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Liquid and Frozen Storage of Agouti (Dasyprocta leporina) Semen Extended with UHT Milk, Unpasteurized Coconut Water, and Pasteurized Coconut Water
Mollineau, W. M.; Adogwa, A. O. ; Garcia, G. W. .
Afiliação
  • Mollineau, W. M.; The University of the West Indies. Faculty of Science and Agriculture. Department of Food Production. St. Augustine. Trinidad and Tobago
  • Adogwa, A. O. ; The University of the West Indies. Faculty of Medical Sciences. School of Veterinary Medicine. St. Augustine. Trinidad and Tobago
  • Garcia, G. W. ; The University of the West Indies. Faculty of Science and Agriculture. Department of Food Production. St. Augustine. Trinidad and Tobago
Veterinary medicine international ; 2011: [1-5], Sep. 2010. tab, graf
Artigo em Inglês | MedCarib | ID: med-17637
Biblioteca responsável: TT5
ABSTRACT
This study evaluated the effects of semen extension and storage on forward progressive motility % (FPM%) in agouti semen. Three extenders were used; sterilized whole cow's milk (UHT Milk), unpasteurized (CW) and pasteurized coconut water (PCW), and diluted to 50, 100, 150, and 200 ž 10(6) spermatozoa/ml. Experiment 1 200 ejaculates were extended for liquid storage at 5(∘)C and evaluated every day for 5 days to determine FPM% and its rate of deterioration. Experiment 2 150 ejaculates were extended for storage as frozen pellets in liquid nitrogen at -195(∘)C, thawed at 30(∘) to 70(∘)C for 20 to 50 seconds after 5 days and evaluated for FPM% and its rate of deterioration. Samples treated with UHT milk and storage at concentrations of 100 ž 10(6) spermatozoa/ml produced the highest means for FPM% and the slowest rates of deterioration during Experiment 1. During Experiment 2 samples thawed at 30(∘)C for 20 seconds exhibited the highest means for FPM% (12.18 ñ 1.33%), 85% rate of deterioration. However, samples were incompletely thawed. This was attributed to the diameter of the frozen pellets which was 1 cm. It was concluded that the liquid storage method was better for short term storage.
Assuntos
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MedCarib Assunto principal: Preservação do Sêmen / Motilidade dos Espermatozoides / Espermatozoides / Trinidad e Tobago / Criopreservação / Substitutos do Leite Humano Limite: Animais País/Região como assunto: Caribe Inglês / Trinidad e Tobago Idioma: Inglês Revista: Veterinary medicine international Ano de publicação: 2010 Tipo de documento: Artigo Instituição/País de afiliação: The University of the West Indies/Trinidad and Tobago
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MedCarib Assunto principal: Preservação do Sêmen / Motilidade dos Espermatozoides / Espermatozoides / Trinidad e Tobago / Criopreservação / Substitutos do Leite Humano Limite: Animais País/Região como assunto: Caribe Inglês / Trinidad e Tobago Idioma: Inglês Revista: Veterinary medicine international Ano de publicação: 2010 Tipo de documento: Artigo Instituição/País de afiliação: The University of the West Indies/Trinidad and Tobago
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