WITHDRAWN: Selection of reference genes in five types of human tissuesfor normalization of gene expression studies in infectious diseases
Gene
; 12(16): 30816-30822, 2016.
Article
in English
| Sec. Est. Saúde SP, SESSP-IDPCPROD, Sec. Est. Saúde SP
| ID: biblio-1063066
Responsible library:
BR79.1
Localization: BR79.1
ABSTRACT
Gene expression analyses based on messenger RNA (mRNA) expression require accurate datanormalization. When using endogenous reference genes, these have to be carefully validated. Validated reference genes vary greatly depending on tissue, cell subsets and experimental context.The aim of this study was to identify reference genes that present more stable mRNA levels amongindividuals in peripheral blood mononuclear cells (PBMC); fresh skin biopsies; lung and brainautopsies as well as, skin biopsies formalin-fixed paraffin-embedded (FFPE). Therefore, 6endogenous reference genes were evaluated by quantitative real-time polymerase chain reaction 18SrRNA, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), TATA box-binding protein (TBP),beta-2-microbolin (B2M), ubiquitin C (UBC) and mitochondrially encoded ATP synthase 6 (MTATP6).Furthermore, validation of their stability and suitability as reference genes was determined bythe geNorm program. The results show that in PBMC and fresh skin biopsies, TBP and UBC wereidentified as the most stable, while in FFPE lung autopsies and skin biopsies, GAPDH and B2M, andin FFPE brain autopsies, GAPDH and UBC turned out to be the most stable...
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Collection:
National databases
/
Brazil
Database:
Sec. Est. Saúde SP
/
SESSP-IDPCPROD
Main subject:
Gene Expression
/
Real-Time Polymerase Chain Reaction
/
Genes
Language:
English
Journal:
Gene
Year:
2016
Document type:
Article
Institution/Affiliation country:
Centro de Parasitologia e Micologia, Instituto Adolfo Lutz/BR
/
Centro de Patologia do Instituto Adolfo Lutz/BR
/
Faculdade de Medicina de São José do Rio Preto/BR
/
Fundação Faculdade Regional de Medicina-Hospital de Base/BR
/
Instituto Dante Pazzanese de Cardiologia/BR
/
Laboratório Regional de Sorocaba, Instituto Adolfo Lutz/BR
/
Laboratório de Imunogenética, Departamento de Biologia Molecular, Faculdade de Medicina de São José do Rio Preto/BR