Isolation of the first IMP-4 metallo beta lactamase producing Klebsiella pneumoniae in Tianjin, China
Braz. j. microbiol
; 43(3): 917-922, July-Sept. 2012. ilus, tab
Article
em En
| LILACS
| ID: lil-656653
Biblioteca responsável:
BR32.1
ABSTRACT
This study shows for the first time the mechanism of carbapenem resistance of a Klebsiella pneumoniae clinical isolate TJ8 recovered from Tianjin medical university general hospital ,China. The modified Hodge test and EDTA synergy test were performed for the screening of carbapenemases and metallo-β-lactamases (MBLs), respectively. Polymerase chain reactions and DNA sequencing confirmed that the strain carried IMP-4 metallo-β-lactamase (MBL) , SHV-11 and TEM-1 β-lactamase. ClassⅠintegron was positive and gave a 3.0-kb PCR amplicon .IMP-4 was located in ClassⅠintegron 5'CS. The gene determinants were organized in the order of blaIMP-4-orfII-orfIII.In all, the results show that IMP-4 MBL production caused the TJ8 resistance to carbapenems.
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Texto completo:
1
Base de dados:
LILACS
Assunto principal:
Técnicas In Vitro
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Resistência Microbiana a Medicamentos
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Sequência de Bases
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Carbapenêmicos
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Reação em Cadeia da Polimerase
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Integrons
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Metaloproteases
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Suscetibilidade a Doenças
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Klebsiella pneumoniae
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article