Your browser doesn't support javascript.

Portal Regional da BVS

Informação e Conhecimento para a Saúde

Home > Pesquisa > ()
Imprimir Exportar

Formato de exportação:


Adicionar mais destinatários
| |

A novel fluoro-chromogenic Cu probe for living-cell imaging based on rhodamine 6G-pyridine conjugation.

Anal Bioanal Chem; 2019 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-30888466
A novel fluoro-chromogenic rhodamine spirolactam probe (RP) has been prepared through the condensation of rhodamine hydrazine and 2-acetylpyridine, which displayed the detection of Cu with high selectivity over a large number of other common metal ions. It shows a "turn-on" response to paramagnetic Cu with an about 12-fold enhancement, and a color change from colorless to red that is observable by the naked eye. These changes are ascribed to the ring-opening of the spirolactam in RP, and subsequent host-guest coordination. The 2:1 binding stoichiometry of RP to Cu was confirmed by Job's and B-H plots. The resulting fluorescence enhancement can be used to detect Cu at concentrations from 2.0 to 20.0 µM with a limit of detection of 0.21 µM, which was lower than the maximum allowable Cu level set by the WHO. Finally, RP has been utilized to monitor Cu in living cells and natural water. Graphical abstract.