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[Functionality of Metdi5511gene in Methylobacterium dichloromethanicum DM4].

Firsova, Yu E; Torgonskaya, M L; Trotsenko, Yu A.
Prikl Biokhim Mikrobiol; 53(2): 196-203, 2017 Mar-Apr.
Artigo em Russo | MEDLINE | ID: mdl-29508981
A knockout mutant of Methylobacterium dichloromethanicum DM4 with an inactivated gene of a putative transcription regulator METDI5511 (ΔMETDI5511) has been obtained. The expression of this gene increases many times when the strain is grown on dichloromethane compared to methanol. The mutant had a low growth rate on dichloromethane as compared with the original strain and was found to be more sensitive to influences of various types of stress (oxidative, osmotic stress, heat, and drying). The cel