RESUMO
Alterations in dopaminergic transmission are associated with neurological disorders, such as depression, autism, and Parkinson's disease. Exposure of rats to ambient fine (FP) or ultrafine (UFP) particles induces oxidative and inflammatory responses in the striatum, a neuronal nucleus with dense dopaminergic innervation and critically involved in the control of motor activity.Objectives: We used an ex vivo system to evaluate the effect of in vivo inhalation exposure to FP and UFP on motor activity and dopaminergic transmission.Materials and Methods: Male adult Wistar rats were exposed to FP, UFP, or filtered air for 8 weeks (subchronic exposure; 5 h/day, 5 days/week) in a particle concentrator. Motor activity was evaluated using the open-field test. Uptake and release of [3H]-dopamine were assessed in striatal synaptosomes, and dopamine D2 receptor (D2R) affinity for dopamine was evaluated by the displacement of [3H]-spiperone binding to striatal membranes.Results: Exposure to FP or UFP significantly reduced spontaneous motor activity (ambulatory distance: FP -25%, UFP -32%; ambulatory time: FP -24%, UFP -22%; ambulatory episodes: FP -22%, UFP -30%), decreased [3H]-dopamine uptake (FP -18%, UFP -24%), and increased, although not significantly, [3H]-dopamine release (113.3 ± 16.3 and 138.6 ± 17.3%). Neither FP nor UFP exposure affected D2R density or affinity for dopamine.Conclusions: These results indicate that exposure to ambient particulate matter reduces locomotion in rats, which could be related to altered striatal dopaminergic transmission: UFP was more potent than FP. Our results contribute to the evidence linking environmental factors to changes in brain function that could turn into neurological and psychiatric disorders.HIGHLIGHTSYoung adult rats were exposed to fine (FP) or ultrafine (UFP) particles for 40 days.Exposure to FP or UFP reduced motor activity.Exposure to FP or UFP reduced dopamine uptake by striatal synaptosomes.Neither D2R density or affinity for dopamine was affected by FP or UFP.UFP was more potent than FP to exert the effects reported.
Assuntos
Poluentes Atmosféricos , Material Particulado , Ratos , Masculino , Animais , Material Particulado/toxicidade , Tamanho da Partícula , Dopamina , Ratos Wistar , Atividade Motora , Poluentes Atmosféricos/toxicidadeRESUMO
Wood smoke (WS) contains many harmful compounds, including polycyclic aromatic hydrocarbons (PAHs). WS induces inflammation in the airways and lungs and can lead to the development of various acute and chronic respiratory diseases. Pulmonary fibroblasts are the main cells involved in the remodeling of the extracellular matrix (ECM) during the WS-induced inflammatory response. Although fibroblasts remain in a low proliferation state under physiological conditions, they actively participate in ECM remodeling during the inflammatory response in pathophysiological states. Consequently, we used normal human lung fibroblasts (NHLFs) to assess the potential effects of the PAHs-containing wood smoke extract (WSE) on the growth rate, total collagen synthesis, and the expression levels of collagen I and III, matrix metalloproteinase (MMP)-1, MMP-2, MMP-9, tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, and the transforming growth factor (TGF)-ß1. We also assessed MMPs activity. The results showed that WSE induced a trimodal behavior in the growth rate curves in NHLFs; the growth rate increased with 0.5-1 % WSE and decreased with 2.5% WSE, without causing cell damage; 5-20% WSE inhibited the growth and induced cell damage. After 3 hours of exposure, 2.5% WSE induced an increase in total collagen synthesis and upregulation of TGF-ß1, collagen I and III, MMP-1, TIMP-1, and TIMP-2 expression. However, MMP-2 expression was downregulated and MMP-9 was not expressed. The gelatinase activity of MMP-2 was also increased. These results suggest that WSE affects the ECM remodeling in NHLFs and indicate the potential involvement of PAHs in this process.
Assuntos
Matriz Extracelular/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Inflamação/induzido quimicamente , Inflamação/fisiopatologia , Pneumopatias/induzido quimicamente , Extratos Vegetais/efeitos adversos , Fumaça/efeitos adversos , Proliferação de Células/efeitos dos fármacos , Humanos , Magnoliopsida/química , Madeira/químicaRESUMO
The Mexico City Metropolitan Area (MCMA) was the object of a chemical elemental characterization (Ti, V, Cr, Mn, Co, Ni, Cu, Mo, Ag, Cd, Sb, Pb, La, Sm, Ce, and Eu) of PM2.5 collected during 2013 and analyzed by inductively coupled plasma mass spectrometry (ICP-MS). Sampling campaigns were carried out at five locations simultaneously-northwest, northeast, center, southwest, and southeast-during dry-warm season (April), rainy season (August), and dry-cold season (November). By means of enrichment factor (EF) and principal component analysis (PCA), it was possible to attribute the analyzed elements to geogenic and anthropogenic sources, as well as to identify a group of elements with mixed provenance sources. The highest concentrations for most metals were found in northwest and northeast, and during dry-warm (DW), confirming the trend observed in PM2.5 samples collected in 2011. Despite similarities between 2011 and 2013, an increase of 17% in PM2.5 mass concentration was observed, mainly attributable to geogenic sources, whereby the importance of wind intensity to the impact of emission sources is highlighted. The effect of wind intensity was revealed, by means of polar plots, as the controlling mechanism for this increase. This allowed us to conclude that high-speed episodes (5 m s-1) were responsible for raising geogenic metal concentrations rather than wind direction.
Assuntos
Poluentes Atmosféricos/análise , Monitoramento Ambiental , Material Particulado/análise , Cidades , Metais/análise , México , Estações do Ano , VentoRESUMO
The study of airborne metals in urban areas is relevant due to their toxic effects on human health and organisms. In this study, we analyzed metals including rare earth elements (REE) in particles smaller than 2.5 µm (PM2.5), collected at five sites around the Mexico City Metropolitan Area (MCMA), during three periods in 2011: April (dry-warm season, DW), August (rainy season, R), and November (dry-cold season, DC). Principal component analysis allowed identifying factors related to geogenic sources and factors related to anthropogenic sources. The recognition of the high impact of geogenic sources in PM2.5 is in agreement with the REE distribution patterns, which show similar behavior as those shown by igneous rocks, confirming the influence of the regional geogenic material. Metals associated to geogenic sources showed higher concentration (p < 0.05) at NE of the MCMA and a significant correlation with prevalent winds. Geogenic metals show similar seasonal distribution, with the highest concentration during DW (p < 0.05), suggesting a possible metal resuspension effect which affects more significantly at lower relative humidity (RH). The metals associated with anthropogenic sources are in agreement with the urban complexity of the area, showing homogenous distribution throughout MCMA (p > 0.05) and no similar seasonal pattern among them. These unexpected results exposed outstanding information regarding the identification of different geogenic sources as the main contributors of metals in the atmospheric environment in the MCMA and highlighted the importance of meteorology in the spatial and seasonal metal patterns.
Assuntos
Poluentes Atmosféricos/análise , Monitoramento Ambiental , Metais/análise , Material Particulado/análise , Cidades , Humanos , México , Estações do Ano , VentoRESUMO
In this study, atmospheric mercury concentration in airborne particulate matter with an aerodynamic diameter ≤ 2.5 µm (PM2.5) was analyzed by ICP-MS. Samples were collected in the Mexico City Metropolitan Area (MCMA), during 2013, in five locations, Northwest, Northeast (NE), Central, Southwest and Southeast, along three seasons: dry warm, rainy, and dry cold (DC). It can be observed that NE shows the highest mercury concentration (p < 0.05), where pollution events were identified. The seasonal distribution shows that samples collected during DC present the highest concentration (p < 0.05). These results are in agreement with the distribution of important mercury industrial sources located in the northern urban area as well with the temperature and wind conditions during 2013. The comparison of data obtained in this work with those of similar previous studies clearly indicates a decrease, between 2006 and 2013, of mercury content in PM2.5 collected in MCMA.
Assuntos
Poluentes Atmosféricos/análise , Monitoramento Ambiental/métodos , Mercúrio/análise , Material Particulado/análise , Cidades , México , Tamanho da Partícula , Estações do Ano , VentoRESUMO
The increase in platinum (Pt) in the airborne particulate matter with size ≤2.5 µm (PM2.5) in urban environments may be interpreted as result of the abrasion and deterioration of automobile catalyst. Nowadays, about four million vehicles in Mexico City use catalytic converters, which means that their impact should be considered. In order to evaluate the contribution of Pt to environmental pollution of the metropolitan area of Mexico City (MAMC), airborne PM2.5 was collected at five different sites in the urban area (NW, NE, C, SW, SE) in 2011 during April (dry-warm season), August (rainy season) and December (dry-cold season). Analytical determinations were carried out using a ICP-MS with a collision cell and kinetic energy discrimination. The analytical and instrument performance was evaluated with standard road dust reference material (BCR-723). Median Pt concentration in the analyzed particulate was is 38.4 pg m(-3) (minimal value 1 pg m(-3) maximal value 79 pg m(-3)). Obtained Pt concentrations are higher than those reported for other urban areas. Spatial variation shows that SW had Pt concentration significantly higher than NW and C only. Seasonal variation shows that Pt median was higher in rainy season than in both dry seasons. A comparison of these results with previously reported data of PM10 from 1991 and 2003 in the same studied area shows a worrying increase in the concentration of Pt in the air environment of MAMC.
Assuntos
Poluentes Atmosféricos/análise , Monitoramento Ambiental , Material Particulado/análise , Platina/análise , Cidades , Poeira/análise , Espectrometria de Massas/métodos , México , Tamanho da Partícula , Estações do AnoRESUMO
The heterogeneity and complexity of solvent-extracted organic matter associated with PM2.5 (SEOM-PM2.5) is well known; however, there is scarce information on its biological effects in human cells. This work aimed to evaluate the effect of SEOM-PM2.5 collected in northern Mexico City during the cold-dry season (November 2017) on NL-20 cells, a human bronchial epithelial cell line. The SEOM obtained accounted for 15.5% of the PM2.5 mass and contained 21 polycyclic aromatic hydrocarbons (PAHs). The cell viability decreased following exposure to SEOM-PM2.5, and there were noticeable morphological changes such as increased cell size and the presence of cytoplasmic vesicles in cells treated with 5-40 µg/mL SEOM-PM2.5. Exposure to 5 µg/mL SEOM-PM2.5 led to several alterations compared with the control cells, including the induction of double-stranded DNA breaks based (p < 0.001); nuclear fragmentation and an increased mitotic index (p < 0.05); 53BP1 staining, a marker of DNA repair by non-homologous end-joining (p < 0.001); increased BiP protein expression; and reduced ATF6, IRE1α, and PERK gene expression. Conversely, when exposed to 40 µg/mL SEOM-PM2.5, the cells showed an increase in reactive oxygen species formation (p < 0.001), BiP protein expression (p < 0.05), and PERK gene expression (p < 0.05), indicating endoplasmic reticulum stress. Our data suggest concentration-dependent toxicological effects of SEOM-PM2.5 on NL-20 cells, including genotoxicity, genomic instability, and endoplasmic reticulum stress.
Assuntos
Poluentes Atmosféricos , Brônquios , Sobrevivência Celular , Células Epiteliais , Material Particulado , Hidrocarbonetos Policíclicos Aromáticos , Solventes , Humanos , Células Epiteliais/efeitos dos fármacos , Material Particulado/toxicidade , Linhagem Celular , Poluentes Atmosféricos/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Brônquios/citologia , Brônquios/efeitos dos fármacos , Solventes/toxicidade , Solventes/química , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , México , Espécies Reativas de Oxigênio/metabolismoRESUMO
Air pollution is a worldwide environmental problem with an impact on human health. Particulate matter of ten micrometers or less aerodynamic diameter (PM10) as well as its fine fraction (PM2.5) is related to multiple pulmonary diseases. The impact of air pollution in Mexico City, and importantly, particulate matter has been studied and considered as a risk factor for two decades ago. Previous studies have reported the composition of Mexico City particulate matter, as well as the biological effects induced by this material. However, material collected and used in previous studies is a limited resource, and sampling and particle recovery techniques have been improved. In this study, we describe the methods used in our laboratory for Mexico City airborne particulate matter PM10 and PM2.5 sampling, considering the years 2017, 2018 and 2019. We also analyzed the PM10 and PM2.5 samples obtained to determine their composition. Finally, we exposed lung cell line cultures to PM10 and PM2.5 to evaluate the biological effect of the material in terms of cell viability, cell death, inflammatory response, and cytogenetic alterations. Our results showed that PM10 composition includes inorganic, organic and biological compounds, while PM2.5 is a mixture of more enriched organic compounds. PM10 and PM2.5 treatment in lung cells does not significantly impact cell viability/cell death. However, PM10 and PM2.5 increase the secretion levels of IL-6. Moreover, PM10 as well as PM2.5 induce cytogenetic alterations, such as micronuclei, anaphase bridges, trinucleated cells and apoptotic cells in lung cells. Our results update the evidence of the composition and biological effects of Mexico City particulate matter and provide us a reliable basis for future approaches.
Assuntos
Poluentes Atmosféricos , Poluição do Ar , Humanos , Material Particulado/toxicidade , Material Particulado/análise , Poluentes Atmosféricos/toxicidade , Poluentes Atmosféricos/análise , México , Poluição do Ar/análise , Cidades , Tamanho da PartículaRESUMO
Global ground-level measurements of elements in ambient particulate matter (PM) can provide valuable information to understand the distribution of dust and trace elements, assess health impacts, and investigate emission sources. We use X-ray fluorescence spectroscopy to characterize the elemental composition of PM samples collected from 27 globally distributed sites in the Surface PARTiculate mAtter Network (SPARTAN) over 2019-2023. Consistent protocols are applied to collect all samples and analyze them at one central laboratory, which facilitates comparison across different sites. Multiple quality assurance measures are performed, including applying reference materials that resemble typical PM samples, acceptance testing, and routine quality control. Method detection limits and uncertainties are estimated. Concentrations of dust and trace element oxides (TEO) are determined from the elemental dataset. In addition to sites in arid regions, a moderately high mean dust concentration (6 µg/m3) in PM2.5 is also found in Dhaka (Bangladesh) along with a high average TEO level (6 µg/m3). High carcinogenic risk (>1 cancer case per 100000 adults) from airborne arsenic is observed in Dhaka (Bangladesh), Kanpur (India), and Hanoi (Vietnam). Industries of informal lead-acid battery and e-waste recycling as well as coal-fired brick kilns likely contribute to the elevated trace element concentrations found in Dhaka.
RESUMO
Insulin resistance (IR) and metabolic disorders are non-pulmonary adverse effects induced by fine particulate matter (PM2.5) exposure. The worldwide pandemic of high fructose sweeteners and fat rich modern diets, also contribute to IR development. We investigated some of the underlying effects of IR, altered biochemical insulin action and Insulin/AKT pathway biomarkers. Male Sprague Dawley rats were subchronically exposed to filtered air, PM2.5, a fructose rich diet (FRD), or PM2.5 + FRD. Exposure to PM2.5 or FRD alone did not induce metabolic changes. However, PM2.5 + FRD induced leptin release, systemic hyperinsulinemia, and Insulin/AKT dysregulation in insulin-sensitive tissues preceded by altered AT1R levels. Histological damage and increased HOMA-IR were also observed from PM2.5 + FRD co-exposure. Our results indicate that the concomitant exposure to a ubiquitous environmental pollutant, such as PM2.5, and a metabolic disease risk factor, a FRD, can contribute to the metabolic disorder pandemic occurring in highly polluted locations.
Assuntos
Resistência à Insulina , Ratos , Animais , Masculino , Ratos Sprague-Dawley , Frutose/toxicidade , Material Particulado/toxicidade , Proteínas Proto-Oncogênicas c-akt , Dieta , Insulina/metabolismoRESUMO
Outdoor air pollution is responsible for the exacerbation of respiratory diseases in humans. Particulate matter with an aerodynamic diameter ≤2.5 µm (PM2.5) is one of the main components of outdoor air pollution, and solvent extracted organic matter (SEOM) is adsorbed to the main PM2.5 core. Some of the biological effects of black carbon and polycyclic aromatic hydrocarbons, which are components of PM2.5, are known, but the response of respiratory cell lineages to SEOM exposure has not been described until now. The aim of this study was to obtain SEOM from PM2.5 and analyze the molecular and proteomic effects on human type II pneumocytes. PM2.5 was collected from Mexico City in the wildfire season and the SEOM was characterized to be exposed on human type II pneumocytes. The effects were compared with benzo [a] pyrene (B[a]P) and hydrogen peroxide (H2O2). The results showed that SEOM induced a decrease in surfactant and deregulation in the molecular protein and lipid pattern analyzed by reflection-Fourier transform infrared (ATR-FTIR) spectroscopy on human type II pneumocytes after 24 h. The molecular alterations induced by SEOM were not shared by those induced by B[a]P nor H2O2, which highlights specific SEOM effects. In addition, proteomic patterns by quantitative MS analysis revealed a downregulation of 171 proteins and upregulation of 134 proteins analyzed in the STRING database. The deregulation was associated with positive regulation of apoptotic clearance, removal of superoxide radicals, and positive regulation of heterotypic cell-cell adhesion processes, while ATP metabolism, nucleotide process, and cellular metabolism were also affected. Through this study, we conclude that SEOM extracted from PM2.5 exerts alterations in molecular patterns of protein and lipids, surfactant expression, and deregulation of metabolic pathways of type II pneumocytes after 24 h of exposure in absence of cytotoxicity, which warns about apparent SEOM silent effects.
Assuntos
Poluentes Atmosféricos , Poluição do Ar , Humanos , Poluentes Atmosféricos/toxicidade , Poluentes Atmosféricos/análise , Células Epiteliais Alveolares/química , Peróxido de Hidrogênio/análise , Proteômica , Monitoramento Ambiental/métodos , Poluição do Ar/análise , Material Particulado/toxicidade , Material Particulado/análise , Tensoativos/análiseRESUMO
We evaluated impacts of progressive technological updates to bus rapid transit (BRT) systems on in-cabin concentrations of particulate matter with an aerodynamic diameter ≤2.5 µm (PM2.5), and the various polyaromatic hydrocarbons (PAHs) to which commuters were exposed. PM2.5 samples were collected and real-time concentrations measured from October 2017 to March 2020 inside cabins of BRT buses equipped with Euro IV, V and VI diesel emission standards in the Mexico City Metropolitan Area (MCMA). For effective comparison, similar samplings and measurements were carried out on trains in the MCMA underground (MCU) system. Peak in-cabin PM2.5 concentrations decreased significantly (p < 0.05) by 35% from Euro IV to Euro V buses, and by 80% from Euro IV to Euro VI buses. PM2.5 concentrations inside Euro VI buses were significantly lower (p < 0.05) than in Euro IV and Euro V buses and in underground trains. The in-cabin excess (ICE) of PM2.5 relative to ambient concentrations was significantly (p < 0.05) higher for Euro IV than for Euro V buses during morning the traffic peak, and consistently higher than for Euro VI buses. Indeed, ICEs calculated for Euro VI buses were always lower than those for electricity-powered underground trains. The frequency of hotspots decreased from Euro IV to Euro VI buses due to the combined effect of low emissions and closed, air-conditioned cabins. Concentrations of total PAHs including carcinogenic species also decreased from Euro IV to Euro V buses and were below limits of detection aboard Euro VI buses. This work shows that in real-life conditions, advanced diesel technologies and cabin design significantly reduce commuters' exposure to PM2.5 and to toxic PAH compounds.
Assuntos
Poluentes Atmosféricos , Poluentes Atmosféricos/análise , Emissões de Veículos/análise , México , Material Particulado/análise , Veículos Automotores , Monitoramento AmbientalRESUMO
Pollution levels of organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) were investigated in the El Sol and the La Luna alpine lakes. The lakes are located in central Mexico, in the crater of the Nevado de Toluca volcano. The El Sol and the La Luna lakes are extremely relevant in Mexico and in the world because they are recognized as pristine regions and environmental reservoirs. Samples of atmospheric aerosol, sediment, plankton, and Tubifex tubifex (sludge worm) were collected at three different sample locations for three years (2017, 2018, and 2019) at three different times of year, meaning that the weather conditions at the time of sampling were different. Pollutants were analysed by gas chromatography-mass spectrometry with negative chemical ionisation (GC-MS/NCI). Endosulfan was the most frequent and abundant pollutant, showing the highest peaks of all. Atmospheric aerosol revealed Σ2 = 45 pg/m3, including α and ß, while sediment lakes displayed α, ß and endosulfan sulfate as Σ3 = 1963 pg/g, whereas plankton and Tubifex tubifex showed Σ2 = 576 pg/g and 540 pg/g for α and ß respectively. Results of endosulfan ratios (α/ß) and (α-ß/endosulfan sulfate) suggest that both fresh and old discharges continue to arrive at the lakes. This study shows for the first time the pollution levels of OCP and PCB in high mountain lakes in Mexico. These results that must be considered by policy makers to mitigate their use in the various productive activities of the region.
Assuntos
Poluentes Ambientais , Hidrocarbonetos Clorados , Praguicidas , Bifenilos Policlorados , Poluentes Químicos da Água , Endossulfano/análise , Monitoramento Ambiental/métodos , Poluentes Ambientais/análise , Hidrocarbonetos Clorados/análise , Lagos/análise , México , Praguicidas/análise , Bifenilos Policlorados/análise , Poluentes Químicos da Água/análiseRESUMO
Public concern has emerged about the effects of endocrine-disrupting compounds (EDCs) on neuropsychiatric disorders. Preclinical evidence suggests that exposure to EDCs is associated with the development of major depressive disorder (MDD) and could result in neural degeneration. The interaction of EDCs with hormonal receptors is the best-described mechanism of their biological activity. However, the dysregulation of the hypothalamic-pituitary-gonadal adrenal axis has been reported and linked to neurological disorders. At a worldwide level and in Mexico, the incidence of MDD has recently been increasing. Of note, in Mexico, there are no clinical associations on blood levels of EDCs and the incidence of the MDD. Methodology: Thus, we quantified for the first time the serum levels of parent compounds of two bisphenols and four phthalates in patients with MDD. The levels of di-ethyl-hexyl-phthalate (DEHP), butyl-benzyl-phthalate (BBP), di-n-butyl phthalate (DBP), and di-ethyl-phthalate (DEP), bisphenol A (BPA), and bisphenol S (BPS) in men and women with or without MDD were determined with a gas chromatograph-mass spectrometer. Results/conclusion: We found significant differences between concentrations of BBP between controls and patients with MDD. Interestingly, the serum levels of this compound have a dysmorphic behavior, being much higher in women (~500 ng/mL) than in men (≤10 ng/mL). We did not observe significant changes in the serum concentrations of the other phthalates or bisphenols tested, neither when comparing healthy and sick subjects nor when they were compared by gender. The results point out that BBP has a critical impact on the etiology of MDD disorder in Mexican patients, specifically in women.
Assuntos
Transtorno Depressivo Maior , Disruptores Endócrinos , Ácidos Ftálicos , Transtorno Depressivo Maior/epidemiologia , Dibutilftalato , Disruptores Endócrinos/toxicidade , Exposição Ambiental/estatística & dados numéricos , Poluição Ambiental/estatística & dados numéricos , Feminino , Humanos , Masculino , Ácidos Ftálicos/toxicidadeRESUMO
Exposure to fine particulate matter (PM2.5) induces airway inflammation and hyperreactivity that lead to asthma. The mechanisms involved are still under investigation. We investigated the effect of resveratrol (3,4',5-trihydroxystilbene) (RES) on airway hyperresponsiveness, inflammation and CYP1A1 protein expression (an aryl hydrocarbon receptor (AhR) target) induced by PM2.5 exposure in an allergic asthma experimental guinea pig model. The polyphenolic compound RES was used due to its antioxidant and anti-inflammatory properties and as an antagonist of the AhR; thus, providing mechanistic insights. Animals were sensitized with aluminum hydroxide and ovalbumin and exposed to filtered air or PM2.5. Exposure to PM2.5 was conducted using a whole-body chamber particle concentrator (5 h/day) for 15 days. Animals received saline solution or RES (10 mg/kg per day) orally for 21 days simultaneously to the OVA challenge or PM2.5 exposure. PM2.5 exposure (mean 433 ± 111 µg/m3 in the exposure chamber) in OVA challenged animals induced an asthma-like phenotype characterized by increased baseline lung resistance (Rrs) and central airway resistance (Rn) in response to acetylcholine (ACh) evaluated using a flexiVent system®. A parallel increase of pro-inflammatory cytokines (IL-6, IL-17, TNF-α and IFN-γ), inflammatory cells (eosinophils and neutrophils) in bronchoalveolar lavage fluid (BALF) and lung CYP1A1 increase also occurred. RES significantly inhibited airway hyperresponsiveness, inflammation, and CYP1A1 protein expression in the OVA-challenged PM2.5 exposed animals. In summary, with the use of RES we demonstrate that PM-induced airway hyperreactivity is modulated by the inflammatory response via the AhR pathway in an allergic asthma guinea pig model.
Assuntos
Asma/induzido quimicamente , Fatores de Transcrição Hélice-Alça-Hélice Básicos/agonistas , Pulmão/efeitos dos fármacos , Material Particulado/toxicidade , Pneumonia/induzido quimicamente , Receptores de Hidrocarboneto Arílico/agonistas , Hidróxido de Alumínio , Animais , Antiasmáticos/farmacologia , Anti-Inflamatórios/farmacologia , Asma/imunologia , Asma/metabolismo , Asma/prevenção & controle , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Citocromo P-450 CYP1A1/metabolismo , Citocinas/metabolismo , Modelos Animais de Doenças , Cobaias , Mediadores da Inflamação/metabolismo , Pulmão/imunologia , Pulmão/metabolismo , Ovalbumina , Tamanho da Partícula , Pneumonia/imunologia , Pneumonia/metabolismo , Pneumonia/prevenção & controle , Receptores de Hidrocarboneto Arílico/metabolismo , Resveratrol/farmacologia , Transdução de SinaisRESUMO
Phthalates and bisphenols are ubiquitous environmental pollutants with the ability to perturb different systems. Specifically, they can alter the endocrine system, and this is why they are also known as endocrine-disrupting compounds (EDCs). Interestingly, they are related to the development and progression of breast cancer (BC), but the threshold concentrations at which they trigger that are not well established. OBJECTIVES: The aim of this study was to compare the concentration measures of parent EDCs in three groups of women (without BC, with BC, and BC survivors) from two urban populations in Mexico, to establish a possible association between EDCs and this disease. We consider the measure of the parent compounds would reflect the individual's exposure. METHODS: The levels of di-ethyl-hexyl-phthalate (DEHP), butyl-benzyl-phthalate (BBP), di-n-butyl phthalate (DBP) and di-ethyl-phthalate (DEP), bisphenol A (BPA) and bisphenol S (BPS) were determined by gas chromatograph-mass spectrometry in 102 subjects, including 37 women without any pathological disease, 46 patients with BC and 19 women survivals of BC of Mexico and Toluca City. RESULTS: All phthalates were detected in 100% of women, two of them were significantly higher in patients with different BC subtypes in Mexico City. Differential increases were observed mainly in the serum concentration of phthalates in women with BC compared to women without disease between Mexico and Toluca City. In addition, when performing an analysis of the concentrations of phthalates by molecular type of BC, DEP and BBP were found mainly in aggressive and poorly differentiated types of BC. It should be noted that female BC survivors treated with anti-hormonal therapy showed lower levels of BBP than patients with BC. BPA and BPS were found in most samples from Mexico City. However, BPS was undetectable in women from Toluca City. DISCUSSION: The results of our study support the hypothesis of a positive association between exposure to phthalates and BC incidence.
Assuntos
Neoplasias da Mama , Disruptores Endócrinos , Ácidos Ftálicos , Compostos Benzidrílicos/análise , Neoplasias da Mama/epidemiologia , Exposição Ambiental/análise , Feminino , Humanos , Fenóis , Ácidos Ftálicos/análise , Plastificantes/análise , SobreviventesRESUMO
The black carbon is a pollutant species primarily emitted from the combustion of fossil fuels (diesel). Their concentrations associated to PM2.5 were monitoring at two sites in the city of Guadalajara. From January to May (except April), downtown site shown 2.7, 2.6, 4.0 and 2.3 times higher monthly concentrations. The dry season two showed higher concentrations respect to at least one of the others seasons (p < 0.0001) at each site, probably due to atmospheric conditions less favorable for the dispersal of pollutants. During the 24 h period were observed at the year two peaks of concentrations: the highest morning peak and lower night peak, both probably related to anthropogenic activity.
Assuntos
Poluentes Atmosféricos/análise , Monitoramento Ambiental/métodos , Material Particulado/análise , Fuligem/análise , Emissões de Veículos/análise , Cidades , México , Tamanho da Partícula , Estações do Ano , Fatores de TempoRESUMO
Mexico City has been classified as one megacity, its altitude, thermal inversions, and high seasonal radiation are factors that prevent dispersion of pollutants, which effects are detrimental to health. Therefore, it is important to have an organism that allows evaluate the damage caused by such exposure, as is the case of mosses that obtain nutrients from the atmosphere; this property makes them excellent biomonitors to evaluate genotoxic damage caused by exposure to pollutants, in addition to its large accumulation capacity. For these reasons and to relate the effects of atmospheric pollution with a biological response, we propose to use the moss Hypnum amabile as a bioaccumulator of atmospheric pollutants and biomonitor of the genotoxic effect that the air pollution can induce it through the comet assay. Mosses were placed in five localities of Mexico City and the metropolitan area on the first days of each month of the dry (cold and warm) and rainy seasons, with a 30-day exposure, after which they were changed for a new sample (for 8 months). Each month, the moss exposed was collected and nuclei were isolated to perform comet assay. To demonstrate heavy metal bioaccumulation capacity, samples were observed in a transmission electron microscope and qualitative microanalysis by scanning electron microscopy was carried out parallel. The chemical analysis detected 14 heavy metals by mass spectrometry method with inductively coupled plasma source. Additionally, 22 polycyclic aromatic hydrocarbons were also determined by gas chromatography-mass spectrometry. Analysis of variance and Kruskal-Wallis test were performed to compare DNA damage of each station against control, which was maintained in the laboratory in a chamber with filtered air. This is the first study on the genotoxicity of mosses exposed to the atmosphere of Mexico City and metropolitan area that in addition to proving their accumulation capacity shows their ability to respond to atmospheric pollutants.
Assuntos
Poluentes Atmosféricos , Briófitas , Poluentes Ambientais , Metais Pesados , Poluentes Atmosféricos/análise , Cidades , Dano ao DNA , Monitoramento Ambiental , Cromatografia Gasosa-Espectrometria de Massas , Metais Pesados/análise , MéxicoRESUMO
The exposure to environmental pollutants, such as fine and ultrafine particles (FP and UFP), has been associated with increased risk for Parkinson's disease, depression and schizophrenia, disorders related to altered dopaminergic transmission. The striatum, a neuronal nucleus with extensive dopaminergic afferents, is a target site for particle toxicity, which results in oxidative stress, inflammation, astrocyte activation and modifications in dopamine content and D2 receptor (D2R) density. In this study we assessed the in vitro effect of the exposure to FP and UFP on dopaminergic transmission, by evaluating [3H]-dopamine uptake and release by rat striatal isolated nerve terminals (synaptosomes), as well as modifications in the affinity and signaling of native and cloned D2Rs. FP and UFP collected from the air of Mexico City inhibited [3H]-dopamine uptake and increased depolarization-evoked [3H]-dopamine release in striatal synaptosomes. FP and UFP also enhanced D2R affinity for dopamine in membranes from either rat striatum or CHO-K1 cells transfected with the long isoform of the human D2R (hD2LR)2LR). In CHO-K1-hD2L In CHO-K1-hD2LR cells or striatal slices, FP and UFP increased the potency of dopamine or the D2R agonist quinpirole, respectively, to inhibit forskolin-induced cAMP formation. The effects were concentration-dependent, with UFP being more potent than FP. These results indicate that FP and UFP directly affect dopaminergic transmission.
Assuntos
Poluentes Atmosféricos/toxicidade , Corpo Estriado/efeitos dos fármacos , Dopamina/metabolismo , Material Particulado/toxicidade , Animais , Células CHO , Corpo Estriado/metabolismo , Cricetulus , Técnicas In Vitro , Masculino , México , Ratos Wistar , Receptores de Dopamina D2/metabolismo , Transdução de Sinais/efeitos dos fármacos , Sinaptossomos/efeitos dos fármacos , Sinaptossomos/metabolismoRESUMO
A pilot study was initiated in 2018 under the Global Atmospheric Passive Sampling (GAPS) Network named GAPS-Megacities. This study included 20 megacities/major cities across the globe with the goal of better understanding and comparing ambient air levels of persistent organic pollutants and other chemicals of emerging concern, to which humans residing in large cities are exposed. The first results from the initial period of sampling are reported for 19 cities for several classes of flame retardants (FRs) including organophosphate esters (OPEs), polybrominated diphenyl ethers (PBDEs), and halogenated flame retardants (HFRs) including new flame retardants (NFRs), tetrabromobisphenol A (TBBPA) and hexabromocyclododecane (HBCDD). The two cities, New York (USA) and London (UK) stood out with â¼3.5 to 30 times higher total FR concentrations as compared to other major cities, with total concentrations of OPEs of 15,100 and 14,100 pg/m3, respectively. Atmospheric concentrations of OPEs significantly dominated the FR profile at all sites, with total concentrations in air that were 2-5 orders of magnitude higher compared to other targeted chemical classes. A moderately strong and significant correlation (r = 0.625, p < 0.001) was observed for Gross Domestic Product index of the cities with total OPEs levels. Although large differences in FR levels were observed between some cities, when averaged across the five United Nations regions, the FR classes were more evenly distributed and varied by less than a factor of five. Results for Toronto, which is a 'reference city' for this study, agreed well with a more in-depth investigation of the level of FRs over different seasons and across eight sites representing different urban source sectors (e.g. traffic, industrial, residential and background). Future sampling periods under this project will investigate trace metals and other contaminant classes, linkages to toxicology, non-targeted analysis, and eventually temporal trends. The study provides a unique urban platform for evaluating global exposome.