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The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients* / Uso da Reação em Cadeia da Polimerase aninhada (PCR aninhada) para o diagnóstico precoce da infecção pelo Histoplasma capsulatum no soro e no sangue total de pacientes HIV positivos
Anais Brasileiros de Dermatologia; Dantas, Katia Cristina; Freitas, Roseli S.; Moreira, Adriana Pardini Vicentini; Silva, Marcos Vinicius da; Benard, Gil; Vasconcellos, Cidia; Criado, Paulo Ricardo.
Affiliation
  • Dantas, Katia Cristina; University of São Paulo. Pharmacist. Doctor of Science awarded by the Faculty of Medicine of the University of São Paulo (FMUSP) - researcher in the Faculty of Medicine. São Paulo. BR
  • Freitas, Roseli S.; University of São Paulo. Pharmacist. Doctor of Science awarded by the Faculty of Medicine of the University of São Paulo (FMUSP) - researcher in the Faculty of Medicine. São Paulo. BR
  • Moreira, Adriana Pardini Vicentini; University of São Paulo. Pharmacist. Doctor of Science awarded by the Faculty of Medicine of the University of São Paulo (FMUSP) - researcher in the Faculty of Medicine. São Paulo. BR
  • Silva, Marcos Vinicius da; University of São Paulo. Pharmacist. Doctor of Science awarded by the Faculty of Medicine of the University of São Paulo (FMUSP) - researcher in the Faculty of Medicine. São Paulo. BR
  • Benard, Gil; University of São Paulo. Pharmacist. Doctor of Science awarded by the Faculty of Medicine of the University of São Paulo (FMUSP) - researcher in the Faculty of Medicine. São Paulo. BR
  • Vasconcellos, Cidia; University of São Paulo. Pharmacist. Doctor of Science awarded by the Faculty of Medicine of the University of São Paulo (FMUSP) - researcher in the Faculty of Medicine. São Paulo. BR
  • Criado, Paulo Ricardo; University of São Paulo. Pharmacist. Doctor of Science awarded by the Faculty of Medicine of the University of São Paulo (FMUSP) - researcher in the Faculty of Medicine. São Paulo. BR
An. bras. dermatol ; 88(1): 141-143, fev. 2013.
Article in English | LILACS | ID: lil-667948
Responsible library: BR1.1
ABSTRACT
The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8). The PCR sequences HC18, HC100 and HC5.8 resulted in a specificity of 100%. The molecular assays may increase the specificity, sensitivity and speed in the diagnosis of Histoplasmosis.
RESUMO
O objetivo do estudo consistiu em detectar seqüências no ADNr e as suas regiões no Histoplasma capsulatum, que pudessem ser consideradas espécie-específicas e usadas como método molecular para o diagnóstico pela técnica da reação em cadeia da polimerase aninhada ("nested PCR") com seqüências específicas ("primers") para H. capsulatum regiões 18S ADNr (HC18), 100kDa (HC100) e a seqüência 5.8 S ADNr-ITS (HC5.8). A "nested PCR" com as seqüências HC18, HC100 e HC5.8 resultaram em 100% de especificidade. Os ensaios moleculares podem aumentar a especificidade, sensibilidade e rapidez na diagnose da Histoplasmose.
Subject(s)


Full text: Available Collection: International databases Database: LILACS Main subject: Polymerase Chain Reaction / HIV Seropositivity / Histoplasma / Histoplasmosis Type of study: Diagnostic study / Screening study Limits: Humans Language: English Journal: An. bras. dermatol Journal subject: Dermatology Year: 2013 Document type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR

Full text: Available Collection: International databases Database: LILACS Main subject: Polymerase Chain Reaction / HIV Seropositivity / Histoplasma / Histoplasmosis Type of study: Diagnostic study / Screening study Limits: Humans Language: English Journal: An. bras. dermatol Journal subject: Dermatology Year: 2013 Document type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR
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