Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems
Mem. Inst. Oswaldo Cruz
; 109(8): 978-983, 12/2014. graf
Article
in English
| LILACS
| ID: lil-732610
Responsible library:
BR1.1
ABSTRACT
The Global Program for the Elimination of Lymphatic Filariasis (GPELF) aims to eliminate this disease by the year 2020. However, the development of more specific and sensitive tests is important for the success of the GPELF. The present study aimed to standardise polymerase chain reaction (PCR)-based systems for the diagnosis of filariasis in serum and urine. Twenty paired biological urine and serum samples from individuals already known to be positive for Wuchereria bancrofti were collected during the day. Conventional PCR and semi-nested PCR assays were optimised. The detection limit of the technique for purified W. bancrofti DNA extracted from adult worms was 10 fg for the internal systems (WbF/Wb2) and 0.1 fg by using semi-nested PCR. The specificity of the primers was confirmed experimentally by amplification of 1 ng of purified genomic DNA from other species of parasites. Evaluation of the paired urine and serum samples by the semi-nested PCR technique indicated only two of the 20 tested individuals were positive, whereas the simple internal PCR system (WbF/Wb2), which has highly promising performance, revealed that all the patients were positive using both samples. This study successfully demonstrated the possibility of using the PCR technique on urine for the diagnosis of W. bancrofti infection.
Full text:
Available
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Helminthiasis
/
Neglected Diseases
/
Zoonoses
Database:
LILACS
Main subject:
Wuchereria bancrofti
/
Elephantiasis, Filarial
/
DNA, Helminth
/
Microfilariae
Type of study:
Diagnostic study
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2014
Document type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Centro de Pesquisas Aggeu Magalhães-Fiocruz/BR