Lipid nanoparticle mediated small interfering RNA delivery as a potential therapy for Alzheimer's disease.

Alzheimer's disease (AD) is a neurodegenerative condition that exhibits a gradual decline in cognitive function and is prevalent among a significant number of individuals globally. The use of small interfering RNA (siRNA) molecules in RNA interference (RNAi) presents a promising therapeutic strategy for AD. Lipid nanoparticles (LNPs) have been developed as a delivery vehicle for siRNA, which can selectively suppress target genes, by enhancing cellular uptake and safeguarding siRNA from degradation. Numerous research studies have exhibited the effectiveness of LNP-mediated siRNA delivery in reducing amyloid beta (Aß) levels and enhancing cognitive function in animal models of AD. The feasibility of employing LNP-mediated siRNA delivery as a therapeutic approach for AD is emphasized by the encouraging outcomes reported in clinical studies for other medical conditions. The use of LNP-mediated siRNA delivery has emerged as a promising strategy to slow down or even reverse the progression of AD by targeting the synthesis of tau phosphorylation and other genes linked to the condition. Improvement of the delivery mechanism and determination of the most suitable siRNA targets are crucial for the efficacious management of AD. This review focuses on the delivery of siRNA through LNPs as a promising therapeutic strategy for AD, based on the available literature.

Bioenergy-producing two-stage septic tank and floating wetland for onsite wastewater treatment: Circuit connection and external aeration.

This study designed a two-stage, electrode-integrated septic tank-floating wetland system and assessed their pollutant removal performances under variable operational conditions. The two-stage system achieved mean organic, nitrogen, phosphorus, and coliform removal percentages of 99, 78, 99, and 97%, respectively, throughout the experimental run. The mean metals (chromium, cadmium, nickel, copper, zinc, lead, iron, and manganese) removal percentages ranged between 81 and 98%. Accumulated sludge, filler media, and the hanging root mass contributed to pollutant removals by supporting physicochemical and biological pathways. The mean effluent organic concentration and coliform number across the two-stage system were 20 mg/L and 1682 CFU/100 mL, respectively, during the closed-circuit protocol, which was beneath the open-circuit-based performance profiles, i.e., 32 mg/L and 2860 CFU/100 mL, respectively. Effluent organic, nitrogen, phosphorus, metals, and coliform number ranges across the two-stage system were 9-17 mg/L, 13-24 mg/L, 1-1.5 mg/L, 0.001-0.2 mg/L, and 1410-2270 CFU/100 mL, respectively during intermittent and continuous aeration periods. The air supply rate differences influenced pollutant removal depending on the associated removal mechanisms. The non-aeration phase produced higher effluent pollutant concentrations than the aeration periods-based profiles. The overall mean power density production of the septic tank ranged between 107 and 596 mW/m3; 110 and 355 mW/m3 with the floating wetland. The bioenergy production capacity of the septic tank was positively correlated to external air supply rates. This study demonstrates the potential application of the novel bioenergy-producing septic tank-floating wetland system for wastewater treatment in decentralized areas.

Recent advances in Zr and Hf-based MXenes and their hetero-structure as novel anode materials for Ca-ion batteries: theoretical insights from DFT approach.

Recently, MXenes have been widely investigated for use as electrodes in various ion storage batteries. In this study, Zr2N, Hf2N and ZrHfN were explored as potential anode materials for Ca-ion batteries. AIMD simulations predict higher structural stability for our proposed MXenes at a temperature of 300 K. The adsorption energies at the most favourable adsorption sites are 1.31, 1.33 and 1.27 eV for Zr2N, Hf2N and ZrHfN, respectively. During the adsorption process, a significant amount of charge transfer occurs from the Ca atom to the nanosheets. DOS and PDOS analyses reveal that the adsorption of Ca atoms enhances the conductivity of the nanosheets. Moreover, the low diffusion barriers are found to be 0.076, 0.073 and 0.097 eV when the Ca atom migrates from its favourable adsorption site to a nearby site on Zr2N, Hf2N and ZrHfN nanosheets, resulting in high charging rates. The theoretical capacities of Zr2N, Hf2N and ZrHfN nanosheets are 1034, 561 and 707 mA h g-1, respectively. All the results from this study suggest that our proposed nanosheets can be potential anode materials for Ca-ion batteries. Among them, the Zr2N nanosheet shows superior anodic properties for Ca-ion batteries, which is also confirmed by specific capacity, diffusion barrier and open circuit voltage calculations.

Challenges in the profitability of small-scale broiler farming by avoiding injudicious use of drugs and additives.

The aim of the current study was to determine the present scenario of injudicious use of drugs and additives in small-scale broiler operations and whether broilers can be produced successfully and profitably without the injudicious use of drugs and additives. First, a survey was undertaken in relation to farmers' basic information and general management methods in commercial broilers, with special attention given to the usage of medications and additives in drinking water. Second, based on the survey data, an experimental trial was carried out to compare the growth performance and economic profitability of rearing broilers with and without the use of said drugs and additives. A total of 540 broiler DOCs were allotted into three treatments: T1 = self-formulated feed (SFF) with judicious use of drugs and additives; T2 = commercial feed with judicious use of drugs and additives (JUDA) and T3 = commercial feed with injudicious use of drugs and additives (InJUDA), with six replications (30 birds/replication) in each. The results showed that the farmers used a variety of drugs and additives in 35 days of broiler rearing; however, the farmers usually did not consult with veterinary practitioners, instead relying on and being instructed by local dealers and medicine company representatives. Although the medications and additives account for almost 6-8% of total production costs, the experimental trial clearly demonstrated that the broilers kept with either JUDA or InJUDA showed statistically (p < 0.05) similar BW (2181.93 g & 2222.53 g/bird), BWG (2110.0 g & 2129.91 g/bird), and FCR (1.62 & 1.57, respectively), whereas broilers in the SFF group showed significantly lower growth performances (BW = 1799.31 g/bird, BWG = 1746.19 g/bird, and FCR = 1.93, respectively). The net profit per kg bird in the JUDA group was substantially (p < 0.05) greater (BDT- 27.34/-), followed by the SFF group (BDT- 25.56/) and the InJUDA group (BDT- 24.49/-). Taken together, these findings suggest that profitable broiler farming is possible without the injudicious use of drugs and additives.

Profitability and meat quality of fast-, medium- and slow-growing meat-type chicken genotypes as affected by growth and length of rearing.

The study aimed to evaluate the profitability, meat quality, and carcass parameters of fast-, medium-, and slow-growing meat-type chicken genotypes of Bangladesh. Nine hundred DOCs were randomly allocated to 6 treatments: T1 = commercial broilers, T2 = CPF-3 (central poultry farm-3), T3 = cockerel, T4 = sonali, T5 = NDD (non-descriptive desi), and T6 = hilly, having 5 replications of 30 chicks each. Birds were reared under complete confinement until their respective market ages (commercial broilers = 35 d; CPF-3 = 45 d; cockerel = 56 d, and hilly = 77 d; sonali = 63 d and NDD = 77 d) and fed commercial broiler diets. Net returns, meat quality, growth, and carcass yield were measured. NDD and hilly showed significantly the highest profitability and superior meat quality. Commercial broilers exhibited the highest final body weight (2355.59 g/b) followed by hilly (1241 g/b) and NDD (1006 g/b), while CPF-3 (860.21 g/b), cockerel (915.49 g/b), and sonali (788.43 g/b) had lower final body weights at their respective market ages. Commercial broilers had the highest carcass weight and dressing yields, followed by hilly and cockerel, and lower in sonali, CPF-3, and NDD. The study concluded that rearing slow- or medium-growing NDD and hilly is superior to fast-growing commercial broilers or CPF-3 regarding profitability, and meat quality. The results of current findings help small-scale farmers in choosing a suitable meat-type chicken that yields better profitability and also for the consumers who wish to pay a fair price for the birds, considering the meat quality specific to each chicken genotype.

Assessment of the drug delivery potential of graphene, boron nitride and their in-plane doped structures for hydroxyurea anti-cancer drug via DFT study.

Globally, cancer is the most common cause of mortality among all deadly diseases. As a result, a nanotechnology-based drug delivery system is used to improve the efficacy of cancer treatment, which provides an improved therapeutic index and delivers multiple drugs directly to the tumor site. In the present work, DFT calculations were employed to investigate the surface adsorption of a hydroxyurea (HU) anticancer drug on pristine graphene (GP), boron nitride (B24N24), and doped GP by replacing some of its carbon atoms with boron (B) and nitrogen (N) atoms to form C30B9N9, C16B16N16, and C6B21N21 nanosheets. In gas media, HU is adsorbed on these C30B9N9, C16B16N16, C6B21N21, and B24N24 nanosheets with adsorption energies of -0.70, -3.03, -2.47, and -1.96 eV, respectively. Alternatively, in water solvent media, the adsorption energies of C30B9N9, C16B16N16, C6B21N21, and B24N24 are -0.82, -0.29, -0.15, and -0.26 eV, respectively. The energy gaps of the nanosheets were found to be 0.288, 0.174, 0.14, and 4.562 eV before adsorption, respectively. After the adsorption of HU on the proposed nanosheets, the energy gap was reduced to 0.15 eV for C16B16N16. According to the DOS spectra, noticeable peaks appeared in the Fermi level after the adsorption of HU on the nanosheets, which indicates the reduction of the energy gap. Quantum molecular analysis predicted that the chemical potential, electrophilicity index, and nucleophilicity index of C16B16N16 increased, whereas global hardness decreased, indicating high reactivity. Therefore, it can be concluded that among the proposed nanosheets, C16B16N16 would be an appropriate carrier for the HU drug.

XPS valence band observable light-responsive system for photocatalytic acid Red114 dye decomposition using a ZnO-Cu2O heterojunction.

ZnO-Cu2O composites were made as photocatalysts in a range of different amounts using an easy, cheap, and environment-friendly coprecipitation method due to their superior visible light activity to remove pollutants from the surrounding atmosphere. X-ray diffraction and Fourier transform infrared spectroscopy (FT-IR) have demonstrated that ZnO-Cu2O catalysts are made of highly pure hexagonal ZnO and cubic Cu2O. X-ray photoelectron spectroscopy has confirmed that there is a substantial interaction between the two phases of the resultant catalyst. The optical characterizations of the synthesized ZnO-Cu2O composite were done via UV-vis reflectance spectroscopy. Due to the doping on ZnO, the absorption range of the ZnO-Cu2O catalyst is shifted from the ultraviolet to the visible region due to diffuse reflection. The degradation efficiency is affected by the Ratio of ZnO: Cu2O and ZnO-Cu2O composite with a proportion of 90:10 exhibited the most prominent photocatalytic activity on Acid Red 114, with a pseudo-first-order rate constant of 0.05032 min-1 that was 6 and 11 times greater than those of ZnO and Cu2O, respectively. The maximum degradation efficiency is 97 %. The enhanced photocatalytic activity of the composite is caused by the synergistic interaction of ZnO and Cu2O, which improves visible light absorption by lowering band gap energy and decreasing the rate at which the electron-hole pairs recombine. The scavenging experiment confirmed that hydroxyl radical was the dominant species for the photodegradation of Acid Red 114. Notably, the recycling test demonstrated the ZnO-Cu2O photocatalyst was highly stable and recyclable. These results suggest that the ZnO-Cu2O mix might be able to clean up environmental pollutants when it meets visible light.

A prospective review of the health-promoting potential of Jing Si Herbal Tea.

Traditional Chinese medicine (TCM) has gained considerable attention over the past few years for its multicomponent, multitarget, and multi-pathway approach to treating different diseases. Studies have shown that TCMs as adjuvant therapy along with conventional treatment may benefit in safely treating various disorders. However, investigations on finding effective herbal combinations are ongoing. A novel TCM formula, "Jing Si Herbal Tea (JSHT)," has been reported recently for their health-promoting effects in improving overall body and mental health. JSHT is a combination of eight herbs recognized in Chinese herbal pharmacopoeia for their anti-viral, anti-aging, and anti-cancer properties as well as protective effects against cardiovascular, metabolic, neural, digestive, and genitourinary diseases. Thus, to better understand the beneficial effects of the ingredients of JSHT on health, this review intends to summarize the preclinical and clinical studies of the ingredients of JSHT on human health and diseases, and possible therapeutic effects with the related mode of actions and future prospects for their application in complementary therapies.

Institutional capacity assessment in the lens of implementation research: Capacity of the local institutions in delivering WASH services at Cox's Bazar district, Bangladesh.

BACKGROUND: The influx of Forcibly Displaced Myanmar Nationals (FDMNs) has left the Southwest coastal district of Cox's Bazar with one of the greatest contemporary humanitarian crises, stressing the existing water, sanitation, and hygiene (WASH) resources and services. This study aimed to assess the existing capacity of local institutions involved in delivering WASH services and identify relevant recommendations for intervention strategies. METHODS: We used a qualitative approach, including interviews and capacity assessment workshops with institutions engaged in WASH service delivery. We conducted five key informant interviews (KII) with sub-district level officials of the Department of Public Health Engineering (DPHE), Directorate General of Health Services (DGHS), Directorate of Primary Education (DPE) and Bangladesh Rural Advancement Committee (BRAC) to have a general idea of WASH service mechanisms. Seven capacity assessment workshops were organized with the relevant district and sub-district level stakeholders from August 2019 to September 2019. These workshops followed three key areas: i) knowledge of policy, organizational strategy, guidelines, and framework; ii) institutional arrangements for service delivery such as planning, implementation, coordination, monitoring, and reporting; and iii) availability and management of human, financial and infrastructural resources. Data were categorized using thematic content analysis. RESULTS: The majority of stakeholders lacked awareness of national WASH policies. Furthermore, the top-down planning approaches resulted in activities that were not context-specific, and lack of coordination between multiple institutions compromised the optimal WASH service delivery at the local level. Shortage of human resources in delivering sustainable WASH services, inadequate supervision, and inadequate evaluation of activities also required further improvement, as identified by WASH stakeholders. CONCLUSION: Research evidence suggests that decision-makers, donors, and development partners should consider learning from the WASH implementers and stakeholders about their existing capacity, gaps, and opportunities before planning for any WASH intervention in any particular area.

Restoring adiponectin via rosiglitazone ameliorates tissue wasting in mice with lung cancer.

AIM: To investigate systemic regulators of the cancer-associated cachexia syndrome (CACS) in a pre-clinical model for lung cancer with the goal to identify therapeutic targets for tissue wasting. METHODS: Using the Kras/Lkb1 (KL) mouse model, we found that CACS is associated with white adipose tissue (WAT) dysfunction that directly affects skeletal muscle homeostasis. WAT transcriptomes showed evidence of reduced adipogenesis, and, in agreement, we found low levels of circulating adiponectin. To preserve adipogenesis and restore adiponectin levels, we treated mice with the PPAR-γ agonist, rosiglitazone. RESULTS: Rosiglitazone treatment increased serum adiponectin levels, delayed weight loss, and preserved skeletal muscle and adipose tissue mass, as compared to vehicle-treated mice. The preservation of muscle mass with rosiglitazone was associated with increases in AMPK and AKT activity. Similarly, activation of the adiponectin receptors in muscle cells increased AMPK activity, anabolic signaling, and protein synthesis. CONCLUSION: Our data suggest that PPAR-γ agonists may be a useful adjuvant therapy to preserve tissue mass in lung cancer.

Evaluation of occupational health and safety intervention for the waste and sanitation workers in Bangladesh during COVID-19.

Waste and sanitation workers in South-Asian countries are vulnerable to injuries and diseases, including COVID-19. In Bangladesh, an intervention was implemented during COVID-19 to lower these workers' occupational health risks through training and PPE distribution. We assessed how the intervention affected their occupational health behaviors using a randomized cluster trial in 10 Bangladeshi cities, including seven intervention and three control areas. We conducted 499 surveys (Control-152, Intervention-347) and 47 structured observations (Control:15, Intervention:32) at baseline and 499 surveys (Control:150, Intervention:349) and 50 structured observations (Control:15, Intervention:35) at endline. To evaluate the impact of intervention at the endline, we used the difference in difference (DID) method. Compared to control, workers from intervention areas were more likely to have increased knowledge of using/maintaining PPEs (adjusted DID: 21%, CI: 8, 33), major COVID-19 transmission causes (adjusted DID: 27%, CI: 14, 40), and preventive measures (adjusted DID: 12%, CI: -0.6, 25), and improved attitude about using PPEs (adjusted DID: 36%, CI: 22, 49), washing PPEs (adjusted DID: 20%, CI: 8, 32). The intervention was more likely to improve workers' self-reported practice of taking adequate precautions after getting back from work (adjusted DID: 37%, CI: 27, 47) and changing/cleaning masks every day (adjusted DID: 47%, CI: 0.03, 94), and observed practices of maintaining coughing etiquette (DID: 20%, CI: 0.2, 40) at workplace and handwashing before wearing PPEs (DID: 27%, CI: 2, 52), after finishing work (DID: 31%, CI: -7, 69) & during work intervals (DID: 30%, CI: -33, 93). There was not much improvement in observed practices of mask use (DID: 1%, CI: -40, 42), handwashing before meals (DID: 2%, CI: -61, 65), and after toilet (DID: 7%, CI: -41, 55). This intervention has improved the knowledge, attitude and practice of the workers about critical occupational risk mitigation, which may be replicated in similar settings. Future interventions need to address occupational health-related injuries and health complications, introduce regular health checkups/insurance for the workers, create a balance between the quality and comfort of the PPEs and ensure a mechanism to ensure a regular supply of PPEs.

Using publicly available, interactive epidemiological dashboards: an innovative approach to sharing data from the Rakai Community Cohort Study.

Objectives: Public sharing of de-identified biomedical data promotes collaboration between researchers and accelerates the development of disease prevention and treatment strategies. However, open-access data sharing presents challenges to researchers who need to protect the privacy of study participants, ensure that data are used appropriately, and acknowledge the inputs of all involved researchers. This article presents an approach to data sharing which addresses the above challenges by using a publicly available dashboard with de-identified, aggregated participant data from a large HIV surveillance cohort. Materials and Methods: Data in this study originated from the Rakai Community Cohort Study (RCCS), which was integrated into a centralized data mart as part of a larger data management strategy for the Rakai Health Sciences Program in Uganda. These data were used to build a publicly available, protected health information (PHI)-secured visualization dashboard for general research use. Results: Using two unique case studies, we demonstrate the capability of the dashboard to generate the following hypotheses: firstly, that HIV prevention strategies ART and circumcision have differing levels of impact depending on the marital status of investigated communities; secondly, that ART is very successful in comparison to circumcision as an interventional strategy in certain communities. Discussion: The democratization of large-scale anonymized epidemiological data using public-facing dashboards has multiple benefits, including facilitated exploration of research data and increased reproducibility of research findings. Conclusion: By allowing the public to explore data in depth and form new hypotheses, public-facing dashboard platforms have significant potential to generate new relationships and collaborations and further scientific discovery and reproducibility.

A model for the noninvasive, habitat-inclusive estimation of upper limit abundance for synanthropes, exemplified by M. fascicularis.

Accurately estimating population sizes for free-ranging animals through noninvasive methods, such as camera trap images, remains particularly limited by small datasets. To overcome this, we developed a flexible model for estimating upper limit populations and exemplified it by studying a group-living synanthrope, the long-tailed macaque (Macaca fascicularis). Habitat preference maps, based on environmental and GPS data, were generated with a maximum entropy model and combined with data obtained from camera traps, line transect distance sampling, and direct sightings to produce an expected number of individuals. The mapping between habitat preference and number of individuals was optimized through a tunable parameter ρ (inquisitiveness) that accounts for repeated observations of individuals. Benchmarking against published data highlights the high accuracy of the model. Overall, this approach combines citizen science with scientific observations and reveals the long-tailed macaque populations to be (up to 80%) smaller than expected. The model's flexibility makes it suitable for many species, providing a scalable, noninvasive tool for wildlife conservation.

Biomaterial-based in vitro 3D modeling of glioblastoma multiforme.

Adult-onset brain cancers, such as glioblastomas, are particularly lethal. People with glioblastoma multiforme (GBM) do not anticipate living for more than 15 months if there is no cure. The results of conventional treatments over the past 20 years have been underwhelming. Tumor aggressiveness, location, and lack of systemic therapies that can penetrate the blood-brain barrier are all contributing factors. For GBM treatments that appear promising in preclinical studies, there is a considerable rate of failure in phase I and II clinical trials. Unfortunately, access becomes impossible due to the intricate architecture of tumors. In vitro, bioengineered cancer models are currently being used by researchers to study disease development, test novel therapies, and advance specialized medications. Many different techniques for creating in vitro systems have arisen over the past few decades due to developments in cellular and tissue engineering. Later-stage research may yield better results if in vitro models that resemble brain tissue and the blood-brain barrier are used. With the use of 3D preclinical models made available by biomaterials, researchers have discovered that it is possible to overcome these limitations. Innovative in vitro models for the treatment of GBM are possible using biomaterials and novel drug carriers. This review discusses the benefits and drawbacks of 3D in vitro glioblastoma modeling systems.

Masturbation in a male Phayre's langur, Trachypithecus phayrei.

Masturbation is a common auto-sexual behaviour in humans but is not explicitly known in a major portion of non-human primates. We report the first masturbatory behaviour in a male Phayre's langur, Trachypithecus phayrei, observed in a semi-evergreen forest in northeastern Bangladesh. Like other Asian colobines, the multi-male-multi-female groups of the Phayre's langur suggest a multilevel social organization and a complex hierarchy among males. The lack of sexual opportunity could result in masturbation and sperm competition among males. However, sperm competition is reported to be low in such non-seasonal breeders. Hence, we suggest an adequate characterization of the socio-sexual behaviour and reproductive strategies of this globally endangered primate in order to demonstrate the causes, cost and consequences of masturbation. We also urge further scientific exploration into masturbation among primates due to its evolutionary and conservational significance.