Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review
J. venom. anim. toxins incl. trop. dis
; 16(4): 537-542, 2010.
Article
in English
| LILACS
| ID: lil-566169
Responsible library:
BR33.1
ABSTRACT
In recent years, the polymerase chain reaction (PCR) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time PCR (qPCR). Data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. The benefits of qPCR in relation to conventional PCR include speed, reproducibility and quantitative ability. In addition to operational advantages, qPCR is more sensitive and reproducible and may replace conventional PCR in diagnostic routines. Regarding visceral leishmaniasis, the possibility of deployment of real-time PCR in highly complex diagnoses (reference services) in endemic areas will facilitate a swift and safe return for patients. Moreover, the use of a technique that possesses elevated diagnostic sensitivity, and can monitor therapy and prevent relapses promotes broader prospects for the disease control.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Ligase Chain Reaction
/
Leishmaniasis, Visceral
Type of study:
Diagnostic study
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Journal subject:
Toxicology
Year:
2010
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Oswaldo Cruz Foundation/BR