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Small Molecule Inhibitors Specifically Targeting the Type III Secretion System of on Rice.

Int J Mol Sci; 20(4)2019 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-30813400
The initiative strategy for the development of novel anti-microbial agents usually uses the virulence factors of bacteria as a target, without affecting their growth and survival. The type III secretion system (T3SS), one of the essential virulence factors in most Gram-negative pathogenic bacteria because of its highly conserved construct, has been regarded as an effective target that developed new anti-microbial drugs. pv. ( ) causes leaf blight diseases and is one of the most important pathogens on rice. To find potential anti-virulence agents against this pathogen, a number of natural compounds were screened for their effects on the T3SS of . Three of 34 compounds significantly inhibited the promoter activity of the harpin gene, , and were further checked for their impact on bacterial growth and on the hypersensitive response (HR) caused by on non-host tobacco plants. The results indicated that treatment of with CZ-1, CZ-4 and CZ-9 resulted in an obviously attenuated HR without affecting bacterial growth and survival. Moreover, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that the expression of the T3SS was suppressed by treatment with the three inhibitors. The mRNA levels of representative genes in the hypersensitive response and pathogenicity ( ) cluster, as well as the regulatory genes and , were reduced. Finally, the in vivo test demonstrated that the compounds could reduce the disease symptoms of on the rice cultivar ( ) IR24.