Your browser doesn't support javascript.
loading
Influence of different types of pulp treatment during isolation in the obtention of human dental pulp stem cells
Viña-Almunia, Jose; Borras, Consuelo; Gambini, Juan; El Alamy, Marya; Peñarrocha, Miguel; Viña, Jose.
Affiliation
  • Viña-Almunia, Jose; University of Valencia. Faculty of Medicine and Dentistry. Stomatology Department. Valencia. Spain
  • Borras, Consuelo; University of Valencia. Faculty of Medicine and Dentistry. Physiology Department. Valencia. Spain
  • Gambini, Juan; University of Valencia. Faculty of Medicine and Dentistry. Physiology Department. Valencia. Spain
  • El Alamy, Marya; University of Valencia. Faculty of Medicine and Dentistry. Physiology Department. Valencia. Spain
  • Peñarrocha, Miguel; University of Valencia. Faculty of Medicine and Dentistry. Stomatology Department. Valencia. Spain
  • Viña, Jose; University of Valencia. Faculty of Medicine and Dentistry. Physiology Department. Valencia. Spain
Med. oral patol. oral cir. bucal (Internet) ; 21(3): e374-e379, mayo 2016. ilus, tab
Article in English | IBECS | ID: ibc-152718
Responsible library: ES1.1
Localization: BNCS
ABSTRACT

BACKGROUND:

Different methods have been used in order to isolate dental pulp stem cells. The aim of this study was to study the effect of different types of pulp treatment during isolation, under 3% O2 conditions, in the time needed and the efficacy for obtaining dental pulp stem cells. MATERIAL AND

METHODS:

One hundred and twenty dental pulps were used to isolate dental pulp stem cells treating the pulp tissue during isolation using 9 different methods, using digestive, disgregation, or mechanical agents, or combining them. The cells were positive for CD133, Oct4, Nestin, Stro-1, CD34 markers, and negative for the hematopoietic cell marker CD-45, thus confirming the presence of mesenchymal stem cells. The efficacy of dental pulp stem cells obtention and the minimum time needed to obtain such cells comparing the 9 different methods was analyzed.

RESULTS:

Dental pulp stem cells were obtained from 97 of the 120 pulps used in the study, i.e. 80.8% of the cases. They were obtained with all the methods used except with mechanical fragmentation of the pulp, where no enzymatic digestion was performed. The minimum time needed to isolate dental pulp stem cells was 8 hours, digesting with 2mg/ml EDTA for 10 minutes, 4mg/ml of type I collagenase, 4mg/ml of type II dispase for 40 minutes, 13ng/ ml of thermolysine for 40 minutes and sonicating the culture for one minute.

CONCLUSIONS:

Dental pulp stem cells were obtained in 97 cases from a series of 120 pulps. The time for obtaining dental pulp stem cells was reduced maximally, without compromising the obtention of the cells, by combining digestive, disgregation, and mechanical agents
Subject(s)

Full text: Available Collection: National databases / Spain Database: IBECS Main subject: Stem Cells / Dental Pulp Type of study: Observational study Limits: Humans Language: English Journal: Med. oral patol. oral cir. bucal (Internet) Year: 2016 Document type: Article Institution/Affiliation country: University of Valencia/Spain

Full text: Available Collection: National databases / Spain Database: IBECS Main subject: Stem Cells / Dental Pulp Type of study: Observational study Limits: Humans Language: English Journal: Med. oral patol. oral cir. bucal (Internet) Year: 2016 Document type: Article Institution/Affiliation country: University of Valencia/Spain
...