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Characterizing cellular diversity at different levels of biological organization and across data modalities is a prerequisite to understanding the function of cell types in the brain. Classification of neurons is also essential to manipulate cell types in controlled ways and to understand their variation and vulnerability in brain disorders. The BRAIN Initiative Cell Census Network (BICCN) is an integrated network of data-generating centers, data archives, and data standards developers, with the goal of systematic multimodal brain cell type profiling and characterization. Emphasis of the BICCN is on the whole mouse brain with demonstration of prototype feasibility for human and nonhuman primate (NHP) brains. Here, we provide a guide to the cellular and spatial approaches employed by the BICCN, and to accessing and using these data and extensive resources, including the BRAIN Cell Data Center (BCDC), which serves to manage and integrate data across the ecosystem. We illustrate the power of the BICCN data ecosystem through vignettes highlighting several BICCN analysis and visualization tools. Finally, we present emerging standards that have been developed or adopted toward Findable, Accessible, Interoperable, and Reusable (FAIR) neuroscience. The combined BICCN ecosystem provides a comprehensive resource for the exploration and analysis of cell types in the brain.
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Encéfalo , Neurociências , Animais , Humanos , Camundongos , Ecossistema , NeurôniosRESUMO
Dendrite shape impacts functional connectivity and is mediated by organization and dynamics of cytoskeletal fibers. Identifying the molecular factors that regulate dendritic cytoskeletal architecture is therefore important in understanding the mechanistic links between cytoskeletal organization and neuronal function. We identified Formin 3 (Form3) as an essential regulator of cytoskeletal architecture in nociceptive sensory neurons in Drosophila larvae. Time course analyses reveal that Form3 is cell-autonomously required to promote dendritic arbor complexity. We show that form3 is required for the maintenance of a population of stable dendritic microtubules (MTs), and mutants exhibit defects in the localization of dendritic mitochondria, satellite Golgi, and the TRPA channel Painless. Form3 directly interacts with MTs via FH1-FH2 domains. Mutations in human inverted formin 2 (INF2; ortholog of form3) have been causally linked to Charcot-Marie-Tooth (CMT) disease. CMT sensory neuropathies lead to impaired peripheral sensitivity. Defects in form3 function in nociceptive neurons result in severe impairment of noxious heat-evoked behaviors. Expression of the INF2 FH1-FH2 domains partially recovers form3 defects in MTs and nocifensive behavior, suggesting conserved functions, thereby providing putative mechanistic insights into potential etiologies of CMT sensory neuropathies.
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Dendritos/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Forminas/metabolismo , Microtúbulos/metabolismo , Plasticidade Neuronal/genética , Nociceptividade , Actinas/metabolismo , Animais , Animais Geneticamente Modificados , Comportamento Animal , Citoesqueleto/metabolismo , Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Drosophila melanogaster/crescimento & desenvolvimento , Forminas/genética , Humanos , Mutação , Nociceptores/metabolismo , TransgenesRESUMO
SUMMARY: Neural morphology, the branching geometry of brain cells, is an essential cellular substrate of nervous system function and pathology. Despite the accelerating production of digital reconstructions of neural morphology, the public accessibility of data remains a core issue in neuroscience. Deficiencies in the availability of existing data create redundancy of research efforts and limit synergy. We carried out a comprehensive bibliometric analysis of neural morphology publications to quantify the impact of data sharing in the neuroscience community. Our findings demonstrate that sharing digital reconstructions of neural morphology via NeuroMorpho.Org leads to a significant increase of citations to the original article, thus directly benefiting authors. The rate of data reusage remains constant for at least 16 years after sharing (the whole period analyzed), altogether nearly doubling the peer-reviewed discoveries in the field. Furthermore, the recent availability of larger and more numerous datasets fostered integrative applications, which accrue on average twice the citations of re-analyses of individual datasets. We also released an open-source citation tracking web-service allowing researchers to monitor reusage of their datasets in independent peer-reviewed reports. These results and tools can facilitate the recognition of shared data reuse for merit evaluations and funding decisions. AVAILABILITY AND IMPLEMENTATION: The application is available at: http://cng-nmo-dev3.orc.gmu.edu:8181/. The source code at https://github.com/HerveEmissah/nmo-authors-app and https://github.com/HerveEmissah/nmo-bibliometric-analysis.
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Neurociências , Neurociências/métodos , Disseminação de Informação , Neurônios , Software , EncéfaloRESUMO
Understanding brain operation demands linking basic behavioral traits to cell-type specific dynamics of different brain-wide subcircuits. This requires a system to classify the basic operational modes of neurons and circuits. Single-cell phenotyping of firing behavior during ongoing oscillations in vivo has provided a large body of evidence on entorhinal-hippocampal function, but data are dispersed and diverse. Here, we mined literature to search for information regarding the phase-timing dynamics of over 100 hippocampal/entorhinal neuron types defined in Hippocampome.org. We identified missing and unresolved pieces of knowledge (e.g., the preferred theta phase for a specific neuron type) and complemented the dataset with our own new data. By confronting the effect of brain state and recording methods, we highlight the equivalences and differences across conditions and offer a number of novel observations. We show how a heuristic approach based on oscillatory features of morphologically identified neurons can aid in classifying extracellular recordings of single cells and discuss future opportunities and challenges towards integrating single-cell phenotypes with circuit function.
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Hipocampo/anatomia & histologia , Hipocampo/metabolismo , Hipocampo/fisiologia , Potenciais de Ação/fisiologia , Animais , Córtex Entorrinal/fisiologia , Camundongos , Neurônios/fisiologia , Fenótipo , RatosRESUMO
Computational simulations with data-driven physiological detail can foster a deeper understanding of the neural mechanisms involved in cognition. Here, we utilize the wealth of cellular properties from Hippocampome.org to study neural mechanisms of spatial coding with a spiking continuous attractor network model of medial entorhinal cortex circuit activity. The primary goal is to investigate if adding such realistic constraints could produce firing patterns similar to those measured in real neurons. Biological characteristics included in the work are excitability, connectivity, and synaptic signaling of neuron types defined primarily by their axonal and dendritic morphologies. We investigate the spiking dynamics in specific neuron types and the synaptic activities between groups of neurons. Modeling the rodent hippocampal formation keeps the simulations to a computationally reasonable scale while also anchoring the parameters and results to experimental measurements. Our model generates grid cell activity that well matches the spacing, size, and firing rates of grid fields recorded in live behaving animals from both published datasets and new experiments performed for this study. Our simulations also recreate different scales of those properties, e.g., small and large, as found along the dorsoventral axis of the medial entorhinal cortex. Computational exploration of neuronal and synaptic model parameters reveals that a broad range of neural properties produce grid fields in the simulation. These results demonstrate that the continuous attractor network model of grid cells is compatible with a spiking neural network implementation sourcing data-driven biophysical and anatomical parameters from Hippocampome.org. The software (version 1.0) is released as open source to enable broad community reuse and encourage novel applications.
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Potenciais de Ação , Córtex Entorrinal , Células de Grade , Modelos Neurológicos , Sinapses , Animais , Células de Grade/fisiologia , Sinapses/fisiologia , Córtex Entorrinal/fisiologia , Córtex Entorrinal/citologia , Potenciais de Ação/fisiologia , Simulação por Computador , Neurônios/fisiologia , Neurônios/citologia , Hipocampo/fisiologia , Hipocampo/citologia , Rede Nervosa/fisiologia , Rede Nervosa/citologia , Redes Neurais de ComputaçãoRESUMO
Neurons and glia are the two main cell classes in the nervous systems of most animals. Although functionally distinct, neurons and glia are both characterized by multiple branching arbors stemming from the cell bodies. Glial processes are generally known to form smaller trees than neuronal dendrites. However, the full extent of morphological differences between neurons and glia in multiple species and brain regions has not yet been characterized, nor is it known whether these cells can be reliably distinguished based on geometric features alone. Here, we show that multiple supervised learning algorithms deployed on a large database of morphological reconstructions can systematically classify neuronal and glial arbors with nearly perfect accuracy and precision. Moreover, we report multiple morphometric properties, both size related and size independent, that differ substantially between these cell types. In particular, we newly identify an individual morphometric measurement, Average Branch Euclidean Length that can robustly separate neurons from glia across multiple animal models, a broad diversity of experimental conditions, and anatomical areas, with the notable exception of the cerebellum. We discuss the practical utility and physiological interpretation of this discovery.
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Neuroglia , Neurônios , Animais , Neurônios/fisiologia , Encéfalo , Aprendizado de Máquina , BiomarcadoresRESUMO
MOTIVATION: Large-scale neuronal morphologies are essential to neuronal typing, connectivity characterization and brain modeling. It is widely accepted that automation is critical to the production of neuronal morphology. Despite previous survey papers about neuron tracing from light microscopy data in the last decade, thanks to the rapid development of the field, there is a need to update recent progress in a review focusing on new methods and remarkable applications. RESULTS: This review outlines neuron tracing in various scenarios with the goal to help the community understand and navigate tools and resources. We describe the status, examples and accessibility of automatic neuron tracing. We survey recent advances of the increasingly popular deep-learning enhanced methods. We highlight the semi-automatic methods for single neuron tracing of mammalian whole brains as well as the resulting datasets, each containing thousands of full neuron morphologies. Finally, we exemplify the commonly used datasets and metrics for neuron tracing bench testing.
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Aprendizado Profundo , Microscopia , Animais , Microscopia/métodos , Imageamento Tridimensional/métodos , Algoritmos , Neurônios , Automação , MamíferosRESUMO
Dendritic morphology underlies the source and processing of neuronal signal inputs. Morphology can be broadly described by two types of geometric characteristics. The first is dendrogram topology, defined by the length and frequency of the arbor branches; the second is spatial embedding, mainly determined by branch angles and straightness. We have previously demonstrated that microtubules and actin filaments are associated with arbor elongation and branching, fully constraining dendrogram topology. Here, we relate the local distribution of these two primary cytoskeletal components with dendritic spatial embedding. We first reconstruct and analyze 167 sensory neurons from the Drosophila larva encompassing multiple cell classes and genotypes. We observe that branches with a higher microtubule concentration tend to deviate less from the direction of their parent branch across all neuron types. Higher microtubule branches are also overall straighter. F-actin displays a similar effect on angular deviation and branch straightness, but not as consistently across all neuron types as microtubule. These observations raise the question as to whether the associations between cytoskeletal distributions and arbor geometry are sufficient constraints to reproduce type-specific dendritic architecture. Therefore, we create a computational model of dendritic morphology purely constrained by the cytoskeletal composition measured from real neurons. The model quantitatively captures both spatial embedding and dendrogram topology across all tested neuron groups. These results suggest a common developmental mechanism regulating diverse morphologies, where the local cytoskeletal distribution can fully specify the overall emergent geometry of dendritic arbors.
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Proteínas de Drosophila , Drosophila , Animais , Drosophila/metabolismo , Actinas/metabolismo , Proteínas de Drosophila/metabolismo , Dendritos/metabolismo , Microtúbulos/metabolismo , Células Receptoras Sensoriais/metabolismo , Citoesqueleto de Actina/metabolismoRESUMO
Dendrites are the primary points of sensory or synaptic input to a neuron and play an essential role in synaptic integration and neural function. Despite the functional importance of dendrites, relatively less is known about the underlying mechanisms regulating cell type-specific dendritic patterning. Herein, we have dissected the functional roles of a previously uncharacterized gene, CG3995, in cell type-specific dendritic development in Drosophila melanogaster. CG3995, which we have named bedwarfed (bdwf), encodes a zinc-finger BED-type protein that is required for proportional growth and branching of dendritic arbors. It also exhibits nucleocytoplasmic expression and functions in both transcriptional and translational cellular pathways. At the transcriptional level, we demonstrate a reciprocal regulatory relationship between Bdwf and the homeodomain transcription factor (TF) Cut. We show that Cut positively regulates Bdwf expression and that Bdwf acts as a downstream effector of Cut-mediated dendritic development, whereas overexpression of Bdwf negatively regulates Cut expression in multidendritic sensory neurons. Proteomic analyses revealed that Bdwf interacts with ribosomal proteins and disruption of these proteins resulted in phenotypically similar dendritic hypotrophy defects as observed in bdwf mutant neurons. We further demonstrate that Bdwf and its ribosomal protein interactors are required for normal microtubule and F-actin cytoskeletal architecture. Finally, our findings reveal that Bdwf is required to promote protein translation and ribosome trafficking along the dendritic arbor. These findings shed light on the complex, combinatorial, and multi-functional roles of transcription factors (TFs) in directing the diversification of cell type-specific dendritic development.
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Proteínas de Drosophila , Fatores de Transcrição , Animais , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Drosophila/metabolismo , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Proteínas de Drosophila/metabolismo , Proteômica , Zinco/metabolismo , Dendritos/metabolismo , Proteínas de Homeodomínio/genética , Células Receptoras Sensoriais/metabolismoRESUMO
A quantitative description of the hippocampal formation synaptic architecture is essential for understanding the neural mechanisms of episodic memory. Yet the existing knowledge of connectivity statistics between different neuron types in the rodent hippocampus only captures a mere 5% of this circuitry. We present a systematic pipeline to produce first-approximation estimates for most of the missing information. Leveraging the www.Hippocampome.org knowledge base, we derive local connection parameters between distinct pairs of morphologically identified neuron types based on their axonal-dendritic overlap within every layer and subregion of the hippocampal formation. Specifically, we adapt modern image analysis technology to determine the parcel-specific neurite lengths of every neuron type from representative morphologic reconstructions obtained from either sex. We then compute the average number of synapses per neuron pair using relevant anatomic volumes from the mouse brain atlas and ultrastructurally established interaction distances. Hence, we estimate connection probabilities and number of contacts for >1900 neuron type pairs, increasing the available quantitative assessments more than 11-fold. Connectivity statistics thus remain unknown for only a minority of potential synapses in the hippocampal formation, including those involving long-range (23%) or perisomatic (6%) connections and neuron types without morphologic tracings (7%). The described approach also yields approximate measurements of synaptic distances from the soma along the dendritic and axonal paths, which may affect signal attenuation and delay. Overall, this dataset fills a substantial gap in quantitatively describing hippocampal circuits and provides useful model specifications for biologically realistic neural network simulations, until further direct experimental data become available.SIGNIFICANCE STATEMENT The hippocampal formation is a crucial functional substrate for episodic memory and spatial representation. Characterizing the complex neuron type circuit of this brain region is thus important to understand the cellular mechanisms of learning and navigation. Here we present the first numerical estimates of connection probabilities, numbers of contacts per connected pair, and synaptic distances from the soma along the axonal and dendritic paths, for more than 1900 distinct neuron type pairs throughout the dentate gyrus, CA3, CA2, CA1, subiculum, and entorhinal cortex. This comprehensive dataset, publicly released online at www.Hippocampome.org, constitutes an unprecedented quantification of the majority of the local synaptic circuit for a prominent mammalian neural system and provides an essential foundation for data-driven, anatomically realistic neural network models.
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Axônios/ultraestrutura , Conectoma/métodos , Dendritos/ultraestrutura , Hipocampo/ultraestrutura , Sinapses/ultraestrutura , Animais , Conjuntos de Dados como Assunto , Feminino , Processamento de Imagem Assistida por Computador/métodos , Masculino , Camundongos , RatosRESUMO
High digital connectivity and a focus on reproducibility are contributing to an open science revolution in neuroscience. Repositories and platforms have emerged across the whole spectrum of subdisciplines, paving the way for a paradigm shift in the way we share, analyze, and reuse vast amounts of data collected across many laboratories. Here, we describe how open access web-based tools are changing the landscape and culture of neuroscience, highlighting six free resources that span subdisciplines from behavior to whole-brain mapping, circuits, neurons, and gene variants.
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Acesso à Informação , Encéfalo/fisiologia , Internet/tendências , Rede Nervosa/fisiologia , Neurônios/fisiologia , Animais , Encéfalo/citologia , Conjuntos de Dados como Assunto/tendências , Redes Reguladoras de Genes/fisiologia , Humanos , Rede Nervosa/citologiaRESUMO
Quantifying the population sizes of distinct neuron types in different anatomical regions is an essential step towards establishing a brain cell census. Although estimates exist for the total neuronal populations in different species, the number and definition of each specific neuron type are still intensively investigated. Hippocampome.org is an open-source knowledge base with morphological, physiological and molecular information for 122 neuron types in the rodent hippocampal formation. While such framework identifies all known neuron types in this system, their relative abundances remain largely unknown. This work quantitatively estimates the counts of all Hippocampome.org neuron types by literature mining and numerical optimization. We report the number of neurons in each type identified by main neurotransmitter (glutamate or GABA) and axonal-dendritic patterns throughout 26 subregions and layers of the dentate gyrus, Ammon's horn, subiculum and entorhinal cortex. We produce by sensitivity analysis reliable numerical ranges for each type and summarize the amounts across broad neuronal families defined by biomarkers expression and firing dynamics. Study of density distributions indicates that the number of dendritic-targeting interneurons, but not of other neuronal classes, is independent of anatomical volumes. All extracted values, experimental evidence and related software code are released on Hippocampome.org.
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Hipocampo , Roedores , Animais , Mineração de Dados , Córtex Entorrinal/metabolismo , Hipocampo/fisiologia , Humanos , Neurônios/fisiologiaRESUMO
Computational modeling has contributed to hippocampal research in a wide variety of ways and through a large diversity of approaches, reflecting the many advanced cognitive roles of this brain region. The intensively studied neuron type circuitry of the hippocampus is a particularly conducive substrate for spiking neural models. Here we present an online knowledge base of spiking neural network simulations of hippocampal functions. First, we overview theories involving the hippocampal formation in subjects such as spatial representation, learning, and memory. Then we describe an original literature mining process to organize published reports in various key aspects, including: (i) subject area (e.g., navigation, pattern completion, epilepsy); (ii) level of modeling detail (Hodgkin-Huxley, integrate-and-fire, etc.); and (iii) theoretical framework (attractor dynamics, oscillatory interference, self-organizing maps, and others). Moreover, every peer-reviewed publication is also annotated to indicate the specific neuron types represented in the network simulation, establishing a direct link with the Hippocampome.org portal. The web interface of the knowledge base enables dynamic content browsing and advanced searches, and consistently presents evidence supporting every annotation. Moreover, users are given access to several types of statistical reports about the collection, a selection of which is summarized in this paper. This open access resource thus provides an interactive platform to survey spiking neural network models of hippocampal functions, compare available computational methods, and foster ideas for suitable new directions of research.
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The cellular and synaptic architecture of the rodent hippocampus has been described in thousands of peer-reviewed publications. However, no human- or machine-readable public catalog of synaptic electrophysiology data exists for this or any other neural system. Harnessing state-of-the-art information technology, we have developed a cloud-based toolset for identifying empirical evidence from the scientific literature pertaining to synaptic electrophysiology, for extracting the experimental data of interest, and for linking each entry to relevant text or figure excerpts. Mining more than 1,200 published journal articles, we have identified eight different signal modalities quantified by 90 different methods to measure synaptic amplitude, kinetics, and plasticity in hippocampal neurons. We have designed a data structure that both reflects the differences and maintains the existing relations among experimental modalities. Moreover, we mapped every annotated experiment to identified potential connections, that is, specific pairs of presynaptic and postsynaptic neuron types. To this aim, we leveraged Hippocampome.org, an open-access knowledge base of morphologically, electrophysiologically, and molecularly characterized neuron types in the rodent hippocampal formation. Specifically, we have implemented a computational pipeline to systematically translate neuron type properties into formal queries in order to find all compatible potential connections. With this system, we have collected nearly 40,000 synaptic data entities covering 88% of the 3,120 potential connections in Hippocampome.org. Correcting membrane potentials with respect to liquid junction potentials significantly reduced the difference between theoretical and experimental reversal potentials, thereby enabling the accurate conversion of all synaptic amplitudes to conductance. This data set allows for large-scale hypothesis testing of the general rules governing synaptic signals. To illustrate these applications, we confirmed several expected correlations between synaptic measurements and their covariates while suggesting previously unreported ones. We release all data open-source at Hippocampome.org in order to further research across disciplines.
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Mineração de Dados/métodos , Fenômenos Eletrofisiológicos/fisiologia , Hipocampo/fisiologia , Bases de Conhecimento , Plasticidade Neuronal/fisiologia , Sinapses/fisiologia , Fatores Etários , Animais , Hipocampo/citologia , Masculino , RoedoresRESUMO
Gene and protein expressions are key determinants of cellular function. Neurons are the building blocks of brain circuits, yet the relationship between their molecular identity and the spatial distribution of their dendritic inputs and axonal outputs remains incompletely understood. The open-source knowledge base Hippocampome.org amasses such transcriptomic data from the scientific literature for morphologically defined neuron types in the rodent hippocampal formation: dentate gyrus, CA3, CA2, CA1, subiculum, and entorhinal cortex. Positive, negative, or mixed expression reports were initially obtained from published articles directly connecting molecular evidence to neurons with known axonal and dendritic patterns across hippocampal layers. Here, we supplement this information by collating, formalizing, and leveraging relational expression inferences that link a gene or protein expression or lack thereof to that of another molecule or to an anatomical location. With these additional interpretations, we freely release online a comprehensive human- and machine-readable molecular profile for more than 100 neuron types in Hippocampome.org. Analysis of these data ascertains the ability to distinguish unequivocally most neuron types in each of the major subdivisions of the hippocampus based on currently known biochemical markers. Moreover, grouping neuron types by expression similarity reveals eight superfamilies characterized by a few defining molecules.
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Mineração de Dados/métodos , Pesquisa Empírica , Hipocampo/fisiologia , Bases de Conhecimento , Neurônios/fisiologia , Transcriptoma/fisiologia , HumanosRESUMO
Most neuroscientists have yet to embrace a culture of data sharing. Using our decade-long experience at NeuroMorpho.Org as an example, we discuss how publicly available repositories may benefit data producers and end-users alike. We outline practical recipes for resource developers to maximize the research impact of data sharing platforms for both contributors and users.
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Disseminação de Informação/métodos , Neurociências , Bases de Dados Factuais , Humanos , Internet , Neurociências/métodos , Neurociências/organização & administraçãoRESUMO
Patterns of periodic voltage spikes elicited by a neuron help define its dynamical identity. Experimentally recorded spike trains from various neurons show qualitatively distinguishable features such as delayed spiking, spiking with or without frequency adaptation, and intrinsic bursting. Moreover, the input-dependent responses of a neuron not only show different quantitative features, such as higher spike frequency for a stronger input current injection, but can also exhibit qualitatively different responses, such as spiking and bursting under different input conditions, thus forming a complex phenotype of responses. In previous work, the comprehensive knowledge base of hippocampal neuron types Hippocampome.org systematically characterized various spike pattern phenotypes experimentally identified from 120 neuron types/subtypes. In this paper, we present a complete set of simple phenomenological models that quantitatively reproduce the diverse and complex phenotypes of hippocampal neurons. In addition to point-neuron models, we created compact multi-compartment models with up to four compartments, which will allow spatial segregation of synaptic integration in network simulations. Electrotonic compartmentalization observed in our compact multi-compartment models is qualitatively consistent with experimental observations. The models were created using an automated pipeline based on evolutionary algorithms. This work maps 120 neuron types/subtypes in the rodent hippocampus to a low-dimensional model space and adds another dimension to the knowledge accumulated in Hippocampome.org. Computationally efficient representations of intrinsic dynamics, along with other pieces of knowledge available in Hippocampome.org, provide a biologically realistic platform to explore the large-scale interactions of various neuron types at the mesoscopic level.
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Potenciais de Ação/fisiologia , Modelos Neurológicos , Neurônios/fisiologia , Animais , Interpretação Estatística de Dados , Bases de Dados Factuais , Hipocampo/metabolismo , Humanos , FenótipoRESUMO
Active neurons can be broadly classified by their intrinsic oscillation patterns into two classes characterized by spiking or bursting. Here, we show that networks of identical bursting neurons with inhibitory pulsatory coupling exhibit itinerant dynamics. Using the relative phases of bursts between neurons, we numerically demonstrate that the network exhibits endogenous transitions between multiple modes of transient synchrony. This is true even for bursts consisting of two spikes. In contrast, our simulations reveal that networks of identical singlet-spiking neurons do not exhibit such complexity. These results suggest a role for bursting dynamics in realizing itinerant complexity in neural circuits.
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Modelos Neurológicos , Neurônios/fisiologia , Potenciais de Ação , Animais , Rede NervosaRESUMO
BACKGROUND: The biomedical literature is expanding at ever-increasing rates, and it has become extremely challenging for researchers to keep abreast of new data and discoveries even in their own domains of expertise. We introduce PaperBot, a configurable, modular, open-source crawler to automatically find and efficiently index peer-reviewed publications based on periodic full-text searches across publisher web portals. RESULTS: PaperBot may operate stand-alone or it can be easily integrated with other software platforms and knowledge bases. Without user interactions, PaperBot retrieves and stores the bibliographic information (full reference, corresponding email contact, and full-text keyword hits) based on pre-set search logic from a wide range of sources including Elsevier, Wiley, Springer, PubMed/PubMedCentral, Nature, and Google Scholar. Although different publishing sites require different search configurations, the common interface of PaperBot unifies the process from the user perspective. Once saved, all information becomes web accessible allowing efficient triage of articles based on their actual relevance and seamless annotation of suitable metadata content. The platform allows the agile reconfiguration of all key details, such as the selection of search portals, keywords, and metadata dimensions. The tool also provides a one-click option for adding articles manually via digital object identifier or PubMed ID. The microservice architecture of PaperBot implements these capabilities as a loosely coupled collection of distinct modules devised to work separately, as a whole, or to be integrated with or replaced by additional software. All metadata is stored in a schema-less NoSQL database designed to scale efficiently in clusters by minimizing the impedance mismatch between relational model and in-memory data structures. CONCLUSIONS: As a testbed, we deployed PaperBot to help identify and manage peer-reviewed articles pertaining to digital reconstructions of neuronal morphology in support of the NeuroMorpho.Org data repository. PaperBot enabled the custom definition of both general and neuroscience-specific metadata dimensions, such as animal species, brain region, neuron type, and digital tracing system. Since deployment, PaperBot helped NeuroMorpho.Org more than quintuple the yearly volume of processed information while maintaining a stable personnel workforce.