Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism
Acta cir. bras
; 33(8): 652-663, Aug. 2018. graf
Artigo
em Inglês
| LILACS
| ID: biblio-949377
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To evaluate histopathological and ultrastructural changes and expression of proteins related to apoptosis CASPASE 3 and XIAP after experimental induction of temporary focal cerebral ischemia (90 minutes) due to obstruction of the middle cerebral artery in alcoholism model.Methods:
Forty adult Wistar rats were used, subdivided into 5 experimental groups control group (C); Sham group (S); Ischemic group (I); Alcoholic group (A); and Ischemic and Alcoholized group (I+A) animals submitted to the same treatment of group A and after four weeks were submitted to focal cerebral ischemia during 90 minutes, followed by reperfusion of 48 hours. Were processed for histopathological analysis and immunohistochemistry (for the protein expression of CASPASE -3 and XIAP).Results:
Greater histopathological changes were observed in the animals of groups I and I+A in the three areas analyzed. The neuronal loss was higher in the medial striatum region of the animals of groups I and I + A. The protein expression of CASPASE -3 was higher than that of XIAP in the groups I and I + A for both proteins.Conclusion:
The expression of XIAP was slightly higher where the histopathological changes and expression of CASPASE -3 was less evident.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Doença Cardiovascular
/
Doença Cerebrovascular
Base de dados:
LILACS
Assunto principal:
Ataque Isquêmico Transitório
/
Alcoolismo
/
Proteínas Inibidoras de Apoptose
/
Caspase 3
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Medical School/BR
/
USP/BR
/
Universidade Federal de Alagoas/BR
/
Universidade de São Paulo/BR