RESUMO
The source (species of origin), type, and subgroup (where applicable) of the immunoglobulin chain are indicated prior to the numbers of the first and last amino acid residues comprising the synthesized sequence (stated in parentheses), e.g., human y1 (289-298) or rat & (143-147
Square brackets indicate that the peptide is an analogue, substituted amino acids are identified by the three-letter code; superscripted numbers indicating the residue at which the substitution, removal or addition occurs. Further substitions are specified within round brackets. For example, human y1 (289-298) [D-Thr298] and human y1 (289-298) [Asn (CHO)297](AU)
Assuntos
Peptídeos/induzido quimicamente , Sequência de Aminoácidos , Cadeias Pesadas de Imunoglobulinas , Cadeias Leves de ImunoglobulinaRESUMO
In the last few years, a number of different recombinant and synthetic peptides consisting of the repetitive sequence of the Plasmodum falciparum circumsporozoite protein (NANP)n have been produced and used to develope immunoassays for the detection of antibodies against P. falciparum sporozoites in human sera. A comparative study of three enzyme-linked immunosorbent assays (ELISAs) that employed different (NANP)n peptides (the synthetic peptides (NANP)3 and (NANP)40 as well as the recombinant peptides R32tet32 and R32LR) as carried out using serum samples from individuals who were compared with those obtained for an immunofluorescence assay (IFA) that used glutaraldehyde-fixed sporozoites. All the methods tested exhibited 100 percent specificity on sera from persons not exposed to malaria, good reproducibility (coefficients of variation ranged from 3 percent to 15 percent for peptide-based ELISAs), and good sensitivity. Reproducibility and sensitivity were lower for the IFA than for the peptide-based ELISAs, perhaps because of the subjective element in the interpretation of the results which is inherent in the IFA method. ELISAs based on peptides that contain a higher number of (NANP) repeats, i.e., (NANP)40 and R32tet32 or R32LR, gave results which correlated better with each other than with those obtained with ...(AU)
