RESUMO
We have cloned and sequenced the L1 and L2 genes from human papillomavirus type 16 (HPV16) DNA-containing cervical cytology samples collected from the U.K. and Trinidad. Samples containing high copy numbers of HPV16 DNA were selected as being likely to contain fully functional virus DNA molecules in an episomal state, rather than in an integrated and possibly altered state. In comparison with the perviously published sequence of HPV16 isolated from an invasive cancer a variety of differences were detected in both L1 and L2. The pattern of changes appears to be different in samples from the two geographic regions. One of the differences (resulting in D at position 202 of the L1 protein) reported recently to be functionally important for virus particle assembly was found to occur in all the samples examined. Variations in L1 found within known immunoreactive regions or hydrophobic domains should be taken into account in design of prophylactic vaccines for HPV16 based on virus-like particles. All variations within L2 protein were found in hydrophilic domains in the carboxy-terminal half of L2. These positions were highly variable among other types of papillomavirus and are located outside the known L2 immunoreactive region. (AU)
Assuntos
Humanos , Feminino , Capsídeo/genética , Alphapapillomavirus/genética , /microbiologia , Infecções Tumorais por Vírus/microbiologia , Variação Genética/genética , Aminoácidos/análise , Capsídeo/síntese química , Displasia do Colo do Útero/microbiologia , Neoplasias do Colo do Útero/microbiologia , Clonagem Molecular , Sequência Consenso/genética , DNA Viral/isolamento & purificação , Genes Virais , Reino Unido , Proteínas Oncogênicas Virais/síntese química , /genética , Mutação Puntual/genética , Análise de Sequência de DNA , Trinidad e TobagoRESUMO
We report the first complete nucleotide sequence of an adult T cell leukaemia virus/human T cell leukaemia virus type I (ATLV/HTLV) isolate from a British patient of Caribbean origin. Sequence comparisons of our proviral clone (HS-35) with other molecular clones are shown. We note the strong sequence conservation between isolates of Caribbean and Japanese origin (2.3 percent divergence), but demonstrate the higher homologies existing between isolates originating from similar geographical areas (approximately 1 percent divergence). Implications for the origin, evolution and dissemination of the ATLV/HTLV-I subgroup are discussed. Analysis of defective proviral clones isolated from the same genomic library is also reported,and suggests a pattern of proviral sequence deletions during the biogenesis of defective proviruses. (AU)
Assuntos
Humanos , Genes Virais , Infecções por Deltaretrovirus/microbiologia , Deltaretrovirus/genética , Sequência de Aminoácidos , Sequência de Bases , Inglaterra , Infecções por Deltaretrovirus/etnologia , Deltaretrovirus/classificação , Deltaretrovirus/isolamento & purificação , Dados de Sequência Molecular , Filogenia , Provírus/genética , Homologia de Sequência do Ácido Nucleico , Proteínas do Envelope Viral/genética , Índias OcidentaisRESUMO
A rapid nucleic acid hybridization procedure was developed for examining the genotypic variation of dengue type 2 viruses (DEN 2) having distinct RNase T1 fingerprints and isolated from different geographical areas. Synthetic DNA hybridization probes were constructed complementary in nucleotide sequence to common and unique RNase T1 oligonecleotides of topotype viruses from Puerto Rico/South Pacific, Jamaica, the Seychelles, Thailand/Burma and Africa. Hybridization probes with both type- and topotype-specific reactivities were observed, as were probes specific for two or more of the DEN 2 topotypes. These results confirm geographical movement of topotype virus strains and suggest possible origins. Detection of DEN 2 RNA by hybridization is a rapid and reproducible method that can be modified and applied as a viable alternative to the labourious T1 oligonucleotide fingerprinting.(AU)
