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Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting
Gallo, Juliana Failde; Pinhata, Juliana Maira Watanabe; Chimara, Erica; Gonçalves, Maria Gisele; Fukasawa, Lucila Okuyama; Oliveira, Rosangela Siqueira de.
Afiliação
  • Gallo, Juliana Failde; Instituto Adolfo Lutz. Centro de Bacteriologia. Núcleo de Tuberculose e Micobacterioses. São Paulo. BR
  • Pinhata, Juliana Maira Watanabe; Instituto Adolfo Lutz. Centro de Bacteriologia. Núcleo de Tuberculose e Micobacterioses. São Paulo. BR
  • Chimara, Erica; Instituto Adolfo Lutz. Centro de Bacteriologia. Núcleo de Tuberculose e Micobacterioses. São Paulo. BR
  • Gonçalves, Maria Gisele; Instituto Adolfo Lutz. Centro de Bacteriologia. Núcleo de Tuberculose e Micobacterioses. São Paulo. BR
  • Fukasawa, Lucila Okuyama; Instituto Adolfo Lutz. Centro de Bacteriologia. Núcleo de Tuberculose e Micobacterioses. São Paulo. BR
  • Oliveira, Rosangela Siqueira de; Instituto Adolfo Lutz. Centro de Bacteriologia. Núcleo de Tuberculose e Micobacterioses. São Paulo. BR
Mem. Inst. Oswaldo Cruz ; 111(9): 545-550, Sept. 2016. tab
Article em En | LILACS | ID: lil-794728
Biblioteca responsável: BR1.1
ABSTRACT
Abstract Brazil is one of the high burden countries for tuberculosis, and a rapid diagnosis is essential for effective control of the disease. In the present study, an in-house real-time polymerase chain reaction (PCR) assay targeting the mpt64 gene for identification of Mycobacterium tuberculosis complex isolates was evaluated under routine diagnosis conditions in a reference laboratory. From May 2011 to July 2012, 1,520 isolates of mycobacteria were prospectively submitted for phenotypic and/or PRA-hsp65 identification and to real-time PCR. The mpt64 real-time PCR showed 99.7% sensitivity and 96% specificity and detected 79.4% of the cases missed by phenotypic and PRA-hsp65 identification. The in-house real-time PCR assay showed high sensitivity and specificity and was successfully implemented in the routine diagnosis of tuberculosis in a reference laboratory from a high burden setting.
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Texto completo: 1 Base de dados: LILACS Assunto principal: Tuberculose / Mycobacterium tuberculosis / Antígenos de Bactérias Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: LILACS Assunto principal: Tuberculose / Mycobacterium tuberculosis / Antígenos de Bactérias Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article