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Low-Level Laser Irradiation (InGaAlP-660 nm) Increases Fibroblast Cell Proliferation and Reduces Cell Death in a Dose-Dependent Manner
Frigo, Lucio; Favero, Giovani M; Lima, Haroldo J. Campos; Maria, Durvanei Augusto; Bjordal, Jan M; Joensen, Jon; Iversen, Vegard V; Marcos, Rodrigo Labat; Parizzoto, Nivaldo Antonio; Lopes Martins, Rodrigo Alvaro Brandão.
Afiliação
  • Frigo, Lucio; s.af
  • Favero, Giovani M; s.af
  • Lima, Haroldo J. Campos; s.af
  • Maria, Durvanei Augusto; Instituto Butantan. São Paulo. BR
  • Bjordal, Jan M; s.af
  • Joensen, Jon; s.af
  • Iversen, Vegard V; s.af
  • Marcos, Rodrigo Labat; s.af
  • Parizzoto, Nivaldo Antonio; s.af
  • Lopes Martins, Rodrigo Alvaro Brandão; s.af
Photomedicine and laser surgery ; 28(1): 151-156, 2010.
Artigo em Inglês | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO | ID: biblio-1065493
Biblioteca responsável: BR78.1
Localização: BR78.1
ABSTRACT
Background and

Objective:

Impaired cell metabolism and increased cell death in fibroblast cells are physiologicalfeatures of chronic tendinopathy. Although several studies have shown that low-level laser therapy (LLLT) atcertain parameters has a biostimulatory effect on fibroblast cells, it remains uncertain if LLLT effects depend on thephysiological state. Study Design/Material and

Methods:

High-metabolic immortal cell culture and primaryhuman keloid fibroblast cell culture were used in this study. Trypan blue exclusion and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test were used to determine cell viability and proliferation. Propidiumiodide stain was used for cell-cycle analysis by flow cytometry. Laser irradiation was performed daily onthree consecutive days with a GaAlAs 660-nm laser (mean output 50mW, spot size 2mm2, power density»2.5W=cm2) and a typical LLLT dose and a high LLLT dose (irradiation times 60 or 420 s; fluences150 or1050 J=cm2; energy delivered 3 or 21 J).

Results:

Primary fibroblast cell culture from human keloids irradiated with3 J showed significant proliferation by the trypan blue exclusion test ( p<0.05), whereas the 3T3 cell cultureshowed no difference using this method. Propidium iodide staining flow cytometry data showed a significantdecrease in the percentage of cells being in proliferative phases of the cell cycle (S=g2=M) when irradiated with 21 Jin both cell types (hypodiploid cells increased).

Conclusions:

Our data support the hypothesis that the physiologicalstate of the cells affects the LLLT results, and that high-metabolic rate and short- cell-cycle 3T3 cells are notresponsive to LLLT. In conclusion, LLLT with a dose of 3 J reduced cell death significantly, but did not stimulatecell cycle. A LLLT dose of 21 J had negative effects on the cells, as it increased cell death and inhibited cellproliferation.
Assuntos
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Coleções: Bases de dados nacionais / Brasil Base de dados: Sec. Est. Saúde SP / SESSP-IBACERVO / SESSP-IBPROD Assunto principal: Células / Fibroblastos Idioma: Inglês Revista: Photomedicine and laser surgery Ano de publicação: 2010 Tipo de documento: Artigo Instituição/País de afiliação: Instituto Butantan/BR
Buscar no Google
Coleções: Bases de dados nacionais / Brasil Base de dados: Sec. Est. Saúde SP / SESSP-IBACERVO / SESSP-IBPROD Assunto principal: Células / Fibroblastos Idioma: Inglês Revista: Photomedicine and laser surgery Ano de publicação: 2010 Tipo de documento: Artigo Instituição/País de afiliação: Instituto Butantan/BR
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