A ChromoQuant QF-PCR kit containing 24 primer pairs targeting loci on chromosomes 21, 13, 18, X and Y was employed to identify aneuploidies of the referred chromosomes.
QF-PCR was demonstrated to be efficient and reliable for prenatal aneuploidyscreening. This study suggests that QF-PCR can be used as a rapid diagnosticmethod. However, rearrangements and some mosaic samples cannot be detected with this test; thus, those exceptions must undergo cytogenetic analysis.