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Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis
International Braz J Urol; Ornellas, Paulo; Ornellas, Antonio Augusto; Chinello, Clizia; Gianazza, Erica; Mainini, Veronica; Cazzaniga, Marta; Pereira, Denise Abreu; Sandim, Vanessa; Cypriano, Ana Sheila; Koifman, Leandro; Silva, Paulo Cesar Barbosa da; Alves, Gilda; Magni, Fulvio.
Afiliação
  • Ornellas, Paulo; Hematology Service (PO, DAP, VS, ASC, GA). Laboratory of Applied Genetics. Rio de Janeiro. BR
  • Ornellas, Antonio Augusto; Hematology Service (PO, DAP, VS, ASC, GA). Laboratory of Applied Genetics. Rio de Janeiro. BR
  • Chinello, Clizia; Hematology Service (PO, DAP, VS, ASC, GA). Laboratory of Applied Genetics. Rio de Janeiro. BR
  • Gianazza, Erica; Hematology Service (PO, DAP, VS, ASC, GA). Laboratory of Applied Genetics. Rio de Janeiro. BR
  • Mainini, Veronica; Hematology Service (PO, DAP, VS, ASC, GA). Laboratory of Applied Genetics. Rio de Janeiro. BR
  • Cazzaniga, Marta; Hematology Service (PO, DAP, VS, ASC, GA). Laboratory of Applied Genetics. Rio de Janeiro. BR
  • Pereira, Denise Abreu; Hematology Service (PO, DAP, VS, ASC, GA). Laboratory of Applied Genetics. Rio de Janeiro. BR
  • Sandim, Vanessa; Hematology Service (PO, DAP, VS, ASC, GA). Laboratory of Applied Genetics. Rio de Janeiro. BR
  • Cypriano, Ana Sheila; Hematology Service (PO, DAP, VS, ASC, GA). Laboratory of Applied Genetics. Rio de Janeiro. BR
  • Koifman, Leandro; Hematology Service (PO, DAP, VS, ASC, GA). Laboratory of Applied Genetics. Rio de Janeiro. BR
  • Silva, Paulo Cesar Barbosa da; Hematology Service (PO, DAP, VS, ASC, GA). Laboratory of Applied Genetics. Rio de Janeiro. BR
  • Alves, Gilda; Hematology Service (PO, DAP, VS, ASC, GA). Laboratory of Applied Genetics. Rio de Janeiro. BR
  • Magni, Fulvio; Hematology Service (PO, DAP, VS, ASC, GA). Laboratory of Applied Genetics. Rio de Janeiro. BR
Int. braz. j. urol ; 38(6): 739-749, Nov-Dec/2012. tab, graf
Artigo em Inglês | LILACS | ID: lil-666021
Biblioteca responsável: BR1.1
ABSTRACT
Purpose

id="para1">To investigate the use of ClinProt technique to identify cancer markers in plasma of patients suffering from squamous cell carcinoma of the penis (SCCP). Materials and <a class="decs" id="q000379">Methods</a>

id="para2">Plasma of 36 healthy subjects and 25 patients with penile carcinoma who underwent surgical treatment between June 2010 and June 2011 was collected and analyzed by the ClinProt/MALDI/ToF technique. Then the peptides were identified from the C8 MB eluted fraction of patients' and control subjects' plasma by LIFT MS/MS. Results

id="para3">A cluster of 2 peptides (A=m/z 1897.22 ± 9 Da and B=m/z 2021.99 ± 9 Da) was able to discriminate patients from control subjects. Cross validation analysis using the whole casuistic showed 62.5% and 86.76% sensitivity and specificity, respectively. The cluster also showed very high sensitivity (100%) and specificity (97%) for SCCP patients that died due to the disease. Furthermore, patients with lymph node involvement presented sensitivity and specificity of 80% and 97%, respectively. These two peptides were identified by the proteomic approach based on a MALDI-TOF/TOF as fragments of C3 (m/z 1896.17) and C4a/b (m/z 2021.26) complement proteins. Conclusions

id="para4">The results showed that as the disease progresses, the fragments C3 and C4 A/B are less expressed in comparison with healthy subjects. These results may be useful as prognostic tools. .

Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Neoplasias Penianas / Carcinoma de Células Escamosas Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Limite: Adulto / Idoso / Idoso, 80 anos ou mais / Humanos / Masculino Idioma: Inglês Revista: Int. braz. j. urol Assunto da revista: Urologia Ano de publicação: 2012 Tipo de documento: Artigo / Documento de projeto País de afiliação: Brasil Instituição/País de afiliação: Hematology Service (PO, DAP, VS, ASC, GA)/BR

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Neoplasias Penianas / Carcinoma de Células Escamosas Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Limite: Adulto / Idoso / Idoso, 80 anos ou mais / Humanos / Masculino Idioma: Inglês Revista: Int. braz. j. urol Assunto da revista: Urologia Ano de publicação: 2012 Tipo de documento: Artigo / Documento de projeto País de afiliação: Brasil Instituição/País de afiliação: Hematology Service (PO, DAP, VS, ASC, GA)/BR
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