Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae
Mem. Inst. Oswaldo Cruz
; 104(4): 621-625, July 2009. tab
Article
in English
| LILACS
| ID: lil-523730
Responsible library:
BR1.1
ABSTRACT
Significant advances were made in the diagnosis of filariasis in the 1990s with the emergence of three new alternative tools ultrasound and tests to detect circulating antigen using two monoclonal antibodies, Og4C3 and AD12-ICT-card. This study aimed to identify which of these methods is the most sensitive for diagnosis of infection. A total of 256 individuals, all male and carrying microfilariae (1-15,679 MF/mL), diagnosed by nocturnal venous blood samples, were tested by all three techniques. The tests for circulating filarial antigen concurred 100 percent and correctly identified 246/256 (96.69 percent) of the positive individuals, while ultrasound detected only 186/256 (73.44 percent). Of the circulating antigen tests, ICT-card was the most convenient method for identification of Wuchereria bancrofti carriers. It was easy to perform, practical and quick.
Full text:
Available
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Helminthiasis
/
Neglected Diseases
/
Zoonoses
Database:
LILACS
Main subject:
Wuchereria bancrofti
/
Elephantiasis, Filarial
/
Antibodies, Helminth
/
Microfilariae
/
Antibodies, Monoclonal
/
Antigens, Helminth
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2009
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fiocruz/BR