A new model for the formation of an Enterococcus faecalis endodontic biofilm with nutritional restriction.

An in vitro model for the formation of an Enterococcus faecalis endodontic biofilm under nutritional restriction was established, simulating clinical conditions for the evaluation of antimicrobial substances. Biofilm formation in dentin was standardized using root quarters incubated with E. faecalis ATCC 29212 at 37°C without nutritional changes. Biofilms were evaluated at 7, 14, and 30 days, counting bacterial colony-forming units using conventional culture and verified scanning electron microscopy. Bacterial viability and biovolume were determined with confocal laser microscopy. Colonization of E. faecalis and biofilm formation on the dentinal surface was confirmed after 7 and 14 days, respectively. Microorganism colonization was homogeneous over the entire root surface at each time point, without significant differences in the viability percentage and biovolume. On the contrary, a decrease in viability and an increase in biovolume were observed when the time was increased. Compared with other incubation times, 14 days was found to be the best time for the establishment of the biofilm in terms of biovolume and bacterial viability. This in vitro model for the formation of endodontic biofilm will allow future evaluation of the efficacy of antimicrobial substances with a more adequate clinical approach.

Risk factors for overnight respiratory events following sedation for magnetic resonance imaging in children with sleep apnea.

PURPOSE: Children with sleep apnea may be at increased risk for overnight respiratory events (ORE) following anesthesia. We sought to identify ORE risk factors in sleep apnea patients sedated for magnetic resonance imaging (MRI). METHODS: One thousand four hundred seven hospitalizations for children with sleep apnea (by ICD-9 code) occurred at our institution from 5/1/2011 to 2/1/2015. One hundred twenty-seven (9 %) encounters were solely for post-MRI observation representing 96 unique patients. The first post-MRI admission for each patient underwent chart review. ORE was defined as sustained oxygen saturation <90 % with need for increased oxygen or adjustment of respiratory support after release from recovery. Characteristics of patients with and without ORE were compared by chi-squared analysis or independent samples t test. Logistic regression identified associations with ORE. RESULTS: Ten out of 96 (10.4 %) patients had ORE. The average time following sedation to ORE was 10.25 h. ORE patients were hospitalized longer (median 2 vs. 1 day, p < 0.001). Overall, patients were 55 % male, 60 % Hispanic, with median age of 5 years [IQR 2-10] and median body mass index (BMI) of 17.9 [IQR 15.2-24]. On logistic regression, apnea-hypopnea index (AHI; OR 1.007 [95 % CI 1.002-1.011]), anesthesia complication (OR 1.13 [95 % CI 1.01-1.28]), and home non-invasive positive pressure ventilation (NIV; OR 6.08 [95 % CI 1.57-26.17]) were associated with ORE. CONCLUSION: Ninety percent of children with sleep apnea admitted for overnight observation following sedated MRI did not have an ORE. AHI, anesthesia complications, and NIV use may help target higher-risk patients and avoid unnecessary hospitalizations.

Method to extract an enhanced cervical vertebrae area from a digital X-ray image.

Combination of digital X-ray with image processing techniques has the potential to extract useful information for healthcare professionals (physicians). From all the information that can be extracted from X-ray images, information concerning the human cervical vertebrae is relevant for the medical area. Therefore, in this work we present a simple enhanced region of interest (ROI) selection tool to select automatically the region that contains most of the information concerning to cervical vertebrae. The ROI-selection method reduces the size of a lateral or frontal digital X-ray by 30-60% without losing significance in the resulting image. This is achieved by an adjustment of dimensions in the image while the cervical area is preserved. Moreover, the visual quality is improved by performing a contrast enhancement in the region of interest. •Automatic threshold selection is computationally more efficient than traditional image segmentation techniques.•Reduce size in comparison with original image (enhancing ROI).•Independence of depth gray scale space.

Lipomatosis intestinal como causa de obstrucción intestinal: reporte de un caso / Intestinal lipomathosys as cause of intestinal obstruction: case report

Se presenta un caso clínico de obstrucción intestinal secundaria a tumor de intestino delgado de origen mesenquimal que requirió resolución quirúrgica, se realiza una revisión de la literatura así como de los métodos diagnósticos utilizados, discutiéndose la utilidad de la terapéutica aplicada en este caso