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Grafting as a Tool for Study of Phototropism in Phycomyces.

Methods Mol Biol; 1924: 199-206, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30694477
Physiological responses, such as phototropism and carotenogenesis, are usually exhibited through a specific cascade composed of several gene functions. The number of such gene functions can be determined by means of complementation analysis. For this purpose, a procedure is needed to produce heterokaryons easily and with a high success rate. Here, we present a method of grafting sporangiophores from different mutants to obtain heterokaryotic regenerates at the graft union, based on the large size of the sporangiophore and its remarkable regeneration capability.