Effect of Ivermectin® on survivorship and fertility of Anopheles arabiensis in Ethiopia: an in vitro study.

BACKGROUND: Innovative vector control tools are needed to counteract insecticide resistance and residual malaria transmission. One of such innovative methods is an ivermectin (IVM) treatment to reduce vector survival. In this study, a laboratory experiment was conducted to investigate the effect of ivermectin on survivorship, fertility and egg hatchability rate of Anopheles arabiensis in Ethiopia. METHODS: An in vitro experiment was conducted using 3-5 days old An. arabiensis adults from a colony maintained at insectary of Tropical and Infectious Diseases Research Center, Jimma University (laboratory population) and Anopheles mosquitoes reared from larvae collected from natural mosquito breeding sites (wild population). The mosquitoes were allowed to feed on cattle blood treated with different doses of ivermectin (0 ng/ml, 5 ng/ml, 10 ng/ml, 20 ng/ml, 40 ng/ml and 80 ng/ml). During each feeding experiment, the mosquitoes were held in cages and blood-fed using a Hemotek feeder. Mortality and egg production were then recorded daily for up to 9 days. Time to death was analysed by a Cox frailty model with replicate as frailty term and source of mosquito (wild versus laboratory), treatment type (ivermectin vs control) and their interaction as categorical fixed effects. Kaplan Meier curves were plotted separately for wild and laboratory populations for a visual interpretation of mosquito survival as a function of treatment. RESULTS: Both mosquito source and treatment had a significant effect on survival (P < 0.001), but their interaction was not significant (P = 0.197). Compared to the controls, the death hazard of An. arabiensis that fed on ivermectin-treated blood was 2.3, 3.5, 6.5, 11.5 and 17.9 times that of the control for the 5 ng/ml, 10 ng/ml, 20 ng/ml, 40 ng/ml, and 80 ng/ml dose, respectively. With respect to the number of hatched larvae, hatched pupae and emerged adults per fed mosquitoes, a significant difference was found between the control and the 5 ng/ml dose group (P < 0.001). The number of hatched larvae and pupae, and emerged adults decreased further for the 10 ng/ml dose group and falls to zero for the higher doses. CONCLUSION: Treating cattle blood with ivermectin reduced mosquito survival, fertility, egg hatchability, larval development and adult emergence of An. arabiensis in all tested concentrations of ivermectin in both the wild and laboratory populations. Thus, ivermectin application in cattle could be used as a supplementary vector control method to tackle residual malaria transmission and ultimately achieve malaria elimination in Ethiopia.

Rural community perceptions and practices toward the novel coronavirus (COVID-19) prevention in Konso Zone, Southern Ethiopia: a lesson for the next pandemic.

Background: Corona Virus Disease (COVID-19) has provided a lesson on preparedness and coping mechanisms for similar pandemics to different community segments. To control and prevent the spreading of COVID-19, people need to possess the correct attitude and perception to follow the appropriate practices against the causative agent. Therefore, this study aimed to assess the rural community's perceptions and practices toward COVID-19 prevention among southern Ethiopia's Konso zone rural communities. Methods: A community-based cross-sectional study conducted from April to May 2022 on 605 study participants in the Kena district of the Konso zone of Ethiopia. Participants were recruited using simple random sampling techniques. Face-to-face interviews were conducted to collect data using structured questionnaires developed from the World Health Organization guide and related literature. A logistic regression model was used to identify determinants of perception and practice. Results: Among the 564 participants, 296 (52.5%) believed they would go to the healthcare facility if they contracted COVID-19. About 276 (48.9%) perceived that COVID-19 is not a stigma and should not be concealed. About 233 (41.3%) perceived COVID-19 would be controlled. However, the majority, 451 (80%), 440 (78%), 384 (68.1%), 381 (67.6%), 337 (59.8%), and 317 (56.2%) perceived that the cause of COVID-19 is sin, eating some food items were effective against the virus, no cases in their locality, living far away from COVID-19 area, the virus does not exist, respectively. Overall, only 22.5% of the study participants have good perceptions. About 58.5% practiced handwashing to prevent COVID-19, and 45.2% covered their mouth and nose while coughing/sneezing. Among the participants, 39.8% were vaccinated, and about a quarter (24.8%) of the respondents had good prevention practices. Participants with better educational status, use of social media as a source of information, and those with better income levels are found to be protective factors while being married is a risk factor. Conclusion: The status of the participant's perception and preventive practices toward COVID-19 was meager. There was a substantial magnitude of wrong perceptions about controlling such pandemics. High inaccurate perceptions and low preventive practice need an urgent and sustained improvement strategy to tackle similar pandemics or epidemics.

Indigenous knowledge on the practice of milk container fumigation and its effect on microbial safety of milk among pastoral communities in west Guji zone, southern Ethiopia.

Introduction: Milk is a high-risk food and has been implicated in many foodborne illnesses. Thus, the pastoral communities in rural Ethiopia used a traditional practice of milk container fumigation to maintain the quality and safety of milk. Objective: to assess the indigenous knowledge on milk container fumigation practice and its effect on the microbial safety of milk among pastoral communities in the west Guji zone, southern Ethiopia. Methods: A cross-sectional study design was conducted in six randomly selected kebeles of the pastoral districts in the west Guji zone, Southern Ethiopia from December to June/2022. The preservative plants and raw milk samples were collected and transported to the laboratory, to analyze the efficacy of plants on the microbial safety throughout milk storage. A variance analysis was used to compare the means of microbiological growth and pH measure among the treatments and control; while thematic analysis was for qualitative data. Result: Four species of plants, namely: Olea africana, Clerodendrum myricoides (Hochst) vatke, Rhamnus staddo, and Rhus natalensis were identified from the study area; as they were used for fumigating milk storage containers to prevent a contamination of milk. According to respondents, the fumigation of milk containers was practiced by holding the container upside down over the smoke from a burning chip of each plant species. Accordingly, it was demonstrated that the R. staddo has relatively better efficacy in inhibiting microbial growth in milk than O. africana and C. myricoides (Hochst) vatke; while R. natalensis has no significant impact on microbial growth in milk over the storage period. Conclusion: Pastoralists in the West Guji zone were fumigated the milk storage container by using smoke of O. africana, R. staddo, C. myricoides (Hochst) vatke, and R. natalensis plants. As such, it was identified that R. staddo has relatively better efficacy in inhibiting microbial growth in milk than O. africana and C. myricoides (Hochst) vatke; and it is a better plant to be recommended for the preservation of cow milk.

COVID-19 vaccine acceptance and associated factors among health workers in West Guji zone, Southern Ethiopia: Cross-sectional study.

Background: Currently, different COVID-19 vaccines are being developed and distributed worldwide to increase the proportion of the vaccinated people and as a result to halt the pandemic. However, the vaccination progress is different from place to place even among health care workers due to variation in vaccine acceptance. Therefore, this study aimed to assess the acceptance of COVID-19 vaccine and determinant factors among healthcare workers in west Guji zone, southern Ethiopia. Method and materials: An institutional-based cross-sectional study design was employed to assess COVID-19 vaccine acceptance and associated factors among health care workers from July to August 2021. A simple random sampling technique was used to choose 421 representative healthcare workers from three hospitals in the west Guji Zone. The self-administrated questionnaire was used to collect data. Bivariate and multivariable logistic regression analyses were computed to identify factors associated with the acceptance of the COVID-19 vaccine. P < 0.05 was considered for significantly associated factors. Result: From the representative health workers, 57, 47.02, and 57.9% of healthcare workers had good practice of COVID-19 prevention, adequate knowledge, and a positive attitude toward the COVID-19 vaccine consecutively. 38.1% of healthcare workers said they had a willingness to accept the COVI-19 vaccine. Profession (AOR-6, CI: 2.92-8.22), previous history of vaccine side effects (AOR: 3.67, CI: 2.75-11.41), positive attitude toward vaccine acceptance (AOR: 1.38, CI: 1.18-3.29), adequate knowledge toward COVID-19 vaccine (AOR: 3.33, CI: 1.36-8.12), and adequate practice of COVID-19 prevention measure (AOR: 3.45, CI: 1.39-8.61) were significant associated with COVID-19 vaccine acceptance. Conclusion: The proportion of COVID-19 vaccine acceptance among health workers was found to be low. From the study variables, profession, previous history of vaccine side effects, positive attitude toward vaccine acceptance, adequate knowledge to ward off COVID-19 vaccine, and adequate practice of COVID-19 prevention measures were significantly associated with COVID-19 vaccine acceptance.

Infectious medical waste management during the COVID-19 pandemic in public hospitals of West Guji zone, southern Ethiopia.

Introduction: COVID-19 has swept through the world in a very short period; large volumes of medical waste are being generated in response to the pandemic. Hence, it is imperative to plan and develop evidence-based additional waste management systems. Objective: To assess infectious medical waste management system during the COVID-19 pandemic in public hospitals of the West Guji zone, southern Ethiopia, 2020. Methods: Institution-based cross-sectional study design was conducted from November 05-25/2020. To determine infectious medical waste generation rate, different color plastic buckets and bags was distributed to each ward of the hospitals. Then, its quantity was measured by using a calibrated weighing balance for seven consecutive days. An interviewer-administered questionnaire and an observational checklist were used to collect data related to the existing waste management system, and the knowledge, attitude and practice of waste handlers. The data were analyzed by SPSS version 25 and presented in tables, figures and texts as appropriate. Result: The average infectious medical waste generation rate was determined to be 2.1 kg/bed/day and/or 0.57kg/patient/day. Besides, there was limited segregation of infectious medical waste at the point of generation. Mixed medical waste was collected and transported by using open plastic bin and burned in a brick incinerator or/and dumped in an open field. Moreover, about 42%, 44.6% and 64.8% of the waste handlers had adequate knowledge; a positive attitude and adequate practice respectively. Conclusion: The average infectious medical waste generation rate is above the threshold value (0.2kg/bed/day) set by the WHO. Besides, its management was limited. There was also a gap in the knowledge and attitude of waste handlers towards infectious medical waste management during the COVID-19 pandemic.

Antimicrobial Resistance Profile of Enterobacteriaceae and Drinking Water Quality Among Households in Bule Hora Town, South Ethiopia.

Background: Antimicrobial resistance bacteria and unsafe water are contributing to the growing burden of diseases. Hence, isolation and antimicrobial susceptibility tests for drinking water samples are currently becoming vital to address the human health risks from drinking unsafe water. Objective: This study aimed to assess the antimicrobial resistance profile of Enterobacteriaceae and quality of drinking water among households in Bule Hora Town, South Ethiopia. Methods and Materials: A community-based cross-sectional study was used from June to August 2021. A total of 75 water samples were collected and analyzed at the Microbiological Laboratory of Bule Hora University within 6 hours. A membrane filtration method and biochemical tests were used for the isolation and identification of bacteria. An antimicrobial susceptibility test was performed using Kirby-Bauer disc-diffusion method on Mueller-Hinton Agar. Systematic and simple random probability sampling techniques were used to draw representative samples. The data were entered into Epi Data 3.1 and then exported to SPSS version 25 where univariate and bivariate analyses of the data were conducted. Results: This study indicated that 59 (78.6%), 30 (40%), and 20 (26.6%) of water samples from the storage of households were contaminated with total coliforms, thermo-tolerant coliforms, and Escherichia coli, respectively. Similarly, Salmonella accounted for 18 (24%), Shigella 12 (16%), and other members of the Enterobacteriaceae such as Proteus, Citrobacter, Klebsiella, Escherichia coli, and Enterobacter species were also isolated. All isolates of Enterobacteriaceae showed resistance to amoxicillin plus clavulanic acid and ampicillin. Besides, Shigella showed 10 (83.3%), 2 (16.6%) and 10 (83.3%) and Salmonella showed 16 (88.9%), 5 (27.8%) and 15 (83.3%) resistant to cotrimoxazole, chloramphenicol and tetracycline, respectively. All the tested drinking water samples had no free chlorine at all at point of use. Conclusion: There was contamination of drinking water with coliform and antibiotic-resistant Enterobacteriaceae in most surveyed households.

Why Does COVID-19 Vaccine Acceptance Rate Remain Low Among Patients with Chronic Diseases? Evidences from Public Hospitals of Ethiopia.

Background: Elders and patients with chronic diseases are more vulnerable to coronavirus disease 2019 (COVID-19) infection. In Ethiopia, these groups of people are given vaccination priorities amongst others. However, there is limited information on the acceptance of COVID-19 vaccine among patients with chronic diseases. Therefore, this study aimed to assess the acceptance of COVID-19 vaccine and associated factors among patients with chronic diseases visiting public hospitals in West Guji zone (Ethiopia). Methods and Materials: Facility-based cross-sectional study was employed among 408 chronic disease patients visiting two public hospitals in Ethiopia from September to October 2021. Data was collected using structured questionnaire. Multivariable logistic regression was computed to identify factors associated with acceptance of COVID-19 vaccine. Independent variables with p-value less than 0.05 were considered statistically significantly associated with the acceptance of COVID-19 vaccine. Results: The acceptance of COVID-19 vaccine among patients with chronic diseases was 39.5% (95% CI: 35-44). Factors positively associated with the acceptance of COVID-19 vaccine were age ≥40 years (AOR = 2.84; 95% CI: 1.53-5.28), urban residence (AOR = 0.6; 95% CI: 0.38-0.95), having upper respiratory tract infection (AOR = 1.32; 95% CI: 1.03-2.8), previous COVID-19 infection (AOR = 2.1; 95% CI: 1.22-3.47), having health insurance (AOR = 2.55; 95% CI: 1.23-5.28), those not drinking alcohol (AOR = 1.95; 95% CI: 1.24-3.06), those not chewing Khat (AOR = 2.01; 95% CI: 1.27-3.2), and having good knowledge (AOR = 1.55; 95% CI: 1.03-2.87) and positive attitude (AOR = 2.05; 95% CI: 1.26-3.33) towards the vaccine. Conclusion: The overall proportion of COVID-19 vaccine acceptance is low among patients with chronic diseases. Therefore, to improve the acceptance of COVID-19 vaccine, the government and health professionals should provide continuous health education.

Occupational safety of janitors in Ethiopian University during COVID-19 pandemic: Results from observational study.

Introduction: Janitors are at high risk of COVID-19 infection, as they are among the frontline workers for the prevention and control of COVID-19. Poor occupational safety practices could contribute to loss of lives of janitors and the general public. However, there are no detailed investigations on occupational safety practices of janitors involved in different settings, such as universities where there are crowds of people. In addition, although observation is recognized as a better tool to investigate occupational safety practices, previous studies mainly employed self-administered questionnaires and/or face-to-face interviews as data collection mechanisms. Therefore, this study aimed to assess occupational safety practices to prevent COVID-19 transmission and associated factors among Ethiopian University janitors using an observation tool and a self-administered questionnaire. Methods: An institutional-based cross-sectional study was conducted among 410 janitors of Bule Hora University (Ethiopia) from November to December, 2021. A multivariable logistic regression model was used to identify the independent factors associated with occupational safety practices. Results: Occupational safety practices for COVID-19 were good only among 53.9% of the janitors. Training on COVID-19 prevention measures (AOR = 2.62; 95% CI: 1.57-4.37), availability of policy and protocol in the work place (AOR = 5.46; 95% CI: 3.57-8.36), and availability of soap/bleach (AOR = 2.71; 95% CI: 1.64-4.46) were found to significantly increase the likelihood of occupational safety of the janitors. Conclusion: A significant proportion of the janitors had poor occupational safety practices. Therefore, an adequate supply of PPE and regular training and awareness creation on COVID-19 should be strengthened. Close follow-up and regular supervision of safety procedures should also be conducted as controlling strategies.