Thioflavin T as an Efficient G-Quadruplex Inducer for the Highly Sensitive Detection of Thrombin Using a New Föster Resonance Energy Transfer System.
Liu, Xingfen; Hua, Xiaoxiao; Fan, Quli; Chao, Jie; Su, Shao; Huang, Yan-Qin; Wang, Lianhui; Huang, Wei.
ACS Appl Mater Interfaces
; 7(30): 16458-65, 2015 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-26173915
A straightforward modification in the thrombin binding aptamer improving the stability, affinity to thrombin and nuclease resistance.
Novel application of fluorescence coupled capillary electrophoresis to resolve the interaction between the G-quadruplex aptamer and thrombin.
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Different duplex/quadruplex junctions determine the properties of anti-thrombin aptamers with mixed folding.
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