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Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis
Sales, Mariana L.; Fonseca Júnior, Antônio Augusto; Orzil, Lívia; Alencar, Andrea Padilha; Silva, Marcio Roberto; Issa, Marina Azevedo; Soares Filho, Paulo Martins; Lage, Andrey Pereira; Heinemann, Marcos Bryan.
Affiliation
  • Sales, Mariana L.; Laboratório Nacional Agropecuário de Minas Gerais. Pedro Leopoldo. BR
  • Fonseca Júnior, Antônio Augusto; Laboratório Nacional Agropecuário de Minas Gerais. Pedro Leopoldo. BR
  • Orzil, Lívia; Laboratório Nacional Agropecuário de Minas Gerais. Pedro Leopoldo. BR
  • Alencar, Andrea Padilha; Laboratório Nacional Agropecuário de Minas Gerais. Pedro Leopoldo. BR
  • Silva, Marcio Roberto; Laboratório Nacional Agropecuário de Minas Gerais. Pedro Leopoldo. BR
  • Issa, Marina Azevedo; Laboratório Nacional Agropecuário de Minas Gerais. Pedro Leopoldo. BR
  • Soares Filho, Paulo Martins; Laboratório Nacional Agropecuário de Minas Gerais. Pedro Leopoldo. BR
  • Lage, Andrey Pereira; Laboratório Nacional Agropecuário de Minas Gerais. Pedro Leopoldo. BR
  • Heinemann, Marcos Bryan; Laboratório Nacional Agropecuário de Minas Gerais. Pedro Leopoldo. BR
Braz. j. microbiol ; 45(4): 1362-1369, Oct.-Dec. 2014. graf, tab
Article in English | LILACS | ID: lil-741288
Responsible library: BR1.1
ABSTRACT
Mycobacterium tuberculosis is the major cause of tuberculosis in humans. This bacillus gained prominence with the occurrence of HIV, presenting itself as an important opportunistic infection associated with acquired immunodeficiency syndrome (AIDS). The current study aimed to develop a real-time PCR using Eva Green technology for molecular identification of M. tuberculosis isolates. The primers were designed to Rv1510 gene. Ninety nine samples of M. tuberculosis and sixty samples of M. bovis were tested and no sample of the bovine bacillus was detected by the qPCR. Statistical tests showed no difference between the qPCR and biochemical tests used to identify the Mycobacterium tuberculosis. The correlation between tests was perfect with Kappa index of 1.0 (p < 0.001, CI = 0.84 - 1.0). The diagnostic sensitivity and specificity were 100% (CI = 95.94% - 100%) and 100% (CI = 93.98% - 100%). This qPCR was developed with the goal of diagnosing the bacillus M. tuberculosis in samples of bacterial suspension. TB reference laboratories (health and agriculture sectors), public health programs and epidemiological studies probably may benefit from such method.
Subject(s)


Full text: Available Collection: International databases Health context: Neglected Diseases Health problem: Neglected Diseases / Tuberculosis / Zoonoses Database: LILACS Main subject: Tuberculosis / Real-Time Polymerase Chain Reaction / Mycobacterium tuberculosis Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2014 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Laboratório Nacional Agropecuário de Minas Gerais/BR

Full text: Available Collection: International databases Health context: Neglected Diseases Health problem: Neglected Diseases / Tuberculosis / Zoonoses Database: LILACS Main subject: Tuberculosis / Real-Time Polymerase Chain Reaction / Mycobacterium tuberculosis Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2014 Document type: Article Affiliation country: Brazil Institution/Affiliation country: Laboratório Nacional Agropecuário de Minas Gerais/BR
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