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1.
Int J Cancer ; 80(3): 339-44, Jan. 29, 1999.
Artigo em Inglês | MedCarib | ID: med-1409

RESUMO

Human papillomavirus (HPV) is widely accepted as the primary etiologic agent in the development of cervical cancer. DNA of a particular HPV type, HPV 16, is found in about half of tumors tested. Inconsistent with this causal relationship, however, population-based studies of HPV DNA prevalence have often failed to find high rates of anogenital HPV infection in countries with high cervical cancer rates. To examine this issue, we used serology to compare HPV 16 exposure in healthy volunteer blood donors in the United States (n = 278) and similar subjects from a country with 3-fold higher cervical cancer rates, Jamaica (n = 257). Jamaican sexually transmitted disease (STD) patients (n = 831) were also studied to examine in detail the relation of HPV 16 antibodies with sexual history. Serology was conducted using an ELISA employing HPV 16 virus-like particles (VLPs). Age-adjusted seroprevalence rates were greatest among male (29 percent) and female (42 percent) STD patients, intermediate in male (19 percent) and female (24 percent) Jamaican blood donors and lowest among male (3 percent) and female (12 percent) U.S. blood donors. The higher seroprevalence in women was significant, and prevalence tended to increase with age. In multivariate logistic regression, controlling for age and gender, Jamaican blood donors were 4.2-fold (95 percent CI 2.4 - 7.2) and STD patients 8.1-fold (95 percent CI 5.0 - 13.2) more likely to have HPV 16 VLP antibodies than U.S. blood donors. Among STD patients, HPV 16 antibodies were associated with lifetime number of sex partners and years of sexual activity, as well as other factors. Our data suggest that HPV 16 VLP antibodies are strongly associated with sexual behavior. Moreover, exposure to HPV 16 appears to be much greater in Jamaica than in the United States, consistent with the high rate of cervical cancer in Jamaica (Au)


Assuntos
Adulto , Idoso , Adolescente , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudo Comparativo , Anticorpos Antivirais/sangue , Doadores de Sangue/estatística & dados numéricos , Neoplasias do Colo do Útero/virologia , Proteínas Oncogênicas Virais/imunologia , Papillomavirus Humano/imunologia , Fatores Etários , Análise de Variância , Jamaica/epidemiologia , /epidemiologia , /imunologia , Fatores de Risco , Comportamento Sexual , Fatores Sexuais , Parceiros Sexuais , Infecções Sexualmente Transmissíveis/imunologia , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/imunologia , Estados Unidos/epidemiologia , Neoplasias do Colo do Útero , Proteínas Oncogênicas Virais/sangue
2.
J Gen Virol ; 75(9): 2457-62, Sept. 1994.
Artigo em Inglês | MedCarib | ID: med-5903

RESUMO

The naturally occurring sequence variation of human papillomavirus type 16 (HPV-16) was analysed by direct sequence analysis of the PCR products of the long control region (LCR), the E5 and E7 open reading frames (OFRs), a segment of the L2 ORF overlapping the early viral poly(A) signal and a small segment of the L1 ORF or clinical isolates from Barbados and The Netherlands. Despite the widely different geographical and ethnic origin of the two groups of specimens, sequence analysis revealed relatively few mutational differences. Analysis of the LCR and the E5 ORF appeared to be the minimum requirement for the correct positioning of these variants in the HPV-16 phylogenetic tree. Most of the Barbadian variants appeared to be located at a unique position in the HPV-16 phylogenetic tree, at the internal branch close to the point where the European and Asian branches diverge. In contrast, most of the Dutch samples were located on the European branch. (AU)


Assuntos
Humanos , Filogenia , Reação em Cadeia da Polimerase/métodos , /genética , /genética , /isolamento & purificação , Ásia , Barbados , Sequência de Bases , DNA , Etnicidade , Europa (Continente) , Dados de Sequência Molecular , Mutação , Países Baixos , Proteínas Oncogênicas Virais/genética , Estudo Comparativo
3.
J Gen Virol ; 75(Pt 4): 911-16, Apr. 1994.
Artigo em Inglês | MedCarib | ID: med-9377

RESUMO

We have cloned and sequenced the L1 and L2 genes from human papillomavirus type 16 (HPV16) DNA-containing cervical cytology samples collected from the U.K. and Trinidad. Samples containing high copy numbers of HPV16 DNA were selected as being likely to contain fully functional virus DNA molecules in an episomal state, rather than in an integrated and possibly altered state. In comparison with the perviously published sequence of HPV16 isolated from an invasive cancer a variety of differences were detected in both L1 and L2. The pattern of changes appears to be different in samples from the two geographic regions. One of the differences (resulting in D at position 202 of the L1 protein) reported recently to be functionally important for virus particle assembly was found to occur in all the samples examined. Variations in L1 found within known immunoreactive regions or hydrophobic domains should be taken into account in design of prophylactic vaccines for HPV16 based on virus-like particles. All variations within L2 protein were found in hydrophilic domains in the carboxy-terminal half of L2. These positions were highly variable among other types of papillomavirus and are located outside the known L2 immunoreactive region. (AU)


Assuntos
Humanos , Feminino , Capsídeo/genética , Alphapapillomavirus/genética , /microbiologia , Infecções Tumorais por Vírus/microbiologia , Variação Genética/genética , Aminoácidos/análise , Capsídeo/síntese química , Displasia do Colo do Útero/microbiologia , Neoplasias do Colo do Útero/microbiologia , Clonagem Molecular , Sequência Consenso/genética , DNA Viral/isolamento & purificação , Genes Virais , Reino Unido , Proteínas Oncogênicas Virais/síntese química , /genética , Mutação Puntual/genética , Análise de Sequência de DNA , Trinidad e Tobago
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