Liquid and Frozen Storage of Agouti (Dasyprocta leporina) Semen Extended with UHT Milk, Unpasteurized Coconut Water, and Pasteurized Coconut Water
Veterinary medicine international
; 2011: [1-5], Sep. 2010. tab, graf
Artigo
em Inglês
| MedCarib
| ID: med-17637
Biblioteca responsável:
TT5
ABSTRACT
This study evaluated the effects of semen extension and storage on forward progressive motility % (FPM%) in agouti semen. Three extenders were used; sterilized whole cow's milk (UHT Milk), unpasteurized (CW) and pasteurized coconut water (PCW), and diluted to 50, 100, 150, and 200 10(6) spermatozoa/ml. Experiment 1 200 ejaculates were extended for liquid storage at 5(∘)C and evaluated every day for 5 days to determine FPM% and its rate of deterioration. Experiment 2 150 ejaculates were extended for storage as frozen pellets in liquid nitrogen at -195(∘)C, thawed at 30(∘) to 70(∘)C for 20 to 50 seconds after 5 days and evaluated for FPM% and its rate of deterioration. Samples treated with UHT milk and storage at concentrations of 100 10(6) spermatozoa/ml produced the highest means for FPM% and the slowest rates of deterioration during Experiment 1. During Experiment 2 samples thawed at 30(∘)C for 20 seconds exhibited the highest means for FPM% (12.18 ñ 1.33%), 85% rate of deterioration. However, samples were incompletely thawed. This was attributed to the diameter of the frozen pellets which was 1 cm. It was concluded that the liquid storage method was better for short term storage.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
MedCarib
Assunto principal:
Preservação do Sêmen
/
Motilidade dos Espermatozoides
/
Espermatozoides
/
Trinidad e Tobago
/
Criopreservação
/
Substitutos do Leite Humano
Limite:
Animais
País/Região como assunto:
Caribe Inglês
/
Trinidad e Tobago
Idioma:
Inglês
Revista:
Veterinary medicine international
Ano de publicação:
2010
Tipo de documento:
Artigo
Instituição/País de afiliação:
The University of the West Indies/Trinidad and Tobago