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Use of sodC versus ctrA for real-time polymerase chain reaction-based detection of Neisseria meningitidis in sterile body fluids.

Higa, Fábio Takenori; Fukasawa, Lucila Okuyama; Gonçalves, Maria Gisele; Salgado, Maristela Marques; Lemos, Ana Paula Silva de; Harrison, Lee H; Oliveira, Priscilla Lima de; Silva, Carla Naufal da; Sacchi, Claudio Tavares.
Mem Inst Oswaldo Cruz; 108(2): 246-7, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23579808
We evaluated the use of a newly described sodC-based real-time-polymerase chain reaction (RT-PCR) assay for detecting Neisseria meningitidis in normally sterile sites, such as cerebrospinal fluid and serum. The sodC-based RT-PCR assay has an advantage over ctrA for detecting nongroupable N. meningitidis isolates, which are commonly present in asymptomatic pharyngeal carriage. However, in our study, sodC-based RT-PCR was 7.5% less sensitive than ctrA. Given the public health impact of possible false-negative results due to the use of the sodC target gene alone, sodC-based RT-PCR for the diagnosis of meningococcal meningitis should be used with caution.