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1.
Int J Mol Sci ; 24(1)2022 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-36613945

RESUMO

Spinal cord injuries result in the loss of motor and sensory functions controlled by neurons located at the site of the lesion and below. We hypothesized that experimentally enhanced remyelination supports axon preservation and/or growth in the total spinal cord transection in rats. Multifocal demyelination was induced by injection of ethidium bromide (EB), either at the time of transection or twice during transection and at 5 days post-injury. We demonstrated that the number of oligodendrocyte progenitor cells (OPCs) significantly increased 14 days after demyelination. Most OPCs differentiated into mature oligodendrocytes by 60-90 dpi in double-EB-injected rats; however, most axons were remyelinated by Schwann cells. A significant number of axons passed the injury epicenter and entered the distant segments of the spinal cord in the double-EB-injected rats. Moreover, some serotoninergic fibers, not detected in control animals, grew caudally through the injury site. Behavioral tests performed at 60-90 dpi revealed significant improvement in locomotor function recovery in double-EB-injected rats, which was impaired by the blockade of serotonin receptors, confirming the important role of restored serotonergic fibers in functional recovery. Our findings indicate that enhanced remyelination per se, without substantial inhibition of glial scar formation, is an important component of spinal cord injury regeneration.


Assuntos
Doenças Desmielinizantes , Remielinização , Traumatismos da Medula Espinal , Regeneração da Medula Espinal , Ratos , Animais , Axônios/patologia , Regeneração Nervosa/fisiologia , Traumatismos da Medula Espinal/patologia , Medula Espinal/patologia , Etídio , Doenças Desmielinizantes/patologia
2.
Int J Mol Sci ; 22(11)2021 Jun 02.
Artigo em Inglês | MEDLINE | ID: mdl-34199392

RESUMO

Coordination of four-limb movements during quadrupedal locomotion is controlled by supraspinal monoaminergic descending pathways, among which serotoninergic ones play a crucial role. Here we investigated the locomotor pattern during recovery from blockade of 5-HT7 or 5-HT2A receptors after intrathecal application of SB269970 or cyproheptadine in adult rats with chronic intrathecal cannula implanted in the lumbar spinal cord. The interlimb coordination was investigated based on electromyographic activity recorded from selected fore- and hindlimb muscles during rat locomotion on a treadmill. In the time of recovery after hindlimb transient paralysis, we noticed a presence of an unusual pattern of quadrupedal locomotion characterized by a doubling of forelimb stepping in relation to unaffected hindlimb stepping (2FL-1HL) after blockade of 5-HT7 receptors but not after blockade of 5-HT2A receptors. The 2FL-1HL pattern, although transient, was observed as a stable form of fore-hindlimb coupling during quadrupedal locomotion. We suggest that modulation of the 5-HT7 receptors on interneurons located in lamina VII with ascending projections to the forelimb spinal network can be responsible for the 2FL-1HL locomotor pattern. In support, our immunohistochemical analysis of the lumbar spinal cord demonstrated the presence of the 5-HT7 immunoreactive cells in the lamina VII, which were rarely 5-HT2A immunoreactive.


Assuntos
Locomoção/genética , Receptor 5-HT2A de Serotonina/genética , Receptores de Serotonina/genética , Traumatismos da Medula Espinal/genética , Animais , Ciproeptadina/farmacologia , Estimulação Elétrica , Eletromiografia , Membro Anterior/efeitos dos fármacos , Membro Anterior/fisiopatologia , Membro Posterior/efeitos dos fármacos , Membro Posterior/fisiopatologia , Humanos , Locomoção/efeitos dos fármacos , Região Lombossacral/fisiopatologia , Ratos , Receptor 5-HT2A de Serotonina/efeitos dos fármacos , Receptores de Serotonina/efeitos dos fármacos , Serotonina/genética , Serotonina/metabolismo , Antagonistas do Receptor 5-HT2 de Serotonina/farmacologia , Medula Espinal , Traumatismos da Medula Espinal/tratamento farmacológico , Traumatismos da Medula Espinal/patologia , Coluna Vertebral/efeitos dos fármacos , Coluna Vertebral/fisiopatologia
3.
Neural Plast ; 2018: 4232706, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30147717

RESUMO

Serotonin (5-hydroxytryptamine; 5-HT) plays an important role in control of locomotion, partly through direct effects on motoneurons. Spinal cord complete transection (SCI) results in changes in 5-HT receptors on motoneurons that influence functional recovery. Activation of 5-HT2A and 5-HT7 receptors improves locomotor hindlimb movements in paraplegic rats. Here, we analyzed the mRNA of 5-HT2A and 5-HT7 receptors (encoded by Htr2a and Htr7 genes, resp.) in motoneurons innervating tibialis anterior (TA) and gastrocnemius lateralis (GM) hindlimb muscles and the tail extensor caudae medialis (ECM) muscle in intact as well as spinal rats. Moreover, the effect of intraspinal grafting of serotonergic neurons on Htr2a and Htr7 gene expression was examined to test the possibility that the graft origin 5-HT innervation in the spinal cord of paraplegic rats could reverse changes in gene expression induced by SCI. Our results indicate that SCI at the thoracic level leads to changes in Htr2a and Htr7 gene expression, whereas transplantation of embryonic serotonergic neurons modifies these changes in motoneurons innervating hindlimb muscles but not those innervating tail muscles. This suggests that the upregulation of genes critical for locomotor recovery, resulting in limb motoneuron plasticity, might account for the improved locomotion in grafted animals.


Assuntos
Transplante de Tecido Fetal/métodos , Neurônios Motores/metabolismo , Paraplegia/genética , Receptor 5-HT2A de Serotonina/genética , Receptores de Serotonina/genética , Recuperação de Função Fisiológica , Neurônios Serotoninérgicos/transplante , Animais , Transplante de Células , Feminino , Expressão Gênica , Gliose/metabolismo , Membro Posterior/inervação , Locomoção , Músculo Esquelético/inervação , Paraplegia/etiologia , Ratos Wistar , Traumatismos da Medula Espinal/complicações , Vértebras Torácicas
4.
Front Neural Circuits ; 15: 624381, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33981204

RESUMO

Cholinergic and noradrenergic neuromodulation of the synaptic transmission from cortical layer 6 of the primary somatosensory cortex to neurons in the posteromedial thalamic nucleus (PoM) was studied using an in vitro slice preparation from young rats. Cholinergic agonist carbachol substantially decreased the amplitudes of consecutive excitatory postsynaptic potentials (EPSPs) evoked by a 20 Hz five pulse train. The decreased amplitude effect was counteracted by a parallel increase of synaptic frequency-dependent facilitation. We found this modulation to be mediated by muscarinic acetylcholine receptors. In the presence of carbachol the amplitudes of the postsynaptic potentials showed a higher trial-to-trial coefficient of variation (CV), which suggested a presynaptic site of action for the modulation. To substantiate this finding, we measured the failure rate of the excitatory postsynaptic currents in PoM cells evoked by "pseudominimal" stimulation of corticothalamic input. A higher failure-rate in the presence of carbachol indicated decreased probability of transmitter release at the synapse. Activation of the noradrenergic modulatory system that was mimicked by application of norepinephrine did not affect the amplitude of the first EPSP evoked in the five-pulse train, but later EPSPs were diminished. This indicated a decrease of the synaptic frequency-dependent facilitation. Treatment with noradrenergic α-2 agonist clonidine, α-1 agonist phenylephrine, or ß-receptor agonist isoproterenol showed that the modulation may partly rely on α-2 adrenergic receptors. CV analysis did not suggest a presynaptic action of norepinephrine. We conclude that cholinergic and noradrenergic modulation act as different variable dynamic controls for the corticothalamic mechanism of the frequency-dependent facilitation in PoM.


Assuntos
Colinérgicos , Núcleos Talâmicos , Animais , Estimulação Elétrica , Potenciais Pós-Sinápticos Excitadores , Norepinefrina , Ratos , Transmissão Sináptica
5.
Curr Biol ; 31(11): 2347-2358.e6, 2021 06 07.
Artigo em Inglês | MEDLINE | ID: mdl-33848461

RESUMO

Animals display a rich repertoire of defensive responses adequate to the threat proximity. In social species, these reactions can be additionally influenced by the behavior of fearful conspecifics. However, the majority of neuroscientific studies on socially triggered defensive responses focuses on one type of behavior, freezing. To study a broader range of socially triggered reactions and underlying mechanisms, we directly compared two experimental paradigms, mimicking occurrence of the imminent versus remote threat. Observation of a partner currently experiencing aversive stimulation evokes passive defensive responses in the observer rats. Similar interaction with a partner that has just undergone the aversive stimulation prompts animals to increase active exploration. Although the observers display behaviors similar to those of the aversively stimulated demonstrators, their reactions are not synchronized in time, suggesting that observers' responses are caused by the change in their affective state rather than mimicry. Using opsins targeted to behaviorally activated neurons, we tagged central amygdala (CeA) cells implicated in observers' responses to either imminent or remote threat and reactivated them during the exploration of a novel environment. The manipulation revealed that the two populations of CeA cells promote passive or active defensive responses, respectively. Further experiments confirmed that the two populations of cells at least partially differ in expression of molecular markers (protein kinase C-δ [PKC-δ] and corticotropin-releasing factor [CRF]) and connectivity patterns (receiving input from the basolateral amygdala or from the anterior insula). The results are consistent with the literature on single subjects' fear conditioning, suggesting that similar neuronal circuits control defensive responses in social and non-social contexts.


Assuntos
Complexo Nuclear Basolateral da Amígdala , Núcleo Central da Amígdala , Animais , Antígeno Carcinoembrionário , Hormônio Liberador da Corticotropina , Medo , Ratos
6.
Eur J Neurosci ; 32(10): 1715-25, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20964731

RESUMO

In adult mice, classical conditioning in which whisker stimulation is paired with an electric shock to the tail results in a decrease in the frequency of head movements, induces expansion of the cortical representation of stimulated vibrissae and enhances inhibitory synaptic interactions within the 'trained' barrels. We investigated whether such a simple associative learning paradigm also induced changes in neuronal excitability. Using whole-cell recordings from ex vivo slices of the barrel cortex we found that layer IV excitatory cells located in the cortical representation of the 'trained' row of vibrissae had a higher frequency of spikes recorded at threshold potential than neurons from the 'untrained' row and than cells from control animals. Additionally, excitatory cells within the 'trained' barrels were characterized by increased gain of the input-output function, lower amplitudes of fast after-hyperpolarization and decreased effect of blocking of BK channels by iberiotoxin. These findings provide new insight into the possible mechanism for enhanced intrinsic excitability of layer IV excitatory neurons. In contrast, the fast spiking inhibitory cells recorded in the same barrels did not change their intrinsic excitability after the conditioning procedure. The increased excitability of excitatory neurons within the 'trained' barrels may represent the counterpart of homeostatic plasticity, which parallels enhanced synaptic inhibition described previously. Together, the two mechanisms would contribute to increase the input selectivity within the conditioned cortical network.


Assuntos
Potenciais de Ação/fisiologia , Condicionamento Clássico/fisiologia , Neurônios/fisiologia , Córtex Somatossensorial/citologia , 6-Ciano-7-nitroquinoxalina-2,3-diona/farmacologia , Potenciais de Ação/efeitos dos fármacos , Animais , Bicuculina/farmacologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Antagonistas de Receptores de GABA-A/farmacologia , Canais de Potássio Ativados por Cálcio de Condutância Alta/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Neurônios/efeitos dos fármacos , Neurônios/ultraestrutura , Técnicas de Patch-Clamp , Peptídeos/farmacologia , Piperazinas/farmacologia , Bloqueadores dos Canais de Potássio/farmacologia , Córtex Somatossensorial/fisiologia
7.
Neurochem Res ; 35(5): 743-8, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20143157

RESUMO

Neurons are known to accumulate L-carnitine--a compound necessary for transfer of acyl moieties through biological membranes, apart from very low beta-oxidation of fatty acids in adult brain. Present study demonstrates expression of octn2 and octn3 genes coding high affinity carnitine transporters, as well as presence of both proteins in neurons obtained from suckling and adult rats, and also in mouse transformed neural cells. Measurements of carnitine transport show activity of both transporters in neural cells, pointing to their importance in physiological processes other than beta-oxidation.


Assuntos
Neurônios/metabolismo , Proteínas de Transporte de Cátions Orgânicos/biossíntese , Animais , Animais Recém-Nascidos , Carnitina/metabolismo , Linhagem Celular Tumoral , Camundongos , Neuroblastoma/metabolismo , Ratos , Ratos Wistar , Membro 5 da Família 22 de Carreadores de Soluto
8.
Int J Neural Syst ; 29(3): 1850047, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30614324

RESUMO

We have previously shown that during top-down attentional modulation (stimulus expectation) correlations of the beta signals across the primary visual cortex were uniform, while during bottom-up attentional processing (visual stimulation) their values were heterogeneous. These different patterns of attentional beta modulation may be caused by feed-forward lateral inhibitory interactions in the visual cortex, activated solely during stimulus processing. To test this hypothesis, we developed a large-scale computational model of the cortical network. We first identified the parameter range needed to support beta rhythm generation, and next, simulated the different activity states corresponding to experimental paradigms. The model matched our experimental data in terms of spatial organization of beta correlations during different attentional states and provided a computational confirmation of the hypothesis that the paradigm-specific beta activation spatial maps depend on the lateral inhibitory mechanism. The model also generated testable predictions that cross-correlation values depend on the distance between the activated columns and on their spatial position with respect to the location of the sensory inputs from the thalamus.


Assuntos
Atenção/fisiologia , Ritmo beta/fisiologia , Comportamento Compulsivo , Modelos Neurológicos , Inibição Neural/fisiologia , Córtex Visual/fisiologia , Animais , Gatos , Vias Neurais/fisiologia , Sinapses/fisiologia
9.
J Neurosci ; 27(9): 2230-40, 2007 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-17329420

RESUMO

Local field potentials from different visual cortical areas and subdivisions of the cat's lateral posterior-pulvinar complex of the thalamus (LP-P) were recorded during a behavioral task based on delayed spatial discrimination of visual or auditory stimuli. During visual but not auditory attentive tasks, we observed an increase of beta activity (12-25 Hz) as calculated from signals recorded from the caudal part of the lateral zone of the LP-P (LPl-c) as well as from cortical areas 17 and 18 and the complex located at the middle suprasylvian sulcus (MSS). This beta activity appeared only in the trials that ended with a successful response, proving its relationship to the mechanism of visual attention. In contrast, no enhanced beta activity was observed in the rostral part of the lateral zone of the LP-P and in the pulvinar proper. Two subregions of LPl-c (ventromedial and dorsolateral) were distinguished by visually related, attentional beta activity of low (12-18 Hz) and high (18-25 Hz) frequencies, respectively. At the same time, area 17 exhibited attentional activation in the whole beta range, and an increase of power in low-frequency beta was observed in the medial bank of MSS, whereas cortical area 18 and the lateral bank of the MSS were activated in the high beta range. Phase-correlation analysis revealed that two distinct corticothalamic systems were synchronized by the beta activity of different frequencies. One comprised of cortical area 17, ventromedial region of LPl-c, and medial MSS, the second involved area 18 and the dorsolateral LPl-c. Our observations suggest that LPl-c belongs to the wide corticothalamic attentional system, which is functionally segregated by distinct streams of beta activity.


Assuntos
Atenção/fisiologia , Ritmo beta , Córtex Visual/fisiologia , Animais , Gatos , Sinais (Psicologia) , Masculino
10.
PLoS One ; 11(1): e0145379, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26730705

RESUMO

Selective attention can be focused either volitionally, by top-down signals derived from task demands, or automatically, by bottom-up signals from salient stimuli. Because the brain mechanisms that underlie these two attention processes are poorly understood, we recorded local field potentials (LFPs) from primary visual cortical areas of cats as they performed stimulus-driven and anticipatory discrimination tasks. Consistent with our previous observations, in both tasks, we found enhanced beta activity, which we have postulated may serve as an attention carrier. We characterized the functional organization of task-related beta activity by (i) cortical responses (EPs) evoked by electrical stimulation of the optic chiasm and (ii) intracortical LFP correlations. During the anticipatory task, peripheral stimulation that was preceded by high-amplitude beta oscillations evoked large-amplitude EPs compared with EPs that followed low-amplitude beta. In contrast, during the stimulus-driven task, cortical EPs preceded by high-amplitude beta oscillations were, on average, smaller than those preceded by low-amplitude beta. Analysis of the correlations between the different recording sites revealed that beta activation maps were heterogeneous during the bottom-up task and homogeneous for the top-down task. We conclude that bottom-up attention activates cortical visual areas in a mosaic-like pattern, whereas top-down attentional modulation results in spatially homogeneous excitation.


Assuntos
Atenção/fisiologia , Potenciais Evocados Visuais/fisiologia , Desempenho Psicomotor/fisiologia , Córtex Visual/fisiologia , Estimulação Acústica , Animais , Antecipação Psicológica/fisiologia , Gatos , Sinais (Psicologia) , Aprendizagem por Discriminação/fisiologia , Discriminação Psicológica/fisiologia , Estimulação Elétrica , Masculino , Estimulação Luminosa
11.
Mol Biol Cell ; 27(25): 4055-4066, 2016 12 15.
Artigo em Inglês | MEDLINE | ID: mdl-27798233

RESUMO

Synaptic cell adhesion molecules regulate signal transduction, synaptic function, and plasticity. However, their role in neuronal interactions with the extracellular matrix (ECM) is not well understood. Here we report that the CD44, a transmembrane receptor for hyaluronan, modulates synaptic plasticity. High-resolution ultrastructural analysis showed that CD44 was localized at mature synapses in the adult brain. The reduced expression of CD44 affected the synaptic excitatory transmission of primary hippocampal neurons, simultaneously modifying dendritic spine shape. The frequency of miniature excitatory postsynaptic currents decreased, accompanied by dendritic spine elongation and thinning. These structural and functional alterations went along with a decrease in the number of presynaptic Bassoon puncta, together with a reduction of PSD-95 levels at dendritic spines, suggesting a reduced number of functional synapses. Lack of CD44 also abrogated spine head enlargement upon neuronal stimulation. Moreover, our results indicate that CD44 contributes to proper dendritic spine shape and function by modulating the activity of actin cytoskeleton regulators, that is, Rho GTPases (RhoA, Rac1, and Cdc42). Thus CD44 appears to be a novel molecular player regulating functional and structural plasticity of dendritic spines.


Assuntos
Espinhas Dendríticas/fisiologia , Receptores de Hialuronatos/fisiologia , Plasticidade Neuronal/fisiologia , Citoesqueleto de Actina/metabolismo , Animais , Moléculas de Adesão Celular/metabolismo , Células Cultivadas , Células Dendríticas/citologia , Células Dendríticas/fisiologia , Espinhas Dendríticas/metabolismo , Hipocampo/citologia , Receptores de Hialuronatos/genética , Receptores de Hialuronatos/metabolismo , Ácido Hialurônico/metabolismo , Neurônios/citologia , Ratos , Transdução de Sinais/fisiologia , Sinapses/metabolismo , Transmissão Sináptica/fisiologia , Proteínas rho de Ligação ao GTP/metabolismo , Proteína rhoA de Ligação ao GTP/metabolismo
12.
Acta Neurobiol Exp (Wars) ; 72(4): 461-7, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23377275

RESUMO

The second order somatosensory thalamic nucleus (posteromedial nucleus, PoM) receives excitatory projection from layer VI of somatosensory cortex. While it is known that layer VI cortical input to first order, ventrobasal nucleus (VB) is modulated by cholinergic projections from the brainstem, no such data exists concerning the PoM nucleus. In order to study if layer VI corticothalamic transmission to PoM is also modulated we used patch-clamp recording in thalamocortical slices from the rat's brain. Excitatory postsynaptic potentials (EPSPs) were evoked in PoM cells by trains of 5 electrical pulses at 20 Hz frequency applied to corticothalamic fibers. After carbachol was applied to mimic activation of the cholinergic neuromodulatory system corticothalamic EPSP amplitudes were reduced, while facilitation of EPSP amplitudes was enhanced for each next pulse in the series. Such cholinergic control of layer VI corticothalamic synapses in PoM may be used as gain modulator for the transfer of the peripheral sensory information to the cortex.


Assuntos
Vias Aferentes/fisiologia , Colinérgicos/farmacologia , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Núcleos Talâmicos/citologia , Córtex Visual/fisiologia , Vias Aferentes/efeitos dos fármacos , Animais , Biofísica , Carbacol/farmacologia , Estimulação Elétrica , Potenciais Pós-Sinápticos Excitadores/fisiologia , Técnicas In Vitro , Neurônios/fisiologia , Técnicas de Patch-Clamp , Ratos , Ratos Wistar , Sinapses/efeitos dos fármacos , Sinapses/fisiologia , Núcleos Talâmicos/fisiologia
13.
Exp Neurol ; 196(1): 54-72, 2005 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-16085016

RESUMO

The cortico-thalamic influence on spontaneous and visually evoked activity of single cells in the dorsal lateral geniculate (LGN) and perigeniculate (PGN) nuclei were examined in unanesthetized cats with pretrigeminal brainstem transections by means of reversible cooling of cortical areas 17 and 18. The spatio-temporal characteristic of cells' RFs was tested with light spot randomly presented at different points along the receptive field axis. The cessation of cortical input decreased spontaneous activity of most of the LGN cells (64%; as compared to 36% with increased background firing). Similarly, their visually evoked responses were reduced (70% cells; compared to 24% with increased response) and extent of central excitatory domains diminished. In contrast, the majority of PGN neurons increased their spontaneous activity (62%; compared to 38% with decreased firing rate). Cortical cooling resulted also in a decrease of the ON and OFF central responses of most PGN cells (55%; as compared to 20% with increased responses). The described effects were more pronounced within the population of cells in X than in Y pathway. Although the removal of descending cortical excitation disturbed the balance of activity within the network of thalamic cells the gain of the geniculate relay was preserved. We conclude that the main role exerted by the cortico-thalamic pathway serves facilitation of the ascending retino-cortical flow of visual information at the level of lateral geniculate nucleus.


Assuntos
Corpos Geniculados/fisiologia , Neurônios/fisiologia , Córtex Visual/fisiologia , Vias Visuais/fisiologia , Animais , Axotomia , Gatos , Temperatura Baixa , Potenciais Evocados Visuais/fisiologia , Feminino , Masculino
14.
Eur J Neurosci ; 17(2): 421-6, 2003 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-12542680

RESUMO

We have previously proposed that enhanced 16-24 Hz (beta) local field potential activity in the primary visual cortex and lateral geniculate nucleus may be an electrophysiological correlate of the attentional mechanism that increases the gain of afferent visual information flow to the cortex. In this study, we measured coupling between beta signals recorded in the thalamic (i.e. lateral geniculate or perigeniculate) and cortical representations of the central visual field (within 5 degrees from area centralis), during visual and auditory attentive situations. Signal coupling was calculated in two ways: (i) by means of crosscorrelation between raw beta activities, which depends primarily on phase coherence, and (ii) by phase-independent crosscorrelation between amplitude envelopes of beta activities. Mean amplitudes of raw signal cross correlations obtained for thalamo-cortical recording pairs were not significantly different when calculated during behavioural demands for either visual or auditory attention. In contrast, amplitudes of envelope cross correlations obtained during behaviour requiring visual attention were, on average, two times higher than those calculated during the auditory task. This attention-related coupling emerged from synchronized amplitude modulation of beta oscillatory activity that occurs within the cortico-thalamic circuit involved in central vision.


Assuntos
Potenciais Evocados Visuais/fisiologia , Tálamo/fisiologia , Córtex Visual/fisiologia , Percepção Visual/fisiologia , Estimulação Acústica , Animais , Atenção/fisiologia , Ritmo beta , Gatos , Eletrodos Implantados , Estimulação Luminosa
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