Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds
Acta cir. bras
; 29(8): 485-492, 08/2014. graf
Artigo
em Inglês
| LILACS
| ID: lil-719186
Biblioteca responsável:
BR1.1
ABSTRACT
PURPOSE:
To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal.METHODS:
This study was performed with New Zealand adult rabbits weighing 3.0 - 4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase).RESULTS:
Generally, the cells surrounding soft tissues were successfully removed, but none protocol removed cells from the tracheal cartilage. However, longer protocols were more effective. The cost-benefits relation of the enzymatic processes was not favorable. It was possible to find out that the cartilaginous tissue submitted to the irradiation with LED 630nm and 475 nm showed an increased number of gaps without cells, but several cells were observed to be still present.CONCLUSION:
The light-emitting diode is a promising tool for decellularization of soft tissues. .
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Traqueia
/
Engenharia Tecidual
/
Tecidos Suporte
/
Luz
Tipo de estudo:
Estudo de avaliação
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2014
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Paulista State University/BR