School-Based Circuit Training Intervention Improves Local Muscular Endurance in Primary School Students: A Randomized Controlled Trial.

BACKGROUND: This study aimed to explore the effectiveness of a 12-week circuit training program in improving local muscular endurance in normal-weighted primary school students. METHODS: The study involved a parallel-group randomized trial with 606 primary school boys assigned to an experimental or a control group. The participants underwent a 12-week circuit training program that involved multi-joint, total-body workouts using body weight, resistance bands, and medicine ball exercises. The study measured the participants' local muscular endurance during sit-ups, dynamic trunk extensions on a Roman bench (DTE), and push-ups. RESULTS: After adjusting for the baseline, the treatment-grade interaction was significant for sit-ups (F = 7.74, p < 0.001, ηp2 = 0.04), DTE (F = 6.49, p < 0.001, ηp2 = 0.03), and push-ups (F = 9.22, p < 0.001, ηp2 = 0.05), where the experimental treatment was more beneficial than the control. The treatment effect seemed to vary depending on the individual's baseline local muscle endurance capacity. As the baseline local muscular endurance values increased, the treatment and grade effects became less beneficial. CONCLUSION: A 12-week circuit training program involving body weight, resistance bands, and medicine ball exercises suits school-based programs and can improve local muscular endurance in normal-weighted primary school boys. The experimental treatment was more effective than the control, and the individual baseline muscular endurance should be considered when designing training programs.

Differences between Healthy-Weight and Overweight Serbian Preschool Children in Motor and Cognitive Abilities.

The aim of this study was to determine the differences between healthy-weight and overweight 5-6-year-old preschool children in fine and gross motor skills and cognitive abilities. There were 91 subjects, preschool children (41 boys and 50 girls), who participated in this cross-sectional study. The body mass index (BMI) was calculated based on measures of body height and body mass, and WHO cutoff points were used for the assessment of the children's nutrition status. Fine motor abilities were determined using two Bruininks-Oseretsky (BOT-2) subtests, and gross motor skills are determined by the Test of Gross Motor Development (TGMD-2), while cognitive abilities were tested by the School Maturity Test (TZS+). Based on an independent-samples t-test, a difference in two out of three variables of gross motor skills was determined: manipulative skills and total gross motor skills between healthy-weight and overweight children, while in fine motor abilities and cognitive abilities there was no difference between these two groups. Although significant differences were found only in gross motor skills between healthy and overweight preschool children but not in fine motor skills and cognitive abilities, further longitudinal studies are required to understand the mechanisms of this, including the possible role of psychological factors.

Emotional Reactivity and Emotion Regulation Among Young Adults During COVID-19 Lockdown: The Moderating Role of Gender and Engagement in Sports.

The effects of the COVID-19 pandemic on mental health have not been fully inspected among the young adults' population. The objectives of the present study were: (1) to examine differences in emotional reactivity and emotion regulation between, both gender and sports engagement level during the first 2 weeks of the lockdown; and (2) to examine the possible impact of emotion regulation on emotional reactivity, and possible significant roles of gender and sports engagement level as moderators. This cross-sectional study included 315 Serbian young adults (aged 18-26 years old) during COVID-19 lockdown. Respondents answered socio-demographic questions and the Serbian version of the Multidimensional Emotion Questionnaire (MEQ). The results of confirmatory factor analysis indicated good fit for both positive and negative reactivity scales (SRMR = 0.037; CFI = 0.984, RMSEA = 0.046, and SRMR = 0.055; CFI = 0.964, RMSEA = 0.064, respectively). Gender differences were found in both positive (p = 0.039; d = 0.28) and negative emotional reactivity scales (p < 0.001; d = 0.60), with females reported lower and higher values, respectively. Professional athletes presented higher scores in positive reactivity scale in comparison to non-athletes (p < 0.001; d = 0.78) and recreational athletes (p = 0.034; d = 0.34) during 2 weeks of COVID-19 lockdown. Conversely, professional athletes scored lower in negative emotional reactivity scale in comparison to non-athletes (p < 0.001; d = 0.85) and recreational athletes (p = 0.006; d = 0.42). Both gender and sports engagement level differences were found for negative, but not for positive emotion regulation scale. Furthermore, results showed that engagement in sports level plays a significant role as moderator in relationship between negative regulation and negative reactivity, where professional athletes presented significant interaction effect and predicted lower negative reactivity scores compared to non-athletes and recreational athletes. However, gender does not moderate the influence of emotion regulation on emotional reactivity either positive or negative. Engagement in sports as a lifestyle may contribute to better emotional harmony especially in the crisis situation as COVID-19 lockdown.

Secondary hemophagocytic lymphohistiocytosis in a child with Leptospira infection: A case report.

Jevtic D, Djokic D, Redzic D, Aleksic D, Parezanovic M, Pasic S. Secondary hemophagocytic lymphohistiocytosis in a child with Leptospira infection: A case report. Turk J Pediatr 2018; 60: 735-738. Leptospirosis caused by spirochetes of the genus Leptospira in most patients result in very mild illness without jaundice. However, a small portion of patients develop various complications due to the involvement of multiple organ systems. Hemophagocytic lymphohistiocytosis is characterized by prolonged fever, hepatosplenomegaly and cytopenias, hyperferritinemia and hypertriglyceridemias, hyperfibrinogenemia, and hemophagocytosis in bone marrow, lymph nodes, spleen, or liver. Hemophagocytic lymphohistiocytosis associated with leptospirosis is a very rare condition and it should be considered in patients with multiple organ dysfunctions, together with adequate laboratory findings. It can delay the correct diagnosis of leptospirosis and contribute to an adverse outcome. We present a 13-year-old girl with secondary hemophagocytic lymphohistiocytosis caused by leptospira infection and favorable outcome with appropriate antibiotics and corticosteroid therapy.

Risk factors for severe dental anxiety among medical students.

BACKGROUND/AIM: Severe dental anxiety (SDA) is the most severe form of dental anxiety, thus the aim of this study was to determine the factors associated with SDA in students of health-related disciplines. METHODS: In this case-control study the cases were students with severe dental anxiety. The study was conducted at the Faculty of Medical Sciences, University of Kragujevac, Serbia. The participants were undergraduate students attending lectures during spring semester 2010/2011 (n = 1,812). A random sample of 800 students was assessed for the association between various risk factors and the severe dental anxiety. The main outcome measures were the data on demographics, dental anxiety, habits concerning oral hygiene, nutrition, general anxiety and (co)morbidity which were collected from the study participants by semi-structured questionnaire. RESULTS: Less frequent visits to the dentist (OR adjusted = 7.02 [2.65; 18.60]) and visiting the dentist only when there is a dental problem (OR adjusted = 8.08 [1.28; 50.93]) were associated with severe dental anxiety. The same was true for improper oral hygiene (OR adjusted = 4.25 [1.16; 15.60]). Factors as changing toothbrush more frequently (OR adjusted = 0.33 [0.14; 0.76]) and having chronic disease (OR adjusted = 0.01 [0.00; 0.09]) were inversely associated with severe dental anxiety. The level of education of students was not associated with severe dental anxiety. CONCLUSION: Inappropriate oral hygiene, less frequent changes of a toothbrush and less frequent visits to the dentist are important risk factors for severe dental anxiety.

[Preparation and testing of solutions for organ perfusion and preservation in transplantation].

BACKGROUND/AIM: Histidine-tryptophan-ketoglutarate (HTK) solution is the most frequently used solution for the preservation of abdominal organs by leading transplant centers worldwide. The aim of the study was to investigate whether it is possible to prepare this kind of perfusion solution in the hospital pharmacy. In the hospital pharmacy setting we prepared HTK preservation solution and tested its quality, and in accordance with the obtained results recommended its shelf-life. METHODS: A multicomponent HTK solution was prepared following standardized aseptic procedures for sterile products. Components of HTK solution were dissolved in water for injection under aseptic conditions and the obtained solution was sterilized by bacteriological membrane filtration (0.22 microm). The quality of the produced solution was examined using physical and physico-chemical methods and biological tests. Following pharmacopoeial regulations, we examined clarity, pH value, sterility and performed testing on pyrogens. The content of the amino acids histidine and triptophan was determined by using high pressure liquid chromatography (HPLC) method. The concentrations of chloride ions were investigated by retitration method. Atomic absorption spectrometry and ion-exchange chromatography with conductometric detection were used for determining the level of magnesium and calcium ions and the concentrations of sodium and potassium ions in HTK solution, respectively. RESULT: It was shown that the quality of the prepared HTK solution complied with the regulation demands. Also, pH value of the solution as a function of storage temperature, remained in the physiological range (pH 7.4). CONCLUSION: According to the obtained results of physico-chemical and biological testing it can be concluded that in the hospital pharmacy setting with the applied technological procedure, we can produce HTK preservation solution of the required quality. Keeping of HTK preservation solution in a refrigerator at 4 degrees C warrants its shelf-life of six months.

[Stability investigation of total parenteral nutrition admixture prepared in a hospital pharmacy].

BACKGROUND/AIM: In the cases when nutrition of patients can not be orally nor enterally performed, parenteral nutrition is a method of the therapy that provides more successful and rapid recovery. In that way, hospitalization can be significantly shorter, healing costs reduced and mortality minimized. Total parenteral nutrition (TPN) admixtures are the most complex systems which contain amino acids, carbohydrates, lipid emulsion, macroelectrolytes (Na+, K+, Ca2+, Mg2+, Cl(-), SO4(2-), PO4(3-)), oligoelements, hydro- and liposoluble vitamines, heparin, insulin and water. Concerning the mentioned complexity, special attention should be payed to physicochemical and microbiological stability of a mixture, because of interactions among components, that can be very hard to analyze. The aim of this study was to investigate the problem of stability of TPN admixtures prepared in a hospital pharmacy. METHODS: Admixture TPN was aseptically prepared in laminar air--flow environment on the basis of the specified order in supplementing components and additives to basic solutions. Solutions were kept in sterile multicompartment ethylene-vinyl-acetate bags. After preparation and slow homogenization, TPN admixtures were submitted to physicochemical and microbiological stability analyses in various period of time. The assessment of physical stability of TPN admixture was done on the basis of visual inspection, determination of pH value and measuring of particle size. The investigation of sterility and pyrogenic test were performed according to Ph. Yug. V regulations. RESULTS: Physico-chemical and microbiological analyses were applied and no significant changes in visual sense, pH value and droplet size stability of the TPN admixture were observed during the period of 60 hours The lipid droplets were smaller in size than 5 microm, that is the most common pharmacopoeia requirement. CONCLUSION: The results of our study confirmed that a TPN admixture prepared in a hospital pharmacy can be stored without stability loss for at least 60 hours.