Organomineral fertilizer application enhances Perilla frutescens nutritional quality and rhizosphere microbial community stability in karst mountain soils.

Introduction: Applications of organomineral fertilizer (OMF) are important measures for developing organic agriculture in karst mountain areas. However, the influence of OMF on the structure and function of soil microbial diversity and their relationship with crop yield and quality are still unclear. Methods: Based on soil science, crop science, and high-throughput sequencing methods, we investigated the changes of rhizosphere soil microbial communities of Perilla frutescens under different fertilization measures. Then, the relationship between P. frutescens yield and quality with soil quality was analyzed. Results: The results showed that the addition of OMF increased the amount of total carbon and total potassium in soil. OF, especially OMF, improved P. frutescens yield and quality (e.g., panicle number per plant, main panicle length, and unsaturated fatty acid contents). Both OF and OMF treatments significantly increased the enrichment of beneficial microorganism (e.g., Bacillus, Actinomadura, Candidatus_Solibacter, Iamia, Pseudallescheria, and Cladorrhinum). The symbiotic network analysis demonstrated that OMF strengthened the connection among the soil microbial communities, and the community composition became more stable. Redundancy analysis and structural equation modeling showed that the soil pH, available phosphorus, and available potassium were significantly correlated with soil microbial community diversity and P. frutescens yield and quality. Discussion: Our study confirmed that OMF could replace CF or common OF to improve soil fertility, crop yield and quality in karst mountain soils.

Effect and mechanism of the improvement of coastal silt soil by application of organic fertilizer and gravel combined with Sesbania cannabina cultivation.

Jiangsu Province of China has a large area of coastal silt soil (CSS) with poor permeability, high salinity, and poor nutrients, which brings great difficulties to the development and utilization of coastal zones, so that needs to be improved as a matter of urgency. In this study, river-sand, serpentine, and organic fertilizer were used as additives in CSS, and Sesbania cannabina, a salt-tolerant cash crop, was planted in these differently treated soils. Through high-throughput sequencing, analysis of soil physico-chemical properties, and detection of plant growth status, the rhizosphere bacterial diversity of S. cannabina growing in CSS under different treatments and their environmental impact factors were studied, while exploring the effect and mechanism of organic fertilizer combined with gravel as a CSS modifier. The results implied that single application of organic fertilizer could significantly increase the fertility levels of total nitrogen (TN), total organic carbon (TOC) and Avail. P in CSS; then, the application of organic fertilizer with river-sand significantly reduced salt content and alkalinity of soil; meanwhile, in the treatment of single application of organic fertilizer and application of organic fertilizer combined with river-sand, the rhizosphere of S. cannabina enriched the bacterial communities of organic matter degradation and utilization to varying degrees. The soil moisture content and indicators related to saline-alkali soil (including total salt, electrical conductivity (EC), exchangeable sodium percentage (ESP), Avail. Na and Avail. K, etc.) were further reduced significantly by the application of organic fertilizer combined with river-sand and serpentine. The method has greatly improved the growth conditions of S. cannabina and promoted the positive development of its rhizosphere bacterial community. Among them, in the treatment of organic fertilizer combined with river-sand and serpentine, a variety of plant growth-promoting rhizobacteria (PGPR, such as Sphingomonas, Ensifer, and Rhodobacter) and nitrogen-cycle-related bacteria (such as nitrate-reduction-related bacteria, nitrogen-fixing bacteria like Ensifer, and purple non-sulfur photosynthetic bacteria like Rhodobacter) were enriched in the rhizosphere of S. cannabina; moreover, the mutual association and robustness of bacterial co-occurrence networks have been significantly enhanced. The results provide a theoretical basis and reference model for the improvement of coastal saline-alkali silt soil.

Soil microbial community structure and environmental effects of serpentine weathering under different vegetative covers in the serpentine mining area of Donghai County, China.

The use of serpentine biological weathering to capture atmospheric CO2 has attracted much attention. In the long-term mining activities in a serpentine mining area, a large amount of serpentine powder diffused into the surrounding forest and farmland soil. The study of the serpentine weathering in soils of different vegetative covers and the composition characteristics of soil carbonate has important implications for understanding the serpentine weathering and carbon sequestration under natural conditions. The microbial diversity on exposed rock serpentine surfaces and soil under different vegetative covers in the serpentine mining area in Donghai County, China was investigated by high-throughput sequencing technology, and the characteristics of serpentine weathering and soil carbonate in related area were also explored by XRF, XRD, SEM-EDS, and chemical analysis methods. The results showed that the richness and uniformity of the bacteria species community increased significantly with the increasing complexity of plant groups covering the rock surface, but the species richness and uniformity of fungi showed an overall declining trend. Furthermore, high­magnesium calcite (HMC) is ubiquitous on the exposed rock surface and the soil under different vegetative covers in this area. Based on these results, combined with the verification test results of HMC fixed heavy metal ions, the model of serpentine weathering in serpentine mining soil to synthesize carbonate and fix heavy metal ions was developed. That is, with the increase in the degree of rock weathering and the colonization of plants, the soil and plants seem to shape jointly a relatively stable microbial community structure adapted to the environment of the serpentine mining area, which promotes the serpentine weathering coupled with the formation of HMC and immobilization of metal ions in the serpentine soil. This study provides a theoretical basis for the serpentine bio-weathering in the mine area to capture atmospheric CO2.

Characterisation of products from fungally modified wollastonite and the simulation experiment of Pb2+ fixation.

Using cheap raw materials and simple biological modification methods to obtain modified materials to remediate heavy metals has potential application prospects. Here, Aspergillus niger was used to modify wollastonite and further explore its fixation capacity and mechanism. The results showed that the growth and metabolism of A. niger promoted the weathering of wollastonite and the synthesis of whewellite, forming a mineral composite through fungal modification (MCF) with excellent fixation properties of heavy metal Pb2+. The maximum adsorption capacity of MCF for Pb2+ (434.78 mg/g) is significantly greater than that of original wollastonite (11.86 mg/g) with a desired lower desorption rate. Additionally, its removal rate of Pb2+ is higher than 75% under varying acidic conditions (1 ≤ pH ≤ 5.5). The results of XRD, FTIR, BET specific surface area, and SEM-EDS showed that MCF has a special organic-inorganic composite structure, which imparts a larger contact area for ions, and efficient removal of Pb2+ through combined physico-chemical adsorption. Batch adsorption results also indicated that the adsorption process by MCF was mainly a spontaneous endothermic reaction in the monolayer. This study provides a new perspective for the bio-modification of wollastonite and its application in the remediation of heavy metal pollution.