Identification and modeling of a novel chloramphenicol resistance protein detected by functional metagenomics in a wetland of Lerma, Mexico
Int. microbiol
; 16(2): 103-111, jun. 2013. ilus, tab
Artigo
em Inglês
| IBECS
| ID: ibc-126425
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
The exploration of novel antibiotic resistance determinants in a particular environment may be limited because of the presence of uncultured microorganisms. In this work, a culture independent approach based on functional metagenomics was applied to search for chloramphenicol resistance genes in agro-industrial wastewater in Lerma de Villada, Mexico. To this end, a metagenomic library was generated in Escherichia coli DH10B containing DNA isolated from environmental samples of the residual arsenic-enriched (10 mg/ml) effluent. One resistant clone was detected in this library and further analyzed. An open reading frame similar to a multidrug resistance protein from Aeromonas salmonicida and responsible for chloramphenicol resistance was identifi ed, sequenced, and found to encode a member of the major facilitator superfamily (MFS). Our results also showed that the expression of this gene restored streptomycin sensitivity in E. coli DH10B cells. To gain further insight into the phenotype of this MFS family member, we developed a model of the membrane protein multiporter that, in addition, may serve as a template for developing new antibiotics (AU)
RESUMEN
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Coleções:
Bases de dados nacionais
/
Espanha
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Zoonoses
Base de dados:
IBECS
Assunto principal:
Resistência ao Cloranfenicol
/
Metagenômica
Tipo de estudo:
Estudo diagnóstico
País/Região como assunto:
México
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2013
Tipo de documento:
Artigo
Instituição/País de afiliação:
Center of Astrobiology (CSIC-INTA)/Spain
/
Metropolitan Autononous University (Lerma Unit)/México