Validation of the rapid test carestart(tm) G6PD among malaria vivax-infected subjects in the Brazilian Amazon
Rev. Soc. Bras. Med. Trop
; 49(4): 446-455, July-Aug. 2016. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-792792
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract INTRODUCTION:
In the Brazilian Amazon, malaria infections are primarily caused by Plasmodium vivax. The only drug that kills the hypnozoite form of P. vivax is primaquine, thereby preventing relapse. However, treating glucose-6-phosphate dehydrogenase (G6PD)-deficient individuals with primaquine can lead to severe hemolysis. G6PD deficiency (G6PDd) affects approximately 400 million people worldwide, most of whom live in malaria-endemic areas. Therefore, clinicians need tools that can easily and reliably identify individuals with G6PDd. This study estimated the accuracy of the Carestart(tm) G6PD rapid test (Access Bio) in the diagnosis of G6PDd in male participants with and without P. vivax acute malaria.METHODS:
Male participants were recruited in Manaus. Malaria diagnosis was determined by thick blood smear. G6PD quantitative analysis was performed spectro photometrically at a wave length of 340nm. The Carestart(tm) G6PD test was performed using venous blood. Genotyping was performed for individuals whose samples had an enzyme activity less than 70% of the normal value.RESULTS:
Six hundred and seventy-four male participants were included in this study, of whom 320 had a diagnosis of P. vivax malaria. In individuals with enzyme activity lower than 30% (n=13), the sensitivity, specificity, positive predictive value, and negative predictive value of the Carestart(tm) G6PD test were as follows 61.5% (95%CI 35.5%-82.3%), 98.3% (95%CI 97.0%-99.1%), 42.1% (95%CI 23.1%-63.7%), and 99.2% (95%CI 98.2%-82.3%), 98.3% (95%CI 97.0%-99.1%), 42.1% (95%CI 23.1%-63.7%), and 99.2% (95%CI 98.2%-99.7%), respectively. Increases in sensitivity were observed when increasing the cut-off value.CONCLUSIONS:
Despite low sensitivity, Carestart(tm) G6PD remains a good alternative for rapid diagnosis of G6PDd in malaria-endemic regions.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
/
Doenças Negligenciadas
Problema de saúde:
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
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Malária
/
Doenças Negligenciadas
Base de dados:
LILACS
Assunto principal:
Malária Vivax
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Sistemas Automatizados de Assistência Junto ao Leito
/
Glucosefosfato Desidrogenase
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Adolescente
/
Adulto
/
Idoso
/
Criança
/
Humanos
/
Masculino
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
2016
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Fundação de Medicina Tropical Dr. Heitor Vieira Dourado/BR