Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells
Rev. Soc. Bras. Med. Trop
; 51(2): 168-173, Mar.-Apr. 2018. graf
Artículo
en Inglés
| LILACS
| ID: biblio-897058
Biblioteca responsable:
BR1.1
ABSTRACT
Abstract INTRODUCTION:
Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year.METHODS:
In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR).RESULTS:
All three assays identified 29.1% (39/134) of the patients as dengue-positive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods.CONCLUSIONS:
The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Contexto en salud:
Enfermedades Desatendidas
Problema de salud:
Dengue
/
Enfermedades Desatendidas
Base de datos:
LILACS
Asunto principal:
Leucocitos Mononucleares
/
Dengue
/
Virus del Dengue
/
Anticuerpos Antivirales
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
Medicina Tropical
Año:
2018
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade de São Paulo/BR