormal protein: DNA ratios in the tissues of malnourished rats. It was concluded that lysosmal enzymes may be involved in protein catabolism during malnutrition (AU)


Assuntos
21003 , Ratos , Distúrbios Nutricionais , Lisossomos , Fosfatase Ácida , Deficiência de Proteína , Catepsinas , Peso Corporal , Fígado , Músculos
19.
Kingston; s.n; July 1974. 200 p. tab.
Tese em Inglês | MedCarib | ID: med-13659

RESUMO

Measurement of ribonucleases activity in rat diaphragm muscle formed the central part of the work described in this thesis. Two main forms of ribonucleases activity at physiological pH were demonstrated, as characterised by cation dependence and inhibition by ribonuclease inhibitor. These two forms are believed to be alkaline ribonuclease I and alkaline ribonuclease IV previously characterised in liver. Rat diaphragm contained much less alkaline ribonuclease IV activity than liver and kidney. No effect on alkaline ribonuclease IV activity by insulin, fasting, actinomycin D, phrenic denervation, or cycloheximide was observed. Insulin did not have any effect on alkaline ribonuclease I activity, nor on ribonuclease inhibitor activity. Partially purified inhibitor from liver and skeletal muscle stimulated the incorporation of 14C-leucine by heaptic ribosomes. However, this stimulation in amino acid incorporation by the inhibitor seems to be unrelated to the stimulation of incorporation of amino acids caused by insulin (AU)


Assuntos
Ratos , Ribonucleases/metabolismo , Fígado/enzimologia , Músculos , Insulina/metabolismo , Ribonucleases/antagonistas & inibidores
20.
Br J Nutr ; 31(2): 259-70, Mar., 1974.
Artigo em Inglês | MedCarib | ID: med-10402

RESUMO

1. The catabolism of valine was estimated in vivo by measurement of the production of labelled CO2 for 2 h after the oral administration of either [U-14C] valine. It was also estimated in vitro in homogenates of liver and muscle incubated with labelled valine. Experiments were performed in rats given diets providing either 215 g (HP) or 25 g (LP) protein per kg diet. 2. The proportion of [U-14C] valine excreted as 14 CO2 was not reduced in rats given the LP diet for 16 d but the excretion of 14 CO 2 from [1-14C] valine was reduced by 40 percent in these animals. When rats were transferred from the HP diet to the LP diet there was a reduction in the excretion of 14CO2 from [1-14C] valine; when the diet was changed from LP to HP output of 14CO2 increased to control values. 3. Homogenates of muscle and liver catabolized valine to CO2. Both liver and muscle from rats fed on the LP diet catabolized less [1-14C] valine than tissues from control animals. 4. Valine aminotransferase activity was higher in muscle than in liver, and did not change in tissues from rats fed on the LP diet. In these animals 2-ketoisovaleric acid dehydrogenase activity was reduced in both liver and muscle. 5. The production of 14CO2 was lower with [U-14C] valine as the substrate than with [1-14C]-valine and there was no difference between tissues from rats fed on the HP and LP diets. 6. The results with [1-14C] valine suggest that both liver and muscle from protein-depleted rats catabolize valine at a reduced rate. The reason for the discrepancy between these results and those with [U-14C] valine is not clear. It is concluded that the results with [U-14C] valine in vitro are affected by dilution of the label before the formation of 14CO2, but that this does not hold in vivo (AU)


Assuntos
Ratos , 21003 , Valina/metabolismo , Distúrbios Nutricionais/metabolismo , Administração Oral , Alanina Transaminase , Peso Corporal , Dióxido de Carbono/metabolismo , Radioisótopos de Carbono , Proteínas na Dieta , Crescimento/efeitos dos fármacos , Fígado/metabolismo , Músculos/metabolismo , Oxirredutases , Desmame , Técnicas In Vitro
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...