RESUMO
Total lysosmal hydrolase activities were measured in liver, gastrocnemius muscle and plasma of malnourished and normal rats between 3 and 8 weeks of age. Concurrently, the DNA and protein contents of the livers and muscles were determined. Increased amounts of acid hydrolase activities were found to be associated with subnormal protein: DNA ratios in the tissues of malnourished rats. It was concluded that lysosmal enzymes may be involved in protein catabolism during malnutrition (AU)
Assuntos
21003 , Ratos , Distúrbios Nutricionais , Lisossomos , Fosfatase Ácida , Deficiência de Proteína , Catepsinas , Peso Corporal , Fígado , MúsculosAssuntos
Cricetinae , Camundongos , 21003 , Aminas/sangue , Reações Antígeno-Anticorpo , Fibroblastos/imunologia , Soros Imunes , Células L/imunologia , Lisossomos/imunologia , Técnicas de Cultura , Absorção , Contagem de Células , Linhagem Celular , Sobrevivência Celular , Proteínas do Sistema Complemento , Reações Cruzadas , Meios de Cultura , Histocitoquímica , Rim , Microscopia Eletrônica , Polímeros , Espermina , Fatores de TempoRESUMO
Hypoglycin is a toxic amino acid found in the unripe ackee fruit. The ackee is a popular item of diet in Jamaica and has been proposed as a cause of the so-called vomiting sickness. Hypoglycin is thought to act by inhibiting the oxidation of long-chain fatty acids, uncoupling oxidative phosphorylation, and interfering with gluconeogenesis. Hypoglycin was given intraperitoneally to rats in a dose of 10 mg/100 g, and samples of liver taken at hourly intervals up to 5 hr were studied with the electron microscope and compared with controls. The major ultrastructural findings in the hypoglycin-treated rats were progressive mitochondrial swelling with loss of granules and pallor of the matrix, followed by incorporation into autophagic vacuoles. These findings correlate well with the reported biochemical mechanisms.(AU)