Morphological and immunohistochemical analysis of apoptosis in the cerebellum of rats subjected to focal cerebral ischemia with or without alcoholism model
Acta cir. bras
; 31(9): 629-637, Sept. 2016. graf
Artigo
em Inglês
| LILACS
| ID: lil-795996
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT PURPOSE:
To evaluated histopathological changes, morphometric and expression of proteins CASPASE-3, BCL-2 and XIAP related to apoptosis in the cerebellum after induction of temporary focal cerebral ischemia followed by reperfusion, with or without a model of chronic alcoholism.METHODS:
Fifty Wistar rats were used and divided into control group (C), sham group (S), ischemic group (I), alcoholic group (A), and ischemic and alcoholic group (IA). The cerebellum samples collected were stained for histopathological and morphometric analysis and immunohistochemistry study.RESULTS:
Histopathological changes were observed a greater degree in animals in groups A and IA. The morphometric study showed no difference in the amount of cells in the granular layer of the cerebellum between the groups. The expression of CASPASE-3 was higher than BCL-2 and XIAP in the groups A and IA.CONCLUSION:
We observed correlation between histopathological changes and the occurrence of apoptosis in cerebellar cortex.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Neoplasias do Cérebro e Sistema Nervoso
/
Doença Cardiovascular
/
Doença Cerebrovascular
Base de dados:
LILACS
Assunto principal:
Cerebelo
/
Isquemia Encefálica
/
Apoptose
/
Etanol
/
Alcoolismo
/
Proteínas Reguladoras de Apoptose
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2016
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade de São Paulo/BR