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Is microarray analysis really useful and sufficient to diagnose nut allergy in the mediterranean area?
Goikoetxea, MJ; D’Amelio, CM; Martínez-Aranguren, R; Gamboa, P; Garcia, BE; Gómez, F; Fernández, J; Bartra, J; Parra, A; Alvarado, MI; Alonso, MI; González, E; Terrados, S; Moya, C; Blanca, N; Feo-Brito, F; Villalba, M; Díaz-Perales, A; Sanz, ML.
Afiliação
  • Goikoetxea, MJ; Clinica Universidad de Navarra. Department of Allergy and Clinical Immunology. Instituto de Investigación Sanitaria de Navarra. Pamplona. Spain
  • D’Amelio, CM; Clinica Universidad de Navarra. Department of Allergy and Clinical Immunology. Instituto de Investigación Sanitaria de Navarra. Pamplona. Spain
  • Martínez-Aranguren, R; Clinica Universidad de Navarra. Department of Allergy and Clinical Immunology. Instituto de Investigación Sanitaria de Navarra. Pamplona. Spain
  • Gamboa, P; Hospital de Basurto. Allergy Department. Bilbao. Spain
  • Garcia, BE; Complejo Hospitalario de Navarra. Allergy Department. Instituto de Investigación Sanitaria de Navarra. Pamplona. Spain
  • Gómez, F; Hospital Regional Universitario de Málaga. Allergy Department. Instituto de Investigación Biomédica de Málaga (IBIMA). Málaga. Spain
  • Fernández, J; Hospital General Universitario de Alicante. Allergy Department. Alicante. Spain
  • Bartra, J; Universitat de Barcelona. Hospital Clinic. Allergy, Respiratory, and Respiratory Allergy Department. Barcelona. Spain
  • Parra, A; Hospital de A Coruña. Allergy Department. A Coruña. Spain
  • Alvarado, MI; Hospital Virgen del Puerto. Allergy Department. Plasencia. Spain
  • Alonso, MI; Hospital de Alcorcón. Allergy Department. Madrid. Spain
  • González, E; Hospital de Fuenlabrada. Allergy Department. Madrid. Spain
  • Terrados, S; Hospital Ramón y Cajal. Allergy Department. Madrid. Spain
  • Moya, C; Complejo Hopitalario Torrecárdenas. Allergy Department. Almeria. Spain
  • Blanca, N; Hospital Infanta Leonor. Allergy Department. Madrid. Spain
  • Feo-Brito, F; Hospital General Universitario de Ciudad Real. Allergy Department. Ciudad Real. Spain
  • Villalba, M; Universidad Complutense. Facultad de Químicas. Molecular Biology Department. Madrid. Spain
  • Díaz-Perales, A; Centro de Biotecnología y Genómica de Plantas. Biotechnology Department. Pozuelo de Alarcón. Spain
  • Sanz, ML; Clinica Universidad de Navarra. Department of Allergy and Clinical Immunology. Instituto de Investigación Sanitaria de Navarra. Pamplona. Spain
J. investig. allergol. clin. immunol ; 26(1): 31-39, 2016. tab, ilus, graf
Artigo em Inglês | IBECS | ID: ibc-150187
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT

Background:

Component-based diagnosis on multiplex platforms is widely used in food allergy but its clinical performance has not been evaluated in nut allergy.

Objective:

To assess the diagnostic performance of a commercial protein microarray in the determination of specific IgE (sIgE) in peanut, hazelnut, and walnut allergy.

Methods:

sIgE was measured in 36 peanut-allergic, 36 hazelnut-allergic, and 44 walnut-allergic patients by ISAC 112, and subsequently, sIgE against available components was determined by ImmunoCAP in patients with negative ISAC results. ImmunoCAP was also used to measure sIgE to Ara h 9, Cor a 8, and Jug r 3 in a subgroup of lipid transfer protein (LTP)-sensitized nut-allergic patients (positive skin prick test to LTP-enriched extract). sIgE levels by ImmunoCAP were compared with ISAC ranges.

Results:

Most peanut-, hazelnut-, and walnut-allergic patients were sensitized to the corresponding nut LTP (Ara h 9, 66.7%; Cor a 8, 80.5%; Jug r 3, 84% respectively). However, ISAC did not detect sIgE in 33.3% of peanut-allergic patients, 13.9% of hazelnut-allergic patients, or 13.6% of walnut-allergic patients. sIgE determination by ImmunoCAP detected sensitization to Ara h 9, Cor a 8, and Jug r 3 in, respectively, 61.5% of peanut-allergic patients, 60% of hazelnut-allergic patients, and 88.3% of walnut-allergic patients with negative ISAC results. In the subgroup of peach LTP-sensitized patients, Ara h 9 sIgE was detected in more cases by ImmunoCAP than by ISAC (94.4% vs 72.2%, P<.05). Similar rates of Cor a 8 and Jug r 3 sensitization were detected by both techniques.

Conclusions:

The diagnostic performance of ISAC was adequate for hazelnut and walnut allergy but not for peanut allergy. sIgE sensitivity against Ara h 9 in ISAC needs to be improved (AU)
RESUMEN

Introducción:

La utilidad clínica del diagnóstico por componentes no ha sido evaluada en el estudio de la alergia a frutos secos (FS).

Objetivo:

Evaluar la capacidad diagnóstica de una micromatriz comercial de proteínas alergénicas en la alergia a cacahuete, avellana y nuez.

Métodos:

Se determinó la sIgE en pacientes alérgicos a FS mediante la micromatriz ISAC 112, e ImmunoCAP en los pacientes con sIgE negativa frente a los componentes de ISAC. Además, se realizó ImmunoCAP frente a Ara h 9, Cor a 8 y Jug r 3 en un subgrupo de pacientes sensibilizados a LTP. La sIgE detectada por ImmunoCAP fue comparada con los rangos de ISAC.

Resultados:

La mayoría de los alérgicos a cacahuete (66,7%), avellana (80,5%) y nuez (84%) estaba sensibilizados a su LTP. Sin embargo, no se detectó sIgE frente a los componentes de ISAC en el 33,3% de alérgicos a cacahuete, 13,9% de alérgicos a avellana y 13,6% de los alérgicos a nuez. El ImmunoCAP permitió detectar sIgE a Ara h 9 en 61,5%, Cor a 8 en 60% y Jug r 3 en 83,3% de los ISAC negativo. En el subgrupo LTP, ImmunoCAP (94,4%) fue superior a ISAC (72,2%) en la detección de sIgE a Ara h 9 (p<0,05). La sIgE frente a Cor a 8 y Jug r 3 fue detectada de forma similar por ambas técnicas.

Conclusiones:

La micromatriz ISAC es adecuada para el diagnóstico de alergia a avellana y nuez. La sensibilidad del componente Ara h 9 de ISAC debe ser mejorada (AU)
Assuntos

Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Arachis / Testes Imunológicos / Técnicas Imunológicas / Hipersensibilidade a Noz / Hipersensibilidade a Amendoim / Corylus / Hipersensibilidade Tipo de estudo: Estudo diagnóstico / Estudo de avaliação Limite: Adolescente / Feminino / Humanos / Masculino Idioma: Inglês Revista: J. investig. allergol. clin. immunol Ano de publicação: 2016 Tipo de documento: Artigo Instituição/País de afiliação: Centro de Biotecnología y Genómica de Plantas/Spain / Clinica Universidad de Navarra/Spain / Complejo Hopitalario Torrecárdenas/Spain / Complejo Hospitalario de Navarra/Spain / Hospital General Universitario de Alicante/Spain / Hospital General Universitario de Ciudad Real/Spain / Hospital Infanta Leonor/Spain / Hospital Ramón y Cajal/Spain / Hospital Regional Universitario de Málaga/Spain / Hospital Virgen del Puerto/Spain

Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Arachis / Testes Imunológicos / Técnicas Imunológicas / Hipersensibilidade a Noz / Hipersensibilidade a Amendoim / Corylus / Hipersensibilidade Tipo de estudo: Estudo diagnóstico / Estudo de avaliação Limite: Adolescente / Feminino / Humanos / Masculino Idioma: Inglês Revista: J. investig. allergol. clin. immunol Ano de publicação: 2016 Tipo de documento: Artigo Instituição/País de afiliação: Centro de Biotecnología y Genómica de Plantas/Spain / Clinica Universidad de Navarra/Spain / Complejo Hopitalario Torrecárdenas/Spain / Complejo Hospitalario de Navarra/Spain / Hospital General Universitario de Alicante/Spain / Hospital General Universitario de Ciudad Real/Spain / Hospital Infanta Leonor/Spain / Hospital Ramón y Cajal/Spain / Hospital Regional Universitario de Málaga/Spain / Hospital Virgen del Puerto/Spain
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