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ApoA-I induces S1P release from endothelial cells through ABCA1 and SR-BI in a positive feedback manner
Liu, Xing; Ren, Kun; Suo, Rong; Xiong, Sheng-Lin; Zhang, Qing-Hai; Mo, Zhong-Cheng; Tang, Zhen-Li; Jiang, Yue; Peng, Xiao-Shan; Yi, guang-Hui.
Afiliação
  • Liu, Xing; University of South China. Institute of Cardiovascular Disease. Key Lab for Arteriosclerology of Hunan Province. Hengyang City. China
  • Ren, Kun; University of South China. Institute of Cardiovascular Disease. Key Lab for Arteriosclerology of Hunan Province. Hengyang City. China
  • Suo, Rong; University of South China. Institute of Cardiovascular Disease. Key Lab for Arteriosclerology of Hunan Province. Hengyang City. China
  • Xiong, Sheng-Lin; You Country People's Hospital. Zhuzhou. China
  • Zhang, Qing-Hai; The First Affiliated Hospital. Clinical Research Institution. Hengyang. China
  • Mo, Zhong-Cheng; University of South China. Institute of Cardiovascular Research. Key Laboratory for Atherosclerology of Hunan Province. Hengyang. China
  • Tang, Zhen-Li; University of South China. Institute of Cardiovascular Disease. Key Lab for Arteriosclerology of Hunan Province. Hengyang City. China
  • Jiang, Yue; University of South China. Institute of Cardiovascular Disease. Key Lab for Arteriosclerology of Hunan Province. Hengyang City. China
  • Peng, Xiao-Shan; University of South China. Institute of Cardiovascular Disease. Key Lab for Arteriosclerology of Hunan Province. Hengyang City. China
  • Yi, guang-Hui; University of South China. Institute of Cardiovascular Disease. Key Lab for Arteriosclerology of Hunan Province. Hengyang City. China
J. physiol. biochem ; 72(4): 657-667, dic. 2016. graf, ilus
Artigo em Inglês | IBECS | ID: ibc-168373
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT
Sphingosine-1-phosphate (S1P), which has emerged as a pivotal signaling mediator that participates in the regulation of multiple cellular processes, is derived from various cells, including vascular endothelial cells. S1P accumulates in lipoproteins, especially HDL, and the majority of free plasma S1P is bound to HDL. We hypothesized that HDL-associated S1P is released through mechanisms associated with the HDL maturation process. ApoA-I, a major HDL apolipoprotein, is a critical factor for nascent HDL formation and lipid trafficking via ABCA1. Moreover, apoA-I is capable of promoting bidirectional lipid movement through SR-BI. In the present study, we confirmed that apoA-I can facilitate the production and release of S1P by HUVECs. Furthermore, we demonstrated that ERK1/2 and SphK activation induced by apoA-I is involved in the release of S1P from HUVECs. Inhibitor and siRNA experiments showed that ABCA1 and SR-BI are required for S1P release and ERK1/2 phosphorylation induced by apoA-I. However, the effects triggered by apoA-I were not suppressed by inhibiting ABCA1/JAK2 or the SR-BI/Src pathway. S1P released due to apoA-I activation can stimulate the (ERK1/2)/SphK1 pathway through S1PR (S1P receptor) 1/3. These results indicated that apoA-I not only promotes S1P release through ABCA1 and SR-BI but also indirectly activates the (ERK1/2)/SphK1 pathway by releasing S1P to trigger their receptors. In conclusion, we suggest that release of S1P induced by apoA-I from endothelial cells through ABCA1 and SR-BI is a self-positive-feedback process apoA-I-(ABCA1 and SR-BI)-(S1P release)-S1PR-ERK1/2-SphK1-(S1P production)-(more S1P release induced by apoA-I) (AU)
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Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Esfingosina / Lisofosfolipídeos / Apolipoproteína A-I / Proteína Quinase 1 Ativada por Mitógeno / Proteína Quinase 3 Ativada por Mitógeno / Proteínas Adaptadoras de Transdução de Sinal / Receptores Depuradores Classe B / Transportador 1 de Cassete de Ligação de ATP Limite: Humanos Idioma: Inglês Revista: J. physiol. biochem Ano de publicação: 2016 Tipo de documento: Artigo Instituição/País de afiliação: The First Affiliated Hospital/China / University of South China/China / You Country People's Hospital/China
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Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Esfingosina / Lisofosfolipídeos / Apolipoproteína A-I / Proteína Quinase 1 Ativada por Mitógeno / Proteína Quinase 3 Ativada por Mitógeno / Proteínas Adaptadoras de Transdução de Sinal / Receptores Depuradores Classe B / Transportador 1 de Cassete de Ligação de ATP Limite: Humanos Idioma: Inglês Revista: J. physiol. biochem Ano de publicação: 2016 Tipo de documento: Artigo Instituição/País de afiliação: The First Affiliated Hospital/China / University of South China/China / You Country People's Hospital/China
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