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Purification of Total RNA from DSS-treated Murine Tissue via Lithium Chloride Precipitation.

Bio Protoc; 8(9)2018 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-29951571
We have developed a protocol to purify RNA from DSS (Dextran Sulfate Sodium)-treated mouse tissues. This method, which prevents downstream inhibition of q-RT-PCR observed in DSS-treated tissues, relies on successive precipitations with lithium chloride.